Journal of Phycology 44
... express GS type II (Eisenberg et al. 2000). Recently, GS type II was detected in symbiotic plant bacteria (Yuan et al. 2001), but GS type I has not been detected in eukaryotes, so far. Type I and type II forms have some, but not all, of their effectors (parameters that regulate their activity) in co ...
... express GS type II (Eisenberg et al. 2000). Recently, GS type II was detected in symbiotic plant bacteria (Yuan et al. 2001), but GS type I has not been detected in eukaryotes, so far. Type I and type II forms have some, but not all, of their effectors (parameters that regulate their activity) in co ...
Drac1 and Crumbs in amnioserosa morphogenesis
... dorsal closure through effecting cell shape change in the epidermis. Studies in mammalian cells and model organisms have demonstrated the involvement of the Rho subfamily of Ras-related small GTPases in regulation of the actin cytoskeleton and cell shape, and we and others have characterized the inv ...
... dorsal closure through effecting cell shape change in the epidermis. Studies in mammalian cells and model organisms have demonstrated the involvement of the Rho subfamily of Ras-related small GTPases in regulation of the actin cytoskeleton and cell shape, and we and others have characterized the inv ...
Mitochondrial Dynamics and the ER: The Plant Perspective
... Labbé et al., 2014), and thus maintain a certain rate of content exchange. This process was recently shown to be important for fatty acid metabolism in mammalian cells under starvation (Rambold et al., 2015), but is best known for its pivotal role in mitochondrial quality control (Twig et al., 2008b ...
... Labbé et al., 2014), and thus maintain a certain rate of content exchange. This process was recently shown to be important for fatty acid metabolism in mammalian cells under starvation (Rambold et al., 2015), but is best known for its pivotal role in mitochondrial quality control (Twig et al., 2008b ...
Cardioprotective Effects of Erythropoietin in Rats Subjected to
... Administration of erythropoietin (EPO) during or immediately after myocardial ischemia can reduce subsequent myocardial apoptosis, a key phenomenon in myocardial ischemia–reperfusion injury. In this study, we assessed the effect of EPO on 99mTcannexin V myocardial uptake and whether the accumulation ...
... Administration of erythropoietin (EPO) during or immediately after myocardial ischemia can reduce subsequent myocardial apoptosis, a key phenomenon in myocardial ischemia–reperfusion injury. In this study, we assessed the effect of EPO on 99mTcannexin V myocardial uptake and whether the accumulation ...
Fulllength archaeal Rad51 structure and
... biheptameric (thin curve) models indicate a biheptameric ring assembly. Rigid-body re®nement of two heptamers improved the ®t into the experimental data (thick curve). (C) Electron pair distribution functions P(r) for the SAXS data (circles) and for the heptamer (dotted line), biheptamer (thin curve ...
... biheptameric (thin curve) models indicate a biheptameric ring assembly. Rigid-body re®nement of two heptamers improved the ®t into the experimental data (thick curve). (C) Electron pair distribution functions P(r) for the SAXS data (circles) and for the heptamer (dotted line), biheptamer (thin curve ...
Structure and Organelles
... Several organelles are involved in making and processing proteins. • The nucleus stores genetic information. • Many processes occur in the endoplasmic reticulum. • There are two types of endoplasmic reticulum. – rough endoplasmic reticulum – smooth endoplasmic reticulum ...
... Several organelles are involved in making and processing proteins. • The nucleus stores genetic information. • Many processes occur in the endoplasmic reticulum. • There are two types of endoplasmic reticulum. – rough endoplasmic reticulum – smooth endoplasmic reticulum ...
Dependence of an Adenosine-Activated Potassium Current on a
... investigate the cytoplasmic requirementsof the responseof cultured neuronsto adenosine.We have previously shown that in cultured mouse striatal neurons, adenosine activates an inwardly rectifying potassiumconductance(Trusselland Jackson, 1985). We now find that adenosineresponsesin cultured hippocam ...
... investigate the cytoplasmic requirementsof the responseof cultured neuronsto adenosine.We have previously shown that in cultured mouse striatal neurons, adenosine activates an inwardly rectifying potassiumconductance(Trusselland Jackson, 1985). We now find that adenosineresponsesin cultured hippocam ...
Polymorphism in HIV-1 dependency factor PDE8A affects mRNA
... 2008; Yeung et al., 2009; Zhou et al., 2008). Each study identified between 200 and 300 new HDFs (n = 281, 295, 232 and 252 respectively) with only a limited overlap between the sets. As the conditions and readouts of the screens varied between the studies, each screen may have studied host proteins ...
... 2008; Yeung et al., 2009; Zhou et al., 2008). Each study identified between 200 and 300 new HDFs (n = 281, 295, 232 and 252 respectively) with only a limited overlap between the sets. As the conditions and readouts of the screens varied between the studies, each screen may have studied host proteins ...
Avian erythrocytes have functional mitochondria, opening
... its intra-cellular concentration increases during erythrocyte maturation [27], it is likely that methodological issues account for these discrepancies between TEM studies. Indeed, standard TEM preparations are probably not adapted to detect mitochondria without haemoglobin removal. We experimentally ...
... its intra-cellular concentration increases during erythrocyte maturation [27], it is likely that methodological issues account for these discrepancies between TEM studies. Indeed, standard TEM preparations are probably not adapted to detect mitochondria without haemoglobin removal. We experimentally ...
Principles of redox control in photosynthesis gene
... carefully. Light quality effects on the expression of nuclear PS genes have not yet been demonstrated, but such effects do occur in response to changes in light intensity. The expression of Lhcb genes (encoding chlorophyll-binding proteins of lightharvesting complex of PSII [LHCII]) in the unicellul ...
... carefully. Light quality effects on the expression of nuclear PS genes have not yet been demonstrated, but such effects do occur in response to changes in light intensity. The expression of Lhcb genes (encoding chlorophyll-binding proteins of lightharvesting complex of PSII [LHCII]) in the unicellul ...
Distinct roles of doublecortin modulating the microtubule cytoskeleton.
... the doublecortin might protrude from the grooves between protofilaments and that it might therefore impede kinesin movement along the microtubules. Moores and co-workers therefore examined kinesin motion along doublecortinstabilised tracks and found, surprisingly, that doublecortin binding did not p ...
... the doublecortin might protrude from the grooves between protofilaments and that it might therefore impede kinesin movement along the microtubules. Moores and co-workers therefore examined kinesin motion along doublecortinstabilised tracks and found, surprisingly, that doublecortin binding did not p ...
Cholesterol and bile acids regulate xenosensor signaling in
... oligonucleotide was ligated into the EcoRI-site of the pSG5-CXR expression vector. The (UAS)5tk-CAT reporter plasmid was generously provided by Dr. S. A. Kliewer, Department of Molecular Endocrinology, GlaxoSmithKline Research and Development, Research Triangle Park, NC. Oligonucleotides for the wil ...
... oligonucleotide was ligated into the EcoRI-site of the pSG5-CXR expression vector. The (UAS)5tk-CAT reporter plasmid was generously provided by Dr. S. A. Kliewer, Department of Molecular Endocrinology, GlaxoSmithKline Research and Development, Research Triangle Park, NC. Oligonucleotides for the wil ...
- Bronco Scholar
... repeats, concluding that interpretation of the result in this range is uncertain (Snell et al., 1993). However, data has shown that individuals with more than 40 CAG repeats will develop the disease in middle age and those with more than 50 CAG repeats will develop HD as juveniles (Duyao et al., 19 ...
... repeats, concluding that interpretation of the result in this range is uncertain (Snell et al., 1993). However, data has shown that individuals with more than 40 CAG repeats will develop the disease in middle age and those with more than 50 CAG repeats will develop HD as juveniles (Duyao et al., 19 ...
Insightful directed evolution of Escherichia coli quorum sensing
... pTS40 is a plasmid that carries the CloDF13 replication origin (20–40 copies/cell) and under the control of the ampC promoter expresses bicistronic, gfp mut2 and tet (C). This plasmid confers chloramphenicol antibiotic resistance from pTS1 (35). In order to remove the ampC promoter and ampR gene fro ...
... pTS40 is a plasmid that carries the CloDF13 replication origin (20–40 copies/cell) and under the control of the ampC promoter expresses bicistronic, gfp mut2 and tet (C). This plasmid confers chloramphenicol antibiotic resistance from pTS1 (35). In order to remove the ampC promoter and ampR gene fro ...
the depression of phagocytosis by exogenous cyclic nucleotides
... Measurements of Rates of Phagocytosis Ingestion of radioactively labeled starch particles by cellular monolayers was employed as a measure of phagocytic activity as described by Michell et al . (1969) . Approximately 1 .0 X 10 7 cells were used in the preparation of each monolayer resulting in the r ...
... Measurements of Rates of Phagocytosis Ingestion of radioactively labeled starch particles by cellular monolayers was employed as a measure of phagocytic activity as described by Michell et al . (1969) . Approximately 1 .0 X 10 7 cells were used in the preparation of each monolayer resulting in the r ...
Determination of Binding Sites of Cadherin Peptides on the EC1
... bath for 30 sec followed by immediate transfer into ice for 3 min; this process was repeated several times to induce heat shock for permeation of the cDNA vector. Under sterile conditions, 200 L of SOC medium (1.55 g yeast, 0.25 mL of 1 M KCl, 0.5 mL of 1 M MgCl2, 0.5 mL of 1 M MgSO4, 1 mL of 1 M g ...
... bath for 30 sec followed by immediate transfer into ice for 3 min; this process was repeated several times to induce heat shock for permeation of the cDNA vector. Under sterile conditions, 200 L of SOC medium (1.55 g yeast, 0.25 mL of 1 M KCl, 0.5 mL of 1 M MgCl2, 0.5 mL of 1 M MgSO4, 1 mL of 1 M g ...
Continuity And Change: Paradigm Shifts In Neural Induction
... problem.” We will therefore consider (1) how the “soluble inducing factors” have become purified molecules and (2) how knowledge of what these molecules do has changed our traditional ways of thinking about induction. We will focus our attention on the neuralizing property of the organizer, since, u ...
... problem.” We will therefore consider (1) how the “soluble inducing factors” have become purified molecules and (2) how knowledge of what these molecules do has changed our traditional ways of thinking about induction. We will focus our attention on the neuralizing property of the organizer, since, u ...
Arachidonic acid inhibits the store-operated Ca2+ current in rat liver
... response to a variety of hormones [19,26,27]. AA (< 20 µM) in the bath solution failed to activate any current in rat hepatocytes within 10 min of recording (Figure 1B), indicating that the failure to observe any AA-activated current in H4IIE cells is unlikely to be due to the loss of expression of ...
... response to a variety of hormones [19,26,27]. AA (< 20 µM) in the bath solution failed to activate any current in rat hepatocytes within 10 min of recording (Figure 1B), indicating that the failure to observe any AA-activated current in H4IIE cells is unlikely to be due to the loss of expression of ...
Isolation and Characterization of Foaming Proteins of
... molecular weight fraction did not form these complexes appreciably, probably because of its low content of t-amino groups. Immunological studies showed that foaming proteins were formed primarily during germination of barley. The level of foaming proteins decreased considerably during brewing, parti ...
... molecular weight fraction did not form these complexes appreciably, probably because of its low content of t-amino groups. Immunological studies showed that foaming proteins were formed primarily during germination of barley. The level of foaming proteins decreased considerably during brewing, parti ...
Stress response in yeast mRNA export factor
... We further investigated the concentration-dependent effects of ethanol on mRNA export and the change in the localization of Rat8p. As shown in Fig. 4, the localization of GFP-Rat8p partly changed in response to 6% ethanol and completely changed in response to 9% ethanol. Corresponding to this change ...
... We further investigated the concentration-dependent effects of ethanol on mRNA export and the change in the localization of Rat8p. As shown in Fig. 4, the localization of GFP-Rat8p partly changed in response to 6% ethanol and completely changed in response to 9% ethanol. Corresponding to this change ...
Proc. National Academy of Sciences
... enzymes are cell-bound: (i) their activity is detected in extracts after lysozyme-EDTA treatment or brief sonication of washed and pelleted cells but is not detected in the extracellular culture medium (7, 9), (ii) both scanning and transmission electron microscopy reveal eroded concave pits that re ...
... enzymes are cell-bound: (i) their activity is detected in extracts after lysozyme-EDTA treatment or brief sonication of washed and pelleted cells but is not detected in the extracellular culture medium (7, 9), (ii) both scanning and transmission electron microscopy reveal eroded concave pits that re ...
Microfluidic devices for drug discovery and analysis
... have been implicated in several cardiovascular and neuronal disorders (Dunlop et al., 2008). Around 40% of targets in drug discovery belong to the class of ligand-gated ion channels (Yin et al., 2008).They act as the main targets for the currently available pharmaceutical agents, as well as majority ...
... have been implicated in several cardiovascular and neuronal disorders (Dunlop et al., 2008). Around 40% of targets in drug discovery belong to the class of ligand-gated ion channels (Yin et al., 2008).They act as the main targets for the currently available pharmaceutical agents, as well as majority ...
Ribosome Profiling Provides Evidence that Large Please share
... show ribosome profiles that differ from those of the 3′-UTRs of protein-coding genes. Moreover, 5′-UTRs resemble coding regions of protein-coding genes more closely than do lincRNAs. To systematically analyze the pattern of ribosome occupancy across different classes of RNAs, we developed a metric t ...
... show ribosome profiles that differ from those of the 3′-UTRs of protein-coding genes. Moreover, 5′-UTRs resemble coding regions of protein-coding genes more closely than do lincRNAs. To systematically analyze the pattern of ribosome occupancy across different classes of RNAs, we developed a metric t ...
Human pluripotent stem cells
... types from all three embryonic germ layers). While mouse ESCs maintain their pluripotent phenotype when grown on a feeder layer of mouse embryonic fibroblasts (MEFs), Doetschman and colleagues found that, when removed from MEFs and placed into suspension culture with a high fraction of fetal bovine s ...
... types from all three embryonic germ layers). While mouse ESCs maintain their pluripotent phenotype when grown on a feeder layer of mouse embryonic fibroblasts (MEFs), Doetschman and colleagues found that, when removed from MEFs and placed into suspension culture with a high fraction of fetal bovine s ...
Actin-dependent vacuolar occupancy of the cell determines auxin
... seedlings. Auxin treatment (125 nM, 20 h) inhibited expansion in wild-type root epidermal cells (Fig. 2 O, P, and U), but genetic interference with actin and myosin induced a partial resistance to auxin (Fig. 2 Q–U). Notably, mild pharmacological interference with actin, by low doses of LatB (125 nM ...
... seedlings. Auxin treatment (125 nM, 20 h) inhibited expansion in wild-type root epidermal cells (Fig. 2 O, P, and U), but genetic interference with actin and myosin induced a partial resistance to auxin (Fig. 2 Q–U). Notably, mild pharmacological interference with actin, by low doses of LatB (125 nM ...