www.drmichaellevin.org
... from the National Hormone and Pituitary Program) at approximately 0.05 mg/ml in water or PBS. Control beads were soaked in water or PBS only. Beads were implanted between the epiblast and the hypoblast of stage 3/ embryos in New culture (New, 1955) and processed for in situ hybridization at appropri ...
... from the National Hormone and Pituitary Program) at approximately 0.05 mg/ml in water or PBS. Control beads were soaked in water or PBS only. Beads were implanted between the epiblast and the hypoblast of stage 3/ embryos in New culture (New, 1955) and processed for in situ hybridization at appropri ...
Calcium binding to chromaffin vesicle matrix proteins
... Recently we found that Ca2+within chromaffin vesicles is largely bound [Bulenda, D., & Gratzl, M. (1985) Biochemistry 24, 7760-77651. In order to explore the nature of these bonds, we analyzed the binding of Ca2+ to the vesicle matrix proteins as well as to ATP, the main nucleotide present in these ...
... Recently we found that Ca2+within chromaffin vesicles is largely bound [Bulenda, D., & Gratzl, M. (1985) Biochemistry 24, 7760-77651. In order to explore the nature of these bonds, we analyzed the binding of Ca2+ to the vesicle matrix proteins as well as to ATP, the main nucleotide present in these ...
SEMEN ANALYSIS
... • Grade 3: (non-linear motility): These also move forward but tend to travel in a curved or crooked motion. • Grade 2: These have non-progressive motility because they do not move forward despite the fact that they move their tails. • Grade 1: These are immotile and fail to move at all. ...
... • Grade 3: (non-linear motility): These also move forward but tend to travel in a curved or crooked motion. • Grade 2: These have non-progressive motility because they do not move forward despite the fact that they move their tails. • Grade 1: These are immotile and fail to move at all. ...
Biochemical characterization of the vanilloid receptor 1 expressed in
... be localized intracellularly (reviewed in [5]). The investigation of the biochemical characteristics of VR1, like the experimental assessments of its topology and its quaternary structure, its subcellular localization, and its post-translational modifications are just beginning. Recent data obtained ...
... be localized intracellularly (reviewed in [5]). The investigation of the biochemical characteristics of VR1, like the experimental assessments of its topology and its quaternary structure, its subcellular localization, and its post-translational modifications are just beginning. Recent data obtained ...
the fine-tuning of the endomembrane system
... major adjustment of the pathway controlling vacuole formation as well as tonoplasttargeted trafficking. We show that the expression of VPS11 and VPS39, two members of vacuolar tethering complex HOPS, are suppressed in infected cells. This occurs when the infected cells become mature N2-fixing cells. ...
... major adjustment of the pathway controlling vacuole formation as well as tonoplasttargeted trafficking. We show that the expression of VPS11 and VPS39, two members of vacuolar tethering complex HOPS, are suppressed in infected cells. This occurs when the infected cells become mature N2-fixing cells. ...
Genetic and molecular identification of genes
... In the past few years, several efforts have been carried out in order to identify gametophytic mutations. Although the study of several gametophytic mutants allowed the identification of genes involved in embryo sac development ...
... In the past few years, several efforts have been carried out in order to identify gametophytic mutations. Although the study of several gametophytic mutants allowed the identification of genes involved in embryo sac development ...
Have a break: determinants of meiotic DNA double strand break
... and is expressed specifically in germ cells during meiotic prophase. The zinc finger domain has been found to be variable in different mouse strains (and variable in different human populations). It is this variability that allows binding to certain genomic loci with higher or lower affinity, leadin ...
... and is expressed specifically in germ cells during meiotic prophase. The zinc finger domain has been found to be variable in different mouse strains (and variable in different human populations). It is this variability that allows binding to certain genomic loci with higher or lower affinity, leadin ...
The Wnt signaling pathway mechanisms
... translocates into the nucleus to activate transcription of downstream targets together with the transcription factors T cell factor (Tcf) and Lymphoid enhancer factor (Lef). (Arnold et al., 2000; Logan and Nusse, 2004; Nusse, 2005; Cadigan and Peifer, 2009) (figure 1B). The DNA-binding element foun ...
... translocates into the nucleus to activate transcription of downstream targets together with the transcription factors T cell factor (Tcf) and Lymphoid enhancer factor (Lef). (Arnold et al., 2000; Logan and Nusse, 2004; Nusse, 2005; Cadigan and Peifer, 2009) (figure 1B). The DNA-binding element foun ...
Journal of Bacteriology
... Recent literature on the antibiotics enduracidin, moenomycin, prasinomycin, and 11.837 RP suggested an interaction with murein synthesis. Incubation of sensitive strains from Bacillus cereus and Staphylococcus aureus in a "wall medium" containing labeled L-alanine showed that all four antibiotics in ...
... Recent literature on the antibiotics enduracidin, moenomycin, prasinomycin, and 11.837 RP suggested an interaction with murein synthesis. Incubation of sensitive strains from Bacillus cereus and Staphylococcus aureus in a "wall medium" containing labeled L-alanine showed that all four antibiotics in ...
aa-tRNA competition is crucial for the effective translation efficiency
... ribosome usage is a central force in shaping codon usage at the genomic scale [19]. Despite extensive studies, much details of how translation is controlled by mRNA sequences and cellular environment remain exclusive. Both the number of available free ribosomes and the codon orders are important fo ...
... ribosome usage is a central force in shaping codon usage at the genomic scale [19]. Despite extensive studies, much details of how translation is controlled by mRNA sequences and cellular environment remain exclusive. Both the number of available free ribosomes and the codon orders are important fo ...
Journal of Phycology
... lipids in the chloroplast fraction (Fig. 7b, lane 3). Isochrysis galbana and E. huxleyi cultures produced similar results, but E. huxleyi lipids associated with LB were more difficult to recover after centrifugation, even when cells had notable lipid vesicles (not shown). The GC analysis of cell fra ...
... lipids in the chloroplast fraction (Fig. 7b, lane 3). Isochrysis galbana and E. huxleyi cultures produced similar results, but E. huxleyi lipids associated with LB were more difficult to recover after centrifugation, even when cells had notable lipid vesicles (not shown). The GC analysis of cell fra ...
A model of chloroplast growth regulation in mesophyll cells
... chloroplasts have more scatter, which means that chloroplast geometry matters. Here we propose a mechanism to explain this observed chloroplast growth regulation phenomenon. The ability to sense the collective density of one’s own population is found in certain bacteria and is referred to as “quorum ...
... chloroplasts have more scatter, which means that chloroplast geometry matters. Here we propose a mechanism to explain this observed chloroplast growth regulation phenomenon. The ability to sense the collective density of one’s own population is found in certain bacteria and is referred to as “quorum ...
the Early Diagnosis of Prostate Cancer Engrailed-2
... that determine the early identity of cells and tissues (14). We and others have shown that HOX gene dysregulation occurs in most common cancers, with evidence that targeting HOX/PBX binding has therapeutic value (15–19). We have studied Engrailed-2 (En2), another member of this group which show a ve ...
... that determine the early identity of cells and tissues (14). We and others have shown that HOX gene dysregulation occurs in most common cancers, with evidence that targeting HOX/PBX binding has therapeutic value (15–19). We have studied Engrailed-2 (En2), another member of this group which show a ve ...
histology of organogenic and embryogenic responses in cotyledons
... proliferation at the external layers of the root cap and developed mainly following a multicellular budding pattern. Under these culture conditions, we seldom observed secondary embryos originating from cotyledons. Thus, in cork oak, secondary embryogenesis appeared to be associated with the embryo ...
... proliferation at the external layers of the root cap and developed mainly following a multicellular budding pattern. Under these culture conditions, we seldom observed secondary embryos originating from cotyledons. Thus, in cork oak, secondary embryogenesis appeared to be associated with the embryo ...
The Development of an Efficient Somatic Embryogenesis System in
... a, TS of stem internodal section showing intact anatomical features; b, an axillary bud with well developed vascular connection with mother explant; c, embryogenic zones (arrows); d, globular stage with visible protoderm (arrowhead) and a suspensor-like-structure (arrow); e, late globular stage; f, ...
... a, TS of stem internodal section showing intact anatomical features; b, an axillary bud with well developed vascular connection with mother explant; c, embryogenic zones (arrows); d, globular stage with visible protoderm (arrowhead) and a suspensor-like-structure (arrow); e, late globular stage; f, ...
MICROSCOPIC AND FUNCTIONAL STUDIES ON
... endosome is called as a multivesicular body (MVB) (Johnstone, et al. 1987). Multivesicular body may fuse with a lysosome when it and its cargo will be degradated. Another scenario is that the multivesicular body fuses with the plasma membrane (Futter, et al. 1996). In this case the intraluminal vesi ...
... endosome is called as a multivesicular body (MVB) (Johnstone, et al. 1987). Multivesicular body may fuse with a lysosome when it and its cargo will be degradated. Another scenario is that the multivesicular body fuses with the plasma membrane (Futter, et al. 1996). In this case the intraluminal vesi ...
Full Text
... strain were explanted on day 9.5 of gestation (egg cylinder, early neural fold stage) and cultured for 2 days at 38'C (controls), 40 or 41'C. Other heated groups were exposed for 12 or 23 h of the culture period to 40, 40.5 or 41 'C, and for the rest of the culture at 38°C. Culture at 40°C for 2 day ...
... strain were explanted on day 9.5 of gestation (egg cylinder, early neural fold stage) and cultured for 2 days at 38'C (controls), 40 or 41'C. Other heated groups were exposed for 12 or 23 h of the culture period to 40, 40.5 or 41 'C, and for the rest of the culture at 38°C. Culture at 40°C for 2 day ...
Designing logical codon reassignment
... see ref. 19). Unfortunately, the genetic encoding of many spectroscopic probes is oen limited by their complex structure and large size and it may therefore be preferable to introduce them via chemo-selective modication at a genetically-encoded chemical handle (‘tag’) (see previous section). Post- ...
... see ref. 19). Unfortunately, the genetic encoding of many spectroscopic probes is oen limited by their complex structure and large size and it may therefore be preferable to introduce them via chemo-selective modication at a genetically-encoded chemical handle (‘tag’) (see previous section). Post- ...
PDF
... Control of hindbrain segmentation involves several cell signalling pathways. Among them, Fibroblast growth factor (FGF) signalling is necessary in particular to promote Krox20-mediated r3 and r5 development (Aragon and Pujades, 2009; Marin and Charnay, 2000; Maves et al., 2002; Walshe et al., 2002; ...
... Control of hindbrain segmentation involves several cell signalling pathways. Among them, Fibroblast growth factor (FGF) signalling is necessary in particular to promote Krox20-mediated r3 and r5 development (Aragon and Pujades, 2009; Marin and Charnay, 2000; Maves et al., 2002; Walshe et al., 2002; ...
The cell walls of streptococci
... composition as a relatively simple procedure and recent work by others has suggested that cell-wall composition may be of use in classifying streptococci (Roberts & Stewart, 1961 ; Slade & Slamp, 1962). As part of a general study of the taxonomy of the streptococci we have collected over 300 strains ...
... composition as a relatively simple procedure and recent work by others has suggested that cell-wall composition may be of use in classifying streptococci (Roberts & Stewart, 1961 ; Slade & Slamp, 1962). As part of a general study of the taxonomy of the streptococci we have collected over 300 strains ...
processing of defensive pigment in aplysia californica: acquisition
... on the organelle and d is the distance between grid points (in µm). Several control experiments were performed to assess the specificity of the immunostaining and the quality of the immunocytochemical method of phycoerythrin (PE) detection. These included: (1) omission of the primary antiserum (no a ...
... on the organelle and d is the distance between grid points (in µm). Several control experiments were performed to assess the specificity of the immunostaining and the quality of the immunocytochemical method of phycoerythrin (PE) detection. These included: (1) omission of the primary antiserum (no a ...
Spemann`s organizer and the self-regulation of
... dorsal or head structures. Loss of structures can also be triggered by non-specific effects, and therefore ventral genes have received less attention than they deserve. Their investigation may prove a productive area in the future. In conclusion, the Spemann organizer of Xenopus provided a very prod ...
... dorsal or head structures. Loss of structures can also be triggered by non-specific effects, and therefore ventral genes have received less attention than they deserve. Their investigation may prove a productive area in the future. In conclusion, the Spemann organizer of Xenopus provided a very prod ...
PDF
... transcription factors and chromatin by separation of the soluble nuclear fraction from the extraction-resistant fraction, as described (Schreiber et al., 1989) with the following adaptations: after washing with PBS, cells were detached in trypsin (Lonza) and centrifuged at 1500 g for 15 seconds; buf ...
... transcription factors and chromatin by separation of the soluble nuclear fraction from the extraction-resistant fraction, as described (Schreiber et al., 1989) with the following adaptations: after washing with PBS, cells were detached in trypsin (Lonza) and centrifuged at 1500 g for 15 seconds; buf ...