Choosing the Best Kinase Assay to Meet Your Research Needs
... including ion channels, transcription factors, phosphatases and other kinases. Based on sequence analysis, the human genome encodes approximately 518 protein kinases, and approximately one-third of the proteins in a typical mammalian cell are phosphorylated (1). Protein kinases function primarily as ...
... including ion channels, transcription factors, phosphatases and other kinases. Based on sequence analysis, the human genome encodes approximately 518 protein kinases, and approximately one-third of the proteins in a typical mammalian cell are phosphorylated (1). Protein kinases function primarily as ...
Immunity Hemopoiesis and Cellular Drosophila
... 1A). In Drosophila, ush is highly expressed in hemocyte precursors and throughout the development of the plasmatocyte lineage. ush is also expressed in crystal cell precursors but downregulated as these cells develop (18). As in embryos, several signaling pathways associated with hemopoiesis in Dros ...
... 1A). In Drosophila, ush is highly expressed in hemocyte precursors and throughout the development of the plasmatocyte lineage. ush is also expressed in crystal cell precursors but downregulated as these cells develop (18). As in embryos, several signaling pathways associated with hemopoiesis in Dros ...
Methods for imaging and detecting modification of proteins
... prostane pathway [19]. Isoketals are highly electrophilic γ-ketoaldehydes that rapidly and covalently modify lysine residues, forming a variety of adducts including lactam, hydroxylactam, imine, and pyrrole species. The enzymatic oxidation of arachidonic acid also results in the formation of reactiv ...
... prostane pathway [19]. Isoketals are highly electrophilic γ-ketoaldehydes that rapidly and covalently modify lysine residues, forming a variety of adducts including lactam, hydroxylactam, imine, and pyrrole species. The enzymatic oxidation of arachidonic acid also results in the formation of reactiv ...
- ISpatula
... green houses lack insects that make the pollination , so spraying synthetic auxin on greenhouse induces normal fruit development. ...
... green houses lack insects that make the pollination , so spraying synthetic auxin on greenhouse induces normal fruit development. ...
Clear Cell Tumors of the Head and Neck: An
... material in the cytoplasm; hydropic degeneration of organelles and paucity of cellular organelles.1,3 Causes for Physiologic Clearing of Cells The rich glycogen content of the cytoplasm gives a clear cell appearance in remnants of dental lamina,4 rests of malassez 5 and eccrine sweat glands. 6 Neutr ...
... material in the cytoplasm; hydropic degeneration of organelles and paucity of cellular organelles.1,3 Causes for Physiologic Clearing of Cells The rich glycogen content of the cytoplasm gives a clear cell appearance in remnants of dental lamina,4 rests of malassez 5 and eccrine sweat glands. 6 Neutr ...
The effects of extracellular pH on immune function
... pH associated with increases in RNA and protein synthesis and with increased activity of the Na⫹/H⫹ exchanger [12, 13]. In addition, macrophages, neutrophils, and lymphocytes possess two further Cl/HCO3⫺ exchangers, one sodium-dependent and the other sodium-independent [4, 19]. The former is thought ...
... pH associated with increases in RNA and protein synthesis and with increased activity of the Na⫹/H⫹ exchanger [12, 13]. In addition, macrophages, neutrophils, and lymphocytes possess two further Cl/HCO3⫺ exchangers, one sodium-dependent and the other sodium-independent [4, 19]. The former is thought ...
Molecular mechanisms of copper homeostasis in yeast
... Ctr1 is a 406 amino acid integral membrane protein with three putative transmembrane domains. Ctr1 is heavily glycosylated with O-linkages (Dancis et al. 1994b) but it is not known whether glycosylation of Ctr1 is essential for its membrane localization and function. The hydrophilic amino terminal c ...
... Ctr1 is a 406 amino acid integral membrane protein with three putative transmembrane domains. Ctr1 is heavily glycosylated with O-linkages (Dancis et al. 1994b) but it is not known whether glycosylation of Ctr1 is essential for its membrane localization and function. The hydrophilic amino terminal c ...
5.3 G Protein-Coupled Receptors
... The receptor–G-protein complex remains the only major G protein conformation for which atomic-scale structural information is unavailable. In the resting state, G proteins are heterotrimers of GDP-bound α- (blue), β- (green) and γ- (yellow) subunits (Gαt/iβ1γ1). On binding of an extracellular stimul ...
... The receptor–G-protein complex remains the only major G protein conformation for which atomic-scale structural information is unavailable. In the resting state, G proteins are heterotrimers of GDP-bound α- (blue), β- (green) and γ- (yellow) subunits (Gαt/iβ1γ1). On binding of an extracellular stimul ...
Processing of human cytomegalovirus envelope glycoproteins in
... content of O-linked oligosaccharides (Kari & Gehrz, 1988). Furthermore, by using monensin to prevent posttranslational processing in the Golgi network of SF cells, in which O-glycosylation occurs, the Mr of gp47-63 was reduced by 20000 (Gretch et al., 1988b). This decrease was similar to that observ ...
... content of O-linked oligosaccharides (Kari & Gehrz, 1988). Furthermore, by using monensin to prevent posttranslational processing in the Golgi network of SF cells, in which O-glycosylation occurs, the Mr of gp47-63 was reduced by 20000 (Gretch et al., 1988b). This decrease was similar to that observ ...
Universal Microfluidic System for Analysis and Control of Cell
... thousands of formulated fluids to any designate on-chip culture unit, while monitoring and analyzing corresponding cellular responses via live cell microscopy and end-point biochemical or genetic analysis methods. This microfluidic system is a powerful platform for both fundamental and translational ...
... thousands of formulated fluids to any designate on-chip culture unit, while monitoring and analyzing corresponding cellular responses via live cell microscopy and end-point biochemical or genetic analysis methods. This microfluidic system is a powerful platform for both fundamental and translational ...
Coordinate expression of matrix-degrading proteinases and their
... sons et al., 1997). The MMP gene expression also is regulated and is influenced by multiple cytokines and growth factors (Emonard and Grimaud, 1990; Ries and Petrides, 1995). The role of gene expression in regulation of the alternative zymogen activation cascades is less clear, but a few published r ...
... sons et al., 1997). The MMP gene expression also is regulated and is influenced by multiple cytokines and growth factors (Emonard and Grimaud, 1990; Ries and Petrides, 1995). The role of gene expression in regulation of the alternative zymogen activation cascades is less clear, but a few published r ...
Evidence for particleinduced horizontal gene transfer and serial
... Incubation of the amino acid-deficient strain Escherichia coli AB1157 with particles harvested from an oligotrophic environment revealed evidence of horizontal gene transfer (HGT) with restoration of all deficiencies in revertant cells with frequencies up to 1.94 105. None of the markers were pre ...
... Incubation of the amino acid-deficient strain Escherichia coli AB1157 with particles harvested from an oligotrophic environment revealed evidence of horizontal gene transfer (HGT) with restoration of all deficiencies in revertant cells with frequencies up to 1.94 105. None of the markers were pre ...
The Expression of Slow Myosin During Mammalian Somitogenesis
... At the indicated periods of culture, cells were fixed with 50% ethanol/50% acetone at -20°C for 10 min, washed in PBS, and incubated with the antimyosin monoclonal or polyclonal antibodies (at appropriate dilutions for 30 rain at 25°C) and then with a goat anti-mouse Ig conjugated with rhodemine or ...
... At the indicated periods of culture, cells were fixed with 50% ethanol/50% acetone at -20°C for 10 min, washed in PBS, and incubated with the antimyosin monoclonal or polyclonal antibodies (at appropriate dilutions for 30 rain at 25°C) and then with a goat anti-mouse Ig conjugated with rhodemine or ...
Lipids as hormones and second messengers
... differentiation factors, etc.) to membrane receptors triggers a sequence of events that usually involves cleavage of a membrane lipid to a bioactive fragment or derivative. Examples of the lipases that are involved in these events are as follows. Phospholipase A 2 (PLA 2) releases a (usually polyuns ...
... differentiation factors, etc.) to membrane receptors triggers a sequence of events that usually involves cleavage of a membrane lipid to a bioactive fragment or derivative. Examples of the lipases that are involved in these events are as follows. Phospholipase A 2 (PLA 2) releases a (usually polyuns ...
Plant Phosphoglycerolipids: The Gatekeepers of Vascular Cell
... et al., 2004; Kobayashi et al., 2011). The GPI anchor is made of one molecule of phosphatidylinositol to which a carbohydrate chain is linked through the 60 hydroxyl of the inositol, and is linked to the protein through an ethanolamine phosphate moiety (Gruenberg, 2001). In addition to this domain, ...
... et al., 2004; Kobayashi et al., 2011). The GPI anchor is made of one molecule of phosphatidylinositol to which a carbohydrate chain is linked through the 60 hydroxyl of the inositol, and is linked to the protein through an ethanolamine phosphate moiety (Gruenberg, 2001). In addition to this domain, ...
Genetic Control of Programmed Cell Death in the Nematode C
... ted-3 mutation acts prior to ted-7 to block the initiation of programmed cell death. Mutations in ted-3 block the programmed cell deaths that occur during embryonic development as well as those that occur during postembryonic development. We have screened ted-7; ted-3 hermaphrodites of various stage ...
... ted-3 mutation acts prior to ted-7 to block the initiation of programmed cell death. Mutations in ted-3 block the programmed cell deaths that occur during embryonic development as well as those that occur during postembryonic development. We have screened ted-7; ted-3 hermaphrodites of various stage ...
Plant Cell Growth and Elongation
... potatoes or metaxylem elements, the final cell size can be 100 000 or 1 000 000 times greater than the original size of the cell, when born in the meristem. Most of the volumetric increase in the protoplast is accounted for by expansion of the vacuole (up to 95% cell volume). The existing cell wall a ...
... potatoes or metaxylem elements, the final cell size can be 100 000 or 1 000 000 times greater than the original size of the cell, when born in the meristem. Most of the volumetric increase in the protoplast is accounted for by expansion of the vacuole (up to 95% cell volume). The existing cell wall a ...
Human cytomegalovirus reactivation from latency
... differentiation of monocytes into macrophages in vivo could represent a key event triggering the reactivation of the latent virus, giving rise to a productive infection and allowing HCMV to disseminate into host tissues [22, 25, 31, 33–35]. On that basis, a number of experimental models have been de ...
... differentiation of monocytes into macrophages in vivo could represent a key event triggering the reactivation of the latent virus, giving rise to a productive infection and allowing HCMV to disseminate into host tissues [22, 25, 31, 33–35]. On that basis, a number of experimental models have been de ...
Peptide inhibitors of the essential cell division protein FtsA
... process depends on recruitment of specific proteins in an absolutely essential cascade forming the divisome (Dai and Lutkenhaus, 1992; Projan, 2002; Errington et al., 2003). To our knowledge, the intrinsic cell division inhibitors MinC (Errington et al., 2003) and SulA (Cordell et al., 2003) which c ...
... process depends on recruitment of specific proteins in an absolutely essential cascade forming the divisome (Dai and Lutkenhaus, 1992; Projan, 2002; Errington et al., 2003). To our knowledge, the intrinsic cell division inhibitors MinC (Errington et al., 2003) and SulA (Cordell et al., 2003) which c ...
In Search of Mitochondrial Mechanisms: Interfield
... his claim that quite different images were produced by exemplars with the same long, narrow shape depending on the angle at which each happened to be sliced. If so, some of the bafflingly diverse forms within the microscopic image of a single cell could be subsumed under a single category. By the turn ...
... his claim that quite different images were produced by exemplars with the same long, narrow shape depending on the angle at which each happened to be sliced. If so, some of the bafflingly diverse forms within the microscopic image of a single cell could be subsumed under a single category. By the turn ...
Kinetics of antiviral CD8 T cell responses during primary and post
... viral clearance, but did result in more serious symptoms. Consistent with a key role of the CD8 T cell response, Mclnnes et al. [16] showed that acute bRSV infection in cattle is associated with an influx of activated CD8 T cells into the lungs and the trachea, peaking at Day 10 post-infection. On t ...
... viral clearance, but did result in more serious symptoms. Consistent with a key role of the CD8 T cell response, Mclnnes et al. [16] showed that acute bRSV infection in cattle is associated with an influx of activated CD8 T cells into the lungs and the trachea, peaking at Day 10 post-infection. On t ...
Chapter 3—Cells
... Which of the following statements is not part of the cell theory? Animals and plants share the same kinds of cells. All organisms are made up of one or more cells. The cell is the basic unit of all living things. All cells come from existing cells. ...
... Which of the following statements is not part of the cell theory? Animals and plants share the same kinds of cells. All organisms are made up of one or more cells. The cell is the basic unit of all living things. All cells come from existing cells. ...
Integrated Network Analysis of Genetic and Epigenetic factors in
... common alleles with common diseases have mainly focused on the investigation of variants individually. However, due to their rarity, the frequencies of rare alleles may be comparable with genotyping errors. As a consequence, individual tests of association of rare variants with disease, as is often ...
... common alleles with common diseases have mainly focused on the investigation of variants individually. However, due to their rarity, the frequencies of rare alleles may be comparable with genotyping errors. As a consequence, individual tests of association of rare variants with disease, as is often ...