tethering redox proteins to the outer membrane in Neisseria and
... [15]. This yields a mature peptide with an N-terminal cysteine followed by an extended LCR (low-complexity region), which is in turn followed by a protein domain with high identity with the globular domains of homologous nitrite reductase, azurin and CCP respectively. {We have excluded cytochrome c ...
... [15]. This yields a mature peptide with an N-terminal cysteine followed by an extended LCR (low-complexity region), which is in turn followed by a protein domain with high identity with the globular domains of homologous nitrite reductase, azurin and CCP respectively. {We have excluded cytochrome c ...
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... studies of their development in clonal cultures (e.g. Rutz and Hauschka, 1982) or by transplanting them singly to new positions in the embryo (Wylie et al. 1987). Commitment could depend upon the activity of relatively few specific regulatory 'lineage switching' genes, for example the MyoD gene (Dav ...
... studies of their development in clonal cultures (e.g. Rutz and Hauschka, 1982) or by transplanting them singly to new positions in the embryo (Wylie et al. 1987). Commitment could depend upon the activity of relatively few specific regulatory 'lineage switching' genes, for example the MyoD gene (Dav ...
Intrinsically Disordered Proteins (IDPs)
... – Predicting IDPs from amino acid sequence – Some important results from IDP prediction – An improved order / disorder amino acid scale – Predicting phosphorylation sites – Disorder and function: two examples ...
... – Predicting IDPs from amino acid sequence – Some important results from IDP prediction – An improved order / disorder amino acid scale – Predicting phosphorylation sites – Disorder and function: two examples ...
Listeria Nontoxic radioactive is a highly effective therapy against metastatic pancreatic cancer
... of RL/500 μL of saline (group 1), 104 cfu of Listeriaat/500 μL of saline (group 2), 200 μCi of 188Re/500 μL of saline (group 3), or 500 μL of saline (group 4) on days 3–9, followed by a rest period of 1 wk, followed by four injections with the same dose on days 16–19. All mice were euthanized on day ...
... of RL/500 μL of saline (group 1), 104 cfu of Listeriaat/500 μL of saline (group 2), 200 μCi of 188Re/500 μL of saline (group 3), or 500 μL of saline (group 4) on days 3–9, followed by a rest period of 1 wk, followed by four injections with the same dose on days 16–19. All mice were euthanized on day ...
Development of Peltate Glandular Trichomes of
... al., 2000). These results demonstrate coincidental temporal changes in enzyme activities, enzyme protein level, and steady-state transcript abundances and indicate that most of the monoterpene biosynthetic enzymes in peppermint oil glands are developmentally regulated at the level of gene expression ...
... al., 2000). These results demonstrate coincidental temporal changes in enzyme activities, enzyme protein level, and steady-state transcript abundances and indicate that most of the monoterpene biosynthetic enzymes in peppermint oil glands are developmentally regulated at the level of gene expression ...
DNA asymmetry in stem cells – immortal or mortal?
... segregation’ does not necessarily describe a single biological phenomenon. As such, each type of non-random sister chromatid segregation can have a distinct biological meaning, and be carried out through distinct molecular or cellular mechanisms. Non-random sister chromatid segregation during stem c ...
... segregation’ does not necessarily describe a single biological phenomenon. As such, each type of non-random sister chromatid segregation can have a distinct biological meaning, and be carried out through distinct molecular or cellular mechanisms. Non-random sister chromatid segregation during stem c ...
Limitations of Allotopic Expression of Mitochondrial Genes in
... allotopically. Analyses of the hydrophobic patterns of different polypeptides suggest that hydrophobicity of the N-terminal segment is the main determinant for the importability of peptides into mammalian mitochondria. ...
... allotopically. Analyses of the hydrophobic patterns of different polypeptides suggest that hydrophobicity of the N-terminal segment is the main determinant for the importability of peptides into mammalian mitochondria. ...
Mechanisms of vertebrate segmentation
... cells emerging from this part of the streak also contribute to the notochord (Spratt, 1955; Rosenquist, 1966; Nicolet, 1971; Tarn & Beddington, 1987). As development proceeds, neurulation takes place in the midline, and the segmental plates come to flank the neural tube and notochord (Figs 1A, 2). I ...
... cells emerging from this part of the streak also contribute to the notochord (Spratt, 1955; Rosenquist, 1966; Nicolet, 1971; Tarn & Beddington, 1987). As development proceeds, neurulation takes place in the midline, and the segmental plates come to flank the neural tube and notochord (Figs 1A, 2). I ...
Phorbol Esters Alter Cell Fate during Development of Sea Urchin
... The morphological changes observed during gastrulation as a result of treatment with phorbol ester during cleavage led us to investigate the changes in gene expression in TPAtreated embryos, as assessed by RNA accumulation. A number of genes have been isolated in sea urchins that by the early gastru ...
... The morphological changes observed during gastrulation as a result of treatment with phorbol ester during cleavage led us to investigate the changes in gene expression in TPAtreated embryos, as assessed by RNA accumulation. A number of genes have been isolated in sea urchins that by the early gastru ...
DIFFERENCES IN PLOIDY AND DEGREE OF INTERCELLULAR
... usually considered to be possible only immediately following cell division (Sinnott & Bloch, 1945; Fosket, 1968). In some instances, however, differentiation can be induced in parenchyma cells without immediate prior cytokinesis (Sachs, 1969). Fosket & Torrey (1969) have used soybean tissue to show ...
... usually considered to be possible only immediately following cell division (Sinnott & Bloch, 1945; Fosket, 1968). In some instances, however, differentiation can be induced in parenchyma cells without immediate prior cytokinesis (Sachs, 1969). Fosket & Torrey (1969) have used soybean tissue to show ...
The role of lipids in the biogenesis of integral membrane
... transport/diffusion (Bogdanov and Dowhan 1995). Loss of full transporter function of LacY in cells lacking PE correlates with a structural alteration in the periplasmic domain P7 of LacY, as indicated by a loss of recognition by a conformation-sensitive monoclonal antibody (Fig. 1). PE is not requir ...
... transport/diffusion (Bogdanov and Dowhan 1995). Loss of full transporter function of LacY in cells lacking PE correlates with a structural alteration in the periplasmic domain P7 of LacY, as indicated by a loss of recognition by a conformation-sensitive monoclonal antibody (Fig. 1). PE is not requir ...
Dynamic Interplay between Nucleoid Segregation
... damaged chloroplast DNA and by suppressing aberrant recombination between short dispersed repeats in the moss Physcomitrella patens. Here, overexpression and knockdown analysis of cpRECA in the green alga Chlamydomonas reinhardtii revealed that cpRECA was involved in cp nucleoid dynamics as well as ...
... damaged chloroplast DNA and by suppressing aberrant recombination between short dispersed repeats in the moss Physcomitrella patens. Here, overexpression and knockdown analysis of cpRECA in the green alga Chlamydomonas reinhardtii revealed that cpRECA was involved in cp nucleoid dynamics as well as ...
Select Acetophenones Modulate Flagellar Motility in Chlamydomonas
... sought to identify novel chemical inhibitors of motility. Accordingly, we screened a chemical library of acetophenones (all obtained from Sigma-Aldrich) for inhibitory effects on ciliary motility. Acetophenones were selected for their commercial availability and generally low toxicity in eukaryotic ...
... sought to identify novel chemical inhibitors of motility. Accordingly, we screened a chemical library of acetophenones (all obtained from Sigma-Aldrich) for inhibitory effects on ciliary motility. Acetophenones were selected for their commercial availability and generally low toxicity in eukaryotic ...
Secretion of a murine retroviral Env associated with resistance to
... peculiar to the Fv4 Env, we transfected NIH3T3 cells with a vector encoding the MoMLV Env (Matano et al., 1993), isolated three Env-expressing clones and compared the synthesis and processing of envelope glycoproteins in these cells to that in tail cells from Fv4-transgenic mice and FrMLVinfected NI ...
... peculiar to the Fv4 Env, we transfected NIH3T3 cells with a vector encoding the MoMLV Env (Matano et al., 1993), isolated three Env-expressing clones and compared the synthesis and processing of envelope glycoproteins in these cells to that in tail cells from Fv4-transgenic mice and FrMLVinfected NI ...
involvement of protein tyrosine phosphorylation of human sperm in
... stimulating factor-1 (CSF-1) (30), interferon-γ (IFN-γ ) (35), tumor necrosis factor-α (TNF-α) (34), epidermal growth factor (EGF) (37), thymosin α1 (Tα1) (9), thymosin ß4 (Tß4) (9); and several unidentified factors (29). Some of these cytokines are also present in cervical mucus of women (38, 39), ...
... stimulating factor-1 (CSF-1) (30), interferon-γ (IFN-γ ) (35), tumor necrosis factor-α (TNF-α) (34), epidermal growth factor (EGF) (37), thymosin α1 (Tα1) (9), thymosin ß4 (Tß4) (9); and several unidentified factors (29). Some of these cytokines are also present in cervical mucus of women (38, 39), ...
Ploidy of Bacillus subtilis exfusants: the haploid nature of cells
... transferred with toothpicks onto three minimal TS media containing 5 pg DNAase I ml-l; two plates, the 'parental' ones, were supplemented with requirements of one or the other of the partners (Fig. 1). After overnight incubation at 37 "C, colonies were classified following criteria given in Fig. 1. ...
... transferred with toothpicks onto three minimal TS media containing 5 pg DNAase I ml-l; two plates, the 'parental' ones, were supplemented with requirements of one or the other of the partners (Fig. 1). After overnight incubation at 37 "C, colonies were classified following criteria given in Fig. 1. ...
Distinct signals in human immunodeficiency virus type 1 Pr55
... The human immunodeficiency virus (HIV) groupspecific antigen (gag) gene product represents the major component of the virus particle. It is first synthesized as a precursor protein of 55K (Pr55) and forms the structural basis of the retrovirus core. During or following virus release, Pr55 is cleaved ...
... The human immunodeficiency virus (HIV) groupspecific antigen (gag) gene product represents the major component of the virus particle. It is first synthesized as a precursor protein of 55K (Pr55) and forms the structural basis of the retrovirus core. During or following virus release, Pr55 is cleaved ...
The Plant Journal
... by Kang et al. (2001) suggested that ADL1 is associated with the cell plate. ADL2 was localized to the plastids (Kang et al., 1998). ADL6 was shown to associate with the Golgi apparatus (Jin et al., 2001). The cellular compartment to which ADL3 associates is currently unknown. Apart from difference ...
... by Kang et al. (2001) suggested that ADL1 is associated with the cell plate. ADL2 was localized to the plastids (Kang et al., 1998). ADL6 was shown to associate with the Golgi apparatus (Jin et al., 2001). The cellular compartment to which ADL3 associates is currently unknown. Apart from difference ...
AGING Dr Aubrey de Grey Chief Science Officer
... 2) Identify the enzymes (mutagenesis, chemistry, genomics). 3) Make lysosome-targeted transgenes; assay cell toxicity. 4) Assay competence in vitro (more mutagenesis/selection). ...
... 2) Identify the enzymes (mutagenesis, chemistry, genomics). 3) Make lysosome-targeted transgenes; assay cell toxicity. 4) Assay competence in vitro (more mutagenesis/selection). ...
Asymmetries in Cell Division, Cell Size, and Furrowing in the
... 1986). The resulting shear modifies the pigment distribution at the equator, producing a gray crescent, which corresponds to the embryo presumptive dorsal side. The gray crescent is also observed in some other amphibian species such as Rana pipiens. Although the position of the SEP, which can be sim ...
... 1986). The resulting shear modifies the pigment distribution at the equator, producing a gray crescent, which corresponds to the embryo presumptive dorsal side. The gray crescent is also observed in some other amphibian species such as Rana pipiens. Although the position of the SEP, which can be sim ...
5-Breast Cancer immunohistochemistry
... functioning ER. Some investigators have found that Progesterone status has a greater power than ER status in predicting the disease free interval. It can be determined in FFPE tissue using similar methods to ER detection and evaluated in the same way. Between 55% and 65% of breast cancers are likely ...
... functioning ER. Some investigators have found that Progesterone status has a greater power than ER status in predicting the disease free interval. It can be determined in FFPE tissue using similar methods to ER detection and evaluated in the same way. Between 55% and 65% of breast cancers are likely ...
Cells are Either Prokaryotic or Eukaryotic
... • Materials move from one structure to another in the cell via the endomembrane system. • Here a piece of membrane, with proteins or other materials inside, can bud off from one organelle, move through the cell, and then fuse with another membrane-lined structure. ...
... • Materials move from one structure to another in the cell via the endomembrane system. • Here a piece of membrane, with proteins or other materials inside, can bud off from one organelle, move through the cell, and then fuse with another membrane-lined structure. ...
a biological “arms race” - Open Access Journals at BC
... III. Interaction between APOBEC3 and Vif Soon after the discovery of HIV-1, the viral protein termed viral infectivity factor (Vif) was found to be necessary for successful infection.24 However, it was not necessary in every cell type; HIV-1Δvif could infect certain permissive cell lines, while infe ...
... III. Interaction between APOBEC3 and Vif Soon after the discovery of HIV-1, the viral protein termed viral infectivity factor (Vif) was found to be necessary for successful infection.24 However, it was not necessary in every cell type; HIV-1Δvif could infect certain permissive cell lines, while infe ...
Epstein-Barr virus-Associated Lymphomas in Malaysia: High
... DNA replication and activation of the transcription of EBV genes, as well as in the transformation and the immortalization of Blymphocytes . BNLF1, which encodes for LMP-1, is not only essential for B-cell transformation ; it is also the only EBV gene that has transforming effects in non-lymphoid ce ...
... DNA replication and activation of the transcription of EBV genes, as well as in the transformation and the immortalization of Blymphocytes . BNLF1, which encodes for LMP-1, is not only essential for B-cell transformation ; it is also the only EBV gene that has transforming effects in non-lymphoid ce ...
Signal Requirements for the Generation of CD4+ and CD8+ T
... effector cells and whether EC alloantigens were actually involved in the generation of the response, multiple genetically unrelated EC lines were used as targets in the final cytotoxicity assay, in addition to the NKsensitive line, K562. The results (Figures 2a and 2b) demonstrate that the two most ...
... effector cells and whether EC alloantigens were actually involved in the generation of the response, multiple genetically unrelated EC lines were used as targets in the final cytotoxicity assay, in addition to the NKsensitive line, K562. The results (Figures 2a and 2b) demonstrate that the two most ...