Comparison of an immortalized human corneal epithelial cell line
... Susceptibility of HCE to HSV-1 and its suitability for the isolation of HSV-1 The HCE was found to be suceptible to HSV-1 as determined by both the shell vial and tube cultures. Cytopathic effect (CPE) (fig. 2) was seen in the tube cultures at 48 h (>50% of the cells were refractile, showed rounding ...
... Susceptibility of HCE to HSV-1 and its suitability for the isolation of HSV-1 The HCE was found to be suceptible to HSV-1 as determined by both the shell vial and tube cultures. Cytopathic effect (CPE) (fig. 2) was seen in the tube cultures at 48 h (>50% of the cells were refractile, showed rounding ...
Regenerative medicine in dermatology: biomaterials, tissue
... domains of Suprathel (PolyMedics Innovations GmbH, Denkendorf, Germany) are second-degree burns and donor sites of skin transplantation. Promising advantages are a rapid painless re-epithelialization and the possibility of application next to utilized regions like joints (31). Being engineered alre ...
... domains of Suprathel (PolyMedics Innovations GmbH, Denkendorf, Germany) are second-degree burns and donor sites of skin transplantation. Promising advantages are a rapid painless re-epithelialization and the possibility of application next to utilized regions like joints (31). Being engineered alre ...
Cells
... Overview of Cellular Respiration Organisms obtain energy in a process called cellular respiration. The equation for cellular respiration is the opposite of the equation for ...
... Overview of Cellular Respiration Organisms obtain energy in a process called cellular respiration. The equation for cellular respiration is the opposite of the equation for ...
A Hierarchy of Regulatory Genes Controls a Larva-to-Adult
... of cell type in yeast (Rine et al., 1981; Mitchell and Herskowitz, 1986; Kassir et al., 1988). Epistasis analysis provides a genetic formalism to describe the functional nature of interactions among genes without presupposing any particular molecular mechanisms for those interactions. For example, p ...
... of cell type in yeast (Rine et al., 1981; Mitchell and Herskowitz, 1986; Kassir et al., 1988). Epistasis analysis provides a genetic formalism to describe the functional nature of interactions among genes without presupposing any particular molecular mechanisms for those interactions. For example, p ...
Cell distribution and regenerative activity following meniscus
... term, long-term follow-up indicates that meniscal removal provides an increased risk of osteoarthritis [3, 4]. While meniscal repair is growing in popularity, its role is relatively limited given the inherent low vascularity of the inner zones of the meniscus [5, 6]. Furthermore, repair in the outer ...
... term, long-term follow-up indicates that meniscal removal provides an increased risk of osteoarthritis [3, 4]. While meniscal repair is growing in popularity, its role is relatively limited given the inherent low vascularity of the inner zones of the meniscus [5, 6]. Furthermore, repair in the outer ...
Primary Mediastinal Seminomas: Evidence of Single KIT
... unique (Figs. 3 and 4). In two cases a single base pair mutations was found (Table 1), whereas in two additional cases two mutations were identified in the tumors. Codon 816 mutations, which have been found previously in germ cell tumors and mastocytosis (Nagata et al, 1995; Tian et al, 1999), were ...
... unique (Figs. 3 and 4). In two cases a single base pair mutations was found (Table 1), whereas in two additional cases two mutations were identified in the tumors. Codon 816 mutations, which have been found previously in germ cell tumors and mastocytosis (Nagata et al, 1995; Tian et al, 1999), were ...
Sugar signals and the control of plant growth and
... UDP-glucose (UDPG) to the growth signalling molecule T6P. Metabolism of T6P by trehalose-6-phosphate phosphatase (TPP) yields trehalose, which is hydrolysed to glucose by Trehalase1. T6P levels in Arabidopsis are correlated to those of sucrose, but cellular T6P levels are in the low micromolar range ...
... UDP-glucose (UDPG) to the growth signalling molecule T6P. Metabolism of T6P by trehalose-6-phosphate phosphatase (TPP) yields trehalose, which is hydrolysed to glucose by Trehalase1. T6P levels in Arabidopsis are correlated to those of sucrose, but cellular T6P levels are in the low micromolar range ...
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... KH domains and RGG box of FMRP have been found to mediate FMRP-RNA interactions both in vitro and in vivo [2,16,17,41,43]. Both motifs contribute to the role of FMRP as a suppressor of target messenger RNA (mRNA) translation via binding of noncoding RNA structures, including G-quartets and “kissing ...
... KH domains and RGG box of FMRP have been found to mediate FMRP-RNA interactions both in vitro and in vivo [2,16,17,41,43]. Both motifs contribute to the role of FMRP as a suppressor of target messenger RNA (mRNA) translation via binding of noncoding RNA structures, including G-quartets and “kissing ...
Origin of Cancer: An Information, Energy, and Matter Disease
... thousands of mutations in cancer-relevant genes, including cancer-driver genes, in normal eyelid epidermis that rarely develop cancers (Martincorena et al., 2015). This finding stands in contrast to the mutation hypothesis because, if mutations really are the exclusive cause of cancer, then how can ...
... thousands of mutations in cancer-relevant genes, including cancer-driver genes, in normal eyelid epidermis that rarely develop cancers (Martincorena et al., 2015). This finding stands in contrast to the mutation hypothesis because, if mutations really are the exclusive cause of cancer, then how can ...
The regulation of Krox-20 expression reveals important steps in the
... (Topilko et al., 1994 and data not shown), these mice provide a sensitive and simple system for studying Krox-20 regulation. In heterozygous embryos, β-galactosidase is first detected in the PNS around 10.5 days post coitum (dpc) in boundary cap cells (Wilkinson et al., 1989; Schneider-Maunoury et a ...
... (Topilko et al., 1994 and data not shown), these mice provide a sensitive and simple system for studying Krox-20 regulation. In heterozygous embryos, β-galactosidase is first detected in the PNS around 10.5 days post coitum (dpc) in boundary cap cells (Wilkinson et al., 1989; Schneider-Maunoury et a ...
Chapter 7 Cell Structure and Function Section: 7-1 Life
... End Show Copyright Pearson Prentice Hall ...
... End Show Copyright Pearson Prentice Hall ...
Secretion of type II extracellular cell wall degrading
... The changes of α, β - galactosidase and β - glucosidase were investigated throughout the growth of the P. agglomerans. The maximum α - galactosidase activity of P. agglomerans was recorded in D with two peaks at 72 h and 144 h respectively. In media C activity was started from 96 h and remained up t ...
... The changes of α, β - galactosidase and β - glucosidase were investigated throughout the growth of the P. agglomerans. The maximum α - galactosidase activity of P. agglomerans was recorded in D with two peaks at 72 h and 144 h respectively. In media C activity was started from 96 h and remained up t ...
Cardiac Cell Lineages that Form the Heart
... Retrospective clonal analysis in the mouse embryo (see Buckingham and Meilhac 2011) indicated that two major lineages contribute to the myocardium of the heart. The first lineage contributes left ventricular myocardium, whereas the second lineage is the source of outflow tract and most right ventric ...
... Retrospective clonal analysis in the mouse embryo (see Buckingham and Meilhac 2011) indicated that two major lineages contribute to the myocardium of the heart. The first lineage contributes left ventricular myocardium, whereas the second lineage is the source of outflow tract and most right ventric ...
Retinal pigment epithelial cells phagocytosis of T lymphocytes
... Confluent hRPE cells were co-cultured with red labelled T lymphocytes for 2, 6, or 24 hours before being trypsinised and analysed by flow cytometry. We have defined, within the forward scatter/side scatter (FSC/SSC) dot plot, a subregion G1 corresponding to the large majority of RPE cells, where nea ...
... Confluent hRPE cells were co-cultured with red labelled T lymphocytes for 2, 6, or 24 hours before being trypsinised and analysed by flow cytometry. We have defined, within the forward scatter/side scatter (FSC/SSC) dot plot, a subregion G1 corresponding to the large majority of RPE cells, where nea ...
Compartments Target the Antigen to Distinct Intracellular B Cell
... neurotoxin (TeNT) enhances their presentation to specific T cells (28). One part of this effect can be explained by increased internalization of C3b-TeNT complexes mediated by complement receptors (CR) (29). Moreover, C3b induces some modifications of processing of the Ag linked (30), resulting in i ...
... neurotoxin (TeNT) enhances their presentation to specific T cells (28). One part of this effect can be explained by increased internalization of C3b-TeNT complexes mediated by complement receptors (CR) (29). Moreover, C3b induces some modifications of processing of the Ag linked (30), resulting in i ...
Novel Potentiators Augment Efficacy of Translational
... 10,000 nM) of GP-2, VX-770 in FRT G542X cells and (B) FRT W1282X cells. Scatter plots representing maximum efficacy of potentiators at 10 μM in (C) FRT G542X cells and (D) FRT W1282X cells. (E) Enhanced CFTR activity in the presence of 10 μM potentiators in FRT R1162X cells. In all the three PTC mut ...
... 10,000 nM) of GP-2, VX-770 in FRT G542X cells and (B) FRT W1282X cells. Scatter plots representing maximum efficacy of potentiators at 10 μM in (C) FRT G542X cells and (D) FRT W1282X cells. (E) Enhanced CFTR activity in the presence of 10 μM potentiators in FRT R1162X cells. In all the three PTC mut ...
THE PLAY ABOUT A PLANT CELL, AN ANIMAL CELL, AND A
... Mitochondria: (Move very quickly passing out energy drinks to the organelles) Mitochondira #1: (Bumps into Oxy while passing out energy) Oxy: Umph….What was that? Cell Membrane: Those were the mitochondria. Oxy: The way they’re moving around here, they must have lots of energy. Mitochondria: (speak ...
... Mitochondria: (Move very quickly passing out energy drinks to the organelles) Mitochondira #1: (Bumps into Oxy while passing out energy) Oxy: Umph….What was that? Cell Membrane: Those were the mitochondria. Oxy: The way they’re moving around here, they must have lots of energy. Mitochondria: (speak ...
Measuring the mechanical properties of plant cells by combining
... Several models have been proposed that describe indentation experiments at different scales. A mathematical model that is often used to interpret data from AFM experiments is the Hertz model (Lin et al., 2007). This assumes that indentations are small enough to only probe elastic properties of the ...
... Several models have been proposed that describe indentation experiments at different scales. A mathematical model that is often used to interpret data from AFM experiments is the Hertz model (Lin et al., 2007). This assumes that indentations are small enough to only probe elastic properties of the ...
Immunity Cells Programmed by Mediators of Type 1 Nanotube
... The Journal of Immunology recipient DC1 or DC2 were harvested, washed five times, and cocultured for 20 h in the presence or absence of rhCD40L. Where stated, a 0.45-mm transwell system (Corning Costar, Tewksbury, MA) was used to separate cocultured DC. Bead transfer to recipient DC was assessed by ...
... The Journal of Immunology recipient DC1 or DC2 were harvested, washed five times, and cocultured for 20 h in the presence or absence of rhCD40L. Where stated, a 0.45-mm transwell system (Corning Costar, Tewksbury, MA) was used to separate cocultured DC. Bead transfer to recipient DC was assessed by ...
Mutations affecting development of zebrafish digestive organs
... portal blood. The intrahepatic bile ducts arise from bile cannaliculi and are scattered throughout the liver parenchyma. As in other vertebrates, a gall bladder is present for the storage of bile. The adult zebrafish pancreas (Fig. 1C) is a less coherent structure than that of the mammal in that sev ...
... portal blood. The intrahepatic bile ducts arise from bile cannaliculi and are scattered throughout the liver parenchyma. As in other vertebrates, a gall bladder is present for the storage of bile. The adult zebrafish pancreas (Fig. 1C) is a less coherent structure than that of the mammal in that sev ...
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... higher temperature. When an animal fails to respond to the higher temperature by regeneration at the graft-host border the positional communication channel may be considered to be re-established. Gap junctions can be recognized in thin sections of normal tissue by the characteristic reduction in mem ...
... higher temperature. When an animal fails to respond to the higher temperature by regeneration at the graft-host border the positional communication channel may be considered to be re-established. Gap junctions can be recognized in thin sections of normal tissue by the characteristic reduction in mem ...
High-throughput functional genomics using
... of the reagent type, the resultant siRNAs are then loaded into the RNA-induced silencing complex (RISC), which is guided to the target mRNA molecule by the siRNA to initiate mRNA degradation or translational inhibition23. Catalytically inactive Cas9 for transcriptional modu‑ lation. In addition to g ...
... of the reagent type, the resultant siRNAs are then loaded into the RNA-induced silencing complex (RISC), which is guided to the target mRNA molecule by the siRNA to initiate mRNA degradation or translational inhibition23. Catalytically inactive Cas9 for transcriptional modu‑ lation. In addition to g ...
- University of East Anglia
... observed following ectopic expression of Klhl31 in the neural tube, suggesting an indirect effect on the adjacent somite. Gain-of-function experiments were complemented by morpholino (MO) mediated knock-down (KD) of Klhl31 in the ...
... observed following ectopic expression of Klhl31 in the neural tube, suggesting an indirect effect on the adjacent somite. Gain-of-function experiments were complemented by morpholino (MO) mediated knock-down (KD) of Klhl31 in the ...
The nonhomologous end joining factor Artemis suppresses multi
... Nonhomologous end joining (NHEJ) is a critical DNA repair pathway, with proposed tumor suppression functions in many tissues. Mutations in the NHEJ factor ARTEMIS cause radiation-sensitive severe combined immunodeficiency in humans and may increase susceptibility to lymphoma in some settings. We now ...
... Nonhomologous end joining (NHEJ) is a critical DNA repair pathway, with proposed tumor suppression functions in many tissues. Mutations in the NHEJ factor ARTEMIS cause radiation-sensitive severe combined immunodeficiency in humans and may increase susceptibility to lymphoma in some settings. We now ...
Life under pressure: hydrostatic pressure in cell growth and function
... Activation of ion fluxes during cell volume perturbation and recovery There is a dynamic balance between the osmotic potential across the plasma membrane and cell volume, which is dependent on intracellular metabolic flux and extracellular conditions. In neuronal cells undergoing intense activation, ...
... Activation of ion fluxes during cell volume perturbation and recovery There is a dynamic balance between the osmotic potential across the plasma membrane and cell volume, which is dependent on intracellular metabolic flux and extracellular conditions. In neuronal cells undergoing intense activation, ...