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Spatial and temporal distribution of the adherens
Spatial and temporal distribution of the adherens

... molecule) is tightly associated with the plasma membrane of intercellular adherens-type junctions of a variety of cell types, such as chick cardiac myocytes and lens cells (Geiger et al. 1985a; Volk & Geiger, 1986a). A-CAM is apparently an integral membrane glycoprotein with an extracellular domain ...
Signal Requirements for the Generation of CD4+ and CD8+ T
Signal Requirements for the Generation of CD4+ and CD8+ T

... effector cells and whether EC alloantigens were actually involved in the generation of the response, multiple genetically unrelated EC lines were used as targets in the final cytotoxicity assay, in addition to the NKsensitive line, K562. The results (Figures 2a and 2b) demonstrate that the two most ...
GlischSpr11
GlischSpr11

... Staphylococcus aureus (MRSA) in Ecuador. Nasal swabs collected at a hospital in the Loja province were brought back to the University of Wisconsin-Eau Claire to be analyzed for MRSA. Nasal swabs were identified through a series of experiments shown in figure A. In order to confirm that these samples ...
Endomucin, a CD34-like sialomucin, marks hematopoietic stem cells
Endomucin, a CD34-like sialomucin, marks hematopoietic stem cells

... and identified 46 genes. Of these, 36 were known murine genes, 4 were putative murine homologues of human genes, and 6 were unknown murine genes (Table S1, available at http://www.jem.org/cgi/content/full/jem.20051325/DC1). RT-PCR analysis of selected genes revealed that several genes were preferent ...
Identification of a Mid-anaphase Checkpoint in
Identification of a Mid-anaphase Checkpoint in

... Studies in populations of budding yeast, after activation of a facultative, dicentric chromosome are also indicative of a mid-anaphase cell cycle checkpoint which can delay progression through mitosis (Neff and Burke, 1992; Brock and Bloom, 1994). These dicentric chromosomes contain two centromeres, ...
NAC Transcription Factor SPEEDY HYPONASTIC
NAC Transcription Factor SPEEDY HYPONASTIC

... domains, we assumed that these proteins may share similar DNA binding specificity. Previously, two high-affinity binding sites of Ta-NAC69 (site I, rrwkmCGTrnnnnnyACGtmayy; site II, rswvktynnnnnnnnyACGwcwct) were identified through in vitro binding site selection, followed by extensive mutation analysi ...
Role of cryo-ET in membrane bioenergetics research
Role of cryo-ET in membrane bioenergetics research

... Smaller proteins or proteins of unknown or uncharacteristic shape have to be identified by labelling. Electron-dense tags such as colloidal gold or quantum dots conjugated to primary or secondary antibodies are easily visible in cryotomograms. Protein densities within a ∼23 nm radius of these tags a ...
File
File

... The Inner Life of the Cell Video of the Cell in Action ...
Genetic transformation of HeLa cells by Agrobacterium - igem
Genetic transformation of HeLa cells by Agrobacterium - igem

... border are more consistent and occur close to or within the border sequence, whereas the left border junctions can be spread over much longer segments, often deleting the border itself (reviewed in refs. 32–34). Similar preservation of the right border region relative to the left border region was o ...
Single Molecule Detection in Life Science
Single Molecule Detection in Life Science

... detection (SMD) techniques has opened up a new era of life science. The dynamic properties of biomolecules and the unique operations of molecular machines, which were hidden in averaged ensemble measurements, have been unveiled. The SMD techniques have rapidly been expanding to include a wide range ...
Cell surface mechanics and the control of cell shape, tissue patterns
Cell surface mechanics and the control of cell shape, tissue patterns

... pathways that spatially and temporally control cell behaviour. However, how this information is processed to control cell shape and cell dynamics is an open area of investigation. The framework that emerges from diverse disciplines such as cell biology, physics and developmental biology points to ad ...
Converting nonsense codons into sense codons by targeted
Converting nonsense codons into sense codons by targeted

... with the results of our in vitro analysis (Fig. 2B), only CUP1 mRNA isolated from cells expressing snR81-1C produced a spot corresponding to Y (Fig. 2C, compare panel c with panel d). Quantification indicated that approximately 5% of the cup1-PTC transcript was pseudouridylated. Thus, our in vivo ps ...
Membrane Transport - Austin Publishing Group
Membrane Transport - Austin Publishing Group

... pumps, just to list a few. Further, transport proteins may be classified by the substance that they transport, whether they are always open or gated (open only when stimulated), and most importantly, whether a source of free energy is required for operation. ...
Amphibian aquaporins and adaptation to terrestrial environments: A
Amphibian aquaporins and adaptation to terrestrial environments: A

... et al., 2005). AQP2 mutations and disruption of AQP2 gene cause nephrogenic diabetes insipidus, a disease characterized by a massive loss of water through the kidney (Deen et al., 1994; Yang et al., 2001). Other renal AQPs are also shown to play critical roles in water reabsorption and urine concent ...
Liver Physiology. ppt
Liver Physiology. ppt

... ...
Regulation of developmental timing and germ cell proliferation in C
Regulation of developmental timing and germ cell proliferation in C

... stability,13,14 making it an attractive system with which to study the p53 regulatory network. The CEP-1 DNA-binding domain is similar to that of human p5315 and CEP-1 binds to the p53 DNA consensus site.14,15 DNA damage in C. elegans is sensed by the HUS-1 and MRT-2 checkpoint proteins, which are r ...
Role of bacteriophage SPP1 tail spike protein gp21 on host cell
Role of bacteriophage SPP1 tail spike protein gp21 on host cell

... The virus particle of the vast majority of studied bacteriophages is composed of an icosahedral capsid attached to a tail structure, defining the order Caudovirales. The capsid, or phage head, shields a double-stranded DNA molecule while the tail is responsible for specific adsorption/binding to hos ...
Design and application of stimulus
Design and application of stimulus

... Several peptide-based systems have rationally been engineered, both de novo and as modifications to existing proteins, to respond in interesting ways to various stimuli (Table I). Many of these systems are tunable via changes in temperature and/or pH. For example, an early study described the de nov ...
Ultrastructural changes in the root tip and leaf cells of Lens culinaris
Ultrastructural changes in the root tip and leaf cells of Lens culinaris

... was increased as a result of exposure to fluazifopbutyl (Xiao & Hiroshi, 2002). At half to quadruple the strength of recommended field application concentrations, FPB adversely affected the development of both native and introduced species, grasses and nongrasses (Rokich et al., 2009). Fayez and Kri ...
Bacillus globigii cell size is influenced by
Bacillus globigii cell size is influenced by

... 15 % smaller than the untreated cells and cells treated with the original EDF with the sequence NNWNN. The diameter of both treated and untreated cells remained comparable. Typical examples of control cells and treated cells are shown in Fig. 2b. Next we assessed whether this effect was irreversible ...
Evolution of Gamete Recognition Proteins
Evolution of Gamete Recognition Proteins

... Sexual reproduction, defined here as the fusion of two haploid cells during fertilization to form a diploid zygote, occurs in almost all eukaryotes. Fertilization is the bridge between generations. Although studied for more than a century, it remains one of the least understood fundamental biologica ...
11206 bajek.vp
11206 bajek.vp

... muscle tissue. Following injury, overloading, and exacerbated stretching, these cells become activated. During this process, specific myogenic transcriptional factors are up regulated in myogenesis. These transcriptional factors are myf5, MyoD, myogenin and MRF4. Inspite the unequal results dealing ...
Clove
Clove

... Q 1. Did you ever see these? What are they ? Ans: Yes – they are cloves. Q 2. Did you ever used them in your house? Ans : yes. FIG 88.1 ...
INTERLEUKIN 6 DECREASES CELL
INTERLEUKIN 6 DECREASES CELL

... carcinoma cells were obtained from the American Type Culture Collection, Rockville, MD. The T-47 line was established from the pleural effusion obtained from a female patient with an infiltrating ductal carcinoma ofthe breast (30). The differentiated epithelial subline T47D (30) contains cytoplasmic ...
Gastrulation in the sea anemone Nematostella
Gastrulation in the sea anemone Nematostella

... Abstract The sea anemone Nematostella vectensis has recently been established as a new model system for the understanding of the evolution of developmental processes. In particular, the evolutionary origin of gastrulation and its molecular regulation are the subject of intense investigation. However ...
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