Multiscale View of Cytoskeletal Mechanoregulation of Cell and
... Beausang et al. 2008) renders them key players in the generation and maintenance of cell polarity. The myosin superfamily is large and versatile; however, it can be divided into two groups: conventional and unconventional myosins. Conventional myosin, also known as myosin II, assembles into bipolar ...
... Beausang et al. 2008) renders them key players in the generation and maintenance of cell polarity. The myosin superfamily is large and versatile; however, it can be divided into two groups: conventional and unconventional myosins. Conventional myosin, also known as myosin II, assembles into bipolar ...
Atomic Force Microscopy in Cancer Cell Research
... surface near its contact with AFM tip. If the surface were ideally flat, then it would be irrelevant at what point to measure forces. In the case of biological cells, knowledge of geometry is important because cells are not necessarily flat. Therefore, one will need some kind of automatic collection o ...
... surface near its contact with AFM tip. If the surface were ideally flat, then it would be irrelevant at what point to measure forces. In the case of biological cells, knowledge of geometry is important because cells are not necessarily flat. Therefore, one will need some kind of automatic collection o ...
Multiple Mechanisms of Resistance to Polyglutamatable and
... (Allegra et al., 1987; Borst and Ouelette, 1995). Because lipophilic antifolates can enter cells and tissues by passive or facilitated diffusion, they have been exploited as anticancer agents to circumvent transport-related resistance to classic antifolate drugs such as methotrexate (MTX) (Bertino, ...
... (Allegra et al., 1987; Borst and Ouelette, 1995). Because lipophilic antifolates can enter cells and tissues by passive or facilitated diffusion, they have been exploited as anticancer agents to circumvent transport-related resistance to classic antifolate drugs such as methotrexate (MTX) (Bertino, ...
Weekly Lesson Plans Teacher: Alicia Penfield Class: AP Biology
... role of matter and energy in is an anabolic reaction, and this process. its overall purpose. Distinguish exergonic and Summarize each stage of endergonic reactions, and photosynthesis, including the give examples of each. light reaction and Calvin cycle, including beginning Describe how the st ...
... role of matter and energy in is an anabolic reaction, and this process. its overall purpose. Distinguish exergonic and Summarize each stage of endergonic reactions, and photosynthesis, including the give examples of each. light reaction and Calvin cycle, including beginning Describe how the st ...
Print
... Li, L. Zhong, and D. Bredesen, unpublished results). In any case, however, the majority of cellular proteins do not appear to be caspase substrates. The mammalian homolog (or at least one homolog) of ced-4 appears to be Apaf-1 (152). This protein, along with ATP, caspase-9 zymogen, and cytochrome c ...
... Li, L. Zhong, and D. Bredesen, unpublished results). In any case, however, the majority of cellular proteins do not appear to be caspase substrates. The mammalian homolog (or at least one homolog) of ced-4 appears to be Apaf-1 (152). This protein, along with ATP, caspase-9 zymogen, and cytochrome c ...
Transendothelial Migration Surface Determinants During Activated T
... staining, as previously described (2), with FITC- and/or PE-labeled mAb for the expression of various surface receptors, including CD69 (Leu23 PE), CD3 (Leu4 PE), and CD54 (Leu54 PE), all purchased from Becton Dickinson (Mountain View, CA); CD4 (Q4120 PE), purchased from Sigma; CD62E (1.2B6 FITC), p ...
... staining, as previously described (2), with FITC- and/or PE-labeled mAb for the expression of various surface receptors, including CD69 (Leu23 PE), CD3 (Leu4 PE), and CD54 (Leu54 PE), all purchased from Becton Dickinson (Mountain View, CA); CD4 (Q4120 PE), purchased from Sigma; CD62E (1.2B6 FITC), p ...
The structural biology of the amyloid precursor protein
... ubiquitously expressed isoforms of APP, APP751 and APP770 carry the additional KPI and the OX2-segment in-between the AcD and E2. On one hand this overall domain architecture is in line with many experimental observations ...
... ubiquitously expressed isoforms of APP, APP751 and APP770 carry the additional KPI and the OX2-segment in-between the AcD and E2. On one hand this overall domain architecture is in line with many experimental observations ...
Oxidation of Hg(0) - Department of Marine and Coastal Sciences
... elements such as Fe(II), As(III), and U(IV) (Osborne and Ehrlich, 1976; Widdel et al., 1993; Finneran et al., 2002). Because of the relatively high redox potential of the Hg(II)/Hg(0) couple (Eo = +0.8 V), Hg(0) is expected to be the stable oxidation state of mercury in suboxic redox zones. In these ...
... elements such as Fe(II), As(III), and U(IV) (Osborne and Ehrlich, 1976; Widdel et al., 1993; Finneran et al., 2002). Because of the relatively high redox potential of the Hg(II)/Hg(0) couple (Eo = +0.8 V), Hg(0) is expected to be the stable oxidation state of mercury in suboxic redox zones. In these ...
POM-POM2/CELLULOSE SYNTHASE
... This construct did not rescue the pom2 mutants, and the fluorescent pattern was diffuse and distinct from the cortical foci seen using the full-length POM/CSI1:CFP construct (see Supplemental Figure 5C online), suggesting that truncations of the POM2/CSI1 protein render it nonfunctional and that the ...
... This construct did not rescue the pom2 mutants, and the fluorescent pattern was diffuse and distinct from the cortical foci seen using the full-length POM/CSI1:CFP construct (see Supplemental Figure 5C online), suggesting that truncations of the POM2/CSI1 protein render it nonfunctional and that the ...
View PDF - G3: Genes | Genomes | Genetics
... 1992; Clancy et al. 2002). Previous work indicated that Ime4 promotes the accumulation of IME1 transcripts, suggesting that there is positive regulation between the two genes during entry into sporulation (Shah and Clancy 1992). Yeast cells undergo gametogenesis relatively asynchronously, making it ...
... 1992; Clancy et al. 2002). Previous work indicated that Ime4 promotes the accumulation of IME1 transcripts, suggesting that there is positive regulation between the two genes during entry into sporulation (Shah and Clancy 1992). Yeast cells undergo gametogenesis relatively asynchronously, making it ...
The many ways to age for a single yeast cell
... to the mitochondrial pathway of apoptosis, causes an increase of replicative lifespan, indicating that distinct components of the (mitochondrial) apoptotic machinery are indeed involved in the determination of replicative lifespan. As these proteins have been described to have pro-apoptotic (Ybh3p, ...
... to the mitochondrial pathway of apoptosis, causes an increase of replicative lifespan, indicating that distinct components of the (mitochondrial) apoptotic machinery are indeed involved in the determination of replicative lifespan. As these proteins have been described to have pro-apoptotic (Ybh3p, ...
Galectin-3 Cleavage Alters Bone Remodeling: Different Outcomes in
... bone, and in giant cell tumor, which is a benign osteoclast producing tumor (27). In the tissue microarray of osteosarcoma, Gal-3–positive cells appeared to congregate near the matured osteoclasts (Supplementary Fig. S1B). Similarly, in the giant cell tumor tissue array, immunoreactions with Gal-3 a ...
... bone, and in giant cell tumor, which is a benign osteoclast producing tumor (27). In the tissue microarray of osteosarcoma, Gal-3–positive cells appeared to congregate near the matured osteoclasts (Supplementary Fig. S1B). Similarly, in the giant cell tumor tissue array, immunoreactions with Gal-3 a ...
Spontaneous zygogenesis in Escherichia coli, a form
... preventing any cross-feeding of sticky cells, bacteria picked up from the selected colony were suspended in 10 ml M9 and these suspensions were strongly vortexed for 30 s. Furthermore, samples were streaked onto the medium used in the initial selection. Two to three colonies per isolated clone were ...
... preventing any cross-feeding of sticky cells, bacteria picked up from the selected colony were suspended in 10 ml M9 and these suspensions were strongly vortexed for 30 s. Furthermore, samples were streaked onto the medium used in the initial selection. Two to three colonies per isolated clone were ...
Syringolin, a Novel Peptide Elicitor from Pseudomonas syringae pv
... observable aspect of this defense response is the increased abundance of a set of transcripts. The accumulation of one of these transcripts, Pir7b, was dependent on the function of the bacterial lemA gene, which encodes part of a twocomponent regulatory system. This suggested that the lemA regulator ...
... observable aspect of this defense response is the increased abundance of a set of transcripts. The accumulation of one of these transcripts, Pir7b, was dependent on the function of the bacterial lemA gene, which encodes part of a twocomponent regulatory system. This suggested that the lemA regulator ...
Journal of Bacteriology
... f Cell surface preparations were separated into two fractions by ultrafiltration, using a 30- and a 5-kDa membrane. Equal amounts corresponding to a cell surface preparation derived from 10 ml of culture were used in the ...
... f Cell surface preparations were separated into two fractions by ultrafiltration, using a 30- and a 5-kDa membrane. Equal amounts corresponding to a cell surface preparation derived from 10 ml of culture were used in the ...
Review The Role of Laminin in Embryonic Cell Polarization and
... Genetic analyses have revealed that members of the laminin glycoprotein family are required for basement membrane assembly and cell polarization, with subsequent effects on cell survival and tissue organization during metazoan embryogenesis. These functions depend upon the cooperation between lamini ...
... Genetic analyses have revealed that members of the laminin glycoprotein family are required for basement membrane assembly and cell polarization, with subsequent effects on cell survival and tissue organization during metazoan embryogenesis. These functions depend upon the cooperation between lamini ...
SCIENTIFIC ACHIEVEMENTS My laboratory was the first to identify
... We were the first to identify the molecular mechanism of the Charcot Marie Tooth 4J neuropathy, caused by a compound heterozygous null and point Sac3 (FIG4)I41T mutation. This mechanism involves unusually fast degradation of Sac3I41T, because, contrary to Sac3WT, mutant Sac3I41T is unable to be prot ...
... We were the first to identify the molecular mechanism of the Charcot Marie Tooth 4J neuropathy, caused by a compound heterozygous null and point Sac3 (FIG4)I41T mutation. This mechanism involves unusually fast degradation of Sac3I41T, because, contrary to Sac3WT, mutant Sac3I41T is unable to be prot ...
Homologies of process and modular elements of embryonic
... functional criteria from homology definitions or assessments. It is not at all easy to define a process independent of its function. The second is that although hypotheses of homology must specify a particular level of biological organization (Striedter and Northcutt, ’91; Bolker and Raff, ’96; Abou ...
... functional criteria from homology definitions or assessments. It is not at all easy to define a process independent of its function. The second is that although hypotheses of homology must specify a particular level of biological organization (Striedter and Northcutt, ’91; Bolker and Raff, ’96; Abou ...
Cell-A-Brate - 6.LS.1 - Columbus City Schools
... these you should point out that in both there is a progression of size but in the levels of organization of living things each level is a part of the level above it working from smallest to largest. The final product can be made with random cells, etc., but higher level students should be encouraged ...
... these you should point out that in both there is a progression of size but in the levels of organization of living things each level is a part of the level above it working from smallest to largest. The final product can be made with random cells, etc., but higher level students should be encouraged ...
extracellular matrix remodeling and integrin
... contraction leads to basement membrane remodeling, which may facilitate localized proliferation and subsequent bud elongation. (b) Integrin signaling during somite morphogenesis. Somite formation depends upon fibronectin assembly at the intersomitic boundary. Activation of integrin a5b1 induces fibr ...
... contraction leads to basement membrane remodeling, which may facilitate localized proliferation and subsequent bud elongation. (b) Integrin signaling during somite morphogenesis. Somite formation depends upon fibronectin assembly at the intersomitic boundary. Activation of integrin a5b1 induces fibr ...
Two-photon laser scanning microscopy imaging of intact spinal cord and
... lack CXCR6 expression, but instead express only cytoplasmic GFP (Geissmann et al., 2005). We utilized this mouse strain on the B6 background to study the dynamics of GFP+ cells in the living mouse spinal cord and brain during EAE. CXCR6+ cells migrated rapidly in the spinal cord with a rostrocaudal ...
... lack CXCR6 expression, but instead express only cytoplasmic GFP (Geissmann et al., 2005). We utilized this mouse strain on the B6 background to study the dynamics of GFP+ cells in the living mouse spinal cord and brain during EAE. CXCR6+ cells migrated rapidly in the spinal cord with a rostrocaudal ...
The Role of Two-Component Signal Transduction Systems in
... by two-component signaling pathways that couple changes in environmental conditions to the production of c-di-GMP by phosphorylating response regulators with GGDEF output domains. For instance, in Pseudomonas aeruginosa, a chemotaxis-like system drives the phosphorylation of the response regulator W ...
... by two-component signaling pathways that couple changes in environmental conditions to the production of c-di-GMP by phosphorylating response regulators with GGDEF output domains. For instance, in Pseudomonas aeruginosa, a chemotaxis-like system drives the phosphorylation of the response regulator W ...
DETECÇÃO DE S100-β E REAÇÃO DE
... autocrine and paracrine action and is considered an intercellular signaling. S100-β may be implicated in the pathophysiology of brain disorders, through increased release by astrocytes consequent to their activation and/ or death (Adami et al., 2001, Rothermundt et al., 2003, Donato et al., 2003, Ma ...
... autocrine and paracrine action and is considered an intercellular signaling. S100-β may be implicated in the pathophysiology of brain disorders, through increased release by astrocytes consequent to their activation and/ or death (Adami et al., 2001, Rothermundt et al., 2003, Donato et al., 2003, Ma ...
Genetic studies in NZB mice. V. Recombinant inbred lines
... recombinant inbred (RI) lines, to further analyze the inheritance of autoimmunity (17-20). R I lines were obtained by mating an NZB mouse with a non-autoimmune mouse. The F2 mice were separately mated to initiate the R I lines and then brothersister mated at each successive generation. Each R I line ...
... recombinant inbred (RI) lines, to further analyze the inheritance of autoimmunity (17-20). R I lines were obtained by mating an NZB mouse with a non-autoimmune mouse. The F2 mice were separately mated to initiate the R I lines and then brothersister mated at each successive generation. Each R I line ...
Mechanisms of Hormone Action: Steroid Receptors
... influenced by the protein kinase pathways. Example: Activation of PKC decreases estrogen receptor mRNA stability, resulting in decreased synthesis. ...
... influenced by the protein kinase pathways. Example: Activation of PKC decreases estrogen receptor mRNA stability, resulting in decreased synthesis. ...