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Localization of the Microtubule End Binding Protein
Localization of the Microtubule End Binding Protein

... Otegui and Staehelin, 2000). This, the phragmoplast, then grows out centrifugally, opening up as a ring that deposits the cell plate in its center. Microtubule hook decoration studies have shown that phragmoplast microtubules share the same polarity as those of the spindle, with the plus ends overla ...
"Molecular Motors in Plant Cells". In: Molecular Motors
"Molecular Motors in Plant Cells". In: Molecular Motors

... group of KLPs pre-dates the divergence of plants and animals from a common ancestor which is believed to have occurred about 1.5 billion years ago (Wang et al., 1999). If this was the case, there must have been insertion of some domains such as MyTH4 and talin-like regions in the tail of KCBP (or de ...
Cytoskeletal Basis of Organelle Trafficking in the
Cytoskeletal Basis of Organelle Trafficking in the

... the pollen tube are known in general terms, but many points remain to be clarified. For example, except for the movements of specific organelles observable through the optical microscope, little information exists on other types of intracytoplasmic movement in the pollen tube and on their relationsh ...
Histochemical Polymorphism of Keratin Pearls in Squamous Cell
Histochemical Polymorphism of Keratin Pearls in Squamous Cell

... In conventional histological hematoxylin and eosin staining the third type of keratin pearls is revealed, which are characterized with weak eosinophilic staining of cells’ cytoplasm or contained foam cytoplasm. Nuclei of such cells, located on the periphery, are large and ovoid, hyperchromatic with ...
life in a drop of water
life in a drop of water

... variety of fascinating pond organisms. The narrator then states that these organisms can be found in every lake, pond, stream and puddle. Mostly microscopic in size, hundreds, even thousands, can be found in a single drop of water. Students are then featured at a pond collecting pond water to study ...
Two Light-dependent Conductances in Lima Rhabdomeric
Two Light-dependent Conductances in Lima Rhabdomeric

... The amplitude and the time course of the photocurrent were examined as a function of the intensity of stimulating light. Fig. 2, A and B, shows typical light intensity series for two different cells. At low light levels the response to a 100°ms flash is quite slow, with latency on the order of 300 m ...
Actin behavior in bulk cytoplasm is cell cycle regulated in early
Actin behavior in bulk cytoplasm is cell cycle regulated in early

... ionophore, before extract preparation; and (ii) 400 M Ca2+ was added to M-phase extracts to breakdown CSF, followed by incubation for 30 minutes at 20°C to allow the cell cycle to progress. The added Ca2+ is rapidly sequestered into endoplasmic reticulum (ER) in the extract, so this treatment resem ...
Alamethicin permeabilizes the plasma membrane and mitochondria
Alamethicin permeabilizes the plasma membrane and mitochondria

... To investigate the effect of AlaM on plant cells, growing cells were harvested and their oxygen consumption registered during incubation with different concentrations of AlaM (Figure 1a). With increasing concentrations of AlaM, two effects were seen. A transient increase in oxygen consumption occurr ...
Hormonal control of cell division and elongation
Hormonal control of cell division and elongation

... in stem cell specification in roots (Fig. 1; Sabatini et al., 1999). RETINOBLASTOMA-RELATED protein (RBR) is the plant orthologue of mammalian retinoblastoma protein, and inhibits G1 to S transition by repressing E2F transcription factors (Xie et al., 1996; Nakagami et al., 1999). Reduction in RBR e ...
Profibrillin conversion by proprotein convertases
Profibrillin conversion by proprotein convertases

... Fibroblasts heterozygous for this mutation converted only onehalf of secreted profibrillin to fibrillin. In order to further characterize the cleavage site and to confirm that the furin/PACE family is responsible for processing profibrillin, we designed a construct encoding a mini-profibrillin prote ...
The chromosome partitioning protein, ParB, is required for
The chromosome partitioning protein, ParB, is required for

Physcomitrella Cyclin-Dependent Kinase A Links
Physcomitrella Cyclin-Dependent Kinase A Links

Selection and the Origin of Cells
Selection and the Origin of Cells

... suppose that clay crystals subjected to rounds of crystallization and fragmentation could evolve by selection, in the last fifty years, no plausible model has been proposed to explain how clay crystals could transition into growing, dividing protocells. In the third category, I would include classic ...
Atomic Force Microscopy in Cancer Cell Research
Atomic Force Microscopy in Cancer Cell Research

... surface near its contact with AFM tip. If the surface were ideally flat, then it would be irrelevant at what point to measure forces. In the case of biological cells, knowledge of geometry is important because cells are not necessarily flat. Therefore, one will need some kind of automatic collection o ...
Fukuda, Annu. Rev. Plant Physiol. Plant Mol. Biol
Fukuda, Annu. Rev. Plant Physiol. Plant Mol. Biol

... flow that starts initially by diffusion induces the formation of a polar auxin transport cell system, which in turn promotes auxin transport and leads to canalization of auxin flow along a narrow file of cells. This continuous polar transport of auxin through cells finally results in the differentia ...
Boron in plants: deficiency and toxicity
Boron in plants: deficiency and toxicity

... to deficiencies in plants that grow there (e. g., many regions in Japan, China, USA, and Brazil). On the contrary, under low rainfall conditions, B can not be sufficiently leached and therefore may accumulate to levels that become toxic to plant growth (Reid 2007b). This is very often in arid and se ...
Isolation and purification of cell wall polysaccharide of Bacillus
Isolation and purification of cell wall polysaccharide of Bacillus

... using gas liquid chromatography (GLC) and 13CN M R spectroscopy. Samples for GLC were prepared by suspending 1 mg of polysaccharide in 250 pA 2M trifluoroacetic acid and heated at 120°C for 1 h. The samples were cooled and acid removed under a flow of air and by co-distillation with isopropyl alcoho ...
and ______ are
and ______ are

... Images from: http://www.imac.auckland.ac.nz/vaccines/vacc_graph.htm ...
the fine-tuning of the endomembrane system
the fine-tuning of the endomembrane system

Cytometry and Plant Sciences: a Personal Retrospective David W. Galbraith
Cytometry and Plant Sciences: a Personal Retrospective David W. Galbraith

... remain topologically continuous with one another. Given that the entire mature form of a plant is achieved through regulated cell division and cell expansion, all cell walls can be viewed as a single continuum, termed the apoplast. The concept of flow cytometry and cell sorting arose in the late 1960 ...
Domain organization of human cleavage factor Im 1 Distinct
Domain organization of human cleavage factor Im 1 Distinct

... Eukaryotic messenger RNA precursors (pre-mRNAs) are synthesized and processed in the nucleus prior to their export to the cytoplasm, where they serve as templates for protein synthesis. Transcription is coupled spatially and temporally to capping of the premRNA at the 5’!end, splicing and 3’!end for ...
Plasma Lipoproteins
Plasma Lipoproteins

... the proton-pumping activity of endosomal ATPase), which allows separation of the LDL from its receptor. The receptors then migrate to one side of the endosome, whereas the LDLs stay free within the lumen of the vesicle. [Note: This structure is called CURL Compartment for Uncoupling for Receptor and ...
p23 and organization of the Golgi apparatus
p23 and organization of the Golgi apparatus

Understanding Activity in Electrically Coupled Networks Using PRCs
Understanding Activity in Electrically Coupled Networks Using PRCs

... Phase differences '  with G.'  / D 0 are phase-locked states. A phase-locked state is stable if G 0 .'  / < 0 and unstable if G 0 .'  / > 0. Note that the coupling strength ggap simply scales the G-function. It does not affect the existence or stability of the phase-locked states; it only affect ...
Intracellular Triggering of Fas Aggregation and Recruitment of
Intracellular Triggering of Fas Aggregation and Recruitment of

... which ET-18-OCH3 is selectively incorporated into tumor cell membrane rafts promoting their clustering as well as translocation of Fas and downstream signaling molecules into clustered rafts in a SMase-independent manner. ET18-OCH3–mediated apoptosis was dependent on intracellular activation of Fas, ...
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Endomembrane system

The endomembrane system is composed of the different membranes that are suspended in the cytoplasm within a eukaryotic cell. These membranes divide the cell into functional and structural compartments, or organelles. In eukaryotes the organelles of the endomembrane system include: the nuclear membrane, the endoplasmic reticulum, the Golgi apparatus, lysosomes, vesicles, endosomes and the cell membrane. The system is defined more accurately as the set of membranes that form a single functional and developmental unit, either being connected directly, or exchanging material through vesicle transport. Importantly, the endomembrane system does not include the membranes of mitochondria or chloroplasts.The nuclear membrane contains two lipid bilayers that encompass the contents of the nucleus. The endoplasmic reticulum (ER) is a synthesis and transport organelle that branches into the cytoplasm in plant and animal cells. The Golgi apparatus is a series of multiple compartments where molecules are packaged for delivery to other cell components or for secretion from the cell. Vacuoles, which are found in both plant and animal cells (though much bigger in plant cells), are responsible for maintaining the shape and structure of the cell as well as storing waste products. A vesicle is a relatively small, membrane-enclosed sac that stores or transports substances. The cell membrane, is a protective barrier that regulates what enters and leaves the cell. There is also an organelle known as the Spitzenkörper that is only found in fungi, and is connected with hyphal tip growth.In prokaryotes endomembranes are rare, although in many photosynthetic bacteria the plasma membrane is highly folded and most of the cell cytoplasm is filled with layers of light-gathering membrane. These light-gathering membranes may even form enclosed structures called chlorosomes in green sulfur bacteria.The organelles of the endomembrane system are related through direct contact or by the transfer of membrane segments as vesicles. Despite these relationships, the various membranes are not identical in structure and function. The thickness, molecular composition, and metabolic behavior of a membrane are not fixed, they may be modified several times during the membrane's life. One unifying characteristic the membranes share is a lipid bilayer, with proteins attached to either side or traversing them.
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