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SLP-76-Cbl-Grb2-Shc Interactions in Fc RI Signaling
SLP-76-Cbl-Grb2-Shc Interactions in Fc RI Signaling

... (Fig 1A). Marked tyrosine phosphorylation of a 76 kD protein was detected in the U937IF cells stimulated with anti-FcgRI (Fig 1A, lane 5). The 76 kD protein was not phosphorylated in resting cells (Fig 1A, lane 4). To confirm the identity of the 76 kD phosphoprotein, the membrane was reprobed with a ...
Intracellular marking of physiologically characterized cells in the
Intracellular marking of physiologically characterized cells in the

Pectin methylesterases: cell wall enzymes with important roles in
Pectin methylesterases: cell wall enzymes with important roles in

... detectable among proteins extracted from isolated cell walls, whereas they are abundant in proteins eluted directly from tissues10 or from infiltrated organs11. Because acidic isoforms are probably only weakly adsorbed onto the cell wall components, soluble-protein extraction procedures increase the ...
1 Breast Cancer Cell Line Development and Authentication
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... these cells are still proliferating in laboratories across the globe and used to increase our understanding of cellular mechanisms from cell signaling, to the implications of weighlessness/zero gravity on cellular aging, and everything in between. The implications of establishing this cell line have ...
Interactions of liposomes and lipid-based carrier systems with blood
Interactions of liposomes and lipid-based carrier systems with blood

... Liposomes and lipid-based drug delivery systems have been used extensively over the last decade to improve the pharmacological and therapeutic activity of a wide variety of drugs. More recently, this class of carrier systems has been used for the delivery of relatively large DNA and RNA-based drugs, ...
Processing of a Wheat Light-Harvesting Chlorophyll a/b Protein
Processing of a Wheat Light-Harvesting Chlorophyll a/b Protein

... were incubated with pea chloroplasts, which were then either treated or not treated with thermolysin before lysis. Tl~ermolysin has been shown to preferentially digest outer membrane proteins (10, 20) that have not entered the organelle. The membrane and soluble fractions of the chloroplast were sep ...
Phylogenomics demonstrates that breviate flagellates are related to
Phylogenomics demonstrates that breviate flagellates are related to

... that B. anathema is sister to the Amoebozoa with the ancyromonads sister to Opisthokonta þ apusomonads (OA) [21], but these results were again compromised by missing data for B. anathema. Most recently, a 30-protein phylogeny showed a BA clade with weak support that could not be conclusively placed ...
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Determination of the Correlation between Conductance and Architecture of Disordered ß-amyloid Channels
Determination of the Correlation between Conductance and Architecture of Disordered ß-amyloid Channels

... Introducing charge on the surrounding membrane, which replicates the effect of having charged lipids, results in the reduction of the conductance dynamic range for Scenario II (Table 3) (0.6810.36 nS) as compared to Scenario I (Table 2) (0.31-15.90 nS). The charged lipids are negatively charged, whi ...
Caldicellulosiruptor tāpirins bind to crystalline cellulose! ! 1 Discrete
Caldicellulosiruptor tāpirins bind to crystalline cellulose! ! 1 Discrete

... These extracellular proteins are used to attach to, modify, and hydrolyze the complex polysaccharides present in plant cell walls. Cellulolytic enzymes, often containing carbohydrate-binding modules (CBMs), are key to this process; however, these enzymes are not solely responsible for attachment. Fe ...
During Cytokinesis Light Chain, Tctex
During Cytokinesis Light Chain, Tctex

... (described below); these were the N-terminal domains of Fyn (amino acids 1– 85, a mutated version with lysines 7 and 9 mutated to alanines, and truncated versions with indicated amino acids), p55blk (Blk; amino acids 1–56), LynA (amino acids 1– 68), p53lyn (lynB; amino acids 1– 47), the cytoplasmic ...
Chapter 4 - A Tour of the Cell 08-09
Chapter 4 - A Tour of the Cell 08-09

... Copyright © 2007 Pearson Education Inc., publishing as Pearson Benjamin Cummings ...
Chen Tenwek 2015 Bullous Keratopathy
Chen Tenwek 2015 Bullous Keratopathy

...  Ablation of nerve plexus Lin P et al. Combined phototherapeutic keratectomy and therapeutic contact lens for recurrent erosions in bullous keratopathy. BJO 2001;85:908-11. Maini R et al. A comparison of different depth ablations in the treatment of painful bullous keratopathy with phototherapeutic ...
Roles of ion channels and transporters in guard cell signal
Roles of ion channels and transporters in guard cell signal

... and Arabidopsis show that blue light activates the H+-ATPase through a phosphorylation/ dephosphorylation-based mechanism [6,7]. When inactive, the C terminus of the H+-ATPase acts as an autoinhibitory domain. Upon blue light illumination, a type 1 protein phosphatase is activated [8] which, via an ...
Drosophila asymmetric division, polarity and cancer - e
Drosophila asymmetric division, polarity and cancer - e

... positions during development and are in close contact with neighbouring cells: their most recent daughters on the basal side (Truman and Bate, 1988), and cortex glial cells on their apical and lateral sides (Dumstrei et al., 2003). Moreover, NBs are not insensitive to their neighbours and are definit ...
The Arabidopsis sku6-spiral1 gene encodes a plus end
The Arabidopsis sku6-spiral1 gene encodes a plus end

... sku6-1 (Col)/spr1-1 (Ler) F1 plants. The SPR1 gene encodes a plant-specific 12-kD protein that is ubiquitously expressed and belongs to a six-member gene family in Arabidopsis. An SPR1:green fluorescent protein (GFP) fusion expressed in transgenic seedlings localized to microtubules within the corti ...
plant cell biology in the new millennium: new tools and new
plant cell biology in the new millennium: new tools and new

... Confocal microscopy—Confocal microscopy is proving to be one of the most exciting advances in optical microscopy of the last century. Although conventional wide-field epifluorescence microscopy has been a powerful tool for locating specific molecular components of the cell, it suffers from the probl ...
9/12
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... Basal body may rotate within a membrane bound ring of proteins (rotor and ...
Electron Microscopy of the Heart in a Case of Primary Cardiac
Electron Microscopy of the Heart in a Case of Primary Cardiac

... (1963) observed that some fibrils appeared to be composed of two filaments and that cross-striations appeared to be present. Shirahama and Cohen in seen. 1965 demonstrated by electron microscopy of negaOnly occasional capillaries were recognized, and tively stained amyloid that each fibril was compo ...
Halogens: Quaternary Disinfectant Oxidizing
Halogens: Quaternary Disinfectant Oxidizing

... • Damaging to some metals ...
Movements and stepwise fusion of endodermal precursor cells in
Movements and stepwise fusion of endodermal precursor cells in

... been evident to us and others from the fact that injected lineage tracers leak from one macromere into the others during stages 7–8. We have now found that the fusion of the macromeres occurs in a two-step process with a set time course. The first step is the fusion of A′′′ and B′′′ to form a cell w ...
Dynamical patterning modules in plant development and evolution
Dynamical patterning modules in plant development and evolution

role of potassium in human and animal nutrition 2
role of potassium in human and animal nutrition 2

... Kidneys do this by filtering the blood to remove the extra fluid and stores it as urine in the bladder. ...
Expression of Nuclear Lamin A and Muscle
Expression of Nuclear Lamin A and Muscle

... considerable changes in the expression of muscle-specific contractile proteins (Nadal-Ginard, 1978; Coleman and Coleman, 1968). The transcriptional activations of a number of muscle-specific genes are developmentally and coordinately regulated (Paterson and Bishop, 1977; Devlin and Emerson, 1978, 19 ...
Type III secretion: The bacteria-eukaryotic cell
Type III secretion: The bacteria-eukaryotic cell

... 2. The injectisome nanomachine The electron microscopy (EM) visualization of the first injectisome, from Salmonella enterica, was reported in 1998 [1]. Afterwards, EM studies allowed the visualization of the complete injectisomes from Shigella flexneri [2] and EPEC [3]. Recently, cryo-EM analyses allo ...
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Endomembrane system

The endomembrane system is composed of the different membranes that are suspended in the cytoplasm within a eukaryotic cell. These membranes divide the cell into functional and structural compartments, or organelles. In eukaryotes the organelles of the endomembrane system include: the nuclear membrane, the endoplasmic reticulum, the Golgi apparatus, lysosomes, vesicles, endosomes and the cell membrane. The system is defined more accurately as the set of membranes that form a single functional and developmental unit, either being connected directly, or exchanging material through vesicle transport. Importantly, the endomembrane system does not include the membranes of mitochondria or chloroplasts.The nuclear membrane contains two lipid bilayers that encompass the contents of the nucleus. The endoplasmic reticulum (ER) is a synthesis and transport organelle that branches into the cytoplasm in plant and animal cells. The Golgi apparatus is a series of multiple compartments where molecules are packaged for delivery to other cell components or for secretion from the cell. Vacuoles, which are found in both plant and animal cells (though much bigger in plant cells), are responsible for maintaining the shape and structure of the cell as well as storing waste products. A vesicle is a relatively small, membrane-enclosed sac that stores or transports substances. The cell membrane, is a protective barrier that regulates what enters and leaves the cell. There is also an organelle known as the Spitzenkörper that is only found in fungi, and is connected with hyphal tip growth.In prokaryotes endomembranes are rare, although in many photosynthetic bacteria the plasma membrane is highly folded and most of the cell cytoplasm is filled with layers of light-gathering membrane. These light-gathering membranes may even form enclosed structures called chlorosomes in green sulfur bacteria.The organelles of the endomembrane system are related through direct contact or by the transfer of membrane segments as vesicles. Despite these relationships, the various membranes are not identical in structure and function. The thickness, molecular composition, and metabolic behavior of a membrane are not fixed, they may be modified several times during the membrane's life. One unifying characteristic the membranes share is a lipid bilayer, with proteins attached to either side or traversing them.
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