Activating the DNA damage checkpoint in a developmental context
... nucleus, occur concurrently with S phase [6], much like in budding yeast. As in budding yeast, DNA damage during syncytial cycles delays not the entry into mitosis but the segregation of sister chromosomes during mitosis [7]. The mechanistic basis for this response remains to be understood. Syncytia ...
... nucleus, occur concurrently with S phase [6], much like in budding yeast. As in budding yeast, DNA damage during syncytial cycles delays not the entry into mitosis but the segregation of sister chromosomes during mitosis [7]. The mechanistic basis for this response remains to be understood. Syncytia ...
Flatworms and Roundworms
... long, tubular body, such as an earthworm. You might be less familiar with flatworms and roundworms. The flatworms are the largest group of acoelomate worms. Although the flatworm body plan is relatively simple, it is a great deal more complex than that of a sponge or cnidarian. Flatworms have a midd ...
... long, tubular body, such as an earthworm. You might be less familiar with flatworms and roundworms. The flatworms are the largest group of acoelomate worms. Although the flatworm body plan is relatively simple, it is a great deal more complex than that of a sponge or cnidarian. Flatworms have a midd ...
Subcellular Fractionation: What You Need to Know
... complexes require detailed observation/manipulation Impossible with whole cells. ...
... complexes require detailed observation/manipulation Impossible with whole cells. ...
A1982PM90100001
... periments showed that distilled water was preferable to buffers for release of folate from red cells. As with the serum folate assay, it was necessary to add ascorbic acid to protect the folate from oxidative destruction in subsequent autoclaving. We showed that one in ten dilution of whole blàod in ...
... periments showed that distilled water was preferable to buffers for release of folate from red cells. As with the serum folate assay, it was necessary to add ascorbic acid to protect the folate from oxidative destruction in subsequent autoclaving. We showed that one in ten dilution of whole blàod in ...
06Gastrulationtxt
... Gastrulation - The point in embryogenesis where the basic organization of the organism is established. ...
... Gastrulation - The point in embryogenesis where the basic organization of the organism is established. ...
Chapter 3 - PKDiet.com
... calcium and protein. These nutrients are also vital to the generation and utilization of hemoglobin, the energy courier of animal blood. The similarities between chlorophyll and heme are not limited to appearance and function. Chemists89 report that the synthesis of heme by animals can occur in much ...
... calcium and protein. These nutrients are also vital to the generation and utilization of hemoglobin, the energy courier of animal blood. The similarities between chlorophyll and heme are not limited to appearance and function. Chemists89 report that the synthesis of heme by animals can occur in much ...
Auxin Metabolism and Function in the Multicellular Brown Alga
... population. Nevertheless, the pattern is controlled by biological mechanisms, because statistical studies have identified several intrinsic constraints leading to a characteristic architecture (Le Bail et al., 2008a). Furthermore, modeling of these development steps indicates that local positional i ...
... population. Nevertheless, the pattern is controlled by biological mechanisms, because statistical studies have identified several intrinsic constraints leading to a characteristic architecture (Le Bail et al., 2008a). Furthermore, modeling of these development steps indicates that local positional i ...
Lab 5: Digestive, Respiratory, and Urogenital Systems of the Shark
... Vertebrates are coelomate organisms, meaning that they have a body cavity, or coelom. In the shark, the coelom is subdivided into a small cavity containing the heart, called the pericardial cavity (you ...
... Vertebrates are coelomate organisms, meaning that they have a body cavity, or coelom. In the shark, the coelom is subdivided into a small cavity containing the heart, called the pericardial cavity (you ...
11 May 2017 17:07 E.S.T. ASGCT Posters 2017
... The study of the human immune response to cancer is largely performed in a xenograft model in which human tumor and immune cells are transplanted into immunocompromised mice. This model is beneficial for assessing pre-clinical activity and safety of adoptive human T cell therapies. However, as the s ...
... The study of the human immune response to cancer is largely performed in a xenograft model in which human tumor and immune cells are transplanted into immunocompromised mice. This model is beneficial for assessing pre-clinical activity and safety of adoptive human T cell therapies. However, as the s ...
Hedgehog signaling and yolk sac angiogenesis
... neomycin resistant (neoR) cassette. The construct (St-Jacques et al., 1999) was modified to include a point mutation in the neoR cassette, making it less resistant to neomyocin (G418, Sigma). This modification facilitates the generation of homozygous transfectants using higher concentrations of G418 ...
... neomycin resistant (neoR) cassette. The construct (St-Jacques et al., 1999) was modified to include a point mutation in the neoR cassette, making it less resistant to neomyocin (G418, Sigma). This modification facilitates the generation of homozygous transfectants using higher concentrations of G418 ...
AUTOPHAGIC VACUOLES PRODUCED IN VITRO
... small vacuoles with an intermediate density between that of pinosomes and granules. Over the course of 1-2 hr these vacuoles grow larger and accumulate amorphous material or lipid. Pinosomes or granules frequently fuse with the toxic vacuoles. Chloroquine derivatives can be seen by fluorescence micr ...
... small vacuoles with an intermediate density between that of pinosomes and granules. Over the course of 1-2 hr these vacuoles grow larger and accumulate amorphous material or lipid. Pinosomes or granules frequently fuse with the toxic vacuoles. Chloroquine derivatives can be seen by fluorescence micr ...
EIN 3000: Introduction to Industrial Engineering and Management
... Identify the refracting media of the eye. Discuss the physiology of emmetropia, hypertropia and myopia. Define presbyopia, astigmatism, cataracts, and colorblindness. Discuss the advantage of binocular vision in humans. Discuss the results of damage to the afferent pathways of the eye. Given a coron ...
... Identify the refracting media of the eye. Discuss the physiology of emmetropia, hypertropia and myopia. Define presbyopia, astigmatism, cataracts, and colorblindness. Discuss the advantage of binocular vision in humans. Discuss the results of damage to the afferent pathways of the eye. Given a coron ...
Expression of the Catalytic and Regulatory Subunits of Protein
... acquisition of a mature phenotype resembling that of a granulocyte (17). The mature phenotype in HL-60 cells was confirmed by flow cytometric assessments with various monoclonal antibodies and an increased NBT reduction. HL-60 cell proliferation was inhibited after treatment with 1 ,.LMATRA. As show ...
... acquisition of a mature phenotype resembling that of a granulocyte (17). The mature phenotype in HL-60 cells was confirmed by flow cytometric assessments with various monoclonal antibodies and an increased NBT reduction. HL-60 cell proliferation was inhibited after treatment with 1 ,.LMATRA. As show ...
Nedd8 processing enzymes inSchizosaccharomyces pombe
... Background: Conjugation of the ubiquitin-like modifier Nedd8 to cullins is critical for the function of SCF-type ubiquitin ligases and thus facilitates ubiquitin conjugation and ultimately degradation of SCF substrates, including several cell cycle regulators. Like ubiquitin, Nedd8 is produced as a ...
... Background: Conjugation of the ubiquitin-like modifier Nedd8 to cullins is critical for the function of SCF-type ubiquitin ligases and thus facilitates ubiquitin conjugation and ultimately degradation of SCF substrates, including several cell cycle regulators. Like ubiquitin, Nedd8 is produced as a ...
Regulatory assembly of the vacuolar proton pump VOV1
... fluorescent proteins two different subunits. Cloning details will be published elsewhere. The mutants were labeled with the yeast-enhanced green fluorescent protein (yEGFP) which acts as the donor fluorophore in FRET imaging. We used mCherry as FRET acceptor fused to the E subunits. To prove FRET i ...
... fluorescent proteins two different subunits. Cloning details will be published elsewhere. The mutants were labeled with the yeast-enhanced green fluorescent protein (yEGFP) which acts as the donor fluorophore in FRET imaging. We used mCherry as FRET acceptor fused to the E subunits. To prove FRET i ...
IBCApplication_Revised02032015
... infected animals or tissues (including field work), recombinant or synthetic nucleic acid (rsNA) molecules, Select Agents & Toxins, and work with human blood, bodily fluids, tissues or cells in culture. Most of the biological research described in this application requires IBC authorization prior to ...
... infected animals or tissues (including field work), recombinant or synthetic nucleic acid (rsNA) molecules, Select Agents & Toxins, and work with human blood, bodily fluids, tissues or cells in culture. Most of the biological research described in this application requires IBC authorization prior to ...
Spirochaeta isovalerica sp. nov., a Marine Anaerobe That Forms
... (MGTY medium) (9). This medium was prepared as described previously (9), except that it included 5 ml of 1 M tris(hydroxymethy1)aminomethane hydrochloride buffer per 100 ml rather than 5 ml of 50 mM tris(hydroxymethy1)aminomethane hydrochloride buffer per 100 ml. For nutritional studies, S . isovale ...
... (MGTY medium) (9). This medium was prepared as described previously (9), except that it included 5 ml of 1 M tris(hydroxymethy1)aminomethane hydrochloride buffer per 100 ml rather than 5 ml of 50 mM tris(hydroxymethy1)aminomethane hydrochloride buffer per 100 ml. For nutritional studies, S . isovale ...
A parafusin-related Toxoplasma protein in Ca -regulated secretory organelles
... (PerkinElmer Life Sciences, Boston, MA/USA) using Esprit software (PerkinElmer Life Sciences). Deconvolution imaging was performed using a Provis AX70 microscope (Olympus) with a PlanApo 60 , 1.4 NA objective (Olympus) and High-Q filter sets (Chroma Technology). Images were acquired using a 15-bit ...
... (PerkinElmer Life Sciences, Boston, MA/USA) using Esprit software (PerkinElmer Life Sciences). Deconvolution imaging was performed using a Provis AX70 microscope (Olympus) with a PlanApo 60 , 1.4 NA objective (Olympus) and High-Q filter sets (Chroma Technology). Images were acquired using a 15-bit ...
View Full Page PDF - Physiological Reviews
... degree of selection and activation so that they are required only to facilitate further leukocyte migration and retention. ICAM-1 is well suited to mediate this process because nearly all immune cells constitutively express its receptors, i.e., the 2-integrins LFA-1 and Mac-1. In the case of T cell ...
... degree of selection and activation so that they are required only to facilitate further leukocyte migration and retention. ICAM-1 is well suited to mediate this process because nearly all immune cells constitutively express its receptors, i.e., the 2-integrins LFA-1 and Mac-1. In the case of T cell ...
Influence of salinity on the localization of Na+/K+
... of NKCC staining within the chloride cells (staining throughout the cell except for the nucleus and most apical region) was the same as for Na+/K+ATPase immunoreactivity. NKCC staining intensity was slightly higher in chloride cells on the primary filament compared with the secondary lamellae (Table ...
... of NKCC staining within the chloride cells (staining throughout the cell except for the nucleus and most apical region) was the same as for Na+/K+ATPase immunoreactivity. NKCC staining intensity was slightly higher in chloride cells on the primary filament compared with the secondary lamellae (Table ...
Bacteria and Archaea
... themselves with metabolic wastes, face competition from other microorganisms, or are consumed by other organisms. For example, the well-studied bacterium Escherichia coli can divide every 20 minutes under ideal lab conditions, one reason it is used as a model organism in research. However, when grow ...
... themselves with metabolic wastes, face competition from other microorganisms, or are consumed by other organisms. For example, the well-studied bacterium Escherichia coli can divide every 20 minutes under ideal lab conditions, one reason it is used as a model organism in research. However, when grow ...
Mesoderm and ectoderm lineages in the crustacean Parhyale
... studies raise the question of how early during development these cells are committed, if at all, to their subsequent germ layer fates and whether cell–cell interactions play a role in determining these fates. It is currently unknown to what extent maternal factors are unequally distributed within th ...
... studies raise the question of how early during development these cells are committed, if at all, to their subsequent germ layer fates and whether cell–cell interactions play a role in determining these fates. It is currently unknown to what extent maternal factors are unequally distributed within th ...
Retinoic acid-mediated activation of HNF-3a
... Xenopus proteins are clearly involved in early developmental decision processes, confirming that members of the HNF-3 family play a critical role in early development and are not restricted to the regulation of tissue-specific gene expression [31]. In this article, we have focused on the development ...
... Xenopus proteins are clearly involved in early developmental decision processes, confirming that members of the HNF-3 family play a critical role in early development and are not restricted to the regulation of tissue-specific gene expression [31]. In this article, we have focused on the development ...
1 Cell wall integrity controls root elongation via ACC Corresponding
... pathogen-associated molecular patterns (MAMPs) or elicitors (Gomez-Gomez 1999). We tested the short-term effect of flagellin-22 which is known to trigger defence responses in roots (Millet 2010). Flagellin reduced the LEH within 5h to about the same level as isoxaben at 3h. This reduction, too, was ...
... pathogen-associated molecular patterns (MAMPs) or elicitors (Gomez-Gomez 1999). We tested the short-term effect of flagellin-22 which is known to trigger defence responses in roots (Millet 2010). Flagellin reduced the LEH within 5h to about the same level as isoxaben at 3h. This reduction, too, was ...