Differential function of Listeria monocytogenes listeriolysin O and
... involved in the dissolution of the inner membrane of the spreading vacuole, yet are not sufficient for disruption of the outer membrane. As a consequence, we identified LLO as a key factor in the disruption of the outer membrane. This model is consistent with the observation that LLO is dispensable ...
... involved in the dissolution of the inner membrane of the spreading vacuole, yet are not sufficient for disruption of the outer membrane. As a consequence, we identified LLO as a key factor in the disruption of the outer membrane. This model is consistent with the observation that LLO is dispensable ...
040199 Management of Sickle Cell Disease
... standard care (16 percent), as compared with 1 of 63 children given prophylactic transfusions to reduce their hemoglobin S concentrations to below 30 percent (2 percent). Should all children with sickle cell anemia be screened — perhaps twice a year — for abnormalities in cerebrovascular blood flow ...
... standard care (16 percent), as compared with 1 of 63 children given prophylactic transfusions to reduce their hemoglobin S concentrations to below 30 percent (2 percent). Should all children with sickle cell anemia be screened — perhaps twice a year — for abnormalities in cerebrovascular blood flow ...
GRAM STAIN REAGENT TEST KIT
... approximately 0.4% crystal violet in an aqueous alcohol solution. Curtiss Gram Iodine Solution: Approximately 0.33% elemaental iodine in 0.66% aqueous potassium iodine solution. Curtiss Gram Decolorizer Solution: Gram Stain Decolorizer, denaturedethyl alcohol and acetone, approximately three parts t ...
... approximately 0.4% crystal violet in an aqueous alcohol solution. Curtiss Gram Iodine Solution: Approximately 0.33% elemaental iodine in 0.66% aqueous potassium iodine solution. Curtiss Gram Decolorizer Solution: Gram Stain Decolorizer, denaturedethyl alcohol and acetone, approximately three parts t ...
ultrasonic cleaning
... Ultrasonic is sound energy that creates cavitation when introduced into a liquid. The cavitation is caused by pressure differentials, providing the cleaning action of this process. In order to restore the anilox roll to full cell volume, a twostep ultrasonic cleaning process should be followed – soa ...
... Ultrasonic is sound energy that creates cavitation when introduced into a liquid. The cavitation is caused by pressure differentials, providing the cleaning action of this process. In order to restore the anilox roll to full cell volume, a twostep ultrasonic cleaning process should be followed – soa ...
Full-Text PDF
... species. For sterols separation the most commonly solvents for TLC on silica gel are these containing different proportions of chloroform-methanol-water [32]. In our study, a new specific thin layer chromatographic method was developed for determination of ergosterol and ergosterol peroxide in H. au ...
... species. For sterols separation the most commonly solvents for TLC on silica gel are these containing different proportions of chloroform-methanol-water [32]. In our study, a new specific thin layer chromatographic method was developed for determination of ergosterol and ergosterol peroxide in H. au ...
Journal of Biotechnology Rapid and highly efficient mammalian cell
... and gRNA complexes into C. elegans was first described in 2013 (Cho et al., 2013b) and was subsequently used to generate geneknockout mice and zebrafish with mutation rates of up to 93% in newborn mice (Sung et al., 2014). Following that report, Cas9 protein/gRNA RNP complexes were delivered into cult ...
... and gRNA complexes into C. elegans was first described in 2013 (Cho et al., 2013b) and was subsequently used to generate geneknockout mice and zebrafish with mutation rates of up to 93% in newborn mice (Sung et al., 2014). Following that report, Cas9 protein/gRNA RNP complexes were delivered into cult ...
03-Necrosis and apoptosis 2008
... Death of cells occurs in two ways: 1. Necrosis--(irreversible injury) changes produced by enzymatic digestion of dead cellular elements 2. Apoptosis--vital process that helps eliminate unwanted cells--an internally programmed series of events effected by dedicated gene products ...
... Death of cells occurs in two ways: 1. Necrosis--(irreversible injury) changes produced by enzymatic digestion of dead cellular elements 2. Apoptosis--vital process that helps eliminate unwanted cells--an internally programmed series of events effected by dedicated gene products ...
Full-Text PDF
... correlated with the incidence of pneumonia in healthy elderly individuals [17]. Other studies demonstrated that aged mice had increased lung inflammation and were found to be highly susceptible to pneumococcal pneumonia [18,19]. Furthermore, young mice infused with age-relevant physiological levels ...
... correlated with the incidence of pneumonia in healthy elderly individuals [17]. Other studies demonstrated that aged mice had increased lung inflammation and were found to be highly susceptible to pneumococcal pneumonia [18,19]. Furthermore, young mice infused with age-relevant physiological levels ...
preparing for icd-10-cm anatomy and pathophysiology training
... stratum spinosum layer where Langerhans cells are found along with many rows of spiny keratinocytes. The spines found here are cellular projections called desmosomes that form between keratinocytes to hold them together and resist friction. Just superficial to the stratum spinosum is the stratum gra ...
... stratum spinosum layer where Langerhans cells are found along with many rows of spiny keratinocytes. The spines found here are cellular projections called desmosomes that form between keratinocytes to hold them together and resist friction. Just superficial to the stratum spinosum is the stratum gra ...
- Columbia University Medical Center
... To generate SHH and its proteolytic products for use in induction assays, we transfected COS cells with cDNAderived expression plasmids (Figure 2B) that encode the wild-type mouse SHH, a SHH variant (SHH-UcA) in which the cleavage site cysteine residue has been changed to an alanine residue. This mu ...
... To generate SHH and its proteolytic products for use in induction assays, we transfected COS cells with cDNAderived expression plasmids (Figure 2B) that encode the wild-type mouse SHH, a SHH variant (SHH-UcA) in which the cleavage site cysteine residue has been changed to an alanine residue. This mu ...
PDF
... signalling in the early Xenopus embryo and in human HEK 293T cells. Gain- and loss-of-function experiments showed that xGalntl-1 interferes with the activity of the common TGF-β type II receptor ActR-IIB in vivo. In addition, our biochemical data demonstrated that xGalntl-1 specifically interferes w ...
... signalling in the early Xenopus embryo and in human HEK 293T cells. Gain- and loss-of-function experiments showed that xGalntl-1 interferes with the activity of the common TGF-β type II receptor ActR-IIB in vivo. In addition, our biochemical data demonstrated that xGalntl-1 specifically interferes w ...
Control of the Cell Cycle in Early Embryos
... At the end of oogenesis, oocytes exit from the cell cycle and arrest at the G2/M border of meiosis I. In clams, fertilization provides the extracellular signal that breaks this cell cycle arrest and initiates a series of rapid cell division cycles. In this paper, we consider two aspects of this proc ...
... At the end of oogenesis, oocytes exit from the cell cycle and arrest at the G2/M border of meiosis I. In clams, fertilization provides the extracellular signal that breaks this cell cycle arrest and initiates a series of rapid cell division cycles. In this paper, we consider two aspects of this proc ...
A model of chloroplast growth regulation in mesophyll cells
... Dockery and Keener (2001) based their quorum sensing model on the eight component gene-regulatory system described in Van Delden and Iglewski (1998). They simplified it down to two ordinary differential equations using time scale assumptions. We adopt the two variable model in this paper but conside ...
... Dockery and Keener (2001) based their quorum sensing model on the eight component gene-regulatory system described in Van Delden and Iglewski (1998). They simplified it down to two ordinary differential equations using time scale assumptions. We adopt the two variable model in this paper but conside ...
Programmed Cell Death in Neurons
... their trophic support in vivo. Difficulties encountered in this experimental model are those that are common to other primary neuronal culture paradigms, including the inability to obtain a large number of cells from each animal and the lack of an efficient transfection method to ectopically express ...
... their trophic support in vivo. Difficulties encountered in this experimental model are those that are common to other primary neuronal culture paradigms, including the inability to obtain a large number of cells from each animal and the lack of an efficient transfection method to ectopically express ...
Boron Functions in Plants: Looking Beyond the
... Katherine Warington (1923) is credited with establishing the boron (B) requirement for vascular plants. In her work with broad bean and other legumes, she determined that B must be supplied continuously during the life of the plant. Interestingly, she could not demonstrate a B requirement for the gr ...
... Katherine Warington (1923) is credited with establishing the boron (B) requirement for vascular plants. In her work with broad bean and other legumes, she determined that B must be supplied continuously during the life of the plant. Interestingly, she could not demonstrate a B requirement for the gr ...
CHAPTER 16MS Skin, Bones, and Muscles
... internal environment requires constant adjustments as conditions change inside and outside of cells. The endocrine system plays an important role in homeostasis because hormones, which are the messengers of the endocrine system, regulate the activity of body cells. The release of hormones into the b ...
... internal environment requires constant adjustments as conditions change inside and outside of cells. The endocrine system plays an important role in homeostasis because hormones, which are the messengers of the endocrine system, regulate the activity of body cells. The release of hormones into the b ...
FORMATION OF THE SCAPULAR PART OF THE PECTORAL
... wedge-shaped spaces are formed between the contacting structures (Kovalenko, 1992). In all the species studied the pronephros is relatively large during the whole premetamorphic development. It occupies the whole space between the ear capsules and abdominal cavity. Myomeres in these species are wide ...
... wedge-shaped spaces are formed between the contacting structures (Kovalenko, 1992). In all the species studied the pronephros is relatively large during the whole premetamorphic development. It occupies the whole space between the ear capsules and abdominal cavity. Myomeres in these species are wide ...
the Essential Standards of Biology
... teaching science, should provide opportunities for students to engage in “hands-on/minds-on” activities that are exemplars of scientific inquiry, experimentation and technological design. Science as Inquiry Traditional laboratory experiences provide opportunities to demonstrate how science is consta ...
... teaching science, should provide opportunities for students to engage in “hands-on/minds-on” activities that are exemplars of scientific inquiry, experimentation and technological design. Science as Inquiry Traditional laboratory experiences provide opportunities to demonstrate how science is consta ...
Individual-based modelling of growth and migration of Salmonella
... bacteria have penetrated the shell and shell membranes already. The albumen is assumed to be homogeneous, as is the yolk. Apart from viscosity differences, we are not aware of differences between the thick and thin albumen that may be relevant for bacterial growth or migration. From the viewpoint of ...
... bacteria have penetrated the shell and shell membranes already. The albumen is assumed to be homogeneous, as is the yolk. Apart from viscosity differences, we are not aware of differences between the thick and thin albumen that may be relevant for bacterial growth or migration. From the viewpoint of ...
Case Study - English
... when not using a guidewire to advance the catheter into the left atrium, which is in agreement with the manufacturer’s Instructions for Use. By using serial dilation of the femoral vein, we found a 10 Fr sheath advanced very easily. We did not have any difficulty achieving hemostasis after sheath re ...
... when not using a guidewire to advance the catheter into the left atrium, which is in agreement with the manufacturer’s Instructions for Use. By using serial dilation of the femoral vein, we found a 10 Fr sheath advanced very easily. We did not have any difficulty achieving hemostasis after sheath re ...
RNA Processing Bodies, Peroxisomes, Golgi Bodies, Mitochondria
... the RNA-processing body (P-body), stress granule, neuronal granule, and germ cell granule—are transported on microtubules (Hirokawa et al. 2009). In plants, RNA granules homologous to the stress granule and the P-body have been described (Bailey-Serres et al. 2009), but the involvement of the cytosk ...
... the RNA-processing body (P-body), stress granule, neuronal granule, and germ cell granule—are transported on microtubules (Hirokawa et al. 2009). In plants, RNA granules homologous to the stress granule and the P-body have been described (Bailey-Serres et al. 2009), but the involvement of the cytosk ...
PPT1 - Ycmou
... The intermediate filaments are more stable than actin and microtubules filaments. The intermediate filaments strengthen the cytoskeletons of cells in the tissues enabling the cells to withstand any mechanical stress in the tissues. Keratin filaments provide mechanical strength to epithelial cells in ...
... The intermediate filaments are more stable than actin and microtubules filaments. The intermediate filaments strengthen the cytoskeletons of cells in the tissues enabling the cells to withstand any mechanical stress in the tissues. Keratin filaments provide mechanical strength to epithelial cells in ...
The Polypeptide Composition of Moving and Stationary
... Schwob et al., 1986], though it can be upregulated in response to injury [Perlson et al., 2005]. Alpha-internexin, peripherin, NFL and NFM are not expressed until the onset of neuronal differentiation, with a-internexin often preceding the other three proteins [Kaplan et al., 1990; Troy et al., 1990 ...
... Schwob et al., 1986], though it can be upregulated in response to injury [Perlson et al., 2005]. Alpha-internexin, peripherin, NFL and NFM are not expressed until the onset of neuronal differentiation, with a-internexin often preceding the other three proteins [Kaplan et al., 1990; Troy et al., 1990 ...
42_lecture_presentation
... filtered fluid to blood maintains homeostasis. • This system aids in body defense. • Fluid, called lymph, reenters the circulation directly at the venous end of the capillary bed and indirectly through the lymphatic system. • The lymphatic system drains into neck veins. Copyright © 2008 Pearson Educ ...
... filtered fluid to blood maintains homeostasis. • This system aids in body defense. • Fluid, called lymph, reenters the circulation directly at the venous end of the capillary bed and indirectly through the lymphatic system. • The lymphatic system drains into neck veins. Copyright © 2008 Pearson Educ ...
nutrition kit contents - Seminary Of Natural Healing
... When dealing with nutrition, remember that people are unable to digest, absorb or assimilate nutrients because pathogens (bacteria, virus, parasite, fungus- found in Master kit) tend to interfere. Pathogen interference is involved more often than there is actual deficiency of the nutrient. This is w ...
... When dealing with nutrition, remember that people are unable to digest, absorb or assimilate nutrients because pathogens (bacteria, virus, parasite, fungus- found in Master kit) tend to interfere. Pathogen interference is involved more often than there is actual deficiency of the nutrient. This is w ...