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... List five body parts that you use when you drink a glass of water. Do you use any of those same body parts when you eat an apple? Do you use any of those same body parts when you pick up a heavy box? Record your response in your science notebook. ...
Transient Recombinant Protein Expression in Mammalian Cells
Transient Recombinant Protein Expression in Mammalian Cells

... the rapid production of milligram to gram amounts of recombinant proteins. This review describes the different methods for introducing foreign DNA into suitable mammalian cells with either viral or non-viral vectors. Particular emphasis is given to non-viral transient transfection which represents m ...
Bacterial flagella explore microscale hummocks and hollows to
Bacterial flagella explore microscale hummocks and hollows to

... roughness on the bacterial length scale provide less available surface area and fewer attachment points for rigid bacterial bodies than smooth surfaces (Fig. 1A). However, the simplistic view of bacteria as rigid rods or spheres ignores the presence of bacterial appendages, such as pili and flagella ...
Urogential System
Urogential System

... 2. With forceps, lift the loose skin of the abdomen. Carefully insert the tip of a pair of scissors beneath the skin. Cut away from your body. Cut along line AB as shown above. Continue cutting lines CD and EF. Be sure not to cut deeper than the skin. 3. With your fingers and the probe, separate the ...
Calcium-Containing Organelles Display Unique Reactivity to
Calcium-Containing Organelles Display Unique Reactivity to

... 300 msec pulse, n 5 11 cells) caused a transient rise in [Ca]i, which initially started in CCOs (see the insert amplification of the first part of the graph) and was larger in the nucleus than in cytoplasm or the CCOs, and a slower recovery to baseline in the latter than in former two compartments. ...
Lymphatic endothelial differentiation: start out with Sox
Lymphatic endothelial differentiation: start out with Sox

... causing lymphatic defects in the mouse have led to unprecedented progress in our understanding of the biology of lymph vessel formation. ...
Smectic-A-filled birefringent elements and fast switching twisted dual
Smectic-A-filled birefringent elements and fast switching twisted dual

... 共8OCB兲 in the planar cell with polyisoprene-coated substrates34 results in layer undulations and formation of FCDs clearly visible in Fig. 2. These FCDs can be stabilized by a mechanical impurity in the bulk or at the surface of the cell.30 In principle, one can use a magnetic field to align the SmA ...
Sec35p, a Novel Peripheral Membrane Protein, Is Required for ER
Sec35p, a Novel Peripheral Membrane Protein, Is Required for ER

... analyses. The SEC35 deletion strain, GWY128, was constructed using the g-transformation method of one-step gene replacement (for review see Rose, 1995) in which the complete open reading frame (ORF) is replaced by sequences contained in the yeast integrating vector, pRS305 (Sikorski and Hieter, 1989 ...
Vertebrate$`Relationships
Vertebrate$`Relationships

... more commonly known as amphioxus. (Greek amphi = both and oxy = sharp; amphioxus means "sharp at both ends," an appropriate term for an animal in which the front and rear ends are nearly the same shape because it lacks a distinct head.) Lancelets are widely distributed in marine waters of the contin ...
Muscle tissues
Muscle tissues

... at the periphery of the cell, just beneath the thin, elastic membrane (sarcolemma). The sarcoplasm also has many alternating light and dark bands, giving the fibre a striped or striated appearance (hence the name striated muscle). With the aid of an electron microscope it can be seen that each muscl ...
Feeding in a Calcarous Sponge: Particle
Feeding in a Calcarous Sponge: Particle

... At the apical surface each choanocyte has a 15 µm-long flagellum that is surrounded by a ring of microvilli (Fig. 2 E,F). The shape of collar microvilli was highly variable in all sponges; while some collar microvilli were long and thin (2-3 µm long, 0.1 µm in diameter) and individual microvilli wer ...
Original Article Prediction of non-small cell lung cancer metastasis
Original Article Prediction of non-small cell lung cancer metastasis

... cells penetrate the endothelial basement membrane into the lymphatic vessels and blood vessels, migrate to new organs via the circulation, attach to vascular endothelial cells and stick out to form metastatic loci. Many mechanisms including factors-promoting mechanism, signal pathway mechanism, adhe ...
Glucose-6-phosphate Metabolic Preferential Destinations
Glucose-6-phosphate Metabolic Preferential Destinations

... Background: The oviduct is a dynamic organ which facilitates gamete function, fertilization and embryo development. This organ is covered by an epithelium containing ciliated and non-ciliated cells. Secretions of non-ciliated cells compose the oviduct fluid, which will nourish the early embryo. Duri ...
Harmonin Mutations Cause Mechanotransduction Defects in
Harmonin Mutations Cause Mechanotransduction Defects in

... hair cell. However, which molecules mediate cadherin function at tip links is not known. Here we show that the PDZ-domain protein harmonin is a component of the upper tip-link density (UTLD), where CDH23 inserts into the stereociliary membrane. Harmonin domains that mediate interactions with CDH23 a ...
Evaluating hypotheses for the origin of eukaryotes
Evaluating hypotheses for the origin of eukaryotes

... hypotheses attempt to account for these patterns but disagree about the nature of the host and the number of partners involved in the origin of the eukaryote cell. Here we consider four general models (Fig. 1). These are not exhaustive, but instead aim to generalise the main features of a large numb ...
MUC1 is a novel costimulatory molecule of human T cells and
MUC1 is a novel costimulatory molecule of human T cells and

... signaling motifs [2] and is noncovalently linked to the extracellular domain [3]. Recent studies have suggested that it migrates to the nucleus upon extracellular ligation in epithelial cells, acting as a shuttle protein for transcription factors such as ␤-catenin [4], and can also generate both sti ...
Modulating Wound Healing After Glaucoma Surgery
Modulating Wound Healing After Glaucoma Surgery

... complications.3 Moreover, because surgery can fail despite the use of powerful antimetabolites, there is a need for better agents with improved risk-benefit ratios. Controlling the scarring process could theoretically deliver postoperative IOPs around 10 mm Hg in all patients. Many methods now exist ...
Significance of hydrolytic enzymes expressed during xylem
Significance of hydrolytic enzymes expressed during xylem

... conducting cells lacked secondary thickenings (Edwards 1986). As the protoplast of a living cell greatly impedes water conductance (Raven 1993), it can be assumed that those structures were dead empty cell corpses. Conduits that are supported by secondary cell wall thickenings, the characteristic of ...
ASYMMETRIC CELL DIVISION IN PLANTS
ASYMMETRIC CELL DIVISION IN PLANTS

... In the budding yeast Saccharomyces cerevisiae, cells of two mating types exist. Upon spore germination, cell division is asymmetric: It gives rise to a “mother” cell capable of mating-type switching during the next cell cycle, and a “daughter” cell—which originates as a growing bud on the mother cel ...
The origins, function, and regulation of T follicular helper cells
The origins, function, and regulation of T follicular helper cells

... by B cells drives the full development of Tfh cells. (iii) Within GC, Tfh cells continue to provide help to the B cells, supporting the GC reaction and facilitating the generation of long-lived plasma cells and memory B cells. Reciprocal signals provided by the B cells are also crucial for sustainin ...
Reduced red blood cell velocity in nail
Reduced red blood cell velocity in nail

... fibrosis; oesophagus ¼ hypomotility shown by barium radiography; and kidney ¼ malignant hypertension and rapidly progressive renal failure without any other explanation. Pulmonary function tests, including vital capacity (VC) and diffusion capacity for carbon monoxide (DLCO), were also performed. Wh ...
I inhibition: a novel mechanism of action
I inhibition: a novel mechanism of action

... Servier, Neuilly-sur-Seine, France), a recently developed molecule, blocks If channels when they are open and preferentially when the current is outward. Conclusions If controls the slope of diastolic depolarization and cardiac frequency, and its inhibition causes heart rate reduction. The current-d ...
NG2 glial cells integrate synaptic input in global and dendritic
NG2 glial cells integrate synaptic input in global and dendritic

... additional analysis including all dendrite recordings. We binned trials according to stimulus 8 of 43 ...
Production of fibronectin and collagen types I and III by chick embryo
Production of fibronectin and collagen types I and III by chick embryo

... of the culture media, it was shown that. after 7 days of culture. cells secreted more type I than type III collagen. Type I collagen production Increased with tIme of culture. Substrates consisting of type IV collagen or GAGs (alone or In mixtures with type I collagen) exerted an inhibitory Influenc ...
Molecular mechanism of protrusion formation during Listeria Keith Ireton Luciano A. Rigano
Molecular mechanism of protrusion formation during Listeria Keith Ireton Luciano A. Rigano

... e-mail: wolf-dieter.schubert@ up.ac.za ...
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Organ-on-a-chip

An organ-on-a-chip (OC) is a multi-channel 3-D microfluidic cell culture chip that simulates the activities, mechanics and physiological response of entire organs and organ systems. It constitutes the subject matter of significant biomedical engineering research, more precisely in bio-MEMS. The convergence of labs-on-chips (LOCs) and cell biology has permitted the study of human physiology in an organ-specific context, introducing a novel model of in vitro multicellular human organisms. One day, they will perhaps abolish the need for animals in drug development and toxin testing.Although multiple publications claim to have translated organ functions onto this interface, the movement towards this microfluidic application is still in its infancy. Organs-on-chips will vary in design and approach between different researchers. As such, validation and optimization of these systems will likely be a long process. Organs that have been simulated by microfluidic devices include the heart, the lung, kidney, artery, bone, cartilage, skin and more.Nevertheless, building valid artificial organs requires not only a precise cellular manipulation, but a detailed understanding of the human body’s fundamental intricate response to any event. A common concern with organs-on-chips lies in the isolation of organs during testing. ""If you don’t use as close to the total physiological system that you can, you’re likely to run into troubles"" says William Haseltine, founder of Rockville, Maryland. Microfabrication, microelectronics and microfluidics offer the prospect of modeling sophisticated in vitro physiological responses under accurately simulated conditions.
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