Mitochondrial Dynamics
... 2.2.1 Animal and Yeast Mitochondrial Division Mitochondrial division is a highly coordinated process that involves the formation of two or more daughter organelles by scission of a single mitochondrion. To an extent the mechanisms that carry out mitochondrial fission reflect the evolutionary histor ...
... 2.2.1 Animal and Yeast Mitochondrial Division Mitochondrial division is a highly coordinated process that involves the formation of two or more daughter organelles by scission of a single mitochondrion. To an extent the mechanisms that carry out mitochondrial fission reflect the evolutionary histor ...
Host cell interactions of outer membrane vesicle-associated
... endothelial cells (HBMEC), and renal glomerular endothelial cells (HRGEC) in a timedependent manner (Fig 2A, 2D and 2G). There were no significant differences between OMVs from NSF (5791/99) and SF (493/89) EHEC O157 strains, and OMVs containing (5791/99, 493/89) and lacking (493/89Δstx2a) Stx2a (Fi ...
... endothelial cells (HBMEC), and renal glomerular endothelial cells (HRGEC) in a timedependent manner (Fig 2A, 2D and 2G). There were no significant differences between OMVs from NSF (5791/99) and SF (493/89) EHEC O157 strains, and OMVs containing (5791/99, 493/89) and lacking (493/89Δstx2a) Stx2a (Fi ...
Modes of anaerobic respiration catalysed by
... allow for improved fate prediction of halogenated compounds from anthropogenic or natural sources in the environment. Reductive dehalogenation of brominated aromatics was also shown to occur in marine deep-sea sediment microcosms with activity assays, inhibition studies and cultivation experiments a ...
... allow for improved fate prediction of halogenated compounds from anthropogenic or natural sources in the environment. Reductive dehalogenation of brominated aromatics was also shown to occur in marine deep-sea sediment microcosms with activity assays, inhibition studies and cultivation experiments a ...
Natalia Gromak, Alexis Rideau,
... tissue samples, and were undetectable at PCR cycle numbers necessary to prevent saturation of the PCR from tissue samples. However, higher cycle numbers of PCR and western blot (see below) showed that raver1 is expressed in PAC-1 cells. Having established that raver1 is expressed in tissues where TM ...
... tissue samples, and were undetectable at PCR cycle numbers necessary to prevent saturation of the PCR from tissue samples. However, higher cycle numbers of PCR and western blot (see below) showed that raver1 is expressed in PAC-1 cells. Having established that raver1 is expressed in tissues where TM ...
Characterization of the Visceral Endoderm Components in Early
... period of embryonic development in which the cells undergo complex morphological movements to generate the three primary germ layers (ectoderm, mesoderm and endoderm), from which the whole fetus develops later. (Snow and Bennett, 1978; Skreb et al., 1991; Tam et al., 2006; Tam and Loebel, 2007; Soln ...
... period of embryonic development in which the cells undergo complex morphological movements to generate the three primary germ layers (ectoderm, mesoderm and endoderm), from which the whole fetus develops later. (Snow and Bennett, 1978; Skreb et al., 1991; Tam et al., 2006; Tam and Loebel, 2007; Soln ...
Microfluidic based Sample Preparation for Bloodstream Infections
... (bacteremia) are the leading cause [21]. SIRS patients may display a range of different clinical manifestations such as; fever (> 38°C), rapid heart rate (> 90 beats/min), hyperventilation and changes in white blood-cell counts [22,25,28,29]. Two or more of these signs are needed to fulfill the crit ...
... (bacteremia) are the leading cause [21]. SIRS patients may display a range of different clinical manifestations such as; fever (> 38°C), rapid heart rate (> 90 beats/min), hyperventilation and changes in white blood-cell counts [22,25,28,29]. Two or more of these signs are needed to fulfill the crit ...
–plasma-membrane junctions by Kv2.1 Induction of stable ER potassium channels
... that Kv2.1 induces a true ER–plasma-membrane junction with a <20 nm gap between the two membranes (Carrasco and Meyer, 2011; Orci et al., 2009). Thus, in order to examine the spatial relationship between Kv2.1 clusters on the plasma membrane and the underlying cER at high resolution, we used immuno- ...
... that Kv2.1 induces a true ER–plasma-membrane junction with a <20 nm gap between the two membranes (Carrasco and Meyer, 2011; Orci et al., 2009). Thus, in order to examine the spatial relationship between Kv2.1 clusters on the plasma membrane and the underlying cER at high resolution, we used immuno- ...
Exosomes: Implications in HIV-1 Pathogenesis
... to present major histocompatibility class II—antigen complexes to T lymphocytes [4]. Furthermore, in 2007, exosomes from human and murine mast cells were discovered to contain functional mRNA and miRNA that could be donated to recipient cells and translated into heterologous or homologous proteins [ ...
... to present major histocompatibility class II—antigen complexes to T lymphocytes [4]. Furthermore, in 2007, exosomes from human and murine mast cells were discovered to contain functional mRNA and miRNA that could be donated to recipient cells and translated into heterologous or homologous proteins [ ...
Class II Histone Deacetylases Are Directly Recruited by BCL6
... that is a 50/50 mixture of Dulbecco's modified Eagle's/Ham's F-12 medium (Invitrogen) containing 10% fetal calf serum (Sigma-Aldrich) and antibiotics. For UTA-L cells, growth medium was supplemented with tetracycline (Sigma-Aldrich, 2 µg/ml), G418 (Invitrogen, 500 µg/ml), and puromycine (Sigma-Aldri ...
... that is a 50/50 mixture of Dulbecco's modified Eagle's/Ham's F-12 medium (Invitrogen) containing 10% fetal calf serum (Sigma-Aldrich) and antibiotics. For UTA-L cells, growth medium was supplemented with tetracycline (Sigma-Aldrich, 2 µg/ml), G418 (Invitrogen, 500 µg/ml), and puromycine (Sigma-Aldri ...
Origins of DNA Replication - DNA Replication and Human Disease
... acting in concert to determine where and when DNA replication will occur (Table 1). In this sense, replication origins are equivalent to transcription promoters, but whereas each promoter is uniquely responsible for transcription of its associated gene, large chromosomes like yeast often contain mor ...
... acting in concert to determine where and when DNA replication will occur (Table 1). In this sense, replication origins are equivalent to transcription promoters, but whereas each promoter is uniquely responsible for transcription of its associated gene, large chromosomes like yeast often contain mor ...
Secretion-Defective Mutations in the Signal Sequence for Saccharomyces cerevisiae Invertase.
... were used. To generate deletions by the limited digestion of duplex DNA, 1,500 U of Si per ml were used. All digestions were carried out for 20 min at 25°C. Digestion of DNA by T4 DNA polymerase was done in 33 mM Tris acetate (pH 7.9)-66 mM potassium acetate-10 mM magnesium acetate-0.5 mM dithiothre ...
... were used. To generate deletions by the limited digestion of duplex DNA, 1,500 U of Si per ml were used. All digestions were carried out for 20 min at 25°C. Digestion of DNA by T4 DNA polymerase was done in 33 mM Tris acetate (pH 7.9)-66 mM potassium acetate-10 mM magnesium acetate-0.5 mM dithiothre ...
Wood - 2010 - Nat Rev Genet
... sister chromatids, which are held together by sister chromatid cohesion (SCC). SCC is mediated by the cohesin complex, which associates with chromosomes before their replication and is converted into a cohesive state as replication forks pass. Therefore, sister chromatids are held together by SCC co ...
... sister chromatids, which are held together by sister chromatid cohesion (SCC). SCC is mediated by the cohesin complex, which associates with chromosomes before their replication and is converted into a cohesive state as replication forks pass. Therefore, sister chromatids are held together by SCC co ...
regionally specific induction by the spemann
... organizers on the basis of their different inducing abilities. The various models that were historically put forward to explain regionally specific induction by these different organizers can be divided into two classes. Quantitative models proposed gradients of single inducers, such as the elusive ...
... organizers on the basis of their different inducing abilities. The various models that were historically put forward to explain regionally specific induction by these different organizers can be divided into two classes. Quantitative models proposed gradients of single inducers, such as the elusive ...
Effects of GDF-9 and FSH on in vitro development of bovine
... Experiment 2: Assessment of interaction between BMP4 and FSH on cultured secondary follicles To investigate a possible interaction between BMP-4 and FSH, the concentration of BMP-4 that had the best results in experiment 1 was used to culture secondary follicles for a longer culture period (18 days) ...
... Experiment 2: Assessment of interaction between BMP4 and FSH on cultured secondary follicles To investigate a possible interaction between BMP-4 and FSH, the concentration of BMP-4 that had the best results in experiment 1 was used to culture secondary follicles for a longer culture period (18 days) ...
Evidence that FGF8 signalling from the midbrain
... Martinez et al., 1991; Marin and Puelles, 1994). Together, these data implicate isthmic derived signals in the establishment of mesencephalic rostrocaudal polarity and imply that such signals may act via the regulation of En expression. Recent experiments have demonstrated the importance of FGF8 in ...
... Martinez et al., 1991; Marin and Puelles, 1994). Together, these data implicate isthmic derived signals in the establishment of mesencephalic rostrocaudal polarity and imply that such signals may act via the regulation of En expression. Recent experiments have demonstrated the importance of FGF8 in ...
Journal of Bacteriology
... Interactions of pseudobactin 358 with deleterious and pathogenic rhizosphere microorganisms. In an antibiosis assay (Fig. 3), the cyanide-producing Pseudomonas strains All, A14, and A63, the pathogenic bacteria E. carotovora subsp. carotovora and E. carotovora subsp. atroseptica, and the pathogenic ...
... Interactions of pseudobactin 358 with deleterious and pathogenic rhizosphere microorganisms. In an antibiosis assay (Fig. 3), the cyanide-producing Pseudomonas strains All, A14, and A63, the pathogenic bacteria E. carotovora subsp. carotovora and E. carotovora subsp. atroseptica, and the pathogenic ...
A Comprehensive Mutational Analysis of the
... A previous study on intraspecific polymorphism at RPW8.2 showed that compared with the functional RPW8.2 allele from accession Ms-0 (designated R82Ms-0), Thr-64 to Ser (T64S), Asp-116 to Gly (D116G), and Thr-161 to Lys (T161K) substitutions are present in alleles from most accessions that are suscept ...
... A previous study on intraspecific polymorphism at RPW8.2 showed that compared with the functional RPW8.2 allele from accession Ms-0 (designated R82Ms-0), Thr-64 to Ser (T64S), Asp-116 to Gly (D116G), and Thr-161 to Lys (T161K) substitutions are present in alleles from most accessions that are suscept ...
Ribosomes slide on lysine-encoding homopolymeric A stretches
... eLife digest Genes provide the instructions to assemble proteins from smaller molecules called amino acids. When a gene is ‘switched on’, the DNA that makes up the gene is copied into messenger ribonucleic acid (or mRNA) molecules, composed of building blocks called nucleotides. There are four types ...
... eLife digest Genes provide the instructions to assemble proteins from smaller molecules called amino acids. When a gene is ‘switched on’, the DNA that makes up the gene is copied into messenger ribonucleic acid (or mRNA) molecules, composed of building blocks called nucleotides. There are four types ...
Regulation of nuclear poly(A) addition controls the expression of
... (lowest mutant to wild-type ratio) while the immature B cells (WEHI) show the highest ratio. The luciferase activity from constructs bearing the mutated motifs is similar in all three cells lines; the difference lies in those bearing wild-type sequences (Figure 2C). This shows that these sequences i ...
... (lowest mutant to wild-type ratio) while the immature B cells (WEHI) show the highest ratio. The luciferase activity from constructs bearing the mutated motifs is similar in all three cells lines; the difference lies in those bearing wild-type sequences (Figure 2C). This shows that these sequences i ...
The versatility of mitochondrial calcium signals: From stimulation of
... ∼ 0.3 mM and rapidly emptying after agonist stimulation) [16], the rapid equilibration of cytosolic and nuclear [Ca2+] [17], and the estimates of resting and stimulated [Ca2+]c under the plasma membrane, which is well above those of the bulk cytosol [18]. The present review focuses on mitochondria, ...
... ∼ 0.3 mM and rapidly emptying after agonist stimulation) [16], the rapid equilibration of cytosolic and nuclear [Ca2+] [17], and the estimates of resting and stimulated [Ca2+]c under the plasma membrane, which is well above those of the bulk cytosol [18]. The present review focuses on mitochondria, ...
Snake venomics of Bothrops punctatus
... The significance of differences between means was assessed by ANOVA, followed by Dunnett’s test, when several experimental groups were compared with the control group, or by Student’s t-test, when two groups were compared. Differences were considered significant if p < 0.05. ...
... The significance of differences between means was assessed by ANOVA, followed by Dunnett’s test, when several experimental groups were compared with the control group, or by Student’s t-test, when two groups were compared. Differences were considered significant if p < 0.05. ...
Preprint - Columbia Statistics
... The inputs to the model are xt , nt and lt , which represent discrete time series for the RGC spikes (S potentials), LGN spikes, and the luminance of the visual stimulus at time t, respectively. A distinct linear temporal filter is convolved with each ~ acts on the past LGN spikes (nt ) and source o ...
... The inputs to the model are xt , nt and lt , which represent discrete time series for the RGC spikes (S potentials), LGN spikes, and the luminance of the visual stimulus at time t, respectively. A distinct linear temporal filter is convolved with each ~ acts on the past LGN spikes (nt ) and source o ...
Nitric oxide cell signaling: S-nitrosation of Ras superfamily GTPases
... thiols were not subject to free radical induced GNE nor produced an EPR detectable thiyl radical signal. Of note, Ras nitrosated at Cys118 has been shown to exhibit similar structure and GNE rates as the non-nitrosated form of Ras [21]. Contrary to the belief that S-nitrosation leads to an activated ...
... thiols were not subject to free radical induced GNE nor produced an EPR detectable thiyl radical signal. Of note, Ras nitrosated at Cys118 has been shown to exhibit similar structure and GNE rates as the non-nitrosated form of Ras [21]. Contrary to the belief that S-nitrosation leads to an activated ...
Glucose transporters - Cambridge University Press
... Abel et al. 2001) exhibit a varying degree of pathophysiology relating to type II diabetes. Charron and colleagues (Katz et al. 1995, 1996) have suggested that, amongst other factors, the expression of additional but unknown glucose transporters may have a role in the observed phenotypes. Compensato ...
... Abel et al. 2001) exhibit a varying degree of pathophysiology relating to type II diabetes. Charron and colleagues (Katz et al. 1995, 1996) have suggested that, amongst other factors, the expression of additional but unknown glucose transporters may have a role in the observed phenotypes. Compensato ...
Pepsin-Mediated Processing of the Cytoplasmic Histone H2A to
... encode antimicrobial peptides is tissue specific. Antimicrobial peptides can function intracellularly, as in circulating leukocytes or in the external environment after release by secretory cells and other granulated epithelia (8). Certain antimicrobial peptides have diverse functions beyond microbi ...
... encode antimicrobial peptides is tissue specific. Antimicrobial peptides can function intracellularly, as in circulating leukocytes or in the external environment after release by secretory cells and other granulated epithelia (8). Certain antimicrobial peptides have diverse functions beyond microbi ...