Gain-of-Function Mutations in SCN5A Gene Lead to Type
... alpha subunit (SCN5A) mutation, has been identified since 1995. LQTS mutation in SCN5A is a gain-of-function mutation producing late sodium current, INa,L. Brugada mutation in SCN5A is a loss-of-function causing INa decrease. Whereas, the mechanism for Dilated Cardiomyopathy mutations in SCN5A is st ...
... alpha subunit (SCN5A) mutation, has been identified since 1995. LQTS mutation in SCN5A is a gain-of-function mutation producing late sodium current, INa,L. Brugada mutation in SCN5A is a loss-of-function causing INa decrease. Whereas, the mechanism for Dilated Cardiomyopathy mutations in SCN5A is st ...
The symbiotic ion channel homolog DMI1 is localized in the nuclear
... Previous reports indicated that CASTOR and POLLUX, the DMI1 homologs from L. japonicus, contain transit peptides that target these proteins to root plastids. We analyzed the DMI1 amino acid sequence in silico using multiple online prediction programs and each gave conflicting results. Although Chlor ...
... Previous reports indicated that CASTOR and POLLUX, the DMI1 homologs from L. japonicus, contain transit peptides that target these proteins to root plastids. We analyzed the DMI1 amino acid sequence in silico using multiple online prediction programs and each gave conflicting results. Although Chlor ...
Low peak bone mass and attenuated anabolic response to
... Development of alkaline phosphatase activity, a marker of osteogenic differentiation, was significantly reduced in calvarial cells derived from ColCre;Cx43–/fl mice 2 weeks postconfluence, when it usually reaches a peak, as it occurred in the other two genotypes (Fig. 4D). Furthermore, after 2 weeks ...
... Development of alkaline phosphatase activity, a marker of osteogenic differentiation, was significantly reduced in calvarial cells derived from ColCre;Cx43–/fl mice 2 weeks postconfluence, when it usually reaches a peak, as it occurred in the other two genotypes (Fig. 4D). Furthermore, after 2 weeks ...
UvA-DARE (Digital Academic Repository) HIV
... of immune cells in the subepithelial lamina propria, it is thought that macrophages play a role in transmission of the virus from host-to-host16. Organ culture systems derived from cervical tissue allowed for the identification of CD4+ T cells as the first cells being infected after contact with cel ...
... of immune cells in the subepithelial lamina propria, it is thought that macrophages play a role in transmission of the virus from host-to-host16. Organ culture systems derived from cervical tissue allowed for the identification of CD4+ T cells as the first cells being infected after contact with cel ...
Low peak bone mass and attenuated anabolic response to
... Development of alkaline phosphatase activity, a marker of osteogenic differentiation, was significantly reduced in calvarial cells derived from ColCre;Cx43–/fl mice 2 weeks postconfluence, when it usually reaches a peak, as it occurred in the other two genotypes (Fig. 4D). Furthermore, after 2 weeks ...
... Development of alkaline phosphatase activity, a marker of osteogenic differentiation, was significantly reduced in calvarial cells derived from ColCre;Cx43–/fl mice 2 weeks postconfluence, when it usually reaches a peak, as it occurred in the other two genotypes (Fig. 4D). Furthermore, after 2 weeks ...
Anterior PAR proteins function during cytokinesis and
... and Hyman, 2004; Macara, 2004; Shivas et al., 2010], but their function during cell division remains elusive. Cell polarity is naturally coupled with many events that occur during cell division. As a tissue proliferates and develops, each cell must maintain its polarization state upon each division ...
... and Hyman, 2004; Macara, 2004; Shivas et al., 2010], but their function during cell division remains elusive. Cell polarity is naturally coupled with many events that occur during cell division. As a tissue proliferates and develops, each cell must maintain its polarization state upon each division ...
aureus Staphylococcus MyD88-Dependent Responses to Are
... secretors of proinflammatory cytokines. The inflammatory response to pathogens is triggered by pattern recognition receptors (PRRs), such as the TLRs that initiate inflammatory-signaling cascades (8, 9). Although these receptors are highly expressed by phagocytic innate immune cells, it is clear tha ...
... secretors of proinflammatory cytokines. The inflammatory response to pathogens is triggered by pattern recognition receptors (PRRs), such as the TLRs that initiate inflammatory-signaling cascades (8, 9). Although these receptors are highly expressed by phagocytic innate immune cells, it is clear tha ...
Serum Factors from Pseudoxanthoma Elasticum
... specific to elastin and fibrillin 1 revealed that PXE fibroblasts maintained in the presence of 10% FBS produced an abundant network of elastic fibers (Figure 1b and d). The distribution pattern of these elastic fibers did not appreciably differ from those observed in cultures of normal cells (Figur ...
... specific to elastin and fibrillin 1 revealed that PXE fibroblasts maintained in the presence of 10% FBS produced an abundant network of elastic fibers (Figure 1b and d). The distribution pattern of these elastic fibers did not appreciably differ from those observed in cultures of normal cells (Figur ...
"Yeast". - ResearchGate
... artificial chromosomes that can be used to study various aspects of chromosome behavior, such as how chromosomes pair and segregate from each other during mitosis and meiosis. In addition, systems using artificial chromosomes have been designed that allow cloning of larger contiguous segments of DN ...
... artificial chromosomes that can be used to study various aspects of chromosome behavior, such as how chromosomes pair and segregate from each other during mitosis and meiosis. In addition, systems using artificial chromosomes have been designed that allow cloning of larger contiguous segments of DN ...
Clostridium and Bacillus Binary Enterotoxins
... (C. perfringens enterotoxin or CPE) is also mouse lethal, causes erythema in guinea pigs, and linked to a major form of food poisoning found throughout the world [28,29]. Genetic methods involving multiplex PCR are now more commonly used than animal assays by many diagnostic laboratories for toxin t ...
... (C. perfringens enterotoxin or CPE) is also mouse lethal, causes erythema in guinea pigs, and linked to a major form of food poisoning found throughout the world [28,29]. Genetic methods involving multiplex PCR are now more commonly used than animal assays by many diagnostic laboratories for toxin t ...
Mechanisms of plasmid stable maintenance with special focus on
... the probability of receiving a plasmid by a dividing cell there are very special strategies adopted by plasmids preventing plasmid-free segregants from surviving. The idea of an “addiction” mechanism leading to very efficient plasmid maintenance comes from Koyama (Koyama et al., 1975). In his consid ...
... the probability of receiving a plasmid by a dividing cell there are very special strategies adopted by plasmids preventing plasmid-free segregants from surviving. The idea of an “addiction” mechanism leading to very efficient plasmid maintenance comes from Koyama (Koyama et al., 1975). In his consid ...
Full Text - Labs / Projects - Fred Hutchinson Cancer Research Center
... homozygous for a deficiency that includes the Rho1 locus exhibit no staining above background, compared with sibling controls (Fig. 1C,D). We also overexpressed Rho1, Rac1, and Cdc42 in embryos using the conditional Gal4-UAS system. An Engrailed-Gal4 driver was used to express the Rho proteins in st ...
... homozygous for a deficiency that includes the Rho1 locus exhibit no staining above background, compared with sibling controls (Fig. 1C,D). We also overexpressed Rho1, Rac1, and Cdc42 in embryos using the conditional Gal4-UAS system. An Engrailed-Gal4 driver was used to express the Rho proteins in st ...
The FTLD risk factor TMEM106B and MAP6 control dendritic
... (Clark & Evans, 1954) confirmed the shift of lysosomes towards the nucleus and the more compact subcellular distribution of lysosomes upon TMEM106B knockdown (Fig 1C). Overexpressing siRNA-resistant human TMEM106B (HA-hT106b*) in cells transfected with TMEM106B siRNA rescued normal lysosomal localiz ...
... (Clark & Evans, 1954) confirmed the shift of lysosomes towards the nucleus and the more compact subcellular distribution of lysosomes upon TMEM106B knockdown (Fig 1C). Overexpressing siRNA-resistant human TMEM106B (HA-hT106b*) in cells transfected with TMEM106B siRNA rescued normal lysosomal localiz ...
Leveau2008 - Johan Leveau
... several species, especially Trichoderma (Steyaert et al., 2003). Mycophagous behaviour of the latter is characterized by: formation of coiled hyphal structures around the hyphae of the host fungus; penetration of the cell wall because of the production of enzymes that break down cell wall components ...
... several species, especially Trichoderma (Steyaert et al., 2003). Mycophagous behaviour of the latter is characterized by: formation of coiled hyphal structures around the hyphae of the host fungus; penetration of the cell wall because of the production of enzymes that break down cell wall components ...
Sears JC and Broihier HT
... regulating cytoskeletal assembly and organization in da neurons (Lefebvre et al., 2015; Santiago and Bashaw, 2014), suggesting that developmental competence for dendrite growth and branching is established by cell-intrinsic factors. Interestingly, a number of transcription factors selectively regula ...
... regulating cytoskeletal assembly and organization in da neurons (Lefebvre et al., 2015; Santiago and Bashaw, 2014), suggesting that developmental competence for dendrite growth and branching is established by cell-intrinsic factors. Interestingly, a number of transcription factors selectively regula ...
Composition and production of thiol constituents induced
... phytochelatins was observed with increased cadmium concentration during the first days of culture. The type of phytochelatins (number of subunits) also was dependent on the concentration of cadmium. The rate of (γ-Glu-Cys)3-Gly accumulation was higher than those of other types of phytochelatins thro ...
... phytochelatins was observed with increased cadmium concentration during the first days of culture. The type of phytochelatins (number of subunits) also was dependent on the concentration of cadmium. The rate of (γ-Glu-Cys)3-Gly accumulation was higher than those of other types of phytochelatins thro ...
Mitochondrial dynamics, mitophagy and cardiovascular disease
... described above (Chen et al. 2011), there is strong evidence that these processes are important in the adult cardiac tissue (Coleman et al. 1987; Delettre et al. 2001; Santel et al. 2003). For example, the proteins involved in fusion and fission are highly expressed in the adult heart (Delettre et a ...
... described above (Chen et al. 2011), there is strong evidence that these processes are important in the adult cardiac tissue (Coleman et al. 1987; Delettre et al. 2001; Santel et al. 2003). For example, the proteins involved in fusion and fission are highly expressed in the adult heart (Delettre et a ...
Autophagy regulation by nutrient signaling
... In order to explain autophagy regulation, we will first describe the autophagy machinery in this section. ATG proteins are often listed in six functional groups that cooperate to perform key processes in autophagosome formation [3]: first, UNC-51-like kinase 1 (ULK1, a yeast Atg1 homolog) kinase com ...
... In order to explain autophagy regulation, we will first describe the autophagy machinery in this section. ATG proteins are often listed in six functional groups that cooperate to perform key processes in autophagosome formation [3]: first, UNC-51-like kinase 1 (ULK1, a yeast Atg1 homolog) kinase com ...
Cell cycle checkpoints in Caenorhabditis elegans
... Figure 24. par-5 is necessary for IR-induced response. .......................................................... 58 Figure 25. par-2, par-3 and mpk-1 are dispensable for the S-phase checkpoint. .................... 60 Figure 26. par-5 is required for replication stress-induced cell cycle delay in t ...
... Figure 24. par-5 is necessary for IR-induced response. .......................................................... 58 Figure 25. par-2, par-3 and mpk-1 are dispensable for the S-phase checkpoint. .................... 60 Figure 26. par-5 is required for replication stress-induced cell cycle delay in t ...
Bile Formation: a Concerted Action of Membrane
... Bile Formation: a Concerted Action of Membrane Transporters in Hepatocytes and Cholangiocytes Akos Zsembery, Theresia Thalhammer, and Jürg Graf A large number of membrane transport mechanisms in hepatocytes and cholangiocytes serves for the secretion of bile acids, various other organic anions, orga ...
... Bile Formation: a Concerted Action of Membrane Transporters in Hepatocytes and Cholangiocytes Akos Zsembery, Theresia Thalhammer, and Jürg Graf A large number of membrane transport mechanisms in hepatocytes and cholangiocytes serves for the secretion of bile acids, various other organic anions, orga ...
Histone methylation
... same histone, but some histone marks are mutually exclusive. For instance, in mammals and yeast, dimethylation of H3R2 (which is established by the methyltransfersase PRMT6 in mammals) is prevented by H3K4me3; conversely, H3R2me2a prevents H3K4 methylation52,53. Similarly, phosphorylation of H3S10 p ...
... same histone, but some histone marks are mutually exclusive. For instance, in mammals and yeast, dimethylation of H3R2 (which is established by the methyltransfersase PRMT6 in mammals) is prevented by H3K4me3; conversely, H3R2me2a prevents H3K4 methylation52,53. Similarly, phosphorylation of H3S10 p ...
The Epigenetic Pathways to Ribosomal DNA Silencing
... In budding yeast, the rDNA region that carries rRNA is organized into a tandem array of 9.1-kb units that are repeated 100 to 200 times on chromosome XII. In particular, the rDNA region is localized to the inner nuclear membrane (INM) of the nucleolus (7, 8). In humans, each rDNA repeat is !43 kb an ...
... In budding yeast, the rDNA region that carries rRNA is organized into a tandem array of 9.1-kb units that are repeated 100 to 200 times on chromosome XII. In particular, the rDNA region is localized to the inner nuclear membrane (INM) of the nucleolus (7, 8). In humans, each rDNA repeat is !43 kb an ...
The Role of Tumor Necrosis Factor-alpha (TNF
... Grounds 1999). Although different cytokines have been implicated in inflammatory myopathies such as polymyositis, dermatomyositis, and inclusion body myositis (Tews and Goebel 1996; Lundberg and Nyberg 1998; De Bleecker et al. 1999), there is little information on changes in the pattern of expressio ...
... Grounds 1999). Although different cytokines have been implicated in inflammatory myopathies such as polymyositis, dermatomyositis, and inclusion body myositis (Tews and Goebel 1996; Lundberg and Nyberg 1998; De Bleecker et al. 1999), there is little information on changes in the pattern of expressio ...
Analysis of Eukaryotic DNA Topoisomerases and
... strand through the transient nick (16). Type II topoisomerases are composed of two identical subunits with relative molecular masses of 170000 or 180000. They require ATP hydrolysis for catalytic activity and can alter DNA topology by creating transient double strand breaks, through which a second i ...
... strand through the transient nick (16). Type II topoisomerases are composed of two identical subunits with relative molecular masses of 170000 or 180000. They require ATP hydrolysis for catalytic activity and can alter DNA topology by creating transient double strand breaks, through which a second i ...