Ammonium toxicity and potassium limitation in yeast.
... We hypothesized that S. cerevisiae could be excreting nitrogencontaining organic compounds in an effort to detoxify ammonium. Accordingly, we used liquid chromatography– tandem mass spectrometry (LC-MS/MS) to test the filtrates of steady-state chemostats for the presence of dozens of different, known ...
... We hypothesized that S. cerevisiae could be excreting nitrogencontaining organic compounds in an effort to detoxify ammonium. Accordingly, we used liquid chromatography– tandem mass spectrometry (LC-MS/MS) to test the filtrates of steady-state chemostats for the presence of dozens of different, known ...
Effect of ciliary neurotrophic factor (CNTF) on motoneuron survival
... identified as the most abundant source of CNTF (Manthorpe et al. 1986) wherefrom it was purified to homogeneity as a basis for microsequencing and finally molecular cloning (Stöckli et al. 1989, 1991). In contrast to nerve growth factor (NGF) and other members ofthe NGF-gene family, i.e. brain-deriv ...
... identified as the most abundant source of CNTF (Manthorpe et al. 1986) wherefrom it was purified to homogeneity as a basis for microsequencing and finally molecular cloning (Stöckli et al. 1989, 1991). In contrast to nerve growth factor (NGF) and other members ofthe NGF-gene family, i.e. brain-deriv ...
Increased Levels of LPS-Binding Protein in Bovine - PubAg
... (MSCC) of <500,000 cells/ml and the absence of detectable bacteria growth from three daily consecutive aseptic milk samples plated on blood agar plates. To assay for MSCC, milk samples were heated to 60°C and subsequently maintained at 40°C until counted on an automated cell counter (Fossomatic mode ...
... (MSCC) of <500,000 cells/ml and the absence of detectable bacteria growth from three daily consecutive aseptic milk samples plated on blood agar plates. To assay for MSCC, milk samples were heated to 60°C and subsequently maintained at 40°C until counted on an automated cell counter (Fossomatic mode ...
Ca signaling and early embryonic patterning during zebrafish
... 4. The potential down-stream targets of these Ca2+ transients are also discussed as well as how they might integrate with other pattern-forming signaling pathways, known to modulate early developmental events. Introduction There is an accumulating body of evidence to suggest that Ca2+ signaling play ...
... 4. The potential down-stream targets of these Ca2+ transients are also discussed as well as how they might integrate with other pattern-forming signaling pathways, known to modulate early developmental events. Introduction There is an accumulating body of evidence to suggest that Ca2+ signaling play ...
A painful TR(i)P to lysosomes
... The functional presence of TRPA1 in the lysosomes of DRG neurons, but not in HEK cells or DRG-derived neuronal cell lines, suggests that TRPA1 is targeted to lysosomes via a mechanism that is specific to primary neurons. A common misconception among channel biologists is that lysosomes are the “dest ...
... The functional presence of TRPA1 in the lysosomes of DRG neurons, but not in HEK cells or DRG-derived neuronal cell lines, suggests that TRPA1 is targeted to lysosomes via a mechanism that is specific to primary neurons. A common misconception among channel biologists is that lysosomes are the “dest ...
A painful TR(i)P to lysosomes
... The functional presence of TRPA1 in the lysosomes of DRG neurons, but not in HEK cells or DRG-derived neuronal cell lines, suggests that TRPA1 is targeted to lysosomes via a mechanism that is specific to primary neurons. A common misconception among channel biologists is that lysosomes are the “dest ...
... The functional presence of TRPA1 in the lysosomes of DRG neurons, but not in HEK cells or DRG-derived neuronal cell lines, suggests that TRPA1 is targeted to lysosomes via a mechanism that is specific to primary neurons. A common misconception among channel biologists is that lysosomes are the “dest ...
Lipid modulation of skeletal muscle mass and function
... Figure 2 Summary of pathways mediating muscle atrophy by saturated fatty acids. Exposure of muscle cells to saturated fatty acids such as palmitate (C16:0) results in the intracellular accumulation of toxic lipid intermediates such as ceramide and diacylglycerol. (A) Increased ceramide levels can le ...
... Figure 2 Summary of pathways mediating muscle atrophy by saturated fatty acids. Exposure of muscle cells to saturated fatty acids such as palmitate (C16:0) results in the intracellular accumulation of toxic lipid intermediates such as ceramide and diacylglycerol. (A) Increased ceramide levels can le ...
Prox1 and fibroblast growth factor receptors form a
... surrounding WT lenses had few or no TUNEL-positive nuclei at E13.5 (Fig. 2E,E″), whereas Prox1 cKO eyes exhibited apoptosis of the lens vasculature at E13.5 (Fig. 2F,F′), leading to its loss by E15.5 (Fig. 1F). Prox1 cKO mice have reduced expression of LF cell markers ...
... surrounding WT lenses had few or no TUNEL-positive nuclei at E13.5 (Fig. 2E,E″), whereas Prox1 cKO eyes exhibited apoptosis of the lens vasculature at E13.5 (Fig. 2F,F′), leading to its loss by E15.5 (Fig. 1F). Prox1 cKO mice have reduced expression of LF cell markers ...
MM2009_Zhang - Australian Institute for Bioengineering and
... antisense 3’-ctCCCGAUUUCAUAUAGGUAA -5’ These simulations are closely related to the nano-neuro initiative “Novel hybrid inorganic nano-particles for effective siRNA delivery to neurons” between QBI and AIBN . MM 2009 ...
... antisense 3’-ctCCCGAUUUCAUAUAGGUAA -5’ These simulations are closely related to the nano-neuro initiative “Novel hybrid inorganic nano-particles for effective siRNA delivery to neurons” between QBI and AIBN . MM 2009 ...
A Study of Specificity of Cores for Group D Streptococci
... protrusions from the main cell body (Cohen et al. 1968); in contrast, in the micrographs of Corfield & Smith (1968), intracellular 'microtubular ' structures are hollow tubular invaginations of the cytoplasmic membrane of not more than about 0 - 2pm. in length and can be very numerous in some cells. ...
... protrusions from the main cell body (Cohen et al. 1968); in contrast, in the micrographs of Corfield & Smith (1968), intracellular 'microtubular ' structures are hollow tubular invaginations of the cytoplasmic membrane of not more than about 0 - 2pm. in length and can be very numerous in some cells. ...
Mathematical Biology
... a whole is treadmilling) it is reasonable to suppose that the probability of cutting increases along the length of the filament from its barbed end. This type of assumption will be incorporated into our model. We note, however, that there is some controversy still about whether filaments do treadmil ...
... a whole is treadmilling) it is reasonable to suppose that the probability of cutting increases along the length of the filament from its barbed end. This type of assumption will be incorporated into our model. We note, however, that there is some controversy still about whether filaments do treadmil ...
Endoplasmic Reticulum-Associated Degradation of Ricin A Chain
... two mutants were pulse labeled and chased for different times in the absence or in the presence of b-lactone. Protoplasts were then fractionated into cytosolic (S, soluble) and microsomal (P, pellet) fractions (Fig. 3). In the absence of b-lactone, a significant amount of RTA* and RTA*0K was recover ...
... two mutants were pulse labeled and chased for different times in the absence or in the presence of b-lactone. Protoplasts were then fractionated into cytosolic (S, soluble) and microsomal (P, pellet) fractions (Fig. 3). In the absence of b-lactone, a significant amount of RTA* and RTA*0K was recover ...
Nuclear Factor-κB Modulates the p53 Response in Neurons
... Previous studies have shown that DNA damage-evoked death of primary cortical neurons occurs in a p53 and cyclin-dependent kinasedependent (CDK) manner. The manner by which these signals modulate death is unclear. Nuclear factor-B (NF-B) is a group of transcription factors that potentially interact ...
... Previous studies have shown that DNA damage-evoked death of primary cortical neurons occurs in a p53 and cyclin-dependent kinasedependent (CDK) manner. The manner by which these signals modulate death is unclear. Nuclear factor-B (NF-B) is a group of transcription factors that potentially interact ...
Inhibitory Regulation of Higher-Plant Myosin by
... protein kinase, which is inactivated by high Ca21. The inhibitory effect of Ca21 on cytoplasmic streaming has also been reported in some higher-plant cells such as lily (Lilium longiflorum) pollen tubes (Kohno and Shimmen, 1988b), stamen hair cells of Tradescantia (Doree and Picard, 1980), trichome ...
... protein kinase, which is inactivated by high Ca21. The inhibitory effect of Ca21 on cytoplasmic streaming has also been reported in some higher-plant cells such as lily (Lilium longiflorum) pollen tubes (Kohno and Shimmen, 1988b), stamen hair cells of Tradescantia (Doree and Picard, 1980), trichome ...
Full-Text PDF
... yeast [31]. The interaction of CL with Lon protease, which is involved in degrading misfolded proteins [32,33] influences the action of Lon by inhibiting its proteolytic and ATPase activities [34]. In prokaryotes, the role of acidic phospholipids also appears to be linked to chromosomal and cell div ...
... yeast [31]. The interaction of CL with Lon protease, which is involved in degrading misfolded proteins [32,33] influences the action of Lon by inhibiting its proteolytic and ATPase activities [34]. In prokaryotes, the role of acidic phospholipids also appears to be linked to chromosomal and cell div ...
original version
... yeast [31]. The interaction of CL with Lon protease, which is involved in degrading misfolded proteins [32,33] influences the action of Lon by inhibiting its proteolytic and ATPase activities [34]. In prokaryotes, the role of acidic phospholipids also appears to be linked to chromosomal and cell div ...
... yeast [31]. The interaction of CL with Lon protease, which is involved in degrading misfolded proteins [32,33] influences the action of Lon by inhibiting its proteolytic and ATPase activities [34]. In prokaryotes, the role of acidic phospholipids also appears to be linked to chromosomal and cell div ...
View PDF - OMICS International
... tuberculosis [15]. It has been validated in the M. tuberculosis, if the organisms are physiologically inactive for long time period; its storage of sugars becomes very important for survival. Various groups of scientific community has been reported that, glycan’s may regulate biochemical pathways by ...
... tuberculosis [15]. It has been validated in the M. tuberculosis, if the organisms are physiologically inactive for long time period; its storage of sugars becomes very important for survival. Various groups of scientific community has been reported that, glycan’s may regulate biochemical pathways by ...
Spatial localization of the first and last enzymes effectively connects
... suggests that enzymes could, not only freely diffuse, but also show spatial ordering in the cytoplasm. To explore the rules of putative enzymatic organization, we took advantage of the images of A complete Set of E.coli K-12 ORF Archive (ASKA) library of GFP-tagged ORF clones [18] displayed on the G ...
... suggests that enzymes could, not only freely diffuse, but also show spatial ordering in the cytoplasm. To explore the rules of putative enzymatic organization, we took advantage of the images of A complete Set of E.coli K-12 ORF Archive (ASKA) library of GFP-tagged ORF clones [18] displayed on the G ...
Mechanisms of plasmid stable maintenance with special
... the probability of receiving a plasmid by a dividing cell there are very special strategies adopted by plasmids preventing plasmid-free segregants from surviving. The idea of an “addiction” mechanism leading to very efficient plasmid maintenance comes from Koyama (Koyama et al., 1975). In his consid ...
... the probability of receiving a plasmid by a dividing cell there are very special strategies adopted by plasmids preventing plasmid-free segregants from surviving. The idea of an “addiction” mechanism leading to very efficient plasmid maintenance comes from Koyama (Koyama et al., 1975). In his consid ...
PureYield™ RNA Midiprep System Technical Manual
... isolation methods yield RNA that is contaminated with some genomic DNA unless DNase treatment is performed. This contamination can interfere with sensitive methods, such as RT-PCR, real-time qRT-PCR and microarray analysis. DNase treatment can be used to reduce or eliminate contaminating DNA from pu ...
... isolation methods yield RNA that is contaminated with some genomic DNA unless DNase treatment is performed. This contamination can interfere with sensitive methods, such as RT-PCR, real-time qRT-PCR and microarray analysis. DNase treatment can be used to reduce or eliminate contaminating DNA from pu ...
Suppressor of Hairless-independent events in
... flies and the phenotypes were compared to those induced by the expression of activation forms of Notch (Fig. 1). The ankyrin repeats elicit distinct mutant phenotypes The expression of the entire intracellular domain of Notch under the sev promoter (sev-Nact and sev-Nnucl in Fig. 1) in the developin ...
... flies and the phenotypes were compared to those induced by the expression of activation forms of Notch (Fig. 1). The ankyrin repeats elicit distinct mutant phenotypes The expression of the entire intracellular domain of Notch under the sev promoter (sev-Nact and sev-Nnucl in Fig. 1) in the developin ...
Modeled Osteopathic Manipulative Treatments: A Review of Their in
... acyclic long duration strain refer to the same technique; ...
... acyclic long duration strain refer to the same technique; ...
A. Work Accomplished by You and/or Others Cellular biology of
... functional form (reviewed in Buchner, 1996). For many proteins, it is a “cradle to grave” interaction, with chaperones binding early upon emergence from the ribosome (Pfund et al., 1998), maintenance of a persistent substrate-chaperone heterocomplex (Pratt and Toft, 1997), and in some cases directed ...
... functional form (reviewed in Buchner, 1996). For many proteins, it is a “cradle to grave” interaction, with chaperones binding early upon emergence from the ribosome (Pfund et al., 1998), maintenance of a persistent substrate-chaperone heterocomplex (Pratt and Toft, 1997), and in some cases directed ...