CHARACTERIZATION OF MOLECULES SHOWING
... PRINS functions as a noncoding regulatory RNA, playing a protective role in cells exposed to stress. It is described that long mRNA-like ncRNAs form complexes with proteins, and act as regulators of various cellular functions. Therefore we have performed an in vitro binding assay which revealed that ...
... PRINS functions as a noncoding regulatory RNA, playing a protective role in cells exposed to stress. It is described that long mRNA-like ncRNAs form complexes with proteins, and act as regulators of various cellular functions. Therefore we have performed an in vitro binding assay which revealed that ...
Glial Cell Inhibition of Neurons by Release of ATP
... the Lucifer yellow-filled cell was viewed with confocal microscopy using 458 nm excitation and a 500 nm long-pass barrier filter. The depth of the deepest dendrites of a neuron was determined from confocal fluorescence and IR-DIC observations made on the live retina immediately after recording from ...
... the Lucifer yellow-filled cell was viewed with confocal microscopy using 458 nm excitation and a 500 nm long-pass barrier filter. The depth of the deepest dendrites of a neuron was determined from confocal fluorescence and IR-DIC observations made on the live retina immediately after recording from ...
Some Recent Work on the Structure of the Plant Cell Wall
... is itself transformed into cell wall material (1). Grafe and his co-workers, in a series of papers (13, 14, 15), have confirmed Hansteen-Cranner's work and have carried out extensive investigations upon the chemistry of these phosphatides. From this work it becomes clear that these phosphatides have ...
... is itself transformed into cell wall material (1). Grafe and his co-workers, in a series of papers (13, 14, 15), have confirmed Hansteen-Cranner's work and have carried out extensive investigations upon the chemistry of these phosphatides. From this work it becomes clear that these phosphatides have ...
Some Recent Work on the Structure of the Plant Cell Wall
... is itself transformed into cell wall material (1). Grafe and his co-workers, in a series of papers (13, 14, 15), have confirmed Hansteen-Cranner's work and have carried out extensive investigations upon the chemistry of these phosphatides. From this work it becomes clear that these phosphatides have ...
... is itself transformed into cell wall material (1). Grafe and his co-workers, in a series of papers (13, 14, 15), have confirmed Hansteen-Cranner's work and have carried out extensive investigations upon the chemistry of these phosphatides. From this work it becomes clear that these phosphatides have ...
Some Recent Work on the Structure of the Plant Cell Wall
... is itself transformed into cell wall material (1). Grafe and his co-workers, in a series of papers (13, 14, 15), have confirmed Hansteen-Cranner's work and have carried out extensive investigations upon the chemistry of these phosphatides. From this work it becomes clear that these phosphatides have ...
... is itself transformed into cell wall material (1). Grafe and his co-workers, in a series of papers (13, 14, 15), have confirmed Hansteen-Cranner's work and have carried out extensive investigations upon the chemistry of these phosphatides. From this work it becomes clear that these phosphatides have ...
Protoplast Culture: definition
... Once the protoplasts have regenerated a cell wall, they undergo cell division and form a callus.This callus can be subcultured. The callus may undergo embryogenesis or organogenesis after about 3-4 weeks, in the correct culture conditions. The embryoids/organs can be grown up in the same manner as f ...
... Once the protoplasts have regenerated a cell wall, they undergo cell division and form a callus.This callus can be subcultured. The callus may undergo embryogenesis or organogenesis after about 3-4 weeks, in the correct culture conditions. The embryoids/organs can be grown up in the same manner as f ...
The Putative Arabidopsis Arp2/3 Complex
... eukaryotes, but its biological functions are not well understood in multicellular organisms. The model plant Arabidopsis provides many advantages for genetic dissection of the function of this conserved actin-nucleating machinery, yet the existence of this complex in plants has not been determined. ...
... eukaryotes, but its biological functions are not well understood in multicellular organisms. The model plant Arabidopsis provides many advantages for genetic dissection of the function of this conserved actin-nucleating machinery, yet the existence of this complex in plants has not been determined. ...
Liver development
... the hepatic diverticulum gives rise to the liver and intrahepatic biliary tree, while the posterior portion forms the gall bladder and extrahepatic bile ducts. At e9.5, the hepatic endoderm cells, known as hepatoblasts delaminate from the epithelium and invade the adjacent septum transversum mesench ...
... the hepatic diverticulum gives rise to the liver and intrahepatic biliary tree, while the posterior portion forms the gall bladder and extrahepatic bile ducts. At e9.5, the hepatic endoderm cells, known as hepatoblasts delaminate from the epithelium and invade the adjacent septum transversum mesench ...
Epiblast and primitive-streak origins of the endoderm in
... The present study had three aims. First, we determined the primitive-streak origin of the endoderm using supravital fluorescent markers, and followed the movement of the prospective endodermal cells as they dispersed to generate the definitive endodermal layer. The results demonstrate that between s ...
... The present study had three aims. First, we determined the primitive-streak origin of the endoderm using supravital fluorescent markers, and followed the movement of the prospective endodermal cells as they dispersed to generate the definitive endodermal layer. The results demonstrate that between s ...
Pausing of Golgi Bodies on Microtubules Regulates
... images (Figures 1P and 1Q). In addition to Golgi bodies, we identified a second, relatively rare population (<1 observed per cell per 10 min imaging period) of smaller, homogeneously labeled particles with the same rapid, nonlinear movement as Golgi bodies (Figures 1J and 1K). These particles were i ...
... images (Figures 1P and 1Q). In addition to Golgi bodies, we identified a second, relatively rare population (<1 observed per cell per 10 min imaging period) of smaller, homogeneously labeled particles with the same rapid, nonlinear movement as Golgi bodies (Figures 1J and 1K). These particles were i ...
structural and ultrastructural data on side effects of cisplatin in
... lymphotoxic effects, as cyclophosphamide, cisplatin, vincristin, carboplatin etc., can induce several side effects on the immune system (Goodman et al, 1990; Kelly and Rosencweig, 1990; Yoshiruni et al., 1990; Blank et al., 1991; Luster et al., 1993; Milicevic et al., 1994; Crăciunaş et al., 1995; C ...
... lymphotoxic effects, as cyclophosphamide, cisplatin, vincristin, carboplatin etc., can induce several side effects on the immune system (Goodman et al, 1990; Kelly and Rosencweig, 1990; Yoshiruni et al., 1990; Blank et al., 1991; Luster et al., 1993; Milicevic et al., 1994; Crăciunaş et al., 1995; C ...
RETINOBLASTOMA RELATED1 Regulates
... divisions in the stomata lineage. This cell lineage produces stomata, gas exchange pores formed by two guard cells, as well as epidermal pavement cells (Bergmann and Sack, 2007). Stomata are produced via a series of asymmetric divisions, the first of which occurs in a parent meristemoid mother cell t ...
... divisions in the stomata lineage. This cell lineage produces stomata, gas exchange pores formed by two guard cells, as well as epidermal pavement cells (Bergmann and Sack, 2007). Stomata are produced via a series of asymmetric divisions, the first of which occurs in a parent meristemoid mother cell t ...
Coupling cellular oscillators—circadian and cell division cycles in cyanobacteria Bernardo F Pando
... cycles of light and dark and the propagation of such synchronization for several days after transitioning to constant light environments. In individual mouse fibroblasts, Nagoshi et al. [34] investigated whether circadian oscillations were maintained after detachment from circadian pacemakers in the ...
... cycles of light and dark and the propagation of such synchronization for several days after transitioning to constant light environments. In individual mouse fibroblasts, Nagoshi et al. [34] investigated whether circadian oscillations were maintained after detachment from circadian pacemakers in the ...
Feasibility of using GFP‐expressing Escherichia coli, coupled with
... Each prey type was inoculated into six £asks containing 60 ml Chalkley's medium, to give an initial concentration of 5U107 particles ml31 , in excess of indigenous bacteria (1.25U106 cells ml31 ). All £asks were incubated at 20³C and the RF ml31 and prey concentration in each £ask were determined ov ...
... Each prey type was inoculated into six £asks containing 60 ml Chalkley's medium, to give an initial concentration of 5U107 particles ml31 , in excess of indigenous bacteria (1.25U106 cells ml31 ). All £asks were incubated at 20³C and the RF ml31 and prey concentration in each £ask were determined ov ...
In-vivo detection of binary PKA network interactions upon activation
... differentially localized phosphotransferase inhibitors, such as RIa and PKI (Fig. 2B). GPCR-controlled cAMP-mobilization is sensed by differentially localized R subunits bound to PKAc. cAMP-binding to R leads to dissociation and activation of PKAc12. In addition to cAMP-degradation and re-associati ...
... differentially localized phosphotransferase inhibitors, such as RIa and PKI (Fig. 2B). GPCR-controlled cAMP-mobilization is sensed by differentially localized R subunits bound to PKAc. cAMP-binding to R leads to dissociation and activation of PKAc12. In addition to cAMP-degradation and re-associati ...
The putative phosphatase All1758 is necessary for normal growth
... et al., 2009). The protein kinases Pkn41 and Pkn42, which contain serine/threonine kinase and histidine kinase domains, respectively, are cotranscribed specifically under iron-deficient conditions and regulated by NtcA (Cheng et al., 2006). The nitrogen regulatory protein PII encoded by glnB is diff ...
... et al., 2009). The protein kinases Pkn41 and Pkn42, which contain serine/threonine kinase and histidine kinase domains, respectively, are cotranscribed specifically under iron-deficient conditions and regulated by NtcA (Cheng et al., 2006). The nitrogen regulatory protein PII encoded by glnB is diff ...
Small Signaling Peptides in Arabidopsis Development
... proliferation of cells in low-density plant cell culture, but it was unclear which unknown substance(s) within the extracts was active in promoting the proliferation. A bioassay based on the mitotic activity of asparagus (Asparagus officinalis) mesophyll cells in liquid culture identified an active, g ...
... proliferation of cells in low-density plant cell culture, but it was unclear which unknown substance(s) within the extracts was active in promoting the proliferation. A bioassay based on the mitotic activity of asparagus (Asparagus officinalis) mesophyll cells in liquid culture identified an active, g ...
Circulating Tumor Cells: A Window Into Tumor Development and
... enabling single CTC genomic analysis. Two major disApril 2015, Vol. 22, No. 2 ...
... enabling single CTC genomic analysis. Two major disApril 2015, Vol. 22, No. 2 ...
Metabolic allometric scaling model. Combining cellular
... processing of nutrients. The rate of energy production is called metabolic rate. Metabolism is very important from evolutionary, ecological perspectives, and for organisms' development and functioning. It depends on different parameters, of which organisms' mass is considered as one of the most impo ...
... processing of nutrients. The rate of energy production is called metabolic rate. Metabolism is very important from evolutionary, ecological perspectives, and for organisms' development and functioning. It depends on different parameters, of which organisms' mass is considered as one of the most impo ...
Minimally invasive determination of mRNA
... culture was used for all different aTc induction levels, by growing distinct cultures. A 0.3 ml drop of cell culture was placed on a glass cover slip then covered with a 3% low melting-point agarose LB gel containing equal concentrations of aTc as the liquid culture. The gel padding did not contain a ...
... culture was used for all different aTc induction levels, by growing distinct cultures. A 0.3 ml drop of cell culture was placed on a glass cover slip then covered with a 3% low melting-point agarose LB gel containing equal concentrations of aTc as the liquid culture. The gel padding did not contain a ...
Mitochondrion and Chloroplast Regulation of Plant Programmed
... the fact that dissociation of hexokinase from animal mitochondria in the presence of apoptotic stimuli but in the absence of Bax and Bak still results in cyt c release (though not as effective) which is not suppressed by Bcl-2 [72]. It is possible that while both animal and plant cells employ hexoki ...
... the fact that dissociation of hexokinase from animal mitochondria in the presence of apoptotic stimuli but in the absence of Bax and Bak still results in cyt c release (though not as effective) which is not suppressed by Bcl-2 [72]. It is possible that while both animal and plant cells employ hexoki ...
Expanded GAA repeats impair FXN gene expression and reposition
... Figure 1. The expanded GAA repeat FXN transgene associates with the NL more frequently in an FXN-GAA-MS2-Luc cell model. (A) Schematic representation of the pBACFXN-MS2-Luc and pBAC-FXN-GAA-MS2-Luc vectors. Each vector carries either six or ∼310 GAA repeats in intron 1, an array of 24 MBS in exon 2 ...
... Figure 1. The expanded GAA repeat FXN transgene associates with the NL more frequently in an FXN-GAA-MS2-Luc cell model. (A) Schematic representation of the pBACFXN-MS2-Luc and pBAC-FXN-GAA-MS2-Luc vectors. Each vector carries either six or ∼310 GAA repeats in intron 1, an array of 24 MBS in exon 2 ...
silicone rubber in a parallel plate flow chamber in the
... Fig. 1 shows the growth curves of P. aeruginosa AK1 cells suspended in PBS supplemented with various amounts of nutrient broth. As can be seen, growth is virtually absent when less than 2 % broth is added, while for higher concentrations of broth significant growth occurs. Therefore, minimal growth ...
... Fig. 1 shows the growth curves of P. aeruginosa AK1 cells suspended in PBS supplemented with various amounts of nutrient broth. As can be seen, growth is virtually absent when less than 2 % broth is added, while for higher concentrations of broth significant growth occurs. Therefore, minimal growth ...
Mypt1-mediated spatial positioning of Bmp2
... the expression of these genes and found that both prox1 and hhex displayed similar expression patterns in the liver primordia of WT and sq181 embryos at 30 hpf (Fig. 2). After this time, however, their expression in WT and mutant embryos diverged. Whereas the hhexand prox1-positive cells in WT embry ...
... the expression of these genes and found that both prox1 and hhex displayed similar expression patterns in the liver primordia of WT and sq181 embryos at 30 hpf (Fig. 2). After this time, however, their expression in WT and mutant embryos diverged. Whereas the hhexand prox1-positive cells in WT embry ...