Title The molecular architecture of the plant nuclear
... that the bulk of the NPC structures evolved through a series of gene duplications and ...
... that the bulk of the NPC structures evolved through a series of gene duplications and ...
Phosphorylation-Dependent Regulation of Septin
... Cdc12, and Shs1/Sep7, were investigated. Each septin was tagged individually at its N terminus with GFP and expressed under the control of its own promoter. Half of the fluorescently labeled septin ring was irradiated with a laser beam to irreversibly bleach GFP. Fluorescence was then monitored over ...
... Cdc12, and Shs1/Sep7, were investigated. Each septin was tagged individually at its N terminus with GFP and expressed under the control of its own promoter. Half of the fluorescently labeled septin ring was irradiated with a laser beam to irreversibly bleach GFP. Fluorescence was then monitored over ...
Corresponding author: Dr. A. Lacey Samuels
... breakdown products were consistently shown to be radioactive, indicating incorporation of 3H-phenylalanine into the lignin polymer (Supplemental Figure 1). To investigate the nature of the soluble metabolites that were labeled during the four hour incubation with 3H-phenylalanine, methanol soluble p ...
... breakdown products were consistently shown to be radioactive, indicating incorporation of 3H-phenylalanine into the lignin polymer (Supplemental Figure 1). To investigate the nature of the soluble metabolites that were labeled during the four hour incubation with 3H-phenylalanine, methanol soluble p ...
- Wiley Online Library
... positioning. Efficient functioning of both processes depends on the ability of these two proteins to recruit other proteins involved in the each stage (Mattei et al., 2010). Using fluorescently labeled vancomycin, an antibiotic that binds to peptidoglycan, cell wall elongation in B. subtilis was sho ...
... positioning. Efficient functioning of both processes depends on the ability of these two proteins to recruit other proteins involved in the each stage (Mattei et al., 2010). Using fluorescently labeled vancomycin, an antibiotic that binds to peptidoglycan, cell wall elongation in B. subtilis was sho ...
The control of sexual identity in the Drosophila
... promote female germ cell development in other assays (Hinson and Nagoshi, 1999; Steinmann-Zwicky et al., 1989). However, gain of Sxl function in XY germ cells does not interfere with male development, as it does in the soma, indicating that Sxl is not sufficient to activate female germ cell developm ...
... promote female germ cell development in other assays (Hinson and Nagoshi, 1999; Steinmann-Zwicky et al., 1989). However, gain of Sxl function in XY germ cells does not interfere with male development, as it does in the soma, indicating that Sxl is not sufficient to activate female germ cell developm ...
pdf, 1.5 MB - The Nebenführ Lab
... Some a-1,2 mannosidases are localized to the Golgi (Herscovics et al., 1994; Lal et al., 1994), although other members of the family were found in the ER (e.g. Roth et al., 1990; Burke et al., 1996). The localization of GmMan1 was tested by creating an in-frame fusion to a modified GFP (GmMan1::GFP) ...
... Some a-1,2 mannosidases are localized to the Golgi (Herscovics et al., 1994; Lal et al., 1994), although other members of the family were found in the ER (e.g. Roth et al., 1990; Burke et al., 1996). The localization of GmMan1 was tested by creating an in-frame fusion to a modified GFP (GmMan1::GFP) ...
phosphatases and differentiation of the golgi apparatus
... zone' (Clowes, 1961), mitotic activity is apparently much lower than in some of the other cell types of the root, as are certain other metabolic activities (Jensen, 1957, 1958). Just basipetal to this region the cells, here termed apical initials, have a higher rate of mitotic activity, but are not ...
... zone' (Clowes, 1961), mitotic activity is apparently much lower than in some of the other cell types of the root, as are certain other metabolic activities (Jensen, 1957, 1958). Just basipetal to this region the cells, here termed apical initials, have a higher rate of mitotic activity, but are not ...
gland - Sinoe Medical Association
... Exocrine Glands – Glands that secrete their products onto the apical (or epithelia) surface directly OR via epithelial ducts or tubes that are connected to the apical surface. These exocrine glands are composed of highly specialized epithelial cells and thus are classified as glandular epithelia. En ...
... Exocrine Glands – Glands that secrete their products onto the apical (or epithelia) surface directly OR via epithelial ducts or tubes that are connected to the apical surface. These exocrine glands are composed of highly specialized epithelial cells and thus are classified as glandular epithelia. En ...
Full Text
... function in Japan. Some cell growth factors require binding to heparan sulfate for the expression of their activities. Kimata and colleagues found that basic fibroblast growth factor binds specifically to a triple repeat of GlcNSO3-IdoA(2SO4) units (Habuchi et al., 1992), and hepatocyte growth facto ...
... function in Japan. Some cell growth factors require binding to heparan sulfate for the expression of their activities. Kimata and colleagues found that basic fibroblast growth factor binds specifically to a triple repeat of GlcNSO3-IdoA(2SO4) units (Habuchi et al., 1992), and hepatocyte growth facto ...
27-Bacteria and Archaea
... pink (Figure 27.1), a sign of waters so salty that they would dehydrate your skin if you took a dip. The salt concentration can reach 32%, nearly ten times that of seawater. Yet despite these harsh conditions, the dramatic color of these waters is caused not by minerals or other nonliving sources, b ...
... pink (Figure 27.1), a sign of waters so salty that they would dehydrate your skin if you took a dip. The salt concentration can reach 32%, nearly ten times that of seawater. Yet despite these harsh conditions, the dramatic color of these waters is caused not by minerals or other nonliving sources, b ...
Cpf1 Is a Single RNA-Guided Endonuclease of a
... targeted (Figure 1E), suggesting that the middle T is more critical for PAM recognition than the first T and that, in agreement with the sequence motifs depleted in the PAM discovery assay (Figure S1D), the PAM might be more relaxed than 50 -TTN. The Cpf1-Associated CRISPR Array Is Processed Indepen ...
... targeted (Figure 1E), suggesting that the middle T is more critical for PAM recognition than the first T and that, in agreement with the sequence motifs depleted in the PAM discovery assay (Figure S1D), the PAM might be more relaxed than 50 -TTN. The Cpf1-Associated CRISPR Array Is Processed Indepen ...
Calcium-Containing Organelles Display Unique Reactivity to
... 300 msec pulse, n 5 11 cells) caused a transient rise in [Ca]i, which initially started in CCOs (see the insert amplification of the first part of the graph) and was larger in the nucleus than in cytoplasm or the CCOs, and a slower recovery to baseline in the latter than in former two compartments. ...
... 300 msec pulse, n 5 11 cells) caused a transient rise in [Ca]i, which initially started in CCOs (see the insert amplification of the first part of the graph) and was larger in the nucleus than in cytoplasm or the CCOs, and a slower recovery to baseline in the latter than in former two compartments. ...
Cagnac, O., Leterrier, M., Yeager, M. and Blumwald, E. (2007).
... Two other Nha1p homologues, Kha1p and Nhx1p, have been identified in S. cerevisiae. Kha1p has been described as a putative K⫹/H⫹ antiporter, and its deletion induced a growth defect at high external pH and hygromycin (10). Kha1p co-localized with Mntp1p, a Golgi-specific marker (11). Although Kha1p ...
... Two other Nha1p homologues, Kha1p and Nhx1p, have been identified in S. cerevisiae. Kha1p has been described as a putative K⫹/H⫹ antiporter, and its deletion induced a growth defect at high external pH and hygromycin (10). Kha1p co-localized with Mntp1p, a Golgi-specific marker (11). Although Kha1p ...
Archenteron precursor cells can organize secondary axial structures
... gut tissue or (3) a combination of such signals could be involved (these possibilities are summarized in Fig. 1B). In order to provide experimental support for one of these alternative mechanisms, we have used a chimeric embryo approach to implant gut progenitors in ectopic locations to assess their ...
... gut tissue or (3) a combination of such signals could be involved (these possibilities are summarized in Fig. 1B). In order to provide experimental support for one of these alternative mechanisms, we have used a chimeric embryo approach to implant gut progenitors in ectopic locations to assess their ...
Group-specific effects on coastal bacterioplankton of
... general response patterns could be found for the dominant marine bacterioplankton groups (Alpha- and Gammaproteobacteria or Bacteroidetes), since different species within the same broad phylogenetic groups reacted differently to the PUAs. The enzymatic cascade leading to PUA production has been show ...
... general response patterns could be found for the dominant marine bacterioplankton groups (Alpha- and Gammaproteobacteria or Bacteroidetes), since different species within the same broad phylogenetic groups reacted differently to the PUAs. The enzymatic cascade leading to PUA production has been show ...
Cyclic AMP-Mediated Inhibition of Cell Growth Requires the Small G
... Cell culturing conditions and treatments. Hek293 and NIH 3T3 cells were cultured in Dulbecco modified Eagle medium (DMEM) plus 10% fetal calf serum at 37°C in 5% CO2. Cells were maintained in serum-free DMEM for 16 h at 37°C in 5% CO2 prior to treatment with various reagents for coimmunoprecipitatio ...
... Cell culturing conditions and treatments. Hek293 and NIH 3T3 cells were cultured in Dulbecco modified Eagle medium (DMEM) plus 10% fetal calf serum at 37°C in 5% CO2. Cells were maintained in serum-free DMEM for 16 h at 37°C in 5% CO2 prior to treatment with various reagents for coimmunoprecipitatio ...
Subset-Specific Reductions in Lung Lymphocyte
... Following Intratracheal Antigen Challenge in Endothelial Selectin-Deficient Mice1,2 Jeffrey L. Curtis,3,4*‡§储 Joanne Sonstein,* Ronald A. Craig,† Jill C. Todt,* Randall N. Knibbs,† Timothy Polak,* Daniel C. Bullard,¶ and Lloyd M. Stoolman3†‡§ We previously demonstrated induction and expression of CD ...
... Following Intratracheal Antigen Challenge in Endothelial Selectin-Deficient Mice1,2 Jeffrey L. Curtis,3,4*‡§储 Joanne Sonstein,* Ronald A. Craig,† Jill C. Todt,* Randall N. Knibbs,† Timothy Polak,* Daniel C. Bullard,¶ and Lloyd M. Stoolman3†‡§ We previously demonstrated induction and expression of CD ...
Cell Communication and Signaling
... Food-borne infections with pathogenic bacteria represent one of the leading causes of morbidity and death in humans. Estimations by the World Health Organization WHO suggest that the human population worldwide suffers from about 4.5 billion incidences of diarrhoea every year, causing approximately 1 ...
... Food-borne infections with pathogenic bacteria represent one of the leading causes of morbidity and death in humans. Estimations by the World Health Organization WHO suggest that the human population worldwide suffers from about 4.5 billion incidences of diarrhoea every year, causing approximately 1 ...
zjawisko oddzia*ywania allelopatycznego sinic i glonów w
... submarina compared to control cultures that grew on mineral medium f/2. Similar results were presented by Valdor & Aboal (2007), where morphological changes under the influence of an extract of cyanobacteria Geitlerinema sp., Oscillatoria sp. and Phormidium sp. were observed under light and electron ...
... submarina compared to control cultures that grew on mineral medium f/2. Similar results were presented by Valdor & Aboal (2007), where morphological changes under the influence of an extract of cyanobacteria Geitlerinema sp., Oscillatoria sp. and Phormidium sp. were observed under light and electron ...
The Nuclear Compartment Commonality Hypothesis, Enucleation
... Page 2 of 5 independent studies of cell fine structure, cell composition and phylogeny. The most likely candidate for the Bacteria is an early member of the PVC Superphylum (the Planctomycetes – Verrucomicrobia – Chlamydia phyla) of the Bacteria, which also includes several other phyla (Poribacter, ...
... Page 2 of 5 independent studies of cell fine structure, cell composition and phylogeny. The most likely candidate for the Bacteria is an early member of the PVC Superphylum (the Planctomycetes – Verrucomicrobia – Chlamydia phyla) of the Bacteria, which also includes several other phyla (Poribacter, ...
article in press - MPI
... The first morphological sign of vertebrate postcranial body segmentation is the sequential production from posterior paraxial mesoderm of blocks of cells termed somites. Each of these embryonic structures is polarized along the anterior/posterior axis, a subdivision first distinguished by marker gen ...
... The first morphological sign of vertebrate postcranial body segmentation is the sequential production from posterior paraxial mesoderm of blocks of cells termed somites. Each of these embryonic structures is polarized along the anterior/posterior axis, a subdivision first distinguished by marker gen ...
Hartman Amy Hartman Dr. Bert Ely Biol 303 1 November 2012 Effect
... cancer of the esophagus (Anand and Weinstein 2012). In order to prevent the development of such debilitating diseases, scientists are looking into several different types of genetic causes for affected tissue for diseases like colorectal cancer and Barrett’s Metaplasia. Both of the experiments pursu ...
... cancer of the esophagus (Anand and Weinstein 2012). In order to prevent the development of such debilitating diseases, scientists are looking into several different types of genetic causes for affected tissue for diseases like colorectal cancer and Barrett’s Metaplasia. Both of the experiments pursu ...
University of Groningen Carbohydrate production by
... Alternatively, there may be a different type of glucan produced by colonial Phaeocystis cells. If there is another type of storage glucan besides chrysolaminaran, it is not clear if this is located in the cells or in the colony matrix. In all plants photosynthetic rates exceed metabolic demands when ...
... Alternatively, there may be a different type of glucan produced by colonial Phaeocystis cells. If there is another type of storage glucan besides chrysolaminaran, it is not clear if this is located in the cells or in the colony matrix. In all plants photosynthetic rates exceed metabolic demands when ...
The different shapes of cocci
... postfissional movement of the cells, leading to the formation of irregular clusters (Koyama et al., 1977). Despite the fact that these observations were made three decades ago, the mechanism that determines the precise placement of the septum in alternating perpendicular planes is far from understoo ...
... postfissional movement of the cells, leading to the formation of irregular clusters (Koyama et al., 1977). Despite the fact that these observations were made three decades ago, the mechanism that determines the precise placement of the septum in alternating perpendicular planes is far from understoo ...