In Search of Mitochondrial Mechanisms: Interfield
... produced a reaction. Or once a reaction had been achieved, they might add a poison and determine which chemical product accumulated. These methods were highly successful over time in isolating enzymes that catalyzed reactions, including many of those involved in cellular respiration. However, it had ...
... produced a reaction. Or once a reaction had been achieved, they might add a poison and determine which chemical product accumulated. These methods were highly successful over time in isolating enzymes that catalyzed reactions, including many of those involved in cellular respiration. However, it had ...
A Simple 1-D Physical Model for the Crawling Nematode Sperm Cell
... File: KAPP/822-joss/110_3-6 457171(21p) - Page : 1/21 - Op: SD - Time: 07:12 - Date: 18:11:2002 ...
... File: KAPP/822-joss/110_3-6 457171(21p) - Page : 1/21 - Op: SD - Time: 07:12 - Date: 18:11:2002 ...
08 Prokaryotes
... Scattered within bacterial cytoplasm are numerous small loops of DNA known as plasmids. ...
... Scattered within bacterial cytoplasm are numerous small loops of DNA known as plasmids. ...
Я-Amyloid-Induced Synthesis of the Ganglioside Gd3 Is a Requisite
... AP-treated cortical neurons express the repertoire of proteins necessary to exit quiescence and eventually enter S phase. These neurons undergo apoptosis before entering the G2/M phase (Copani et al., 1999). Because AP-induced activation of this “neuronal cycle” seems to be critical for the develo ...
... AP-treated cortical neurons express the repertoire of proteins necessary to exit quiescence and eventually enter S phase. These neurons undergo apoptosis before entering the G2/M phase (Copani et al., 1999). Because AP-induced activation of this “neuronal cycle” seems to be critical for the develo ...
Actin Cytoskeleton in Plants: From Transport Networks to Signaling
... for indirect immunofluorescence microscopy after highpressure freeze-substitution (Ding et al., 1992; Roy et al., 1997), formaldehyde fixation (Baluška et al., 1997a; Blancaflor and Hasenstein, 1997; Vitha et al., 1997; Wasteneys et al., 1997), immunogold transmission EM (Lancelle et al., 1987; Lan ...
... for indirect immunofluorescence microscopy after highpressure freeze-substitution (Ding et al., 1992; Roy et al., 1997), formaldehyde fixation (Baluška et al., 1997a; Blancaflor and Hasenstein, 1997; Vitha et al., 1997; Wasteneys et al., 1997), immunogold transmission EM (Lancelle et al., 1987; Lan ...
Contributions by members of the TGFbeta superfamily to lens
... 1981), withdraw from the cell cycle (Zwaan and Kenyon 1984) and differentiate into fiber cells (Fig. 2). Fiber cells elongate greatly (Bassnett and Winzenburger 2003) and synthesize and accumulate large amounts of a limited number of cytoplasmic proteins, the crystallins. Crystallin accumulation in ...
... 1981), withdraw from the cell cycle (Zwaan and Kenyon 1984) and differentiate into fiber cells (Fig. 2). Fiber cells elongate greatly (Bassnett and Winzenburger 2003) and synthesize and accumulate large amounts of a limited number of cytoplasmic proteins, the crystallins. Crystallin accumulation in ...
Adherence and uptake of Francisella into host cells
... monocytes.21,22 However, deletion of the mannose receptor does not completely eliminate Francisella internalization,21,22,50 implicating other host molecules in the internalization of unopsonized Francisella. Uptake of opsonized Francisella can be mediated by different cell surface receptors dependi ...
... monocytes.21,22 However, deletion of the mannose receptor does not completely eliminate Francisella internalization,21,22,50 implicating other host molecules in the internalization of unopsonized Francisella. Uptake of opsonized Francisella can be mediated by different cell surface receptors dependi ...
Enhancers of HIV Infection Phosphatase Form Amyloidogenic
... To further examine the mechanism underlying infectivity enhancement, we stained PAP85-120 fibrils with an amyloid-specific red fluorescent dye (ProteoStat amyloid plaque detection kit) (Fig. 4A). Fibrils stained by the dye were not impaired in their ability to enhance HIV-1 infection (data not shown ...
... To further examine the mechanism underlying infectivity enhancement, we stained PAP85-120 fibrils with an amyloid-specific red fluorescent dye (ProteoStat amyloid plaque detection kit) (Fig. 4A). Fibrils stained by the dye were not impaired in their ability to enhance HIV-1 infection (data not shown ...
Chromatin organization and global regulation of Hox gene clusters
... nuclear space [1–3]. These large-scale approaches tentatively define global relationships between the chromatin structure observed at a particular genomic locus, the three-dimensional organization of this locus and the associated gene activity. For instance, both local chromatin post-translational m ...
... nuclear space [1–3]. These large-scale approaches tentatively define global relationships between the chromatin structure observed at a particular genomic locus, the three-dimensional organization of this locus and the associated gene activity. For instance, both local chromatin post-translational m ...
Saccharomyces cerevisiae Genes Involved in Survival of Heat Shock
... wall remodeling (Imazu and Sakurai 2005), ubiquitin-related protein degradation, DNA- and RNA-modifying enzymes, metabolic enzymes, regulatory proteins, and others involved with maintenance of cell ...
... wall remodeling (Imazu and Sakurai 2005), ubiquitin-related protein degradation, DNA- and RNA-modifying enzymes, metabolic enzymes, regulatory proteins, and others involved with maintenance of cell ...
Seasonal Changes of Plasma Membrane H
... polarization and the apoplastic acidification drive the uptake of nutrients into cells via secondary active transport and cause the opening of ion channels, for instance K⫹ channels (Michelet and Boutry, 1995; Hoth et al., 1997; Maathuis et al., 1997). With respect to trees, numerous studies have sh ...
... polarization and the apoplastic acidification drive the uptake of nutrients into cells via secondary active transport and cause the opening of ion channels, for instance K⫹ channels (Michelet and Boutry, 1995; Hoth et al., 1997; Maathuis et al., 1997). With respect to trees, numerous studies have sh ...
Aerobic Fermentation and the Depletion of the Amino Acid Pool in
... A second point of interest in connection with Fig. i is that amino acid pool depletion does not occur until some 30 minutes after enzymatic activity, as measured by oxygen consumption, has appeared. Thus, cell respiration of itself does not appear to cause pool depletion. The work of Sheffner and Li ...
... A second point of interest in connection with Fig. i is that amino acid pool depletion does not occur until some 30 minutes after enzymatic activity, as measured by oxygen consumption, has appeared. Thus, cell respiration of itself does not appear to cause pool depletion. The work of Sheffner and Li ...
Cyanidioschyzon merolae Genome. A Tool for
... Figure 1. The basic courses of mitosis in a typical eukaryotic cell and unicellular red alga (C. merolae) cell. As typical cells contain many double membrane- and single membrane-bound organelles, they are not illustrated. During interphase, the centrosome (CEN) forms outside the nucleus (N), inclu ...
... Figure 1. The basic courses of mitosis in a typical eukaryotic cell and unicellular red alga (C. merolae) cell. As typical cells contain many double membrane- and single membrane-bound organelles, they are not illustrated. During interphase, the centrosome (CEN) forms outside the nucleus (N), inclu ...
Localization of the Wilms` tumour protein WT1 in avian embryos
... were then rehydrated in TRIS-phosphate-buffered saline (TPBS) and the endogenous peroxidase activity was quenched by incubation for 30 min with 3% hydrogen peroxide in TPBS. After washing, non-specific binding sites were saturated for 30 min with 16% sheep serum, 1% bovine serum albumin and 0.5% Tri ...
... were then rehydrated in TRIS-phosphate-buffered saline (TPBS) and the endogenous peroxidase activity was quenched by incubation for 30 min with 3% hydrogen peroxide in TPBS. After washing, non-specific binding sites were saturated for 30 min with 16% sheep serum, 1% bovine serum albumin and 0.5% Tri ...
Origins of neural crest cells and cranial placodes at the edge of the
... Semaphorin is expressed on the posterior somite ...
... Semaphorin is expressed on the posterior somite ...
Specific vasopressin binding to rat adrenal glomerulosa cells
... Cells from the zona glomerulosa of rat adrenals were isolated and maintained for 3 days in primary culture. Specific vasopressin binding was determined by using [3H]vasopressin. [3H]Vasopressin binding was time-dependent (half-time of about 2 min for 6 nm free ligand) and reversible on addition of u ...
... Cells from the zona glomerulosa of rat adrenals were isolated and maintained for 3 days in primary culture. Specific vasopressin binding was determined by using [3H]vasopressin. [3H]Vasopressin binding was time-dependent (half-time of about 2 min for 6 nm free ligand) and reversible on addition of u ...
Phenolic compounds isolated from Pilea microphylla prevent
... After exposure, the cell cultures were transferred to a 5% CO2 incubator and allowed to grow up for the stipulated time period pertaining to the assay at 37 1C. 2.6.3. Measurement of reactive oxygen species (ROS) Intracellular levels of ROS were estimated using a cell permeable oxidation sensitive p ...
... After exposure, the cell cultures were transferred to a 5% CO2 incubator and allowed to grow up for the stipulated time period pertaining to the assay at 37 1C. 2.6.3. Measurement of reactive oxygen species (ROS) Intracellular levels of ROS were estimated using a cell permeable oxidation sensitive p ...
Specification of the C. elegans MS blastomere by the T
... Fig. 2. The tbx-35 gene encodes a T-box factor. (A) Gene structure of tbx-35 on LGII. The left end of the gene is preceded by the start codon of ZK177.1. The deleted regions in tm1789 and tm618 are shown above the gene. The predicted mRNA is shown below. The coding region is shaded, with the T-box e ...
... Fig. 2. The tbx-35 gene encodes a T-box factor. (A) Gene structure of tbx-35 on LGII. The left end of the gene is preceded by the start codon of ZK177.1. The deleted regions in tm1789 and tm618 are shown above the gene. The predicted mRNA is shown below. The coding region is shaded, with the T-box e ...
Perspectives in the Coordinate Regulation of Cell Cycle Events in
... C period as well. The problem in deciphering the genetic mechanisms that regulate genes that occur at specific phases and perform totally different cell events has been described earlier. This dilemma arises again at this critical phase of the cell cycle. Genes involved in chromosome condensation/se ...
... C period as well. The problem in deciphering the genetic mechanisms that regulate genes that occur at specific phases and perform totally different cell events has been described earlier. This dilemma arises again at this critical phase of the cell cycle. Genes involved in chromosome condensation/se ...
Phosphorylation-Dependent Regulation of Septin
... Cdc12, and Shs1/Sep7, were investigated. Each septin was tagged individually at its N terminus with GFP and expressed under the control of its own promoter. Half of the fluorescently labeled septin ring was irradiated with a laser beam to irreversibly bleach GFP. Fluorescence was then monitored over ...
... Cdc12, and Shs1/Sep7, were investigated. Each septin was tagged individually at its N terminus with GFP and expressed under the control of its own promoter. Half of the fluorescently labeled septin ring was irradiated with a laser beam to irreversibly bleach GFP. Fluorescence was then monitored over ...
Title The molecular architecture of the plant nuclear
... that the bulk of the NPC structures evolved through a series of gene duplications and ...
... that the bulk of the NPC structures evolved through a series of gene duplications and ...
Corresponding author: Dr. A. Lacey Samuels
... breakdown products were consistently shown to be radioactive, indicating incorporation of 3H-phenylalanine into the lignin polymer (Supplemental Figure 1). To investigate the nature of the soluble metabolites that were labeled during the four hour incubation with 3H-phenylalanine, methanol soluble p ...
... breakdown products were consistently shown to be radioactive, indicating incorporation of 3H-phenylalanine into the lignin polymer (Supplemental Figure 1). To investigate the nature of the soluble metabolites that were labeled during the four hour incubation with 3H-phenylalanine, methanol soluble p ...