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Lecture 23 - Biology of Cancer
Lecture 23 - Biology of Cancer

... How is PDT used to treat cancer? In the first step of PDT for cancer treatment, a photosensitizing agent is injected into the bloodstream. The agent is absorbed by cells all over the body, but stays in cancer cells longer than it does in normal cells. Approximately 24 to 72 hours after injection whe ...
Programmed cell death in C. elegans: the genetic framework
Programmed cell death in C. elegans: the genetic framework

Light Modulates the Biosynthesis and
Light Modulates the Biosynthesis and

... carboxysome numbers. The light dependence of carboxysome content was further substantiated by transmission electron microscopy results of wild-type Synechococcus cells (Fig. 2B; Supplemental Fig. S3). The numbers and positioning of carboxysomes in the cell were statistically analyzed based on the co ...
Growth Factors
Growth Factors

...  History: In 1957, researchers observed that susceptible animal cells, if they were exposed to a colonizing virus, immediately became resistant to attack by other viruses. This resistance was induced by a substance secreted by virally infected cells which was named interferon  It has been shown th ...
Limbal stem cell deficiency and its management
Limbal stem cell deficiency and its management

... may be necessary to reduce dry eye. Only once eyelid disease and dry eye have been treated effectively should one consider surgical management of limbal stem cell deficiency. The surgical options for limbal stem cell deficiency are based on the transplantation of healthy limbal epithelium. This can ...
Precipitation of calcium, magnesium, strontium and barium in tissues
Precipitation of calcium, magnesium, strontium and barium in tissues

... Prismatic crystals were observed in cells associated with fibre cells in phyllodes of all four species collected from mature plants grown in the field, and all prismatic crystals were birefringent (Fig. 1). There were also other forms of crystals in phyllodes of A. robeorum, thought to be crystal sa ...
Comparative assessment of heavy metal removal by
Comparative assessment of heavy metal removal by

... Cu, Cd and Pb at pH 7, 6 and 6 respectively which are similar to the results of Wang and Chen (2006) and Blackwell et al. (1995). In their result, the highest adsorption occurs at pH ranges from 4 - 8. This pH range is widely accepted as being optimal for metal uptake of almost all types of biomass. ...
A family of abundant plasma membrane
A family of abundant plasma membrane

... Bound antibody was visualized with a 2,000× dilution of the rabbit horseradish peroxidase-conjugated anti-rat lg antiserum described for immunoblotting, but for ELISA tetramethylbenzidine was used in the developing solution. In certain instances the immobilized membranes were treated before addition ...
Neuron-to-Cell Spread of Pseudorabies Virus in a
Neuron-to-Cell Spread of Pseudorabies Virus in a

... Trichamber culture system. All Teflon rings were purchased from Tyler Research (Alberta, Canada) and protocols were modified from previously published reports for Campenot chambers (8). Briefly, all the tools and reagents including the Teflon rings and silicone grease-loaded syringe (Dow Corning) we ...
PDF
PDF

... screening of this and other Chlorophycean taxa will be required to ascertain the similarity of these polymers with those of the CGA and embryophytes. However, in the Chlamydomonas–Volvox assemblage (i.e., volvocalean flagellates), the cell wall does not contain cellulose but is made of crystalline gl ...
Microtubule Associated Protein 1b (MAP1B) Is a Marker of the
Microtubule Associated Protein 1b (MAP1B) Is a Marker of the

... expected further similarities in the expression of microtubule associated proteins (Maps). Neurons express a specific set of Maps that are generally considered to be scaffold proteins [6, 7]. There are two classical Map families: The MAP1 family (MAP1A, MAP1B and MAP1S) and the MAP2/TAU family (MAP2 ...
Full Text Article
Full Text Article

... and identification of therapeutically important products. Actinomycetes are gram-positive bacteria with high guanine + cytosine content of over 55% in their DNA, which have been recognized as sources of several secondary metabolites, antibiotics, and bioactive compounds that affect microbial growth ...
Campbell Biology, 10e (Reece) Chapter 7 Membrane Structure and
Campbell Biology, 10e (Reece) Chapter 7 Membrane Structure and

... Answer: B Bloom's Taxonomy: Application/Analysis Section: 7.3 37) What will happen to a red blood cell (RBC), which has an internal ion concentration of about 0.9 percent, if it is placed into a beaker of pure water? A) The cell would shrink because the water in the beaker is hypotonic relative to t ...
Bactericidal Antibiotics Induce Toxic Metabolic - Collins Lab
Bactericidal Antibiotics Induce Toxic Metabolic - Collins Lab

... was unable to distinguish between the Kan and Amp biological replicates at this time point, suggesting that these antibiotics, in particular, elicited a similar metabolic response. The Nor replicates formed a separate cluster due to a set of uniquely decreased metabolites (largely populated by amino ...
ovarian malignancy
ovarian malignancy

... mucinous tumour of the ovary, a well differentiated appendiceal carinoma, any other gastrointestinal primary tumour or a mucocele of the appendix. Care should be taken to avoid rupture during surgery ...
Membrane receptors in the gastrointestinal tract
Membrane receptors in the gastrointestinal tract

... M1 and M2. However, it has been demonstrated recently that a single type of muscarinic receptor, subtype M2, might be coupled with adenylate cyclase and phosphoinositides in Chinese hamster ovary cells after transfection with a vector directing the expression of the porcine atrial M2-type receptor ( ...
From yeast to patient neurons and back again - Lindquist Lab
From yeast to patient neurons and back again - Lindquist Lab

... a-syn? And, if so, might we take advantage of yeast genetics to identify that compound’s protein target and mechanism of action? The concept was to use the cells themselves to identify a “druggable” node to rescue cells from a-syn toxicity, without introducing our own prejudices about what might be ...
Applied and Environmental Microbiology
Applied and Environmental Microbiology

... gfp-marked strain, samples were surface sterilized with 70% ethanol for 5 min (roots) or 3 min (fifth internode and fifth leaf), followed by 1% commercial bleach and a 0.01% Tween 20 solution for 1 min, and then washed three times in distilled water (1 min each time). The samples were then ground an ...
mycoplasmas in tissue culture
mycoplasmas in tissue culture

... for the detection of mycoplasma tissue-culture contaminants than seeding test material into a line of mouse lymphoma cells in vitro. This line showed cytopathic changes when infected with some mycoplasmas. The lymphoma cells also supported the growth of some non-cytopathogenic strains of mycoplasma ...
PDF
PDF

... Ectoderm-mesenchyme interactions are known to play an important role during chick limb morphogenesis (Gumpel-Pinot, 1972,1973,1980,19Sla,b,c). An important part of these interactions is taken over by the apical ectodermal ridge and underlying mesenchyme (Saunders & Gasseling, 1968; Zwilling, 1956a,b ...
A Dominant Negative Mutant of Cyclin-Dependent
A Dominant Negative Mutant of Cyclin-Dependent

... Cells in maize (Zea mays) endosperm undergo multiple cycles of endoreduplication, with some attaining DNA contents as high as 96C and 192C. Genome amplification begins around 10 d after pollination, coincident with cell enlargement and the onset of starch and storage protein accumulation. Although t ...
Chapter 3 Cells- Structure & Function Part II
Chapter 3 Cells- Structure & Function Part II

...  Cells have two major periods  Interphase  Cell grows  Cell carries on metabolic processes ...
Potassium regulation
Potassium regulation

... cell causing hyperkalemia • For each 10 mOsm increase in osmalrity, this will make 0.4-0.8 mEqv increase in potassium extracellular concentration • Remember that the normal osmolarity in the plasma is 284mOsm • -Epinephrine pushes potassium inside the cells, so giving beta blocker as propranolol wil ...
1 Laccases direct lignification in the discrete secondary cell wall
1 Laccases direct lignification in the discrete secondary cell wall

... including irregular xylem and a marked reduction in lignin deposition (Pro35S:miRNA C4H lines, Supplemental Fig. 1; Pro35S:miRNA CCR lines, Smith et al., 2013). Constitutive expression of the C4H or CCR1 microRNA during VND7induced TE formation did not inhibit secondary cell wall formation (Fig. 1D, ...
My Life with Dicty
My Life with Dicty

... collect growing Dictyostelium amoebae and initiate synchronous development. For the next 24 hours my attention was totally focused on the developing cells as they aggregated, formed slugs, and culminated into fruiting bodies. I was enamored but did not realize for several years that it would be a li ...
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Amitosis

Amitosis (a- + mitosis) is absence of mitosis, the usual form of cell division in the cells of eukaryotes. There are several senses in which eukaryotic cells can be amitotic. One refers to capability for non-mitotic division and the other refers to lack of capability for division. In one sense of the word, which is now mostly obsolete, amitosis is cell division in eukaryotic cells that happens without the usual features of mitosis as seen on microscopy, namely, without nuclear envelope breakdown and without formation of mitotic spindle and condensed chromosomes as far as microscopy can detect. However, most examples of cell division formerly thought to belong to this supposedly ""non-mitotic"" class, such as the division of unicellular eukaryotes, are today recognized as belonging to a class of mitosis called closed mitosis. A spectrum of mitotic activity can be categorized as open, semi-closed, and closed mitosis, depending on the fate of the nuclear envelope. An exception is the division of ciliate macronucleus, which is not mitotic, and the reference to this process as amitosis may be the only legitimate use of the ""non-mitotic division"" sense of the term today. In animals and plants which normally have open mitosis, the microscopic picture described in the 19th century as amitosis most likely corresponded to apoptosis, a process of programmed cell death associated with fragmentation of the nucleus and cytoplasm. Relatedly, even in the late 19th century cytologists mentioned that in larger life forms, amitosis is a ""forerunner of degeneration"".Another sense of amitotic refers to cells of certain tissues that are usually no longer capable of mitosis once the organism has matured into adulthood. In humans this is true of various muscle and nerve tissue types; if the existing ones are damaged, they cannot be replaced with new ones of equal capability. For example, cardiac muscle destroyed by heart attack and nerves destroyed by piercing trauma usually cannot regenerate. In contrast, skin cells are capable of mitosis throughout adulthood; old skin cells that die and slough off are replaced with new ones. Human liver tissue also has a sort of dormant regenerative ability; it is usually not needed or expressed but can be elicited if needed.
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