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Mitochondria as signaling organelles R E V I E W Open Access
Mitochondria as signaling organelles R E V I E W Open Access

... transport chain (ETC), which pumps protons across the mitochondrial inner membrane to generate an electrochemical gradient that is required both for production of ATP and for the efficient shuttling of proteins into and out of mitochondria. Mitochondrial ATP generation allows cells to maintain a hig ...
how exercise builds muscle
how exercise builds muscle

... myofibrils. These components, called sarcomeres, are the contractile units of muscle; a myofibril contracts when all its sarcomeres do so. Sarcomeres are about 2.2 microns long and are linked end to end to make up a myofibril. Like Russian nesting dolls, muscle’s components keep getting smaller: wit ...
Craniofacial Development and the Evolution of the Vertebrates: the
Craniofacial Development and the Evolution of the Vertebrates: the

... modern treatment of the issue). However, this was never truly integrated with the transcendental morphology until recently, when molecular developmental biology became the glue to unite them (see de Beer, 1926, 1937, 1958; Jarvik, 1980; Couly et al., 1993, 1998; see Hanken and Hall, 1993, for studie ...
Cellular lipidomics
Cellular lipidomics

... generation does not make science. While powerful data analysis is a first requirement, the outcome is useful only to the extent that it answers a scientific question. Cell biologists must find out how metabolomics can be applied to further our understanding of the living cell in health and disease. ...
Laboratory Research, Cloning, and Human Cell Lines
Laboratory Research, Cloning, and Human Cell Lines

... and these are currently being drafted. The effect of the changes to these regulations will be to more clearly and accurately define the scope of low-risk GMO developments in laboratory containment facilities. While these changes will correct some current anomalies in the regulations and allow some a ...
THE ROLE OF FIBROBLAST GROWTH FACTOR (FGF) AND TYPE
THE ROLE OF FIBROBLAST GROWTH FACTOR (FGF) AND TYPE

... the sutural tissue is not restricted to being an articulation but appears to include: 1) to unite bones, while allowing minor movements; 2) to act as growth áreas; and 3) to absorb mechanical stress, thus protecting its osteogenic tissue60 and the neural structures beneath. Pritchard et al61 stated ...
+Tec - Blood Journal
+Tec - Blood Journal

... Zap-70, Syk, Tyk-2, Jak-l, and Jak-2).’ Well-shared characters among the members of the Src-family are (1) an Nterminal myristylation site: (2) a C-terminal tyrosine residue as the negative regulatory site (corresponding to Tyr-527 in c - S r ~ ) and , ~ (3) Src homology (SH) 2 and SH3 domains in th ...
Antisickling Activity and Membrane Stabilizing Effect of
Antisickling Activity and Membrane Stabilizing Effect of

... Each year approximately 100,000 children in the world are born with sickle cell disease (SCD) which is a genetic disorder. This disease is considered as a public health problem in many countries, but with a major burden in Africa particularly in tropical regions in west and central Africa [1-5]. SCD ...
Functions of Skeletal Muscle Tissue
Functions of Skeletal Muscle Tissue

... lie parallel to one another in the direction of the long axis of the cell. They are not arranged in a definite striped (striated) pattern, as in skeletal muscles - hence the name smooth muscle . Smooth muscle fibres interlace to form sheets or layers of muscle tissue rather than bundles. Smooth musc ...
- D-Scholarship@Pitt
- D-Scholarship@Pitt

... Abstract. The trial and error of the pioneering xenotransplant trials over the past three decades has defined the limitation of the species used. Success was tantalizingly close with the chimpanzee, baboon, and other primates. The use of more disparate species has been frustrated by the xenoantibody ...
Journal of Phycology
Journal of Phycology

... lipids in the chloroplast fraction (Fig. 7b, lane 3). Isochrysis galbana and E. huxleyi cultures produced similar results, but E. huxleyi lipids associated with LB were more difficult to recover after centrifugation, even when cells had notable lipid vesicles (not shown). The GC analysis of cell fra ...
C. elegans - York College of Pennsylvania
C. elegans - York College of Pennsylvania

... Figure 2: C. elegans on a control plate containing HT115 bacterial cells with plasmid 1. Plate was imaged 48 hours after initial transfer of C. elegans. The area circled indicates the disturbed areas of bacteria in which the C. elegans has already traveled over. The fact that there are increased amo ...
Fluorescent properties of c-type cytochromes
Fluorescent properties of c-type cytochromes

Slits affect the timely migration of neural crest cells via robo receptor
Slits affect the timely migration of neural crest cells via robo receptor

... Slit1 (A), Slit2 (B), Slit3 (C), Robo1 (D), and Robo2 (E) anti-sense probes. HH12–13 chicken embryos showed expression of Slit ligands in dorsal neural tube (arrows in H–K sections for Slit1 and Slit2). Robo1 receptor is expressed in the medial somites (red arrow in D) but also in the neural tube at ...
as a PDF
as a PDF

... from the same site (Fig. 1A, Movies S1,S2). Limited examples of severing or breakage in elongated microtubules were recorded, though in 24 of 51 observed severing events at least one of the resulting microtubules depolymerized to extinction. In observations of 30 cells, we failed to observe a microt ...
"Molecular Motors in Plant Cells". In: Molecular Motors
"Molecular Motors in Plant Cells". In: Molecular Motors

... Molecular motors regulate diverse cellular functions including the organization and dynamics of microtubule (MT) and actin cytoskeleton, cytoplasmic streaming, cell polarity, cell growth, morphogenesis, chromosome segregation and transport of vesicles, organelles and macromolecular complexes. In euk ...
Inhibition of copepod grazing by diatom exudates: a factor in the
Inhibition of copepod grazing by diatom exudates: a factor in the

... have been shown to be involved in the attachment of cells to surfaces and cell-cell adhesion, producing multicellular aggregates (Yoshida 1991). Interparticle bridging by polysaccharide polymers is thought to be important in formation of larger organic aggregates in the sea (Alldredge & McGillivary ...
An analysis of the response to gut induction in the C. elegans embryo
An analysis of the response to gut induction in the C. elegans embryo

... 3NB12 monoclonal antibody (Priess and Thomson, 1987). Body wall muscle differentiation was assayed using a polyclonal antibody to paramyosin kindly provided by Hiroaki Kagawa. Hypodermal differentiation was assayed using a polyclonal antibody to LIN-26 protein, which stains some descendants of isola ...
Exocytosis and cell polarity in plants exocyst and recycling domains
Exocytosis and cell polarity in plants exocyst and recycling domains

... release of the GTPase Arf1 from Golgi membranes in tissue culture cells (Langhans & Robinson, 2007). 4,4-Difluoro-5,7dimethyl-4-bora-3a,4a-diaza-S-indacene (BODIPY® FL)labelled PA localized, on prolonged exposure, into Golgi bodies in pollen tubes (Potocký et al., 2003). Arabidopsis mammalianlike PL ...
Dependence of an Adenosine-Activated Potassium Current on a
Dependence of an Adenosine-Activated Potassium Current on a

... pg/ml and stored for up to 2 d at 0-4°C. Purine nucleotides were obtained from Boehringer Mannheim and Sigma. ...
Arabidopsis ORGAN SIZE RELATED1 regulates organ growth and
Arabidopsis ORGAN SIZE RELATED1 regulates organ growth and

... In Arabidopsis leaves, most cells begin to undergo cell expansion when cell proliferation arrests gradually from leaf tip to base; however, there are still some cells that maintain the meristematic competence to continue dividing and form the specific cell type within each cell layer (Donnelly et al ...
Frequent exchange of the DNA polymerase during bacterial
Frequent exchange of the DNA polymerase during bacterial

... synthesis is performed by the Pol III polymerase (aeq). Three copies of Pol III are incorporated into the replisome through an interaction with the t subunit of the pentameric clamp loader complex (t3dd’). Together, these constitute the Pol III* subassembly ((aeq)3-t3dd’). The clamp loader is also r ...
Accumulation of autophagic vacuoles and cardiomyopathy in LAMP
Accumulation of autophagic vacuoles and cardiomyopathy in LAMP

... in the index case of Danon’s disease17 and nine other unrelated patients with this familial disease, which is characterized by fatal cardiomyopathy, variable mental retardation and mild myopathy18. Ultrastructurally, the accumulation of vacuoles within skeletal and cardiac muscle is a prominent find ...
Lightning-triggered electroporation and electrofusion as possible
Lightning-triggered electroporation and electrofusion as possible

... [19,20], were rare and largely ignored. With extensive DNA sequencing, however, it emerged that a number of genes in eukaryotes are absent from archaeal genomes, yet present in phylogenetically more distant bacteria [21,22], and it also became increasingly clear that the phylogenetic trees charted f ...
PDF
PDF

... material) (Saint-Amant and Drapeau, 1998). By contrast, mi310 mutants did not respond to touch and remained motionless in the water (Fig. 1E-H; see Movie 2 in the supplementary material). To test whether the motor system is dysfunctional in mutants, we observed spontaneous swimming following bath ap ...
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Amitosis

Amitosis (a- + mitosis) is absence of mitosis, the usual form of cell division in the cells of eukaryotes. There are several senses in which eukaryotic cells can be amitotic. One refers to capability for non-mitotic division and the other refers to lack of capability for division. In one sense of the word, which is now mostly obsolete, amitosis is cell division in eukaryotic cells that happens without the usual features of mitosis as seen on microscopy, namely, without nuclear envelope breakdown and without formation of mitotic spindle and condensed chromosomes as far as microscopy can detect. However, most examples of cell division formerly thought to belong to this supposedly ""non-mitotic"" class, such as the division of unicellular eukaryotes, are today recognized as belonging to a class of mitosis called closed mitosis. A spectrum of mitotic activity can be categorized as open, semi-closed, and closed mitosis, depending on the fate of the nuclear envelope. An exception is the division of ciliate macronucleus, which is not mitotic, and the reference to this process as amitosis may be the only legitimate use of the ""non-mitotic division"" sense of the term today. In animals and plants which normally have open mitosis, the microscopic picture described in the 19th century as amitosis most likely corresponded to apoptosis, a process of programmed cell death associated with fragmentation of the nucleus and cytoplasm. Relatedly, even in the late 19th century cytologists mentioned that in larger life forms, amitosis is a ""forerunner of degeneration"".Another sense of amitotic refers to cells of certain tissues that are usually no longer capable of mitosis once the organism has matured into adulthood. In humans this is true of various muscle and nerve tissue types; if the existing ones are damaged, they cannot be replaced with new ones of equal capability. For example, cardiac muscle destroyed by heart attack and nerves destroyed by piercing trauma usually cannot regenerate. In contrast, skin cells are capable of mitosis throughout adulthood; old skin cells that die and slough off are replaced with new ones. Human liver tissue also has a sort of dormant regenerative ability; it is usually not needed or expressed but can be elicited if needed.
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