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Antibody Selection for Immobilizing Living Bacteria
Antibody Selection for Immobilizing Living Bacteria

... against bacterial surface antigens associated with Salmonella enterica Serovar Typhimurium. The targeted bacterial antigens were CFA/I fimbriae, flagella, lipopolysaccharides (LPS), and capsular F1 antigen. The best immobilization of S. Typhimurium was achieved with the antibody against CFA/I fimbri ...
Cadherins constitute a superfamily of transmembrane - HAL
Cadherins constitute a superfamily of transmembrane - HAL

... adherens junctions, this model is particularly of interest since it is supported by some of disparate results for adherens junctions. In particular, it is in agreement with the X-ray and electron microscopy results which demonstrate that the EC1 dimer is central to homotypic cadherin interaction. It ...
Full-Text PDF
Full-Text PDF

... to the thymus and as they mature T cells express a clonally restricted T cell receptor (TCR) and develop into two major lineages referred to as TCRαβ+ and TCRγδ+ . The TCRαβ+ lineage represents the major population of T cells in the peripheral immune system comprising >90% of the total T cells. Thes ...
TOPIC 1. CELL CHARACTERISTICS AND DIGESTIBILITIES What
TOPIC 1. CELL CHARACTERISTICS AND DIGESTIBILITIES What

... the evidence for chemical inhibitors of digestion in plants has been available for over 30 years. Knowledge of the effects of different inhibitors on diet digestibilities are not yet well understood, however. Fraenkal (1959) called attention to the role of secondary plant compounds as defense mechan ...
to the complete text
to the complete text

... were observed in a number of ascomycetes and have a duration of about 1 s. In addition, Lew (2005) demonstrated pressure gradients along hyphae of Neurospora crassa and suggested that they drive mass flow of cytoplasm. These pressure gradients are probably very small, no more than 10 kPa per mm (app ...
Conjugated linoleic acid decreases production
Conjugated linoleic acid decreases production

... Conjugated linoleic acid (CLA) is a dietary fatty acid that has received considerable attention due to its unique properties in rodent models including anti-cancer, anti-atherogenic and anti-diabetic effects. The effects of CLA are similar to those seen with ligands for peroxisome proliferator-activ ...
Molecular cloning, over-expression, developmental regulation and
Molecular cloning, over-expression, developmental regulation and

... The cDNA insert was digested from the vector with EcoRI and NotI, purified and used as probe. Radiolabelled DNA probes were synthesized by the method of Feinberg and Vogelstein (1983). For these probes, prehybridization and hybridization were carried out at 65°C using the method of Church and Gilber ...
Bachelor of Arts | Department of Biology
Bachelor of Arts | Department of Biology

... The biology Bachelor of Arts degree program provides a general background in biology, and has the most flexible scheduling of the three biology degrees offered. It is especially recommended for students who are pre-professional, have multiple majors, intend to do a junior year abroad or an internshi ...
Understanding the role of growth factors in embryonic development
Understanding the role of growth factors in embryonic development

... for transforming growth factor-b and Wnt/Frizzled families in regulating aspects of fibre differentiation. In the case of the epithelium, key roles for Wnt/b-catenin and Notch signalling have been demonstrated in embryonic development, but it is not known if other factors are required for its format ...
The Development of Novel Approaches to the Identification of
The Development of Novel Approaches to the Identification of

Systems-Wide Analysis of Acclimation Responses to Long
Systems-Wide Analysis of Acclimation Responses to Long

... Immediately after transfer to 25°C, TAG levels dropped slightly, but stayed constant during the remaining 8 h monitored in the recovery phase. In contrast to the other cellular macromolecules investigated, the starch content per cell declined within the first 2 h of HS by 28%, but during the remainin ...
(Hsp90) inhibitors
(Hsp90) inhibitors

... molecules. Although pull-down assays using tagged molecules of classical Hsp90 inhibitors have been published, close analysis of these data suggest that the molecules do not target Hsp90 alone but rather have non-specific binding affinities for multiple proteins[5c, 6]. In the past 10 years over 10, ...
Ionic and Osmotic Effects of NaCl-Induced
Ionic and Osmotic Effects of NaCl-Induced

... of Synechocystis sp. (Kaneko et al., 1996) includes at least three putative genes for K⫹ channels. The K⫹ channels in prokaryotes (Murata et al., 1996; Nakamura et al., 1998) and in higher plants (Schachtman et al., 1991; Murata et al., 1994; Tyerman et al., 1997) are permeable to Na⫹ ions. Thus, su ...
PDF
PDF

... Epithelial cells are linked by apicolateral junctions that are essential for tissue integrity. Epithelial cells also secrete a specialized apical extracellular matrix (ECM) that serves as a protective barrier. Some components of the apical ECM, such as mucins, can influence epithelial junction remod ...
4-3. Cell wall structure of E. coli and B. subtilis
4-3. Cell wall structure of E. coli and B. subtilis

... family and are located in the outer membrane (Table 1) [66, 67]. The function of MltD is probably wall recycling [69]. Recently, MltF was reported as an outer membrane-bound periplasmic protein and its C-terminal is a catalytic domain and the N-terminal appears to modulate the lytic behavior of the ...
COMMUNICATION Redefining the phenotype of Heat shock protein 90
COMMUNICATION Redefining the phenotype of Heat shock protein 90

... molecules. Although pull-down assays using tagged molecules of classical Hsp90 inhibitors have been published, close analysis of these data suggest that the molecules do not target Hsp90 alone but rather have non-specific binding affinities for multiple proteins[5c, 6]. In the past 10 years over 10, ...
[PDF]
[PDF]

... hsa-mir-196a-2 ...
filtration of a bacterial fermentation broth: harvest conditions
filtration of a bacterial fermentation broth: harvest conditions

... equation (3) assumes this plot being linear. Some authors have reported that it was not always true implying differing period for cake build-up mechanism: an early period caused by the contact between broth components and membrane material and a subsequent period ...
Dynamin and the Actin Cytoskeleton Cooperatively Regulate
Dynamin and the Actin Cytoskeleton Cooperatively Regulate

... systematic comparison between BAR domain- and FCH domain-containing proteins. Sequence comparisons and structural predictions suggested that the FCH domain, together with the CC region that typically follows this domain, define a protein module similar to the BAR domain, which we have named the F-BA ...
Analysis of the Juxtamembrane Dileucine Motif in the Insulin Receptor
Analysis of the Juxtamembrane Dileucine Motif in the Insulin Receptor

... To investigate the importance of the dileucine pair at positions 986 and 987 in the juxtamembrane domain of the insulin receptor, we mutated the LeuLeu residues to MetSer and AlaAla, and expressed the various recombinant receptors in NIH-3T3 cells. Metabolic labeling studies confirmed that the mutan ...
PLANT CELL WALL PROTEINS
PLANT CELL WALL PROTEINS

... Annu. Rev. Plant. Physiol. Plant. Mol. Biol. 1998.49:281-309. Downloaded from arjournals.annualreviews.org by Shanghai Information Center for Life Sciences on 02/12/09. For personal use only. ...
SIMULATION OF PROKARYOTIC GENETIC CIRCUITS
SIMULATION OF PROKARYOTIC GENETIC CIRCUITS

LvNotch positions the ectoderm-endoderm boundary
LvNotch positions the ectoderm-endoderm boundary

... mRNA/fluorescein dextran injection into eight-cell-stage embryos Preparation of eight-cell stage embryos for injection into single blastomeres was identical to that described above, except that eggs were fertilized in 5 mM p-aminobenzoic acid (PABA), which was washed out immediately after fertilizat ...
Mitochondria as signaling organelles R E V I E W Open Access
Mitochondria as signaling organelles R E V I E W Open Access

... transport chain (ETC), which pumps protons across the mitochondrial inner membrane to generate an electrochemical gradient that is required both for production of ATP and for the efficient shuttling of proteins into and out of mitochondria. Mitochondrial ATP generation allows cells to maintain a hig ...
how exercise builds muscle
how exercise builds muscle

... myofibrils. These components, called sarcomeres, are the contractile units of muscle; a myofibril contracts when all its sarcomeres do so. Sarcomeres are about 2.2 microns long and are linked end to end to make up a myofibril. Like Russian nesting dolls, muscle’s components keep getting smaller: wit ...
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Amitosis

Amitosis (a- + mitosis) is absence of mitosis, the usual form of cell division in the cells of eukaryotes. There are several senses in which eukaryotic cells can be amitotic. One refers to capability for non-mitotic division and the other refers to lack of capability for division. In one sense of the word, which is now mostly obsolete, amitosis is cell division in eukaryotic cells that happens without the usual features of mitosis as seen on microscopy, namely, without nuclear envelope breakdown and without formation of mitotic spindle and condensed chromosomes as far as microscopy can detect. However, most examples of cell division formerly thought to belong to this supposedly ""non-mitotic"" class, such as the division of unicellular eukaryotes, are today recognized as belonging to a class of mitosis called closed mitosis. A spectrum of mitotic activity can be categorized as open, semi-closed, and closed mitosis, depending on the fate of the nuclear envelope. An exception is the division of ciliate macronucleus, which is not mitotic, and the reference to this process as amitosis may be the only legitimate use of the ""non-mitotic division"" sense of the term today. In animals and plants which normally have open mitosis, the microscopic picture described in the 19th century as amitosis most likely corresponded to apoptosis, a process of programmed cell death associated with fragmentation of the nucleus and cytoplasm. Relatedly, even in the late 19th century cytologists mentioned that in larger life forms, amitosis is a ""forerunner of degeneration"".Another sense of amitotic refers to cells of certain tissues that are usually no longer capable of mitosis once the organism has matured into adulthood. In humans this is true of various muscle and nerve tissue types; if the existing ones are damaged, they cannot be replaced with new ones of equal capability. For example, cardiac muscle destroyed by heart attack and nerves destroyed by piercing trauma usually cannot regenerate. In contrast, skin cells are capable of mitosis throughout adulthood; old skin cells that die and slough off are replaced with new ones. Human liver tissue also has a sort of dormant regenerative ability; it is usually not needed or expressed but can be elicited if needed.
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