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Molecular Cloning of CD68, a Human Macrophage
Molecular Cloning of CD68, a Human Macrophage

... the body and display great structural and functional heterogeneity, reflectingconditions within their local environment. Tissue macrophages are involved in many immune functions, the most significantbeing phagocytosis of foreign and necrotic material, and antigen processing and presentation.2 The su ...
Farnesoid X Receptor Ligands Inhibit Vascular Smooth Muscle Cell
Farnesoid X Receptor Ligands Inhibit Vascular Smooth Muscle Cell

... FXR knockout mice have severe dyslipidaemia37 and when crossed with apolipoprotein E (apoE) knockouts produce male offspring with enhanced atherosclerotic lesion formation as well as increased mortality when fed with a high-fat Western style diet, as might be expected.38 In contrast, male FXR⫺/⫺/ lo ...
PDF
PDF

... material) (Saint-Amant and Drapeau, 1998). By contrast, mi310 mutants did not respond to touch and remained motionless in the water (Fig. 1E-H; see Movie 2 in the supplementary material). To test whether the motor system is dysfunctional in mutants, we observed spontaneous swimming following bath ap ...
Residue-specific Mass Signatures for the
Residue-specific Mass Signatures for the

... temperature. Mononucleosomes purified from control and irradiated cells were incubated with the antibody against H2A.X or phosphorylated H2A.X, respectively, for 4 h at 4 °C. Proteinantibody conjugates were precipitated by protein A Sepharose at 4 °C for 2 h. The precipitate was washed three times w ...
p53 transcriptional activity is essential for p53dependent apoptosis
p53 transcriptional activity is essential for p53dependent apoptosis

... further highlighted by the creation of p53±/± mice, which are highly cancer prone and develop a large spectrum of tumors (Donehower et al., 1992; Jacks et al., 1994). It has become clear that p53 has at least two roles in preventing cancer: cell cycle arrest in G1, which allows time for the repair o ...
Regulative germ cell specification in axolotl embryos: a primitive trait
Regulative germ cell specification in axolotl embryos: a primitive trait

... urodele embryos PGCs do not develop from predetermined cells. Rather, urodele PGCs appear to develop from unspecialized mesodermal tissue that has responded to appropriate extracellular influences. Therefore the embryos of urodele and anuran amphibians have diverged in the way that they produce PGCs ...
THYMIC REQUIREMENT FOR CYCLICAL IDIOTYPIC AND
THYMIC REQUIREMENT FOR CYCLICAL IDIOTYPIC AND

... Thymic reconstitution of nude mice was accomplished by implanting a single neonatal BALB/c thymus beneath the capsule of each of the recipient's kidneys (thymus gland-reconstituted nude [TG-nude] mice) as described by Dukor et al (9). All TG-nude mice were examined for evidence of a successful thymi ...
An Early Tobacco Mosaic Virus-Induced Oxidative Burst in Tobacco
An Early Tobacco Mosaic Virus-Induced Oxidative Burst in Tobacco

... Whereas phase I and II bursts differ kinetically, they may also differ as to the source of ROS and/or the type of ROS produced. The exact kinetics of ROS induction is a function of the biology of the invading pathogen. Phase I responses may well be important in defense as a priming mechanism for lat ...
Fungi
Fungi

... – Are defined by the production of sexual spores in saclike asci, which are usually contained in fruiting bodies called ascocarps – Have asexual reproduction by producing enormous numbers of asexual spores called conidia ...
as PDF
as PDF

... L-Ascorbic acid (AA), commonly known as vitamin C, is considered one of the organism’s most powerful antioxidant agents due to its capacity to donate two electrons from its double link, that of positions two and three; thus, it interacts with the FR, blocking their harmful effect. The human body is ...
On the origins of cells: a hypothesis for the evolutionary transitions
On the origins of cells: a hypothesis for the evolutionary transitions

... 3.8 Gyr, because carbon isotope data provide evidence for biological CO2 fixation in sedimentary rocks of that age (Mojzsis et al. 1996; Rosing 1999; Nisbet & Sleep 2001; Ueno et al. 2002). By 3.5 Gyr, stromatolites were present, preserved microbial mats indicative of deposition by photosynthetic pr ...
Expression and immunogenicity of the entire human T cell
Expression and immunogenicity of the entire human T cell

... the two recombinant AcNPV clones (VHB5 and VHB6) at a multiplicity of 0.5. Three days post-infection (p.i.), the cells were harvested, washed and resuspended in PBS (pH 7.3) at a density of 5 x 106 cells/ml. Cell suspensions were spotted on tissue-grip (Fischer Scientific) coated glass slides, allow ...
The Forces Behind Cell Movement
The Forces Behind Cell Movement

... and are functionally polar in nature [1,12]. This means that they have two distinct ends: a fast and a slow growing end (called the plus end and minus end respectively) [1,12]. The minus end has a critical actin monomer concentration that is ~6 times higher than that at the plus end (~0.6 μM and ~0. ...
Homogeneous generation of iDA neurons with high similarity to
Homogeneous generation of iDA neurons with high similarity to

... generate iDA neurons that closely resemble midbrain DA neurons [6]. However, when we prepared ES cells harboring all 6 factors, they exhibited promiscuous differentiation even in the absence of dox, precluding their maintenance over multiple passages (data not shown). Thus, we removed the non-requis ...
bone matrix
bone matrix

... Osteoblasts are responsible for the synthesis of the organic components of bone matrix, consisting of type I collagen fibers, proteoglycans, and several glycoproteins including osteonectin. Osteoblasts are located exclusively at the surfaces of bone matrix, usually side by side in a layer somewhat r ...
A Simple and Efficient Method for Isolating Trichomes for
A Simple and Efficient Method for Isolating Trichomes for

Electron Microscopy of Intermediate Filaments: Teaming up with
Electron Microscopy of Intermediate Filaments: Teaming up with

... dense nodes of diameter 8 nm, giving the filaments a ‘faintly beaded appearance.’ Considering the later characterization and designation of these wavy filament arrays in mouse 3T3 fibroblasts as vimentin, referring to the latin vimentum for wickerwork or brushwood (Franke et al., 1978), it is quite ...
Heart healer - Cardiac and Vascular Biology
Heart healer - Cardiac and Vascular Biology

... unwanted side effects,” reveals Porrello. There are no guarantees that stem cell therapy will work at all, and a long way to go before any such therapy, if possible, becomes a reality, so in the meantime Porrello is ensuring he explores every avenue of investigation: “Stem cells might prove to be th ...
Stat3 and MMP7 Contribute to Pancreatic Ductal Adenocarcinoma
Stat3 and MMP7 Contribute to Pancreatic Ductal Adenocarcinoma

... Presenter : Peng-Ming Chen                Commentator : Dr. Wu Chou Su            ...
Slide 1
Slide 1

... FIGURE 2.7 Disposition and structure of the Golgi lysosome system in mammalian oocytes of the mouse (A–C), human (D), and macaque (E). (A) illustrates the lysosome pattern revealed after staining with the lysosome membrane–specific antibody LAMP-2; small particles represent primary lysosomes, where ...
D.J. Perreault and J.G. Kassakian, Analysis and Control of a Cellular Converter System With Stochastic Ripple Cancellation and Minimal Magnetics, IEEE Transactions on Power Electronics , Vol. 12, No. 1, Jan. 1997, pp. 145-152.
D.J. Perreault and J.G. Kassakian, Analysis and Control of a Cellular Converter System With Stochastic Ripple Cancellation and Minimal Magnetics, IEEE Transactions on Power Electronics , Vol. 12, No. 1, Jan. 1997, pp. 145-152.

... for equivalent PRPI converters of 2–15 cells. To model the parameter variations occurring in real converters, a uniformly distributed 5% random variation in resonant component values was used. The results of these simulations, plotted in Fig. 10, corroborate the analysis. With slight modifications t ...
Neural Responses to Taxation and Voluntary Giving Reveal Motives
Neural Responses to Taxation and Voluntary Giving Reveal Motives

Persistent Firing Supported by an Intrinsic Cellular
Persistent Firing Supported by an Intrinsic Cellular

... The rat postsubiculum has head direction cells that fire persistently when the rat’s head is oriented in particular directions. This persistent firing is maintained even if the rat is motionless, when spatial cues are removed from the environment and in the dark, but the mechanism that supports pers ...
Canadian Journal of Microbiology 47
Canadian Journal of Microbiology 47

... When immobilized in alginate beads and applied experimentally in semiarid agriculture (Bashan 1998; Bashan et al. 1987), the bacterium A. brasilense has been found to survive for prolonged periods (Bashan and Gonzalez 1999). As a seed or root inoculant, diazotrophic A. brasilense nonspecifically pro ...
Noll et al., 2007  - Institute of Molecular Life Sciences
Noll et al., 2007 - Institute of Molecular Life Sciences

... positions corresponding to the iron-binding ligand amino acids in the diferric transferrins are marked by asterisks. The signal sequence is underlined, and potential glycosylation sites are marked by solid circles for the strong NXT type and dotted circles for the weak NXS type. The GenBank accessio ...
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Amitosis

Amitosis (a- + mitosis) is absence of mitosis, the usual form of cell division in the cells of eukaryotes. There are several senses in which eukaryotic cells can be amitotic. One refers to capability for non-mitotic division and the other refers to lack of capability for division. In one sense of the word, which is now mostly obsolete, amitosis is cell division in eukaryotic cells that happens without the usual features of mitosis as seen on microscopy, namely, without nuclear envelope breakdown and without formation of mitotic spindle and condensed chromosomes as far as microscopy can detect. However, most examples of cell division formerly thought to belong to this supposedly ""non-mitotic"" class, such as the division of unicellular eukaryotes, are today recognized as belonging to a class of mitosis called closed mitosis. A spectrum of mitotic activity can be categorized as open, semi-closed, and closed mitosis, depending on the fate of the nuclear envelope. An exception is the division of ciliate macronucleus, which is not mitotic, and the reference to this process as amitosis may be the only legitimate use of the ""non-mitotic division"" sense of the term today. In animals and plants which normally have open mitosis, the microscopic picture described in the 19th century as amitosis most likely corresponded to apoptosis, a process of programmed cell death associated with fragmentation of the nucleus and cytoplasm. Relatedly, even in the late 19th century cytologists mentioned that in larger life forms, amitosis is a ""forerunner of degeneration"".Another sense of amitotic refers to cells of certain tissues that are usually no longer capable of mitosis once the organism has matured into adulthood. In humans this is true of various muscle and nerve tissue types; if the existing ones are damaged, they cannot be replaced with new ones of equal capability. For example, cardiac muscle destroyed by heart attack and nerves destroyed by piercing trauma usually cannot regenerate. In contrast, skin cells are capable of mitosis throughout adulthood; old skin cells that die and slough off are replaced with new ones. Human liver tissue also has a sort of dormant regenerative ability; it is usually not needed or expressed but can be elicited if needed.
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