Tansley review - Professor Gero Steinberg
... et al., 2006). Thus, MT motors, and in particular kinesins, participate in a broad spectrum of functions in the fungal cell, including membrane transport, spindle elongation in mitosis and regulation of MT dynamics. However, our knowledge of the role of MT-based motors in fungi has been restricted m ...
... et al., 2006). Thus, MT motors, and in particular kinesins, participate in a broad spectrum of functions in the fungal cell, including membrane transport, spindle elongation in mitosis and regulation of MT dynamics. However, our knowledge of the role of MT-based motors in fungi has been restricted m ...
Signal Perception and Transduction: The Origin of
... similar, apparently unresolved problems. Experimental studies on Mougeofia used plane-polarized red light to demonstrate that the form of phytochrome controlling chloroplast rotation is attached to some fixed cellular structure, most likely the plasma membrane. Nevertheless, activated phytochrome ca ...
... similar, apparently unresolved problems. Experimental studies on Mougeofia used plane-polarized red light to demonstrate that the form of phytochrome controlling chloroplast rotation is attached to some fixed cellular structure, most likely the plasma membrane. Nevertheless, activated phytochrome ca ...
Germ Cell Speci fi cation - Molecular Biology and Genetics
... Several lines of evidence suggest that C. elegans embryos possess germ plasm. As described above, the germline-specific P granules and associated RNAs and RNAbinding proteins co-segregate to the same side of the P blastomere before each asymmetric cleavage (Table 2.1). P or “germ” granules have been ...
... Several lines of evidence suggest that C. elegans embryos possess germ plasm. As described above, the germline-specific P granules and associated RNAs and RNAbinding proteins co-segregate to the same side of the P blastomere before each asymmetric cleavage (Table 2.1). P or “germ” granules have been ...
Full Text
... Many chromatin regulators are subunits within large complexes, yet there seems to be little agreement on what the term “complex” implies and the difference between a “subunit” and an “associated protein.” In this review, we use the term “subunit” to imply two critical features. The first is stabilit ...
... Many chromatin regulators are subunits within large complexes, yet there seems to be little agreement on what the term “complex” implies and the difference between a “subunit” and an “associated protein.” In this review, we use the term “subunit” to imply two critical features. The first is stabilit ...
Immune responses in mice vaccinated with a DNA vaccine
... was cloned into pET28a expression vector using the T7 promoter. The recombinant plasmid pET28a/ Ts-NBLsp was determined by DNA sequencing and transformed in Escherichia coli BL21 (DE3) chemically competent cells. The BL21 cells containing pET28a/Ts-NBLsp grown in 1 L LB and were shaken vigorously of ...
... was cloned into pET28a expression vector using the T7 promoter. The recombinant plasmid pET28a/ Ts-NBLsp was determined by DNA sequencing and transformed in Escherichia coli BL21 (DE3) chemically competent cells. The BL21 cells containing pET28a/Ts-NBLsp grown in 1 L LB and were shaken vigorously of ...
Defining the Three Cell Lineages of the Human Blastocyst by Single
... To further understand developmental gene expression dynamics, we used k-means clustering to group genes with similar expression profiles in the human and mouse time-course data across development (Fig. 1B, Figs S1, S2 and Tables S1, S2). We focused our analysis on genes with a fold change of more th ...
... To further understand developmental gene expression dynamics, we used k-means clustering to group genes with similar expression profiles in the human and mouse time-course data across development (Fig. 1B, Figs S1, S2 and Tables S1, S2). We focused our analysis on genes with a fold change of more th ...
Plant Cell
... et al., 2014). In support of this hypothesis is a recent study demonstrating that the calcium dependent protein kinase 5 (CPK5) is important for the propagation of the ROS wave in plants (Romeis and Herde, 2014). ROS generated in the apoplast by an RBOHD protein of a neighboring cell (as part of the ...
... et al., 2014). In support of this hypothesis is a recent study demonstrating that the calcium dependent protein kinase 5 (CPK5) is important for the propagation of the ROS wave in plants (Romeis and Herde, 2014). ROS generated in the apoplast by an RBOHD protein of a neighboring cell (as part of the ...
Morphology and LPS content for the estimation of marine
... using two different methods: the epifluorescent microscopy technique for enumerating and sizing bacterial cells, and the determination of bacterial lipopolysaccharides (LPS). Five bacterial morphotypes were distinguished: cocci, rods, coccobacilli, vibrios and spirillae. The proportions of cocci wer ...
... using two different methods: the epifluorescent microscopy technique for enumerating and sizing bacterial cells, and the determination of bacterial lipopolysaccharides (LPS). Five bacterial morphotypes were distinguished: cocci, rods, coccobacilli, vibrios and spirillae. The proportions of cocci wer ...
PDF
... skin (14, 15, 62–64). Despite so, bath vaccination studies have shown that the primary site of attachment of V. anguillarum is the skin in which the bacteria cause local inflammatory responses upon attachment (65, 66). This causes the skin epithelial cells to phagocytose pathogens that attach to the ...
... skin (14, 15, 62–64). Despite so, bath vaccination studies have shown that the primary site of attachment of V. anguillarum is the skin in which the bacteria cause local inflammatory responses upon attachment (65, 66). This causes the skin epithelial cells to phagocytose pathogens that attach to the ...
PDF
... with cut-off E = 1.0 e−5 (Pérez-Rodríguez et al., 2009). GO classification is a way of unifying functional characteristics of all biological systems. Based on GO classification (http://www. geneontology.org), assembled transcripts were classified into three categories, namely biological process, mol ...
... with cut-off E = 1.0 e−5 (Pérez-Rodríguez et al., 2009). GO classification is a way of unifying functional characteristics of all biological systems. Based on GO classification (http://www. geneontology.org), assembled transcripts were classified into three categories, namely biological process, mol ...
Connective Tissue4_Cartilage and Bone
... i. darker staining; basophilia due to sulphur groups and acidic groups c. Interterritorial matrix stains less intensely 3. Lacunae a. An artifact of fixation leaves spaces where chondrocytes reside 4. Avascular a. nutrients must diffuse into cartilage B. CELLS OF CARTILAGE 1. Chondroblast a. Cartila ...
... i. darker staining; basophilia due to sulphur groups and acidic groups c. Interterritorial matrix stains less intensely 3. Lacunae a. An artifact of fixation leaves spaces where chondrocytes reside 4. Avascular a. nutrients must diffuse into cartilage B. CELLS OF CARTILAGE 1. Chondroblast a. Cartila ...
Keratin Alterations during Embryonic Epidermal Differentiation: A
... Differentiation of the epidermis during embryonic rabbit development was found to be accompanied by dramatic changes in keratin proteins . Immunofluorescent labeling with keratin antiserum revealed that the undifferentiated epithelium of 12-d embryos was already committed to making keratin proteins ...
... Differentiation of the epidermis during embryonic rabbit development was found to be accompanied by dramatic changes in keratin proteins . Immunofluorescent labeling with keratin antiserum revealed that the undifferentiated epithelium of 12-d embryos was already committed to making keratin proteins ...
pdf: Xu et al. 2008
... The author responsible for distribution of materials integral to the findings presented in this article in accordance with the policy described in the Instructions for Authors (www.plantcell.org) is: Joseph J. Kieber ([email protected]). W Online version contains Web-only data. www.plantcell.org/cgi/d ...
... The author responsible for distribution of materials integral to the findings presented in this article in accordance with the policy described in the Instructions for Authors (www.plantcell.org) is: Joseph J. Kieber ([email protected]). W Online version contains Web-only data. www.plantcell.org/cgi/d ...
The Peter Doherty Institute for Infection and Immunity Department of
... microbes that normally colonise our body surfaces (microbiota). However, precisely how these microorganisms and the resulting stimulation of the immune system impact on T cells still remains elusive. Building on exciting new findings in the lab, this project will investigate how the microbiota influ ...
... microbes that normally colonise our body surfaces (microbiota). However, precisely how these microorganisms and the resulting stimulation of the immune system impact on T cells still remains elusive. Building on exciting new findings in the lab, this project will investigate how the microbiota influ ...
Individual-based modelling of growth and migration of Salmonella
... yolk (Gast et al., 2002), particularly its membrane (Gast and Holt, 2001b). Upon horizontal transmission, the organism crosses the shell and membranes and gains access to the albumen. The bacteria that succeed to migrate to the yolk encounter a growth-friendly environment, whereas albumen evolved to ...
... yolk (Gast et al., 2002), particularly its membrane (Gast and Holt, 2001b). Upon horizontal transmission, the organism crosses the shell and membranes and gains access to the albumen. The bacteria that succeed to migrate to the yolk encounter a growth-friendly environment, whereas albumen evolved to ...
Cell-cycle-regulated expression of STIL controls centriole number in
... marker for centrioles and CP110 was used to time the emergence of newly forming centrioles during S phase (Chen et al., 2002; Kleylein-Sohn et al., 2007). In parallel, DNA was stained with DAPI to distinguish between interphase and mitotic cells and to identify different mitotic stages. Of the G1 ce ...
... marker for centrioles and CP110 was used to time the emergence of newly forming centrioles during S phase (Chen et al., 2002; Kleylein-Sohn et al., 2007). In parallel, DNA was stained with DAPI to distinguish between interphase and mitotic cells and to identify different mitotic stages. Of the G1 ce ...
Genetic Control of Fusion Pore Expansion in the Epidermis of
... and recognition and adhesion between myoblasts (Doberstein et al., 1997; Abmayr et al., 2003; Chen et al., 2003). However, genes necessary and sufficient for the actual merger of two plasma membranes into one have not been reported in other developmental cell fusion reactions outside syncytin-mediat ...
... and recognition and adhesion between myoblasts (Doberstein et al., 1997; Abmayr et al., 2003; Chen et al., 2003). However, genes necessary and sufficient for the actual merger of two plasma membranes into one have not been reported in other developmental cell fusion reactions outside syncytin-mediat ...
Hedgehog signaling and yolk sac angiogenesis
... islands (arrows, Fig. 1A). The position of the Ptch-expressing cells in a layer surrounding the blood island suggests that they could be endothelial, mesothelial or perhaps vascular smooth muscle cells. We therefore examined the expression of molecular markers for these cell types in both R1 Ptch-la ...
... islands (arrows, Fig. 1A). The position of the Ptch-expressing cells in a layer surrounding the blood island suggests that they could be endothelial, mesothelial or perhaps vascular smooth muscle cells. We therefore examined the expression of molecular markers for these cell types in both R1 Ptch-la ...
Molecular - College of Biological Sciences
... centrosomes, called centrosome reduplication. Cdk2 activity protects the Mps1 protein kinase from proteasome-mediated degradation, and we demonstrate here that Mps1 mediates cyclin A-dependent centrosome reduplication. Overexpression of cyclin A or a brief proteasome inhibition increases the centros ...
... centrosomes, called centrosome reduplication. Cdk2 activity protects the Mps1 protein kinase from proteasome-mediated degradation, and we demonstrate here that Mps1 mediates cyclin A-dependent centrosome reduplication. Overexpression of cyclin A or a brief proteasome inhibition increases the centros ...
Cell size trade-offs govern light exploitation strategies in
... energy for metabolism, k is the Boltzmann’s constant (8.62 eV K-1), T is temperature and b is the size scaling exponent, measured as the log-log slope of metabolic rate per cell as a function of cell volume (Kleiber, 1947; Hemmingsen, 1960; Peters, 1983; Gillooly et al., 2001). The size scaling expo ...
... energy for metabolism, k is the Boltzmann’s constant (8.62 eV K-1), T is temperature and b is the size scaling exponent, measured as the log-log slope of metabolic rate per cell as a function of cell volume (Kleiber, 1947; Hemmingsen, 1960; Peters, 1983; Gillooly et al., 2001). The size scaling expo ...
Hormonally Regulated Programmed Cell Death in
... after the aleurone cell died, “highly refractive globules” accumulated within the walls. Vacuolation of aleurone cells after gibberellin (GA) treatment has since been described for barley (Jones and Price, 1970; Bush et al., 1986), wheat (Kuo et al., 1996), and wild oat (Hooley, 1982). Haberlandt (1 ...
... after the aleurone cell died, “highly refractive globules” accumulated within the walls. Vacuolation of aleurone cells after gibberellin (GA) treatment has since been described for barley (Jones and Price, 1970; Bush et al., 1986), wheat (Kuo et al., 1996), and wild oat (Hooley, 1982). Haberlandt (1 ...
Tuberculosis vaccines – rethinking the current paradigm
... on vaccination strategies TB can be broadly characterized as primary disease (following a recent exposure) or LTBI, which is clinically defined as an individual demonstrating pre-existing cellular memory against MTB antigens (e.g., via a positive tuberculin skin test) but without clinical disease sy ...
... on vaccination strategies TB can be broadly characterized as primary disease (following a recent exposure) or LTBI, which is clinically defined as an individual demonstrating pre-existing cellular memory against MTB antigens (e.g., via a positive tuberculin skin test) but without clinical disease sy ...