Different strategies of osmoadaptation in the closely
... with 9-fluorenylmethoxycarbonyl (FMOC) by adding 40 ml boric acid buffer (0.5 M boric acid and 25 mM norvalin) and 80 ml FMOC reagent (1 mM FMOC in acetone) to 40 ml 1:50-diluted sample material. After shaking for 45 s, 100 ml 1-aminoadamantane (ADAM) reagent (50 % 40 mM ADAM in boric acid buffer, 5 ...
... with 9-fluorenylmethoxycarbonyl (FMOC) by adding 40 ml boric acid buffer (0.5 M boric acid and 25 mM norvalin) and 80 ml FMOC reagent (1 mM FMOC in acetone) to 40 ml 1:50-diluted sample material. After shaking for 45 s, 100 ml 1-aminoadamantane (ADAM) reagent (50 % 40 mM ADAM in boric acid buffer, 5 ...
Status of the p53, p16, RB1, and HER
... difference between cancer specimens and the distal mucosa with regard to p53 deletion. The presence of allelic losses of the TP53 markers in cystectomy specimens and in areas of urothelium that are considered benign has been demonstrated by conventional histology.11 12 These data, together with the ...
... difference between cancer specimens and the distal mucosa with regard to p53 deletion. The presence of allelic losses of the TP53 markers in cystectomy specimens and in areas of urothelium that are considered benign has been demonstrated by conventional histology.11 12 These data, together with the ...
Original Article:
... In the present study we have developed a method, spa-DGGE, to simultaneously detect multiple carriage and/or infection of S. aureus strains. Previous studies addressing this question are biased due to using conventional cultivation of one or a limited number of S. aureus colonies picked from agar pl ...
... In the present study we have developed a method, spa-DGGE, to simultaneously detect multiple carriage and/or infection of S. aureus strains. Previous studies addressing this question are biased due to using conventional cultivation of one or a limited number of S. aureus colonies picked from agar pl ...
Familial nonrandom inactivation linked to the X inactivation
... out subsequent cell divisions and, by this process, the dosage of proteins encoded by genes on the X chromosome is equalised in males and females. The choice of which of the two X chromosomes is the active one is usually random. Although stochastic events may account for a less than equal distributi ...
... out subsequent cell divisions and, by this process, the dosage of proteins encoded by genes on the X chromosome is equalised in males and females. The choice of which of the two X chromosomes is the active one is usually random. Although stochastic events may account for a less than equal distributi ...
Lab 7. Mendelian Genetics
... of unrelated species—it easy to lose sight of the basics of the process that makes it all possible. Depicting genetic make–up Genes control the characteristics of an organism. Alleles are alternate forms of a gene. For example, there is an allele for blond hair, another for black hair, etc. Only two ...
... of unrelated species—it easy to lose sight of the basics of the process that makes it all possible. Depicting genetic make–up Genes control the characteristics of an organism. Alleles are alternate forms of a gene. For example, there is an allele for blond hair, another for black hair, etc. Only two ...
Genome-Wide Identification of Allelic Expression in Hypertensive Rats
... Genomic SSLP data were available for 2 SHR lines (SHR/OlaHsd and SHRSP/Riv) and 1 WKY line (WKY/OlaHsd). These lines are closely related to, and may be identical to, the SHR/NCrl, SHR-A3, and WKY/NCrl lines, respectively, used in our studies. We used this data to assess the degree of allelic identit ...
... Genomic SSLP data were available for 2 SHR lines (SHR/OlaHsd and SHRSP/Riv) and 1 WKY line (WKY/OlaHsd). These lines are closely related to, and may be identical to, the SHR/NCrl, SHR-A3, and WKY/NCrl lines, respectively, used in our studies. We used this data to assess the degree of allelic identit ...
Classifying Gene Expression Data using an Evolutionary Algorithm
... be not differential expressed. There are also other estimation methods for the constant c that have been proposed. At the next level of complication, the location normalization method is often necessary to allow the correction c to vary between spots in an intensity-dependent manner. The log-ratios ...
... be not differential expressed. There are also other estimation methods for the constant c that have been proposed. At the next level of complication, the location normalization method is often necessary to allow the correction c to vary between spots in an intensity-dependent manner. The log-ratios ...
PDF - Molecular Cytogenetics
... of mosaicism occur as genetically altered clonal lineages that can arise following mitotic errors in normal cells. In addition there are also cases where a new lineage emerged from multiple trisomic or monosomic rescue events in altered cells [14–17]. The possibility of independent rescue events occ ...
... of mosaicism occur as genetically altered clonal lineages that can arise following mitotic errors in normal cells. In addition there are also cases where a new lineage emerged from multiple trisomic or monosomic rescue events in altered cells [14–17]. The possibility of independent rescue events occ ...
Nucleotide sequence and genome organization of foot-and
... about 8500 nucleotides, i.e. 500 nucleotides longer than estimated from sizinggets by (17). This size correction is also supported by the sizing and the partial sequence analysis of cDNA copies that cover the as yet uncloned 5'-terminat part of the FMDV genome, i.e. the S segment and the poly(C) tra ...
... about 8500 nucleotides, i.e. 500 nucleotides longer than estimated from sizinggets by (17). This size correction is also supported by the sizing and the partial sequence analysis of cDNA copies that cover the as yet uncloned 5'-terminat part of the FMDV genome, i.e. the S segment and the poly(C) tra ...
Y chromosome azoospermia factor region microdeletions and
... kb [26] and it is specifically expressed in testis tissue [27]. This gene encodes an ATP dependent RNA helicase DEAD box protein in humans and plays a significant role in the pre-meiotic spermatogonia phase of spermatogenesis [27]. Molecular analysis revealed a high prevalence of deletions or mutati ...
... kb [26] and it is specifically expressed in testis tissue [27]. This gene encodes an ATP dependent RNA helicase DEAD box protein in humans and plays a significant role in the pre-meiotic spermatogonia phase of spermatogenesis [27]. Molecular analysis revealed a high prevalence of deletions or mutati ...
Slide 1
... But if a homozygote recessive eats a diet low in phenylalanine, no negative consequences develop. So, the genetic predisposition to express the disorder is influenced by the environment. ...
... But if a homozygote recessive eats a diet low in phenylalanine, no negative consequences develop. So, the genetic predisposition to express the disorder is influenced by the environment. ...
A novel arginine substitution mutation in 1A domain and a novel 27
... region have been reported.26–29 The proband of family B carries a novel duplication insertion of 27 nucleotides (1222ins27). This type of mutation is unique for a number of different reasons. Firstly, in-frame insertion or deletion mutations are extremely rare in keratin diseases (table 2), and this ...
... region have been reported.26–29 The proband of family B carries a novel duplication insertion of 27 nucleotides (1222ins27). This type of mutation is unique for a number of different reasons. Firstly, in-frame insertion or deletion mutations are extremely rare in keratin diseases (table 2), and this ...
Loss-of-function of a Rice Gibberellin Biosynthetic Gene, GA20
... et al. 1999, Ueguchi-Tanaka et al. 2000, Ikeda et al. 2001, Itoh et al. 2001, Itoh et al. 2002). Through these studies, we recognized that the plant morphology of the sd1 mutants was similar to that of GA-deficient mutants with a weak phenotype. To determine whether the SD1 gene might be related to ...
... et al. 1999, Ueguchi-Tanaka et al. 2000, Ikeda et al. 2001, Itoh et al. 2001, Itoh et al. 2002). Through these studies, we recognized that the plant morphology of the sd1 mutants was similar to that of GA-deficient mutants with a weak phenotype. To determine whether the SD1 gene might be related to ...
Recommended Nomenclature for the Vertebrate Alcohol
... named ADH1. For multiple isoenzymes within a class, we recommend adding a capital letter after the Arabic number rather than Greek symbols. Thus, the three human class I ADHs previously called ADHa, ADHb, and ADHg are named ADH1A, ADH1B, and ADH1C, respectively. For genes, we recommend the italicize ...
... named ADH1. For multiple isoenzymes within a class, we recommend adding a capital letter after the Arabic number rather than Greek symbols. Thus, the three human class I ADHs previously called ADHa, ADHb, and ADHg are named ADH1A, ADH1B, and ADH1C, respectively. For genes, we recommend the italicize ...
6.3 Mendel and Heredity
... • Gregor Mendel showed that traits are inherited as discrete units. – Like different colored marbles mixed together that can still be picked out separately. ...
... • Gregor Mendel showed that traits are inherited as discrete units. – Like different colored marbles mixed together that can still be picked out separately. ...
NUCLEIC ACID ECONOMY IN BACTERIA INFECTED WITH
... 0.1 gin. gelatin, and HC1 to adjust to pH 7.4. Analytical Methods.--Most of the experiments required periodic measures of DNAP~ and mature phage P~. DNA-P 3~ was determined (Schmidt and Thannhauser, 1945) by precipitating a chilled 1 ml. sample of the culture in a conical tube with 9 ml. 0.3 M trich ...
... 0.1 gin. gelatin, and HC1 to adjust to pH 7.4. Analytical Methods.--Most of the experiments required periodic measures of DNAP~ and mature phage P~. DNA-P 3~ was determined (Schmidt and Thannhauser, 1945) by precipitating a chilled 1 ml. sample of the culture in a conical tube with 9 ml. 0.3 M trich ...
msb201035-sup
... development and have a CRM bound at either early (green) or late (red) stages of development. (a) The correlation between the timing of each TF separately to the timing of its target genes expression: the target genes for each TF were divided into early or late based on the timing of TF occupancy to ...
... development and have a CRM bound at either early (green) or late (red) stages of development. (a) The correlation between the timing of each TF separately to the timing of its target genes expression: the target genes for each TF were divided into early or late based on the timing of TF occupancy to ...
Visualizing expression patterns of Shh and Foxf1 genes
... Recently, various tools for obtaining three-dimensional (3D) information on biological tissues have been investigated and improved [17–19]. Optical projection tomography (OPT) is a new, rapid and non-invasive technique for 3D imaging of small biological specimens that allows visualization of the tis ...
... Recently, various tools for obtaining three-dimensional (3D) information on biological tissues have been investigated and improved [17–19]. Optical projection tomography (OPT) is a new, rapid and non-invasive technique for 3D imaging of small biological specimens that allows visualization of the tis ...
Identification of Genetic and Epigenetic Risk Factors for Psoriasis
... indicating that other genetic effects and environmental triggers are important. In order to identify novel susceptibility genes of PS and PsA, I performed the first large scale genome wide association scan for psoriasis susceptibility loci using 233 cases and 519 controls. It revealed that genes of ...
... indicating that other genetic effects and environmental triggers are important. In order to identify novel susceptibility genes of PS and PsA, I performed the first large scale genome wide association scan for psoriasis susceptibility loci using 233 cases and 519 controls. It revealed that genes of ...
Germline stem cells are critical for sexual fate decision of germ cells
... is conserved among vertebrates, many animals do not possess an Sry homolog. Ten years after the discovery of Sry, DMY/Dmrt1bY was identified as the sex determination gene on the sex chromosome in the teleost fish, medaka [5, 6]. Since this discovery, other sex determination genes have also been iden ...
... is conserved among vertebrates, many animals do not possess an Sry homolog. Ten years after the discovery of Sry, DMY/Dmrt1bY was identified as the sex determination gene on the sex chromosome in the teleost fish, medaka [5, 6]. Since this discovery, other sex determination genes have also been iden ...
PDF
... loss of the notochord. The Sd gene is known to interact with undulated (un), a sclerotome mutant. Double mutants between Sd and un alleles show an increase in the severity of the defects, mainly in the ventral parts of the vertebrae. We also show that part of the Sd phenotype is strikingly similar t ...
... loss of the notochord. The Sd gene is known to interact with undulated (un), a sclerotome mutant. Double mutants between Sd and un alleles show an increase in the severity of the defects, mainly in the ventral parts of the vertebrae. We also show that part of the Sd phenotype is strikingly similar t ...
Spontaneous Mutations in the CsrRS Two
... of the plasmid. Isolates obtained from invasive streptococcal infection of humans also were screened by plasmid complementation and curing, to determine whether they had a csrRS-repressible phenotype. All strains were compared on media incubated in air and in 5% CO2. We estimated the approximate cop ...
... of the plasmid. Isolates obtained from invasive streptococcal infection of humans also were screened by plasmid complementation and curing, to determine whether they had a csrRS-repressible phenotype. All strains were compared on media incubated in air and in 5% CO2. We estimated the approximate cop ...
Application for rDNA Review/Registration for
... 3. Is drug resistance expressed? A. If yes, indicate to which drugs: ...
... 3. Is drug resistance expressed? A. If yes, indicate to which drugs: ...
Käfer, E. and D. Luk
... in stock strains (information about similar observations would be helpful for the assessment and investigation of this intriguing observation). ...
... in stock strains (information about similar observations would be helpful for the assessment and investigation of this intriguing observation). ...
Site-specific recombinase technology
Nearly every human gene has a counterpart in the mouse (regardless of the fact that a minor set of orthologues had to follow species specific selection routes). This made the mouse the major model for elucidating the ways in which our genetic material encodes information. In the late 1980s gene targeting in murine embryonic stem (ES-)cells enabled the transmission of mutations into the mouse germ line and emerged as a novel option to study the genetic basis of regulatory networks as they exist in the genome. Still, classical gene targeting proved to be limited in several ways as gene functions became irreversibly destroyed by the marker gene that had to be introduced for selecting recombinant ES cells. These early steps led to animals in which the mutation was present in all cells of the body from the beginning leading to complex phenotypes and/or early lethality. There was a clear need for methods to restrict these mutations to specific points in development and specific cell types. This dream became reality when groups in the USA were able to introduce bacteriophage and yeast-derived site-specific recombination (SSR-) systems into mammalian cells as well as into the mouse