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Antibiotics involved in Clostridium difficile
Antibiotics involved in Clostridium difficile

... genes encoding known, or putative, colonization factors: three adhesins, P47 (one of the two Slayer proteins), Cwp66 and Fbp68, and a protease, Cwp84. The conditions studied included hyperosmolarity, iron depletion and exposure to several antibiotics (ampicillin, clindamycin, ofloxacin, moxifloxacin ...
Origin and evolution of Y chromosomes: Drosophila tales
Origin and evolution of Y chromosomes: Drosophila tales

... suggests an upper bound of 20 genes [21–24]. These genes are unusually large, owing to Mbp-sized introns comprising repetitive DNA [25]. D. melanogaster Y-linked genes have two additional important features; many (and probably all) have male-related functions (e.g. encoding sperm flagella motor pro ...
The Genetic Basis of Melanism in the Gray Squirrel (Sciurus
The Genetic Basis of Melanism in the Gray Squirrel (Sciurus

... into the wild and has subsequently become a successful invader all but outcompeting the native red squirrel (Sciurus vulgaris). Melanic variants of the gray squirrel are common in North America, but the first sighting reported in Britain was in the early 20th century. These black squirrels are now a ...
Käfer, E. and D.  Luk
Käfer, E. and D. Luk

... in stock strains (information about similar observations would be helpful for the assessment and investigation of this intriguing observation). ...
Gene Section CASP8 (Caspase 8, Apoptosis-Related Cysteine Peptidase) Atlas of Genetics and Cytogenetics
Gene Section CASP8 (Caspase 8, Apoptosis-Related Cysteine Peptidase) Atlas of Genetics and Cytogenetics

MUTATION STUDIES AT THE A, LOCUS IN MAIZE. I. A
MUTATION STUDIES AT THE A, LOCUS IN MAIZE. I. A

... (1948) the A , allele found in North American races was shown to be necessary for the production of anthocyanin pigments in various tissues of the corn plant. With the complementary genes A,, B, Pl, and R' or r', A , produces a deep purple pigmentation of the sheath, brace roots, auricle, leaf margi ...
Variable Autosomal and X Divergence Near and Far from Genes
Variable Autosomal and X Divergence Near and Far from Genes

... nearest gene for ten great ape populations (fig. 1). We found that divergence in putatively neutral regions increased as the distance from genes increased on both the X and the autosomes (fig. 1). We observed that divergence estimates exhibit a similar pattern along the genome for all great ape subs ...
File - Dr. Spence - Advanced Placement Biology
File - Dr. Spence - Advanced Placement Biology

... encoded by the mother’s genes, that influence development. Many of these cytoplasmic determinants, like the two shown here, are unevenly distributed in the egg. After fertilization and mitotic division, the cell nuclei of the embryo are exposed to different sets of cytoplasmic determinants and, as a ...
Chromosomal Microarray Analysis
Chromosomal Microarray Analysis

... Chromosomal Microarray Analysis revealed an approximately 28.2 Mb LOSS in copy number in the distal and subtelomeric regions of the long arm of chromosome 18 suggestive of mosaicism. This deletion includes the critical region of chromosome 18q deletion syndrome (OMIM 601808). FISH analysis and parti ...
Hemoglobin A2: origin, evolution, and aftermath
Hemoglobin A2: origin, evolution, and aftermath

... of maturity. These experiments showed that there was a progressive decrease in &-globin synthesis in relation to P-globin in increasingly mature cells. A relative instability of 8-globin mRNA was proposed as a mechanism for the premature decrease in &globin synthesis? Using highly selective probes f ...
The distribution of DNA translocation times in solid
The distribution of DNA translocation times in solid

... chain length on the distribution of DNA translocation times through 8 ± 2 nm diameter silicon nitride nanopores. Linear dsDNA translocation events were selected based on the magnitude of current blockage and accumulated into scatter plots of current blockage and event duration (translocation time). ...
1 - SACE
1 - SACE

... in agriculture and horticulture from different sources. Manipulates apparatus, equipment, and technological tools carefully and mostly effectively to implement organised safe and ethical investigation procedures. Obtains, records, and displays findings of investigations using appropriate conventions ...
Synthetic Chemical Inducers and Genetic
Synthetic Chemical Inducers and Genetic

... Figure 4. Design and evaluation of improved, rhaS-encoding reporter plasmid, pCK302. (a) Design of pCK302. It was hypothesized that weak induction of PrhaBAD resulted from poor rhaS expression, which in turn was due to suboptimum interaction of candidate inducers with RhaR. A new reporter plasmid, p ...
Supporting Information Legends
Supporting Information Legends

... (black bold line) and gene body of LOC_Os04g59624 (thin line with blue and white boxes representing exons). The XbaI site that was artificially created in the 7th intron for genotyping is shown by an asterisk. tgENL1-Venus has a Venus-coding sequence (green box) that is fused to the coding sequence ...
Unit 4 - Purdue Agriculture
Unit 4 - Purdue Agriculture

... During the 1860s, an Austrian monk and botanist named Gregor Mendel discovered that “factors” determine inheritance in pea plants. He observed that many traits, from height to flower color, seemed to be controlled by these factors. In 1903, researcher William Sutton made the connection between Mende ...
Studies on the biotic Stress Resistance in Rice through Molecular
Studies on the biotic Stress Resistance in Rice through Molecular

... no additional cost to the farmer and are compatible with other control methods in an integrated pest control programmer. Resistance to stem borers appears to be under polygenic control. Many morphological, anatomical, physiological and biochemical factors have been reported to be associated with res ...
Amplification of AML1 on a duplicated chromosome 21 in
Amplification of AML1 on a duplicated chromosome 21 in

... 7), the finding of discrete signals with both probes in each patient suggested that the entire gene was amplified (Figures 1c and d). The approximate number of copies of AML1 signals is shown in Table 1. This number varied between patients and also from cell to cell within the same sample, the close ...
Module 1 - Bioinformatics.ca
Module 1 - Bioinformatics.ca

... – Genome may be constant but an experimental condition has a pronounced effect on gene expression • e.g. Drug treated vs. untreated cell line • e.g. Wild type versus knock out mice ...
Ch. 13 Meiosis - HobbsAPBiology
Ch. 13 Meiosis - HobbsAPBiology

... Protein zipper - synaptonemal complex 5. Chromosomes together as Tetrad 6. Crossing over - sections exchanges at junctions called Chiasmata ...
Mary Ann Osley*, Alastair Fleming, and Cheng
Mary Ann Osley*, Alastair Fleming, and Cheng

... role in histone ubiquitylation is also functionally conserved. Human PAF interacts with UbcH6 and RNF20/RNF40, forming a trimeric complex that monoubiquitylates H2B in vitro (Zhu et al., in press). In addition, the PAF complex in Arabadopsis has a role in regulating the levels of ub-H2B dependent H3 ...
Genetic Evidence for a Silent SUC Gene in Yeast.
Genetic Evidence for a Silent SUC Gene in Yeast.

... nature of their suco alleles or with respect to other genes affecting sucrose utilization, studies were carried out with strains having the genetic background of the S. cerevisiae laboratory strain S288C. S288C bears a single SUC+ allele, at the SUC2 locus, and has sueo alleles at all other SUC loci ...
Attack of the Superbugs
Attack of the Superbugs

... as ear infections, sinus infections, pneumonia, and meningitis. Before the introduction of penicillin in the early 1940s, a person might die from a pneumococcal infection. After the introduction of penicillin, pneumococcal infections were effectively treated. However, strains of pneumococcal bacteri ...
3.1 Intro to Genetics
3.1 Intro to Genetics

... By the end of this unit, you will understand how we inherit traits from our parents  If ...
The Mutant of sll1961, Which Encodes a Putative
The Mutant of sll1961, Which Encodes a Putative

... protein is regulated at transcriptional level, we performed real-time RT-PCR analysis of psaA and/or psaAB during acclimation to high light. Low-lightgrown cells were shifted to high light and time course of change in psaA and/or psaAB transcripts was examined (Fig. 5). The transcript level of psaA ...
Problem Sets - MIT Biology
Problem Sets - MIT Biology

... dominant phenotypes were used (and these cannot be used in complementation tests), or strains that were not true-breeding were used. Using strains that are not true-breeding is not the proper way to do a complementation test, because then multiple genotypes and phenotypes are produced instead of jus ...
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Site-specific recombinase technology



Nearly every human gene has a counterpart in the mouse (regardless of the fact that a minor set of orthologues had to follow species specific selection routes). This made the mouse the major model for elucidating the ways in which our genetic material encodes information. In the late 1980s gene targeting in murine embryonic stem (ES-)cells enabled the transmission of mutations into the mouse germ line and emerged as a novel option to study the genetic basis of regulatory networks as they exist in the genome. Still, classical gene targeting proved to be limited in several ways as gene functions became irreversibly destroyed by the marker gene that had to be introduced for selecting recombinant ES cells. These early steps led to animals in which the mutation was present in all cells of the body from the beginning leading to complex phenotypes and/or early lethality. There was a clear need for methods to restrict these mutations to specific points in development and specific cell types. This dream became reality when groups in the USA were able to introduce bacteriophage and yeast-derived site-specific recombination (SSR-) systems into mammalian cells as well as into the mouse
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