Real-time PCR-based detection of Bordetella pertussis and
... Novel real-time PCR assays targeting the Bordetella pertussis insertion sequence IS481, the toxin promoter region and Bordetella parapertussis insertion sequence IS1001 were designed. PCR assays were capable of detecting ¡10 copies of target DNA per reaction, with an amplification efficiency of ¢90 ...
... Novel real-time PCR assays targeting the Bordetella pertussis insertion sequence IS481, the toxin promoter region and Bordetella parapertussis insertion sequence IS1001 were designed. PCR assays were capable of detecting ¡10 copies of target DNA per reaction, with an amplification efficiency of ¢90 ...
General enquiries on this form should be made to
... species or transmission barrier effects, and the molecular basis of TSE strains. This project, SE1753, represents a Defra-funded research project aimed at the generation and assessment of transgenic PrP mouse models for the improved detection, differentiation and diagnosis of ruminant TSE agents. T ...
... species or transmission barrier effects, and the molecular basis of TSE strains. This project, SE1753, represents a Defra-funded research project aimed at the generation and assessment of transgenic PrP mouse models for the improved detection, differentiation and diagnosis of ruminant TSE agents. T ...
Designing synthetic MLPA probes - MRC
... targets on the sample DNA. 2. LPOs and RPOs that are hybridised to adjacent targets are ligated to form one complete probe. 3. During the PCR reaction, only complete probes are amplified exponentially. 4. The amplification products are separated by capillary electrophoresis. 5. Using intra- and inte ...
... targets on the sample DNA. 2. LPOs and RPOs that are hybridised to adjacent targets are ligated to form one complete probe. 3. During the PCR reaction, only complete probes are amplified exponentially. 4. The amplification products are separated by capillary electrophoresis. 5. Using intra- and inte ...
A General Model of Codon Bias Due to GC Mutational Bias
... we find additional mysteries. For many codons, usage is well modeled as a linear function of GC bias. For example, Figure 1B ...
... we find additional mysteries. For many codons, usage is well modeled as a linear function of GC bias. For example, Figure 1B ...
Seed Dormancy and Germination
... Isogenic Lines (NILs) (L. Bentsink, unpublished results). These NILs are being used to clone the QTL and also for further mutagenesis experiments that may lead to mutations in the respective QTL or in genes acting elsewhere in the dormancy/germination pathways. It is expected that the analysis of mo ...
... Isogenic Lines (NILs) (L. Bentsink, unpublished results). These NILs are being used to clone the QTL and also for further mutagenesis experiments that may lead to mutations in the respective QTL or in genes acting elsewhere in the dormancy/germination pathways. It is expected that the analysis of mo ...
Section 1 The application - Office of the Gene Technology Regulator
... The Gene Technology Act 2000 (the Act) and the Gene Technology Regulations 2001 (the Regulations) set out requirements which the Gene Technology Regulator (the Regulator) must follow when considering an application for a licence to intentionally release a genetically modified organism (GMO) into the ...
... The Gene Technology Act 2000 (the Act) and the Gene Technology Regulations 2001 (the Regulations) set out requirements which the Gene Technology Regulator (the Regulator) must follow when considering an application for a licence to intentionally release a genetically modified organism (GMO) into the ...
uncorrected page proofs
... A small plant (Colchicum autumnale) that grows across southern Europe has the common names meadow saffron, autumn crocus and naked lady. The name ‘naked lady’ is due to the fact that after the leaves of the plant appear in spring they die off, and the flowers appear in autumn on their own (see figure ...
... A small plant (Colchicum autumnale) that grows across southern Europe has the common names meadow saffron, autumn crocus and naked lady. The name ‘naked lady’ is due to the fact that after the leaves of the plant appear in spring they die off, and the flowers appear in autumn on their own (see figure ...
A role for wingless in the segmental gradient of Drosophila?
... for 2.5 hours at 25°C, then aged 13.5 hours at 15°C, given a heatshock pulse and left at 15°C for 4 days. Although the resulting smooth phenotypes are more variable than those of embryos kept at 15°C all the time, we did not observe consistent differences between embryos with or without the HSwg con ...
... for 2.5 hours at 25°C, then aged 13.5 hours at 15°C, given a heatshock pulse and left at 15°C for 4 days. Although the resulting smooth phenotypes are more variable than those of embryos kept at 15°C all the time, we did not observe consistent differences between embryos with or without the HSwg con ...
Polyploidy
... • Understand the differences between ‘Interference’, and the suppression of recombination resulting from inversions, • Be able to recognize data, and predict results given either case. ...
... • Understand the differences between ‘Interference’, and the suppression of recombination resulting from inversions, • Be able to recognize data, and predict results given either case. ...
Available as a free here - European Cystic Fibrosis Society
... Undoubtedly, there are positive and negative aspects of performing investigations to make a definitive diagnosis and the outcome is not always necessarily to the patient’s benefit. The implications of labelling patients with mild manifestations with a CF diagnosis include an implied clinical course an ...
... Undoubtedly, there are positive and negative aspects of performing investigations to make a definitive diagnosis and the outcome is not always necessarily to the patient’s benefit. The implications of labelling patients with mild manifestations with a CF diagnosis include an implied clinical course an ...
GENETIC AND MOLECULAR ANALYSIS OF THE garnet EYE
... Although any work of science depends on the prior work of others, the specific contributions of some must be mentioned. The garnet gene was cloned by Dr. D. Sinclair, working in the laboratory of Dr. G. Tener, biochemistry U.B.C. Dr. Sinclair initiated the molecular analysis of the garnet gene, brou ...
... Although any work of science depends on the prior work of others, the specific contributions of some must be mentioned. The garnet gene was cloned by Dr. D. Sinclair, working in the laboratory of Dr. G. Tener, biochemistry U.B.C. Dr. Sinclair initiated the molecular analysis of the garnet gene, brou ...
14 Chromosomes
... Some newborn babies have an abnormal number of chromosomes in their cells. A baby may have an additional chromosome, giving a total of 47 instead of the normal 46. One additional chromosome or one missing chromosome typically has deleterious effects on development and, for most chromosomes, death oc ...
... Some newborn babies have an abnormal number of chromosomes in their cells. A baby may have an additional chromosome, giving a total of 47 instead of the normal 46. One additional chromosome or one missing chromosome typically has deleterious effects on development and, for most chromosomes, death oc ...
Baker, B. S., Hoff, G., Kaufman, T. C., Wolfner, M. W., and Hazelrigg, T. (1991). A cytopgenetic analysis of the doublesex locus and its flanking regions. Genetics 127: 125-138.
... mapped and placed into complementation groups.Amongthemutations we isolated, four new mutations affecting sexual differentiation were identified. All proved to be alleles of dsx, suggesting that dsx is the only gene in this region involved in regulating sexual differentiation. All but one of the new ...
... mapped and placed into complementation groups.Amongthemutations we isolated, four new mutations affecting sexual differentiation were identified. All proved to be alleles of dsx, suggesting that dsx is the only gene in this region involved in regulating sexual differentiation. All but one of the new ...
Asymmetries in Retrieval of Gene Function Information
... The SI field identifies secondary source databanks and accession numbers of outside resources discussed in MEDLINE articles. The field is composed of the source followed by a slash followed by an accession number and can be searched with one or both components, e.g., genbank [si], AF001892 ...
... The SI field identifies secondary source databanks and accession numbers of outside resources discussed in MEDLINE articles. The field is composed of the source followed by a slash followed by an accession number and can be searched with one or both components, e.g., genbank [si], AF001892 ...
Familial Alzheimer`s Disease–Linked Presenilin 1 Variants Elevate
... studies indicate that conditioned medium from fibroblasts or plasma of affected members of pedigrees with PS1/PS2-linked mutations show a highly significant increase in the ratio of Ab1–42(43)/Ab1–40 relative to unaffected family members (Scheuner et al., 1996). The emerging view that Ab1–42(43) pla ...
... studies indicate that conditioned medium from fibroblasts or plasma of affected members of pedigrees with PS1/PS2-linked mutations show a highly significant increase in the ratio of Ab1–42(43)/Ab1–40 relative to unaffected family members (Scheuner et al., 1996). The emerging view that Ab1–42(43) pla ...
Document
... mutations in other ruminants, but also in other phylogenetic groups. The PrP gene is now one of the most sequenced genes. More than a hundred species have been analysed and in some species several hundred or even thousands of individual sequences have been recorded in research programmes. About 40 a ...
... mutations in other ruminants, but also in other phylogenetic groups. The PrP gene is now one of the most sequenced genes. More than a hundred species have been analysed and in some species several hundred or even thousands of individual sequences have been recorded in research programmes. About 40 a ...
PH4 of Petunia Is an R2R3 MYB Protein That Activates
... of the pathway, in all pigmented tissues (Quattrocchio et al., 1993) and encode a basic-helix-loop-helix (BHLH) transcription factor and a WD40 protein, respectively (de Vetten et al., 1997; Spelt et al., 2000). AN2 encodes a MYB-type transcription factor whose function appears to be (partially) red ...
... of the pathway, in all pigmented tissues (Quattrocchio et al., 1993) and encode a basic-helix-loop-helix (BHLH) transcription factor and a WD40 protein, respectively (de Vetten et al., 1997; Spelt et al., 2000). AN2 encodes a MYB-type transcription factor whose function appears to be (partially) red ...
Cotyledon organogenesis - Journal of Experimental Botany
... cotyledon mutants as tools for gene cloning (see Table 1 for a summary of cotyledon mutants in Arabidopsis and other species), many genetic pathways have been elucidated, which are usually included in reviews on embryonic patterning. However, one alternative consideration which provides a framework ...
... cotyledon mutants as tools for gene cloning (see Table 1 for a summary of cotyledon mutants in Arabidopsis and other species), many genetic pathways have been elucidated, which are usually included in reviews on embryonic patterning. However, one alternative consideration which provides a framework ...
Gregor Mendel was a 19th century priest and botanist who
... He articulated the laws of inheritance attributed to him insofar as was possible given the information he had.” -- [Fairbanks and Rytting (2001)]. Did Mendel detect but not mention linkage? o Mendel concluded "the behavior of each pair of differing traits in a hybrid association is independent of al ...
... He articulated the laws of inheritance attributed to him insofar as was possible given the information he had.” -- [Fairbanks and Rytting (2001)]. Did Mendel detect but not mention linkage? o Mendel concluded "the behavior of each pair of differing traits in a hybrid association is independent of al ...
wmgenes2 - Cucurbit Breeding
... they have dgdg genotype (Rhodes, 1986). The juvenile albino ja (Zhang et al., 1996b) gene causes reduced chlorophyll in seedling tissues, as well as leaf margins and fruit rind when plants are grown under short day conditions. The dominant gene Sp (Poole, 1944) causes round yellow spots to form on c ...
... they have dgdg genotype (Rhodes, 1986). The juvenile albino ja (Zhang et al., 1996b) gene causes reduced chlorophyll in seedling tissues, as well as leaf margins and fruit rind when plants are grown under short day conditions. The dominant gene Sp (Poole, 1944) causes round yellow spots to form on c ...
Publication - The University of Texas Health Science Center at
... general population is a first step in implicating potential genetic contributions. Robust estimates of prevalence and incidence rates of scleroderma, which are essential in determining familial aggregation, have varied widely in the general population, ranging from 3.1 to 20.8 per 100,000 and from 0. ...
... general population is a first step in implicating potential genetic contributions. Robust estimates of prevalence and incidence rates of scleroderma, which are essential in determining familial aggregation, have varied widely in the general population, ranging from 3.1 to 20.8 per 100,000 and from 0. ...
Site-specific recombinase technology
Nearly every human gene has a counterpart in the mouse (regardless of the fact that a minor set of orthologues had to follow species specific selection routes). This made the mouse the major model for elucidating the ways in which our genetic material encodes information. In the late 1980s gene targeting in murine embryonic stem (ES-)cells enabled the transmission of mutations into the mouse germ line and emerged as a novel option to study the genetic basis of regulatory networks as they exist in the genome. Still, classical gene targeting proved to be limited in several ways as gene functions became irreversibly destroyed by the marker gene that had to be introduced for selecting recombinant ES cells. These early steps led to animals in which the mutation was present in all cells of the body from the beginning leading to complex phenotypes and/or early lethality. There was a clear need for methods to restrict these mutations to specific points in development and specific cell types. This dream became reality when groups in the USA were able to introduce bacteriophage and yeast-derived site-specific recombination (SSR-) systems into mammalian cells as well as into the mouse