Document
... as question-asker, not just as infrastructure-builder or assistant to a biologist.” ...
... as question-asker, not just as infrastructure-builder or assistant to a biologist.” ...
study on factors affecting the efficiency of marker
... were feeded worldwide for their fast growing, high feed use efficiency, high muscle ratio and so on, while their meat quality is relatively poor, contrary, many native pig breeds of China possess good meat quality, but other traits are inferior. So if we discover some genes related to meat quality o ...
... were feeded worldwide for their fast growing, high feed use efficiency, high muscle ratio and so on, while their meat quality is relatively poor, contrary, many native pig breeds of China possess good meat quality, but other traits are inferior. So if we discover some genes related to meat quality o ...
Topologically Non-linked Circular Duplex DNA
... 1981). It was first observed in synthetic copolymers. It is not considered to be the structure of purified DNA in solution for any DNA obtained from natural sources, so that the presumption that DNA from natural sources can exist in the left-handed configuration is unproven. Nevertheless, we need to ...
... 1981). It was first observed in synthetic copolymers. It is not considered to be the structure of purified DNA in solution for any DNA obtained from natural sources, so that the presumption that DNA from natural sources can exist in the left-handed configuration is unproven. Nevertheless, we need to ...
Mechanisms of translational regulation in bacteria
... The conversion of genetic information stored in the DNA as a sequence of nucleotides into functional proteins. The synthesis of proteins requires the production of messenger RNAs (mRNAs), short-lived copies of the genes. The nucleotide sequences of these mRNAs are then converted by huge protein-RNA ...
... The conversion of genetic information stored in the DNA as a sequence of nucleotides into functional proteins. The synthesis of proteins requires the production of messenger RNAs (mRNAs), short-lived copies of the genes. The nucleotide sequences of these mRNAs are then converted by huge protein-RNA ...
Speciation and Gene Flow between Snails of Opposite Chirality
... common chiral morph to fixation. This therefore suggests a general mechanism as to how chiral variation arises in snails. In populations that contain both chiral morphs, two different situations are then possible. In the first, gene flow is substantial between morphs even without interchiral mating, ...
... common chiral morph to fixation. This therefore suggests a general mechanism as to how chiral variation arises in snails. In populations that contain both chiral morphs, two different situations are then possible. In the first, gene flow is substantial between morphs even without interchiral mating, ...
PDF
... congenital malformations, including polydactyly and facial clefting. Although phenotypically similar to talpid3, talpid2 has a distinct facial phenotype and an unknown cellular, molecular and genetic basis. We set out to determine the etiology of the craniofacial phenotype of this mutant. We confirm ...
... congenital malformations, including polydactyly and facial clefting. Although phenotypically similar to talpid3, talpid2 has a distinct facial phenotype and an unknown cellular, molecular and genetic basis. We set out to determine the etiology of the craniofacial phenotype of this mutant. We confirm ...
Biology 164 Laboratory Genetic Mutants of the Prodigiosin
... Figure 3 shows a pathway that is branched. There are six reaction steps each of which is catalyzed by a different enzyme. This means that there are potentially six auxotrophic strains that can be produced by mutation of the gene that produces the enzyme for that step. The principles are the same as ...
... Figure 3 shows a pathway that is branched. There are six reaction steps each of which is catalyzed by a different enzyme. This means that there are potentially six auxotrophic strains that can be produced by mutation of the gene that produces the enzyme for that step. The principles are the same as ...
DNA methylation controls histone H3 lysine 9 methylation
... whether such a correlation also exists for other genes like FWA (mapped at the long arm of chromosome 4) and SUPERMAN (SUP; mapped at the short arm of chromosome 3), which differ in their DNA methylation and expression levels between wild type and hypomethylation mutants (Jacobsen and Meyerowitz, 19 ...
... whether such a correlation also exists for other genes like FWA (mapped at the long arm of chromosome 4) and SUPERMAN (SUP; mapped at the short arm of chromosome 3), which differ in their DNA methylation and expression levels between wild type and hypomethylation mutants (Jacobsen and Meyerowitz, 19 ...
Jalasvuori_Vehicl
... populations of organisms, species, or even cells. Replicators refer to packages of genetic information that are responsible for any effective phenotype of the vehicle. Vehicle itself can be a cell, a multicellular organism, or, for example, the host organism of a parasite. “A vehicle is not a replica ...
... populations of organisms, species, or even cells. Replicators refer to packages of genetic information that are responsible for any effective phenotype of the vehicle. Vehicle itself can be a cell, a multicellular organism, or, for example, the host organism of a parasite. “A vehicle is not a replica ...
The Philosophy of Molecular and Developmental Biology
... at the core of all these definitions of ‘gene’ is the basic concept of a sequence that is transcribed to produce a gene product. Other authors have argued that Waters’s definition creates a merely verbal unity between ‘genes’ with different structures, different functions and different theoretical r ...
... at the core of all these definitions of ‘gene’ is the basic concept of a sequence that is transcribed to produce a gene product. Other authors have argued that Waters’s definition creates a merely verbal unity between ‘genes’ with different structures, different functions and different theoretical r ...
Centromere-Linkage Analysis and Consolidation
... name of the 10 nucleotide long primer, followed by the approximate size of the amplification product. Thus, the locus lOC.9550, on LG 20, is amplified by primer GI0 (Operon Technologies; Alameda, CA), andresults in a 950-bp amplification product. The formal locus name is followed by a letter in pare ...
... name of the 10 nucleotide long primer, followed by the approximate size of the amplification product. Thus, the locus lOC.9550, on LG 20, is amplified by primer GI0 (Operon Technologies; Alameda, CA), andresults in a 950-bp amplification product. The formal locus name is followed by a letter in pare ...
BioBank CDNA - Primerdesign Ltd
... experiments. The cDNA is reverse transcribed from high quality DNAse treated RNA from a variety of sources using an optimised blend of Oligo-dT and random nonamer primers. BioBank cDNA is therefore essentially free of genomic DNA and PCR inhibitors and covers the widest possible range of RNA and mRN ...
... experiments. The cDNA is reverse transcribed from high quality DNAse treated RNA from a variety of sources using an optimised blend of Oligo-dT and random nonamer primers. BioBank cDNA is therefore essentially free of genomic DNA and PCR inhibitors and covers the widest possible range of RNA and mRN ...
Synthesis and isolation of a single-strand
... We then set up primer extension reactions with KOD DNA polymerase supplemented with dGTP, dCTP, dTTP, and either LNA ATP or dATP, as well as a fluorophore-labeled forward primer. The PCRamplified LNA library was used as template. Amplification with fluorophore-labeled primers enabled us to discern b ...
... We then set up primer extension reactions with KOD DNA polymerase supplemented with dGTP, dCTP, dTTP, and either LNA ATP or dATP, as well as a fluorophore-labeled forward primer. The PCRamplified LNA library was used as template. Amplification with fluorophore-labeled primers enabled us to discern b ...
Parental Age Affects Somatic Mutation Rates in
... than the maternal one with respect to base substitutions (Kong et al., 2012) and replication slippage errors at microsatellites (Sun et al., 2012). It is also known that carriers of germline mutations in mismatch repair (MMR) genes in humans are prone to get colorectal cancer and that the risk depen ...
... than the maternal one with respect to base substitutions (Kong et al., 2012) and replication slippage errors at microsatellites (Sun et al., 2012). It is also known that carriers of germline mutations in mismatch repair (MMR) genes in humans are prone to get colorectal cancer and that the risk depen ...
Test Info Sheet
... Pontocerebellar hypoplasia (PCH) is a rare disorder affecting the ventral pons and cerebellum, two structures that share the same neuronal lineage during brain development. PCH has a fetal onset in most cases and appears to result from a combination of a developmental defect and progressive atrophy ...
... Pontocerebellar hypoplasia (PCH) is a rare disorder affecting the ventral pons and cerebellum, two structures that share the same neuronal lineage during brain development. PCH has a fetal onset in most cases and appears to result from a combination of a developmental defect and progressive atrophy ...
Full-Text PDF
... ε-ionone rings in the crucial bifurcation step of carotenoid biosynthesis. Among all carotenoid constituents, β-carotene (β,β-carotene) is found in all photosynthetic organisms, except for purple bacteria and heliobacteria, suggesting a ubiquitous distribution of lycopene β-cyclase activity in these ...
... ε-ionone rings in the crucial bifurcation step of carotenoid biosynthesis. Among all carotenoid constituents, β-carotene (β,β-carotene) is found in all photosynthetic organisms, except for purple bacteria and heliobacteria, suggesting a ubiquitous distribution of lycopene β-cyclase activity in these ...
RPG-Consent-aCGH NGS for aneuploidy_2015_Final
... Preliminary studies have demonstrated NGS to be similar in accuracy to other validated methods being used clinically to perform PGS in human preimplantation embryos. Nevertheless, NGS is a new experimental technique and as such, the exact accuracy and potential limitations of the technique have not ...
... Preliminary studies have demonstrated NGS to be similar in accuracy to other validated methods being used clinically to perform PGS in human preimplantation embryos. Nevertheless, NGS is a new experimental technique and as such, the exact accuracy and potential limitations of the technique have not ...
Trichothiodystrophy: Update on the sulfur
... defective synthesis of high-sulfur matrix proteins. Abnormalities in excision repair of ultraviolet (UV)damaged DNA are recognized in about half of the patients. Three distinct autosomal recessive syndromes are associated with nucleotide excision repair (NER) defects: the photosensitive form of TTD, ...
... defective synthesis of high-sulfur matrix proteins. Abnormalities in excision repair of ultraviolet (UV)damaged DNA are recognized in about half of the patients. Three distinct autosomal recessive syndromes are associated with nucleotide excision repair (NER) defects: the photosensitive form of TTD, ...
pdf - University of Pennsylvania
... Strikingly, this phenotype is suppressed when nos RNA bearing the TCE is expressed in the identical cells. The phenotype caused by ectopic Nos resembles one caused by ablation of neurosecretory cells that produce eclosion hormone (EH). However, we find that TCE-mediated regulation does not occur in ...
... Strikingly, this phenotype is suppressed when nos RNA bearing the TCE is expressed in the identical cells. The phenotype caused by ectopic Nos resembles one caused by ablation of neurosecretory cells that produce eclosion hormone (EH). However, we find that TCE-mediated regulation does not occur in ...
The Evolution of Genetic Architecture
... notions on the other was a great step forward. An important contribution was made by Cheverud & Routman (1995), who developed an explicit model of “physiological” epistasis defined without regard to allele frequencies and showed how this physiological epistasis differed from the Fisherian notion of s ...
... notions on the other was a great step forward. An important contribution was made by Cheverud & Routman (1995), who developed an explicit model of “physiological” epistasis defined without regard to allele frequencies and showed how this physiological epistasis differed from the Fisherian notion of s ...
Coin Child Lab – Answer Sheet
... while recessive genes are written as lowercase letters. Genotype shows the genes that have been passed along while phenotype is the actual observable trait that is the result of the genotype. If the genes for a given trait are both dominant or both recessive, we use the term homozygous or purebred. ...
... while recessive genes are written as lowercase letters. Genotype shows the genes that have been passed along while phenotype is the actual observable trait that is the result of the genotype. If the genes for a given trait are both dominant or both recessive, we use the term homozygous or purebred. ...
PDF
... hybridization. Expression was found not only in the central nervous system, as was expected from the expression pattern of the endogenous Ndn gene, but also to be comparatively widespread, suggesting that the promoter region did not contain all of the regulatory control sequences present in the endo ...
... hybridization. Expression was found not only in the central nervous system, as was expected from the expression pattern of the endogenous Ndn gene, but also to be comparatively widespread, suggesting that the promoter region did not contain all of the regulatory control sequences present in the endo ...
Chapter 4 - DORAS
... grown in LB broth and the appropriate antibiotics, was used to inoculate 5 ml aliquots of M63 minimal media (section 2.2). These M63 cultures were then incubated at 37oC overnight and used to seed the M63 bioassays. To prepare the bioassays, M63 agar (25 ml) supplemented with 100 μM of the iron chel ...
... grown in LB broth and the appropriate antibiotics, was used to inoculate 5 ml aliquots of M63 minimal media (section 2.2). These M63 cultures were then incubated at 37oC overnight and used to seed the M63 bioassays. To prepare the bioassays, M63 agar (25 ml) supplemented with 100 μM of the iron chel ...
Elsevier Editorial System(tm) for Diagnostic Microbiology and
... studies (Kim et al., 2005). These bacteria are ubiquitous in natural (freshwater, saltwater and soil) and hospital environments. E. meningoseptica is an important emerging pathogen responsible for severe healthcare infections (Jean et al., 2014). In clinical settings, Elizabethkingia species have be ...
... studies (Kim et al., 2005). These bacteria are ubiquitous in natural (freshwater, saltwater and soil) and hospital environments. E. meningoseptica is an important emerging pathogen responsible for severe healthcare infections (Jean et al., 2014). In clinical settings, Elizabethkingia species have be ...
Site-specific recombinase technology
Nearly every human gene has a counterpart in the mouse (regardless of the fact that a minor set of orthologues had to follow species specific selection routes). This made the mouse the major model for elucidating the ways in which our genetic material encodes information. In the late 1980s gene targeting in murine embryonic stem (ES-)cells enabled the transmission of mutations into the mouse germ line and emerged as a novel option to study the genetic basis of regulatory networks as they exist in the genome. Still, classical gene targeting proved to be limited in several ways as gene functions became irreversibly destroyed by the marker gene that had to be introduced for selecting recombinant ES cells. These early steps led to animals in which the mutation was present in all cells of the body from the beginning leading to complex phenotypes and/or early lethality. There was a clear need for methods to restrict these mutations to specific points in development and specific cell types. This dream became reality when groups in the USA were able to introduce bacteriophage and yeast-derived site-specific recombination (SSR-) systems into mammalian cells as well as into the mouse