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iron-regulatory protein 1
iron-regulatory protein 1

... noted X and Y are involved in the stem formation, and must be complementary (A/U, C/G) or form a stable weak interaction (U/G). For the putative bacterial acn-binding site, the two paired motifs, ACACAAUGC and CAUUUU, as defined by Tang and Guest [18], were searched. To determine the probability of ...
HL7 V2.5.1 Genetic Test Result Message
HL7 V2.5.1 Genetic Test Result Message

... Coordinator for Health IT published the Personalized Healthcare Detailed Use Case (Click here to see the use case) in response to a request and specifications from the American Health Information Community. The use case focuses on supporting secure access to electronic genetic laboratory results and ...
A Genetic Mosaic Analysis With a Repressible Cell
A Genetic Mosaic Analysis With a Repressible Cell

... system with the Leica Confocal Software and deconvoluted with Huygens Essential (Version 2.3.0) and subsequently processed with the Imaris 4.0.4 software (Bitplane). Mapping of lethal mutations: Lethal mutations induced on the left arm of the second chromosome were genetically mapped by screening fo ...
animal genetics
animal genetics

... Mendel’s experiments dealt with the relationship between an organism’s genotype and its phenotype. ...
Power Point
Power Point

... Mendel’s experiments dealt with the relationship between an organism’s genotype and its phenotype. ...
Molecular pathogenesis of liver adenomas and FNH - HAL
Molecular pathogenesis of liver adenomas and FNH - HAL

... resection of the nodule, the inflammatory syndrome disappeared, indicating that peripheral inflammatory proteins were effectively secreted by the tumor (102). We also found that inflammatory HCAs more frequently developed in patients presenting a high body mass index and excessive alcohol consumptio ...
USB® Thermo Sequenase Cycle Sequencing Kit
USB® Thermo Sequenase Cycle Sequencing Kit

... This kit and all enclosed reagents should be stored frozen at -20°C (NOT in a frostfree freezer). Keep all reagents on ice when removed from storage for use. The kit can conveniently be stored at +4°C for periods of up to 3 months with no loss of performance, but this should be avoided if it is expe ...
Correcting the Bias of WRIGHT`S Estimates of the Number of Genes
Correcting the Bias of WRIGHT`S Estimates of the Number of Genes

... then apply strong divergentselection on the character. When the initial frequencies of alleles are 0.5, which is the case in a cross of two inbred lines, selection can be very effective in fixing the alleles in the appropriate selection lines (ZHC).] However, even if assumption [ 11 holds, violation ...
Cell division: The renaissance of the centriole
Cell division: The renaissance of the centriole

... but when centrioles are surgically removed from somatic cells these structures cannot regenerate [6]. Thus, the role of pre-existing centrioles in centriole assembly remains controversial. To some extent, we owe this controversy to a lack of molecular information about centriole duplication. Recentl ...
DNA
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... • Human genome is around 3 billions base pair long • Almost every cell in human body contains same set of genes • But not all genes are used or expressed by those cells ...
rapid evolutionary escape by large populations from local fitness
rapid evolutionary escape by large populations from local fitness

... Abstract. Fitness interactions between loci in the genome, or epistasis, can result in mutations that are individually deleterious but jointly beneficial. Such epistasis gives rise to multiple peaks on the genotypic fitness landscape. The problem of evolutionary escape from such local peaks has been ...
Youngson and Whitelaw, 2008
Youngson and Whitelaw, 2008

... states, most gene regulatory, i.e., epigenetic, information is not transferred between generations. Several mechanisms have evolved to erase the marks, including germline and somatic reprogramming of DNA methylation and chromatin proteins. However, we know that at some loci the epigenetic marks are ...
Isolation, Characterization and Complementation
Isolation, Characterization and Complementation

... In summary, only two of the mutant classes described by Abou-JaoudC et al. (1978a) were deficient in their ability to reduce nitrite and only the nirD strain was defective in NADHdependent nitrite reductase activity. The phenotype of LCB82 was identical to that of strain JCB203, but although both of ...
Muscle pattern diversification in Drosophila: the story of
Muscle pattern diversification in Drosophila: the story of

... positions. This specific distribution of myoblasts could involve an active migration of these cells to the presumptive notal region as the disc grows and expands during larval life, or there could be specific signals in the prospective wingblade region that prevents these cells from distributing the ...
Selection in backcross programmes
Selection in backcross programmes

... and yield) between maize elite lines with markerassisted backcrossing. They showed that the use of markers to improve background selection is efficient, even with few markers, especially on non-carrier chromosomes. Foreground selection on markers to control the three target regions without the help ...
Inclusive Fitness and the Sociobiology of the Genome Herbert Gintis
Inclusive Fitness and the Sociobiology of the Genome Herbert Gintis

... Hamilton’s Disharmony Principle: A predatory gene that is harmful may satisfy Hamilton’s rule despite being antisocial. where we say an allele is antisocial if it is evolutionarily successful at the expense of mean population fitness. Moreover, in our basic equation (3) for Hamilton’s rule, p < q is ...
Mapping the genes that made maize
Mapping the genes that made maize

... chromosomal locations of these genes. As will be discussed, recent developments in molecular biology and quantitative genetics have provided some new insights into these old issues. ...
Musil et al - Merit Research Journals
Musil et al - Merit Research Journals

... Bilateral renal agenesis (BRA) is incompatible with life. It occurs in about one in 30,000 births (Schedl, 2007). Unilateral renal agenesis (URA) is more common than BRA. The most quoted URA frequency is one in 4,0005,000 births (Schedl, 2007; Reiterová and Merta, 2008). The remarkable fact is that ...
Pleiotropic effects of the mouse lethal yellow (Ay) mutation
Pleiotropic effects of the mouse lethal yellow (Ay) mutation

... 25 blastocysts as previously described (Andria et al., 1992) was reverse transcribed using an oligonucleotide primer complementary to sequences in the 3′ end of the Merc open reading frame (see Fig. 4A), 5′-ACCATCATCTCGAATTTG-3′, and then PCR-amplified using the oligonucleotide primers 5′-CAGGGCCGCC ...
The population genetics of mutations: good, bad and indifferent
The population genetics of mutations: good, bad and indifferent

... associated with or indirectly influenced by his work. In this collection of papers, various aspects are considered in detail, and in this introduction, we aim to ...
AP Biology Notes Outline Chapter 27 Bacteria and Archaea Concept
AP Biology Notes Outline Chapter 27 Bacteria and Archaea Concept

... Concept 27.5: Prokaryotes and the Biosphere Prokaryotes play crucial roles in the biosphere:  Many prokaryotes are decomposers, breaking down dead organic matter.  Many prokaryotes are symbiotic, forming crucial relationships with other species.  Some prokaryotes are pathogenic and cause illness ...
Genome-wide scan of bipolar disorder in 65 pedigrees: supportive evidence for linkage at 8q24, 18q22, 4q32, 2p12, and 13q12.
Genome-wide scan of bipolar disorder in 65 pedigrees: supportive evidence for linkage at 8q24, 18q22, 4q32, 2p12, and 13q12.

... genome in several ways. First, we used an approach described previously19 to divide the 65 pedigrees according to the parental lineage of the disorder, then we carried out linkage analyses with the sib_ibd routine of ASPEX (v2.2) in the two groups separately. Among the 65 pedigrees, there were 23 pa ...
What is a biofilm?
What is a biofilm?

... • Patients eventually succumb to the lung damage inflicted by the bacterial infection, with a life expectancy of ...
Head induction in the chick - Max-Planck
Head induction in the chick - Max-Planck

... node/mesendoderm, the definitive cause of these defects remained unclear. Several other experiments have addressed the role of the visceral endoderm more directly. Chimeric mouse embryos composed predominantly of wild-type cells, but entirely of Nodal−/− or Otx2−/− cells in the visceral endoderm, di ...
Specification of floral organs in Arabidopsis
Specification of floral organs in Arabidopsis

... in the revised model] are predominantly involved in specifying floral meristems, not floral organs, and mediate floral patterning by ensuring the correct expression domains of the B and C function regulators. According to this model, sepals are the default state for floral organs (see above). Hence, ...
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Site-specific recombinase technology



Nearly every human gene has a counterpart in the mouse (regardless of the fact that a minor set of orthologues had to follow species specific selection routes). This made the mouse the major model for elucidating the ways in which our genetic material encodes information. In the late 1980s gene targeting in murine embryonic stem (ES-)cells enabled the transmission of mutations into the mouse germ line and emerged as a novel option to study the genetic basis of regulatory networks as they exist in the genome. Still, classical gene targeting proved to be limited in several ways as gene functions became irreversibly destroyed by the marker gene that had to be introduced for selecting recombinant ES cells. These early steps led to animals in which the mutation was present in all cells of the body from the beginning leading to complex phenotypes and/or early lethality. There was a clear need for methods to restrict these mutations to specific points in development and specific cell types. This dream became reality when groups in the USA were able to introduce bacteriophage and yeast-derived site-specific recombination (SSR-) systems into mammalian cells as well as into the mouse
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