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Pedigree Questions from VCAA Exams with answers
Pedigree Questions from VCAA Exams with answers

... 62% of people got this correct Question 2 Some gene loci have several alternative alleles. With these gene loci, the DNA profi le of an individual may be given in terms of the relative sizes of the alleles. For example, gene locus THO has three alleles that are called THO 7, THO 9 and THO 10. In a m ...
Myb genes enhance tobacco trichome production
Myb genes enhance tobacco trichome production

... Both GL1 and TTG 1 are necessary for normal Arabidopsis trichome initiation. The GL1 gene was cloned by T-DNA tagging (Marks and Feldmann, 1989). It encodes a putative transcription factor with myb-type DNA binding and Cterminal acidic activation domains (Oppenheimer et al., 1991). By itself GL1 is ...
Genetic analysis of metabolites in apple fruits indicates an mQTL
Genetic analysis of metabolites in apple fruits indicates an mQTL

... Mapping of the metabolites that segregated as a monogenetic trait The metabolites procyanidin dimer I, procyanidin dimer II, procyanidin trimer I, procyanidin trimer II, (+)-catechin, and (–)-epicatechin had only one mQTL, segregating in a clear 3:1 ratio. This single locus explained a major part of ...
No Slide Title
No Slide Title

... • Phosphorylation changes the properties of a protein, by changing its conformation • Changes in conformation can activate or inhibit a biochemical activity, or create a binding site for other proteins • Phosphorylation is rapid and requires no protein synthesis or degradation to change the biochemi ...
Slide 1
Slide 1

... The results were astounding. One of the markers, located right in the middle of a region previously linked to several behavioral traits, showed a huge correlation to chopstick use, enough to account for nearly half of the observed variance. When the experiment was repeated with students from a diffe ...
Genes for control of form and
Genes for control of form and

... synonyms include anatomy, appearance, architecture, design, model, and structure. Both stature and form show plasticity, which is itself widely known to vary among genotypes and species. However, consideration of genotype × environment dimensions of the control of plant stature and form are beyond t ...
T cell antigen receptor diversity Generation of
T cell antigen receptor diversity Generation of

... • Phosphorylation changes the properties of a protein, by changing its conformation • Changes in conformation can activate or inhibit a biochemical activity, or create a binding site for other proteins • Phosphorylation is rapid and requires no protein synthesis or degradation to change the biochemi ...
Direct Deletion Analysis in Two Duchenne Muscular Dystrophy
Direct Deletion Analysis in Two Duchenne Muscular Dystrophy

... We describe here the molecular analysis of two symptomatic DMD/BMD females by genetic segregation of polymorphic markers. The detection of a heterozygous deletion by this method was successful in both of the cases that were studied. There was evidence of parental origin of the deleted allele in both ...
E.coli
E.coli

... Can we use this signal to deduce some more biological information ? We determined the most important metabolic networks in a (translationally biased) organism Can we determine genes belonging to minimal gene sets ? ...
Introduction to RNA sequencing
Introduction to RNA sequencing

... – Genome may be constant but an experimental condition has a pronounced effect on gene expression • e.g. Drug treated vs. untreated cell line • e.g. Wild type versus knock out mice ...
KARNATAKA COMMON ENTRANCE TEST – MAY, 2016 BIOLOGY
KARNATAKA COMMON ENTRANCE TEST – MAY, 2016 BIOLOGY

... Disclaimer: These solutions are provided by the academic team of BASE. These solutions may be different from those to be provided by the Karnataka Examinations Authority (KEA) due to difference in assumptions taken in support of the solutions. In such cases solution provided by KEA will be deemed as ...
Reduced X-linked nucleotide polymorphism in Drosophila simulans
Reduced X-linked nucleotide polymorphism in Drosophila simulans

... Edited by James F. Crow, University of Wisconsin, Madison, WI, and approved February 25, 2000 (received for review November 24, 1999) ...
Hb_lab_Activities_Guide - AIM-UP!
Hb_lab_Activities_Guide - AIM-UP!

... investigate the genetic basis for these functional types and hypothesize how different amino acid substitutions impact hemoglobin function. Use the Activity Sheet to record your answers. Task I-I: Finding locality and sequence data on HBA in deer mice (P. maniculatus) using databases: Arctos (http:/ ...
Analysis of Gene expression data using MATLAB Software
Analysis of Gene expression data using MATLAB Software

... simultaneously. The underlying concept, which is often referred to as biclustering, allows to identify sets of genes sharing compatible expression patterns across subsets of samples, and its usefulness has been demonstrated for different organisms and datasets. Several biclustering methods have been ...
cached copy
cached copy

... DNA molecule with connectivity greater than two. The cube self-assembles from pieces of DNA designed to adhere to one another, but the ends of each piece do not join up. Ligases can connect these free ends, resulting in six closed loops, one for each face of the cube. Because of the helical nature o ...
Mutation in xyloglucan 6-xylosytransferase results in abnormal root
Mutation in xyloglucan 6-xylosytransferase results in abnormal root

... simple sequence repeat (SSR) markers RM232 and RM3280 using 1800 F2 mutant plants. SSR markers were obtained from NCBI database (http://www.ncbi.nlm.nih.gov/unists). The mutation was further mapped to a 36-kb region between STS274-04 and STS27404-06 using nine newly developed SSR markers. Based on t ...
Identification of a mutation in LARS as a novel cause of infantile
Identification of a mutation in LARS as a novel cause of infantile

... models amino acid substitutions using both structure and sequence information, comparing a property of the wild-type (ancestral, normal) allele and the corresponding property of the mutant (derived, diseasecausing) allele. The functional importance of an allele replacement is predicted from its indi ...
Physiological characterization of natural transformation in
Physiological characterization of natural transformation in

... all the HindIII sites that are present in these plasmids. ...
REVIEW Why Do Bacterial Plasmids Carry Some Genes and Not
REVIEW Why Do Bacterial Plasmids Carry Some Genes and Not

... bacterial geneshave been in both plasmids and increase the reproduction of units at one level chromosomes repeatedly during their evolu- while decreasing reproduction at others. Hitionary lives. This must be especially true for erarchical living systems show “emergent” genesassociatedwith insertion ...
variation in the strength and softness of selection on
variation in the strength and softness of selection on

... The framework for the likelihood model is based on the following logic. Let ei,j be the number of eggs of genotype i placed in deme j and ni,j be the number of surviving adults of this genotype from this deme. We calculated the likelihood of observing n+,j wild-type adults and nx,j mutant adults in ...
Identification and mapping of RAPD and RFLP markers linked to a
Identification and mapping of RAPD and RFLP markers linked to a

... Merdinoglu et al. (1995) was referred to in order to identify the linkage group that contained the markers linked to the restorer locus. Then the maps by Barzen et al. (1995) and Schondelmaier et al. (1995), including RFLP, RAPD and morphological markers, were used to produce a joint map of the regi ...
VI. Levels of Selection
VI. Levels of Selection

... • spermatid degeneration of Y-bearing sperm in male carriers of X(d). • balanced by Y-linked and autosomal factors that decrease the intensity of meiotic drive. • Even a Y-linked polymorphism for resistance to drive which reduces the intensity and reverses the direction of meiotic drive. ...
Fact Sheet 52|HAEMOPHILIA WHAT IS HAEMOPHILIA
Fact Sheet 52|HAEMOPHILIA WHAT IS HAEMOPHILIA

... Our body is made up of millions of cells, and in each cell there are instructions, called genes, that make all the necessary structural components and chemicals for the body to function. These genes are packaged onto little long strands known as chromosomes. We all have 46 chromosomes arranged into ...
Methods for detecting positive selection and examples among fungi
Methods for detecting positive selection and examples among fungi

... rate. The Bayesian method can be employed to infer which sites in the alignment are under positive selection. This method is used to compute the posterior probability that each site belongs to a particular v class. A site with a posterior probability (0.95) of belonging to the class of sites with v ...
XLibraryDisplay User Manual Ryan Stafford
XLibraryDisplay User Manual Ryan Stafford

... Several features are available by right-clicking on the alignment including marking and unmarking the library positions, showing a local DNA amino acid alignment, editing the DNA sequence, removing the sequence from the alignment (which also transfers the DNA from the GoodDNA to the BadDNA sheet), a ...
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Site-specific recombinase technology



Nearly every human gene has a counterpart in the mouse (regardless of the fact that a minor set of orthologues had to follow species specific selection routes). This made the mouse the major model for elucidating the ways in which our genetic material encodes information. In the late 1980s gene targeting in murine embryonic stem (ES-)cells enabled the transmission of mutations into the mouse germ line and emerged as a novel option to study the genetic basis of regulatory networks as they exist in the genome. Still, classical gene targeting proved to be limited in several ways as gene functions became irreversibly destroyed by the marker gene that had to be introduced for selecting recombinant ES cells. These early steps led to animals in which the mutation was present in all cells of the body from the beginning leading to complex phenotypes and/or early lethality. There was a clear need for methods to restrict these mutations to specific points in development and specific cell types. This dream became reality when groups in the USA were able to introduce bacteriophage and yeast-derived site-specific recombination (SSR-) systems into mammalian cells as well as into the mouse
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