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Synapsis-Mediated Fusion of Free DNA Ends Forms Inverted Dimer Plasmids in Yeast.
Synapsis-Mediated Fusion of Free DNA Ends Forms Inverted Dimer Plasmids in Yeast.

... 1985). A third pathway for processing free DNA ends in yeast is observed when, in the absence of chromosomal homology, a linearized plasmid is introduced along with an excess of highly fragmented nonhomologous carrier DNA (KUNES, BOTSTEIN and Fox 1985). The presence of carrier DNA results in a new p ...
Photosynthesis
Photosynthesis

... Rate at which allelic patterns are disrupted by crossing over:  Indicates distance between loci  Can be used to develop linkage map or genetic map of chromosome ...
Sex Linked Inheritance, Chromosome Mapping
Sex Linked Inheritance, Chromosome Mapping

... Linkage maps estimate distances between genes. • The closer together two genes are, the more likely they will be inherited together. • Cross-over frequencies are related to distances between genes. • Linkage maps show the relative locations of genes. ...
Belote, J. m., F. M. Hoffmann, M. McKeown, R. Chorsky, and B. S. Baker. (1990). Cytogenetic analysis of chromosome region 73AD of Drosophila melanlgaster. Genetics 125: 783-793.
Belote, J. m., F. M. Hoffmann, M. McKeown, R. Chorsky, and B. S. Baker. (1990). Cytogenetic analysis of chromosome region 73AD of Drosophila melanlgaster. Genetics 125: 783-793.

... visible mutants were recovered. The 45 X-ray-induced mutants (21 st alleles, 21 non-st lethals, and 3 non-st visibles) were analyzed cytologically to see if they were associated with gross chromosomal rearrangementsin the 73AD region. Of the 21 st mutants, 13 were associated with chromosome rearrang ...
Chapter 13
Chapter 13

... 1. Natural selection. If selection favors individuals with particular combinations of alleles, then it produces linkage disequilibrium. If two or more gene combinations are much fitter than recombinant genotypes linkage disequilibrium will be favored. 2. Non-random mating. 3. When a new mutation ari ...
Analyzing microRNA Data and Integrating microRNA with Gene
Analyzing microRNA Data and Integrating microRNA with Gene

... interested in identifying which microRNAs might regulate the significant genes in that experiment. Using a database like TargetScan, you can create a list of microRNAs that are statistically predicted to regulate those genes which could be explored in further experiments by using a lower-volume tech ...
Lampbrush Chromosomes of the Chicken
Lampbrush Chromosomes of the Chicken

... of meiotic prophase and are characterized by extensive transcription on the loops. An excellent review of LBC investigations and techniques has just been published by Callan (1986). This reference should be consulted for a more detailed discussion. Despite a century of study, we still know relativel ...
Mycobacterium tuberculosis: Potassium uptake systems of
Mycobacterium tuberculosis: Potassium uptake systems of

Rather than test an unmapped gene successively for linkage to... groups, it is advantageous to test all linkage groups in... alcoy David Perkins
Rather than test an unmapped gene successively for linkage to... groups, it is advantageous to test all linkage groups in... alcoy David Perkins

... temperature sensitive-1, yellow-1, and conidial separation-2 (which marks linkage group VII). All four are readily scored by eye. The strains grow on minimal medium. No transfer to test-media is necessary. Crosses heterozygous for alcoy .have proved useful in cytological studies of the synaptonemal ...
vilniaus universitetas
vilniaus universitetas

... required for its own degradation. Nevertheless, Achromobacter sp. JS18, due to its ability to transform a wide range of N-heterocyclic compounds, is very attractive for the biosynthesis of hydroxylated aromatic carboxylic acids. A new bacterial strain Sinorhizobium sp. L1, capable to utilize 3-hydro ...
University of Groningen Live-Cell Imaging Tool Optimization To
University of Groningen Live-Cell Imaging Tool Optimization To

... division, biofilm formation (7), sporulation (8), and spore germination (9). In this way, B. cereus is becoming an increasingly studied model organism for Gram-positive pathogenic bacteria next to its well-known but fairly distant and nonpathogenic relative Bacillus subtilis. In contrast to such stu ...
Coding Regions of MSX1 do not Contribute to Non
Coding Regions of MSX1 do not Contribute to Non

... In our study, we detected no sequence variations in the examined regions of MSX1, two exons including intronicexonic boundaries in Turkish NS-CL/P patients. There are some reports that researchers found no MSX1 mutations in patients with clefts [8, 9]. According to the previous studies, mutations in ...
The Novel Gene HOMOLOGOUS PAIRING
The Novel Gene HOMOLOGOUS PAIRING

... required for homologous chromosome pairing and cytokinesis in male and female meiocytes of rice (Oryza sativa). The pair1 mutation, tagged by the endogenous retrotransposon Tos17, exhibited meiosis-specific defects and resulted in complete sterility in male and female gametes. The PAIR1 gene encodes ...
Development of Co-Dominant Amplified Polymorphic Sequence
Development of Co-Dominant Amplified Polymorphic Sequence

... a chromosome marker set derived from the mapping population developed at Cornell in the late 1980s and early 1990s (17). However, all markers used in this report are derived from the highdensity RFLP map developed by the Nipponbare and Kasalath cross (5). Subsequent to the creation of the Nipponbare ...
Genetic determinants of sense organ identity in
Genetic determinants of sense organ identity in

... poxn cannot promote es development in the absence of cut In the absence of cut, no es organ is formed (Bodmer et al., 1987). Yet we observed in AS-C− embryos that ch precursors (which do not express cut) can form es organs if they are forced to express poxn. One way to explain this result is that po ...
第六届植物分子生物学暑期研讨班专家资料介绍
第六届植物分子生物学暑期研讨班专家资料介绍

characters found in indica xjaponica
characters found in indica xjaponica

... Two subspecies of common rice, indica and japonica, are distinguishable by differences in alleles at a number of loci controlling biochemical and morphological traits. The nature of associations between twelve alleles and phenotypes was studied in both a sample of varieties and hybrid populations de ...
Pedigree Charts
Pedigree Charts

... thousand genes but few, if any, of these have anything to do directly with sex determination. The X chromosome likely contains genes that provide instructions for making proteins. These proteins perform a variety of different roles in the body. ...
A Sickle-cell Crisis
A Sickle-cell Crisis

... Sickle-cell anaemia is inherited in the autosomal recessive pattern (see Fig.1.1). If one parent has sickle-cell anaemia (both sickle-cell causing genes) and one parent has the sickle-cell trait (one of the sickle cell causing genes) there is a 50% chance that their decadences will have either sickl ...
Homologous recombination in budding yeast expressing the human
Homologous recombination in budding yeast expressing the human

... expression was controlled by the ADH1 promoter and terminator sequences. Western blots of whole cell extracts from strains containing the adh1::HsRAD52-FLAG allele displayed a 49 kDa peptide consistent with that expected for HsRAD52-FLAG while extracts from strains containing the RAD52-FLAG allele y ...
MOLECULAR BIOLOGY AND BIOTECHNOLOGY
MOLECULAR BIOLOGY AND BIOTECHNOLOGY

... MBB 502/ FUNDAMENTALS OF MOLECULAR BIOLOGY 3+0 SEM - I BIOCHEM 504/ (To be taught jointly by Molecular Biology & Biotechnology, Biochemistry and BIF508 Bioinformatics) ObjectiveT o familiarize the students with the basic cellular processes at molecular level. Theory UNIT-I: Historical developments o ...
c-Myc co-ordinates mRNA cap methylation and ribosomal RNA
c-Myc co-ordinates mRNA cap methylation and ribosomal RNA

... Figure 1. Expression of the mRNA cap methyltransferase complex, RNMT–RAM, is required for 45S rRNA production. HeLa cells were transfected into two independent RAM siRNAs, an RNMT siRNA and a non-targeting control siRNA, for 48 h. (A) Expression of RNMT, RAM and GAPDH was analysed by western blot. ( ...
Chapter 15
Chapter 15

... Recombination of Unlinked Genes: Independent Assortment of Chromosomes • Mendel observed that combinations of traits in some offspring differ from either parent • Offspring with a phenotype matching one of the parental phenotypes are called parental types • Offspring with nonparental phenotypes (ne ...
REVIEWS - The SHINE Library
REVIEWS - The SHINE Library

... Indeed, the roof plate acts as an important organizing center for the secretion of these morphogens that are crucial for establishing the dorsal-lateral identity along the central nervous system (CNS) (31, 58, 59). Although malformation of the SCO during gestation leads to ventricular dilation and a ...
against Drought - Max-Planck
against Drought - Max-Planck

... There are several phases in the development of barley flowers. First, vegetative growth tissue (meristem, A) forms the flower meristem, B. From this is formed the spike with the meristem primordia (C), which then develops into fertile florets (D) as the stem elongates. The duration of each of these ...
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Site-specific recombinase technology



Nearly every human gene has a counterpart in the mouse (regardless of the fact that a minor set of orthologues had to follow species specific selection routes). This made the mouse the major model for elucidating the ways in which our genetic material encodes information. In the late 1980s gene targeting in murine embryonic stem (ES-)cells enabled the transmission of mutations into the mouse germ line and emerged as a novel option to study the genetic basis of regulatory networks as they exist in the genome. Still, classical gene targeting proved to be limited in several ways as gene functions became irreversibly destroyed by the marker gene that had to be introduced for selecting recombinant ES cells. These early steps led to animals in which the mutation was present in all cells of the body from the beginning leading to complex phenotypes and/or early lethality. There was a clear need for methods to restrict these mutations to specific points in development and specific cell types. This dream became reality when groups in the USA were able to introduce bacteriophage and yeast-derived site-specific recombination (SSR-) systems into mammalian cells as well as into the mouse
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