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An Approximate Approach to DNA Denaturation
An Approximate Approach to DNA Denaturation

... Let us briefly review the development of experimental work t h a t has been done in recent years. The experimental background comes mainly from R a m a n and IR spectroscopic results t h a t are an alternative approach (Urabe and Tominaga 1981), more closely connected to theoretical lattice dynamics ...
Journal of Experimental Botany
Journal of Experimental Botany

... To assess whether LHA1, LHA2 and LHA4 gene expression could be involved in the elicitation of the general plant defence response to a pathogen attack, expression of these genes was also analysed in roots of tomato plants infected with the root pathogen P. parasitica. Infection of tomato roots by P. ...
Identification and Analysis of Arabidopsis Expressed Sequence
Identification and Analysis of Arabidopsis Expressed Sequence

... 2001). Analyses of the properties and functions of ncRNAs have indicated that they can act as gene regulators, as part of biotic and abiotic stress signals, or as part of RNA-protein complexes with various enzymatic and structural activities. A number of ncRNAs are processed in an mRNA-like manner; ...
Single-Gene Inheritance Single-Gene Inheritance
Single-Gene Inheritance Single-Gene Inheritance

... crosses. The central components in this type of analysis are mutants, individual organisms having some altered form of a normal property. The normal form of any property of an organism is called the wild type, that which is found “in the wild,” or in nature. The genetic modus operandi is to mate an ...
Relationship between chromosome fragility, aneuploidy and
Relationship between chromosome fragility, aneuploidy and

Mutational effects depend on ploidy level: all else is not equal
Mutational effects depend on ploidy level: all else is not equal

... A factor that has been left out of this (and similar) discussions is whether all else is equal between individuals of different ploidy. Although direct empirical tests are relatively sparse, the existing data suggest that ploidy background may well affect the properties of mutations. The effect size ...
Gene Section USF1 (upstream transcription factor 1) Atlas of Genetics and Cytogenetics
Gene Section USF1 (upstream transcription factor 1) Atlas of Genetics and Cytogenetics

Inherited Representations are Read in
Inherited Representations are Read in

... acquired the adaptive function of transmitting information down the generations (see also Godfrey-Smith [2010]; Shea [2011a]). That function can form the basis of a teleosemantic theory according to which DNA represents whole organism phenotypes.1 Sceptics about the importance of genetic representat ...
et al - International Journal of Systematic and Evolutionary
et al - International Journal of Systematic and Evolutionary

... these proteins was previously available from only a limited number of actinobacteria, whose genomes have been sequenced. One possible signature for actinobacteria, consisting of a large insert in the 23S rRNA, has previously been described (Roller et al., 1992). However, the validity and specificity ...
Do gametes woo? Evidence for non-random unions at
Do gametes woo? Evidence for non-random unions at

... facilitate comparison with observed genotype distributions (Tables 1,2), calculated TR, the ratio of heterozygotes to WT. In intercrosses without TRD, this ratio should be 2 (= 2 heterozygotes to 1 WT). Results for one sex- and both sex-TRD are remarkably similar with an excess of heterozygotes aris ...
Applied Animal Breeding and Gene
Applied Animal Breeding and Gene

... The examination of most animal species reveals the existence of phenotype difference between individuals for example in cattle there are obvious difference in coat colours and the presence and absence of horns. If cattle are weighed or if milk production is recorded there will be difference in perfo ...
Heritable Disorders of Connective Tissue: Research & Repository
Heritable Disorders of Connective Tissue: Research & Repository

... from families, friends to UW (Byers)) 2009 Freudman Fund - family foundation to support research in EDS (basic science research and clinical research) and related connective tissue disorders ...
NAME_________KEY____________________________ Page 2
NAME_________KEY____________________________ Page 2

... c) (2 points) Suppose each of the nucleotide positions were analyzed one-by-one in humans for associations with elevated levels of trait X. Which nucleotide positions would show such an association? Because all humans have the mutation at site 1, it cannot be a cause of variation. The SNP at site 7 ...
Slide 1
Slide 1

... • To test for under-enrichment of “black”, test for over-enrichment of “red”. • The EASE score used by DAVID subtracts one from the observed overlap between gene list and gene set to ensure >1 from the list is in the gene set. • Need to choose “background population” appropriately, e.g., if only por ...
Sterile, 24-well tissue culture plates are filled with melted minimal ... 1.0 ml per well using a repeating syringe. After the...
Sterile, 24-well tissue culture plates are filled with melted minimal ... 1.0 ml per well using a repeating syringe. After the...

... can be used immediately or stored for several weeks in plastic bags at 5°C. complementation tests, each well of a plate is inoculated with a drop of spore suspension from an auxotrophic mutant (usually nitM) of a VCG standard strain. A sterile Pasteur pipette is used for inoculations. A drop of spor ...
A Single Gene Causes Both Male Sterility and
A Single Gene Causes Both Male Sterility and

... genomic forces that drive the evolution of postzygotic isolation. Here, we show that the same gene, Overdrive, causes both male sterility and segregation distortion in F1 hybrids between the Bogota and U.S. subspecies of Drosophila pseudoobscura. This segregation distorter gene is essential for hybr ...
Two distinct teleost hepatocyte nuclear factor 1 genes, hnf1a/tcf1
Two distinct teleost hepatocyte nuclear factor 1 genes, hnf1a/tcf1

... defects in human hnf1b gene cause not only maturityonset diabetes (MODY5) (Horikawa et al., 1997), but also renal defects and genital malformation (Ryffel, 2001). Apart from its functional roles in adult organs, hnf1b is required for embryonic development including visceral endoderm, ectoderm differ ...
Antigenic diversity of Theileria major piroplasm surface protein gene
Antigenic diversity of Theileria major piroplasm surface protein gene

... isolates were type I, and the cattle exhibited severe symptoms [5]. In our study, type I was rare (6 of 35); most of the isolates were types C (20 of 35) and B (17 of 35), and all cattle were normal clinically and on hematological examination. In this study, we used PCR-RLFP to subclassify type B, a ...
Mutant Fruit Flies: Exploratorium Exhibit. Mutations in
Mutant Fruit Flies: Exploratorium Exhibit. Mutations in

... The fruit flies in this exhibit show just a few of the mutations that occur in natural fruit fly populations. The genetic instructions to build a fruit fly-or any other organism-are imprinted in its DNA, a long, threadlike molecule packaged in bundles called chromosomes. Like a phone book made up of ...
Parasites, desiderata lists and the paradox of the organism
Parasites, desiderata lists and the paradox of the organism

... paradox at all! They have wrongly taken the organism for granted as the unit about which questions of adaptation should be asked. It is possible to do the mathematics of natural selection completely forgetting that, as a matter of fact, the genes are not swishing about in a liquid pool or river but ...
MOLECULAR CYTOGENETIC ANALYSES IN WHEAT AND
MOLECULAR CYTOGENETIC ANALYSES IN WHEAT AND

... make detailed studies on the degree of relationship between the various plant species and varieties and on their genome structures. In recent decades many results have been achieved in this field. Basic breeding material with more favourable agronomic properties have been developed carrying genes or ...
Building Better Beef The Building Blocks Behind Wagyu By: Tracy
Building Better Beef The Building Blocks Behind Wagyu By: Tracy

... dominate AA can be an important factor, it is part of a broader set of criteria used to produce high quality Wagyu meat. Bell stated they rely on multiple genetic tests as part of selection criterion for their herd. “We have only had a minimal number of American Wagyu producers send in hair and bloo ...
Myb genes enhance tobacco trichome production
Myb genes enhance tobacco trichome production

... Both GL1 and TTG 1 are necessary for normal Arabidopsis trichome initiation. The GL1 gene was cloned by T-DNA tagging (Marks and Feldmann, 1989). It encodes a putative transcription factor with myb-type DNA binding and Cterminal acidic activation domains (Oppenheimer et al., 1991). By itself GL1 is ...
Familial Mediterranean fever (FMF)
Familial Mediterranean fever (FMF)

... the patients undergo useless appendectomy. The familial notion is becoming less-and-less common because of the autosomal recessive inheritance and the tendency towards smaller families. The following diseases can be excluded for diverse reasons: • recurrent fever with hyper IgD does not affect the s ...
Lack of biological significance in the `linguistic features` of
Lack of biological significance in the `linguistic features` of

... say, Di = 200 bp), using 6-tuples as ‘words’ produced essentially indistinguishable graphs. We obtained essentially the same ‘negative’ result for many different natural DNAs, among others: (i) the human HSRETBLAS (cf. above), and (ii) the E.coli sequence ECUW89 (cf. above); see Figure 4b. These res ...
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Site-specific recombinase technology



Nearly every human gene has a counterpart in the mouse (regardless of the fact that a minor set of orthologues had to follow species specific selection routes). This made the mouse the major model for elucidating the ways in which our genetic material encodes information. In the late 1980s gene targeting in murine embryonic stem (ES-)cells enabled the transmission of mutations into the mouse germ line and emerged as a novel option to study the genetic basis of regulatory networks as they exist in the genome. Still, classical gene targeting proved to be limited in several ways as gene functions became irreversibly destroyed by the marker gene that had to be introduced for selecting recombinant ES cells. These early steps led to animals in which the mutation was present in all cells of the body from the beginning leading to complex phenotypes and/or early lethality. There was a clear need for methods to restrict these mutations to specific points in development and specific cell types. This dream became reality when groups in the USA were able to introduce bacteriophage and yeast-derived site-specific recombination (SSR-) systems into mammalian cells as well as into the mouse
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