Increased Yield of PCR Products by Addition of T4 Gene 32 Protein
... require large amounts of mRNA. Several procedures of cDNA amplification and enrichment are available, but one of the most efficient systems is the SMART PCR cDNA synthesis kit (Clontech Laboratories, Palo Alto, CA, USA). Interest in these systems stems from the fact that they require very small amo ...
... require large amounts of mRNA. Several procedures of cDNA amplification and enrichment are available, but one of the most efficient systems is the SMART PCR cDNA synthesis kit (Clontech Laboratories, Palo Alto, CA, USA). Interest in these systems stems from the fact that they require very small amo ...
Why genes are regulated?
... Gene activator: AR1 (activating region 1) region within the C-terminal domain, which interacts with the C-terminal domain of the RNAP alpha subunit (aCTD); AR2 (activating region 2) region within the N-terminal domain, which interacts with the N-terminal domain of RNAP alpha subunit (aNTD); AR3 (act ...
... Gene activator: AR1 (activating region 1) region within the C-terminal domain, which interacts with the C-terminal domain of the RNAP alpha subunit (aCTD); AR2 (activating region 2) region within the N-terminal domain, which interacts with the N-terminal domain of RNAP alpha subunit (aNTD); AR3 (act ...
Supporting Information S1: 1. Establishment of hSMP30 transcription
... To determine the transcription initiation site of SMP30, mRNA prepared from MCF-7 cell line was reverse transcribed by 32p labelled specific primer and then resolved in a sequencing gel along with the relevant genome sequence. A primer extension product was found 203bp from exon 1 of SMP30 mRNA (Fig ...
... To determine the transcription initiation site of SMP30, mRNA prepared from MCF-7 cell line was reverse transcribed by 32p labelled specific primer and then resolved in a sequencing gel along with the relevant genome sequence. A primer extension product was found 203bp from exon 1 of SMP30 mRNA (Fig ...
Cellular Pathophysiology of Insulin Resistance
... proteins (eg, IRS) on the amino acid tyrosine. • Phosphorylation creates recognition sites for IRS and other proteins to serve as scaffolds, recruiting other molecules into signaling complexes. • Phosphorylation on other sites can oppose complex formation. ...
... proteins (eg, IRS) on the amino acid tyrosine. • Phosphorylation creates recognition sites for IRS and other proteins to serve as scaffolds, recruiting other molecules into signaling complexes. • Phosphorylation on other sites can oppose complex formation. ...
Power Point
... Promoters of the GAL7, GAL10 and GAL1 genes contain multiple binding sites for the Gal4p transcriptional activator ...
... Promoters of the GAL7, GAL10 and GAL1 genes contain multiple binding sites for the Gal4p transcriptional activator ...
NisimNaim-AdiPotok
... The cells are illuminated with light of a certain wavelength and emit light of a different wavelength. This technique is used to acquire 3D images of a specimen. Each image is composed of several 2D layers. ...
... The cells are illuminated with light of a certain wavelength and emit light of a different wavelength. This technique is used to acquire 3D images of a specimen. Each image is composed of several 2D layers. ...
Full text PDF
... showed that the transactivation domain of CRTC2 associates with a class of histone acetyltransferase KAT2B, leading to the increased acetylation of histone 3 lysine 9 (H3K9) of gluconeogenic promoters, resulting in the increased association of CREB-CRTC2 and transcriptional activation of target gene ...
... showed that the transactivation domain of CRTC2 associates with a class of histone acetyltransferase KAT2B, leading to the increased acetylation of histone 3 lysine 9 (H3K9) of gluconeogenic promoters, resulting in the increased association of CREB-CRTC2 and transcriptional activation of target gene ...
Synthesis of protein and peptide hormones
... Gene transcription (controlled by transcription factors) Processing of the initial mRNA transcript to ‘mature’ mRNA – excision of introns – and modifications of the 3’ and 5’ ends Translation of mRNA Bound to the polyribosomes of RER, the mRNA acts as a template for assembling amino acids by way ...
... Gene transcription (controlled by transcription factors) Processing of the initial mRNA transcript to ‘mature’ mRNA – excision of introns – and modifications of the 3’ and 5’ ends Translation of mRNA Bound to the polyribosomes of RER, the mRNA acts as a template for assembling amino acids by way ...
Document
... • Part of the GPCR C-terminus is tethered to the membrane by palmitoylation through a thioester linkage to a conserved Cys. • The G protein subunit has myristate attached via an amide linkage to an N-terminal Gly to effect membrane association. • The G protein subunit is incorporated into the me ...
... • Part of the GPCR C-terminus is tethered to the membrane by palmitoylation through a thioester linkage to a conserved Cys. • The G protein subunit has myristate attached via an amide linkage to an N-terminal Gly to effect membrane association. • The G protein subunit is incorporated into the me ...
Word file
... Supplementary Figure 2. EZH2 protein levels are elevated in metastatic prostate cancer independent of metastatic site. a, Tissue microarray analysis of EZH2 expression considered per patient. The mean EZH2 protein expression for the indicated prostate tissues is summarized using error bars with 95% ...
... Supplementary Figure 2. EZH2 protein levels are elevated in metastatic prostate cancer independent of metastatic site. a, Tissue microarray analysis of EZH2 expression considered per patient. The mean EZH2 protein expression for the indicated prostate tissues is summarized using error bars with 95% ...
SIRT3 - Safic-Alcan Italia
... is the third member of the mammalian sirtuin family. The SIRT3 gene encodes the protein SIRT3, which exhibits NAD+-dependent deacetylase activity. SIRT3 is a member of the sirtuin family of proteins, homologs to the yeast Sir2 protein. Members of the sirtuin family are characterized by a sirtuin c ...
... is the third member of the mammalian sirtuin family. The SIRT3 gene encodes the protein SIRT3, which exhibits NAD+-dependent deacetylase activity. SIRT3 is a member of the sirtuin family of proteins, homologs to the yeast Sir2 protein. Members of the sirtuin family are characterized by a sirtuin c ...
The Nobel Prize in Chemistry 2006 I
... necessary for transcription to start in our cells. These complexes are called general transcription factors and play an important role in the fine-tuning of transcription in eukaryotes. Finding all these five transcription factors required a great deal of painstaking work, purifying cell extracts in ...
... necessary for transcription to start in our cells. These complexes are called general transcription factors and play an important role in the fine-tuning of transcription in eukaryotes. Finding all these five transcription factors required a great deal of painstaking work, purifying cell extracts in ...
tissue-specificity of storage protein genes has evolved
... interact with the zinc-finger protein PBF (UEDA et al., 1994; VICENTE-CARBAJOSA et al., 1997). Therefore, the green callus cDNAs were also used to investigate PBF-gene expression with a specific primer pair flanking the 2-kb intron, which could be easily detected by size if the PCR product was ampli ...
... interact with the zinc-finger protein PBF (UEDA et al., 1994; VICENTE-CARBAJOSA et al., 1997). Therefore, the green callus cDNAs were also used to investigate PBF-gene expression with a specific primer pair flanking the 2-kb intron, which could be easily detected by size if the PCR product was ampli ...
a more thorough description of current interests.
... The other major goal of localization-induced metal function has been to engineer reversible metalligand with peripheral histidine, methionine, or cysteine near the binding site (Fig. 2, Path B). Many protein-protein interactions are potential therapeutic targets, but are considered “undrugable” beca ...
... The other major goal of localization-induced metal function has been to engineer reversible metalligand with peripheral histidine, methionine, or cysteine near the binding site (Fig. 2, Path B). Many protein-protein interactions are potential therapeutic targets, but are considered “undrugable” beca ...
Position Effect Variegation
... Advanced Molecular and Cellular Biology Bio4751 Spring 2003 Gary A. Bulla, PhD ...
... Advanced Molecular and Cellular Biology Bio4751 Spring 2003 Gary A. Bulla, PhD ...
TIBS Review - Structural Genomics Consortium
... successfully targeted in the past decade, resulting in approved drugs. However, it was only recently recognized that the protein domains that read acetyl-lysine—the evolutionarily conserved protein-protein interaction modules of the bromodomain (BRD) class [1]--can also be targeted. The bromo and ex ...
... successfully targeted in the past decade, resulting in approved drugs. However, it was only recently recognized that the protein domains that read acetyl-lysine—the evolutionarily conserved protein-protein interaction modules of the bromodomain (BRD) class [1]--can also be targeted. The bromo and ex ...
Dr. Anton Meinhart Department of Biomolecular
... Concomitant with eukaryotic transcription, RNA undergoes extensive modification. Nuclear processes, such as capping, splicing and cleavage / polyadenylation are necessary for producing a mature RNA. These RNA processing events take place in a dynamic interplay of individual transcription and process ...
... Concomitant with eukaryotic transcription, RNA undergoes extensive modification. Nuclear processes, such as capping, splicing and cleavage / polyadenylation are necessary for producing a mature RNA. These RNA processing events take place in a dynamic interplay of individual transcription and process ...
Riboswitches: the oldest regulatory system?
... RirA (from the Rrf2 family, with a rather different general consensus) took over their sites. 3. Iron-Rhodo boxes are recognized by IscR: directly testable ...
... RirA (from the Rrf2 family, with a rather different general consensus) took over their sites. 3. Iron-Rhodo boxes are recognized by IscR: directly testable ...
Epigenetics and its implications for Psychology
... in which it is used” (Keller, 2000, p. 69). Narrowly speaking, a gene can be defined from a biochemical point of view as a particular sequence of molecules known as “bases” or “nucleotides” (adenine, guanine, cytosine or thymine) that are linked to one another in a chain by chemical bonds constituti ...
... in which it is used” (Keller, 2000, p. 69). Narrowly speaking, a gene can be defined from a biochemical point of view as a particular sequence of molecules known as “bases” or “nucleotides” (adenine, guanine, cytosine or thymine) that are linked to one another in a chain by chemical bonds constituti ...
EPIgeneousTM Binding Domain Assays
... streptavidin-acceptor, to detect biotin-peptide. Reader domain interaction with the substrate brings the donor and acceptor dyes into close proximity, enabling FRET to occur upon light excitation. The specific signal at 665 nm is inhibited when a specific compound prevents the reader domain protein ...
... streptavidin-acceptor, to detect biotin-peptide. Reader domain interaction with the substrate brings the donor and acceptor dyes into close proximity, enabling FRET to occur upon light excitation. The specific signal at 665 nm is inhibited when a specific compound prevents the reader domain protein ...
Part 2
... a) Why is this a useful property of the Trp repressor? In the presence of environmental TRP, bacteria should not waste resources on its synthesis, but should respond to an absence of TRP by expressing the biosynthesis machinery required for its production. ...
... a) Why is this a useful property of the Trp repressor? In the presence of environmental TRP, bacteria should not waste resources on its synthesis, but should respond to an absence of TRP by expressing the biosynthesis machinery required for its production. ...
Slide 1 - Elsevier Store
... cyclase types I, III and VIII are stimulated by Ca 2+/calmodulin, an increase in cellular Ca2+ levels, which can result from either increased entry of Ca 2+ into the cell or increased release of Ca2+ from internal stores, would be expected to activate these enzymes. The actions of Ca 2+/calmodulin a ...
... cyclase types I, III and VIII are stimulated by Ca 2+/calmodulin, an increase in cellular Ca2+ levels, which can result from either increased entry of Ca 2+ into the cell or increased release of Ca2+ from internal stores, would be expected to activate these enzymes. The actions of Ca 2+/calmodulin a ...
Histone acetylation and deacetylation
Histone acetylation and deacetylation are the processes by which the lysine residues within the N-terminal tail protruding from the histone core of the nucleosome are acetylated and deacetylated as part of gene regulation. Histone acetylation and deacetylation are essential parts of gene regulation. These reactions are typically catalysed by enzymes with ""histone acetyltransferase"" (HAT) or ""histone deacetylase"" (HDAC) activity. Acetylation is the process where an acetyl functional group is transferred from one molecule (in this case, Acetyl-Coenzyme A) to another. Deacetylation is simply the reverse reaction where an acetyl group is removed from a molecule.Acetylated histones, octameric proteins that organize chromatin into nucleosomes and ultimately higher order structures, represent a type of epigenetic marker within chromatin. Acetylation removes the positive charge on the histones, thereby decreasing the interaction of the N termini of histones with the negatively charged phosphate groups of DNA. As a consequence, the condensed chromatin is transformed into a more relaxed structure that is associated with greater levels of gene transcription. This relaxation can be reversed by HDAC activity. Relaxed, transcriptionally active DNA is referred to as euchromatin. More condensed (tightly packed) DNA is referred to as heterochromatin. Condensation can be brought about by processes including deacetylation and methylation; the action of methylation is indirect and has no effect upon charge.