![Nuclear pore interactions with the genome](http://s1.studyres.com/store/data/006628559_1-8d9bcb85f294b15b0f7d9b0cfec120bc-300x300.png)
Nuclear pore interactions with the genome
... [1,2]. Here we review our current understanding of the molecular basis for gene targeting to the NPC or Nups and how this interaction impacts chromatin structure and transcription. ...
... [1,2]. Here we review our current understanding of the molecular basis for gene targeting to the NPC or Nups and how this interaction impacts chromatin structure and transcription. ...
Sten_Ilmjärv_Different Aspects of Gene Regulation
... initiating translation is a special initiator tRNA (tRNA), which carries the amino acid methionine. The first reactions involve the formation of an initiation complex consisting of methionyl-initiator tRNA bound to a 40S (measured in Svedbergs, in which a higher S value indicates a greater rate of s ...
... initiating translation is a special initiator tRNA (tRNA), which carries the amino acid methionine. The first reactions involve the formation of an initiation complex consisting of methionyl-initiator tRNA bound to a 40S (measured in Svedbergs, in which a higher S value indicates a greater rate of s ...
The NF- B Pathway
... include Ser468 and Thr505 but the responsible kinase(s) awaits identification. The phosphorylation of many of these sites is associated with an increase in the transcriptional activity of p65, and is accompanied by enhanced binding of p65 to coactivating acetylases such as CBP/p300. Interestingly, t ...
... include Ser468 and Thr505 but the responsible kinase(s) awaits identification. The phosphorylation of many of these sites is associated with an increase in the transcriptional activity of p65, and is accompanied by enhanced binding of p65 to coactivating acetylases such as CBP/p300. Interestingly, t ...
From profiles to function in epigenomics
... have been shown to be functionally involved in gene expression. The first chromatin mark to gain attention (and which is still the best-studied) was DNA methylation. Discovered in the late 1940s as a modification of cytosine bases24,118 it early on became a prime candidate for an epigenetic effector ...
... have been shown to be functionally involved in gene expression. The first chromatin mark to gain attention (and which is still the best-studied) was DNA methylation. Discovered in the late 1940s as a modification of cytosine bases24,118 it early on became a prime candidate for an epigenetic effector ...
MSc in Biochemistry, MSc in Biotechnology, MSc in Bioorganic
... chemical strategies to produce robust scaffolds where one can have a precise control over the location of crucial elements, such as functional groups for target recognition or metal ion binding. Therefore, these structures can render new, cheaper and more stable alternatives for therapeutic, chemica ...
... chemical strategies to produce robust scaffolds where one can have a precise control over the location of crucial elements, such as functional groups for target recognition or metal ion binding. Therefore, these structures can render new, cheaper and more stable alternatives for therapeutic, chemica ...
No Slide Title
... Problem 15.5 Consider a hypothetical regulatory scheme in which citrulline induces the production of urea cycle enzymes. Four genes (citA, citB, citC, citD) affecting the activity or regulation of the enzymes were analyzed by assaying the wild-type and mutant strains for argininosuccinate lyase act ...
... Problem 15.5 Consider a hypothetical regulatory scheme in which citrulline induces the production of urea cycle enzymes. Four genes (citA, citB, citC, citD) affecting the activity or regulation of the enzymes were analyzed by assaying the wild-type and mutant strains for argininosuccinate lyase act ...
Supplemental Data High Coding Density on the Largest
... The frequencies of dinucleotides for the megabase chromosome sequence were calculated taking into account the frequencies of each individual nucleotide and are given as the ratio of observed to expected frequencies, fobs/exp. We found both CpG and TpA dinucleotides to be underrepresented with respec ...
... The frequencies of dinucleotides for the megabase chromosome sequence were calculated taking into account the frequencies of each individual nucleotide and are given as the ratio of observed to expected frequencies, fobs/exp. We found both CpG and TpA dinucleotides to be underrepresented with respec ...
Mesoderm induction
... - chiral: curve clockwise (from base) = ‘handedness’ - mediate sliding between adjacent microtubules in cilia or flagella - cause cilia to rotate in a specific direction (clockwise) - monocilia (at Hensen’s node - mouse) generate oriented flow of signal molecules to the left side of the embryo - sig ...
... - chiral: curve clockwise (from base) = ‘handedness’ - mediate sliding between adjacent microtubules in cilia or flagella - cause cilia to rotate in a specific direction (clockwise) - monocilia (at Hensen’s node - mouse) generate oriented flow of signal molecules to the left side of the embryo - sig ...
Cooperative Function of Upstream and Core Domains of the Yeast
... promoter activity All of the work in this paper was done with a promoter fragment which extends from an SmaI site at position -216 upstream of transcription initiation to a Taq I site at position + 25 downstream of initiation. At the TaqI site a 16 or 26 bp linker has been inserted to facilitate dis ...
... promoter activity All of the work in this paper was done with a promoter fragment which extends from an SmaI site at position -216 upstream of transcription initiation to a Taq I site at position + 25 downstream of initiation. At the TaqI site a 16 or 26 bp linker has been inserted to facilitate dis ...
The reverse two
... detailed descriptions of the structure, function and control of biological systems in health and disease” Patterson & Aebersold Nat Genetics 33:S311 (2003) ...
... detailed descriptions of the structure, function and control of biological systems in health and disease” Patterson & Aebersold Nat Genetics 33:S311 (2003) ...
Gene Section Transcription 3) Atlas of Genetics and Cytogenetics
... domain is a transcriptional activation domain whose activity is enhanced by phosphorylation of serine 727. The STAT3 beta isoform (84 kDa) is missing this domain (1-715 + 7 unique amino acids resulting from frameshift) and is sometimes used as a dominant negative though there is also evidence that i ...
... domain is a transcriptional activation domain whose activity is enhanced by phosphorylation of serine 727. The STAT3 beta isoform (84 kDa) is missing this domain (1-715 + 7 unique amino acids resulting from frameshift) and is sometimes used as a dominant negative though there is also evidence that i ...
Transcription
... Concept The process of copying DNA to RNA by an enzyme called RNA polymerase (RNAP) The transfer of genetic information from DNA into RNA ...
... Concept The process of copying DNA to RNA by an enzyme called RNA polymerase (RNAP) The transfer of genetic information from DNA into RNA ...
Poster - Protein Information Resource
... Introduction: With the increasing volume of scientific literature available electronically, efficient text mining tools will greatly facilitate the extraction of information buried in free text and will assist in database annotation and scientific inquiry. Many methods, including natural language pr ...
... Introduction: With the increasing volume of scientific literature available electronically, efficient text mining tools will greatly facilitate the extraction of information buried in free text and will assist in database annotation and scientific inquiry. Many methods, including natural language pr ...
Novel domains and orthologues of eukaryotic
... MRC Functional Genetics Unit, Department of Human Anatomy and Genetics, University of Oxford, South Parks Road, Oxford OX1 3QX, UK Received May 28, 2002; Accepted July 2, 2002 ...
... MRC Functional Genetics Unit, Department of Human Anatomy and Genetics, University of Oxford, South Parks Road, Oxford OX1 3QX, UK Received May 28, 2002; Accepted July 2, 2002 ...
The Centromeric/Nucleolar Chromatin Protein ZFP
... ;50%. In addition, human ZFP-37 contains a longer NH2terminal region upstream of the KRAB domain. This raises the question of whether these less conserved regions serve the same function in mouse and man. However, alignment of the ZFP-37 linker, located between KRAB and zinc finger domains, to the S ...
... ;50%. In addition, human ZFP-37 contains a longer NH2terminal region upstream of the KRAB domain. This raises the question of whether these less conserved regions serve the same function in mouse and man. However, alignment of the ZFP-37 linker, located between KRAB and zinc finger domains, to the S ...
An efficient immunodetection method for histone modifications in
... (Figure 2) were fixed in formaldehyde. In most of the protocols, the fixation step must be optimized according to the type of plant or tissue [23,28]. In the protocol described here, the sample fixation was carried out with final concentration of 3.7% formaldehyde, providing good results. In additio ...
... (Figure 2) were fixed in formaldehyde. In most of the protocols, the fixation step must be optimized according to the type of plant or tissue [23,28]. In the protocol described here, the sample fixation was carried out with final concentration of 3.7% formaldehyde, providing good results. In additio ...
Chapter 17
... mRNA is the “messenger” or vehicle that carries the genetic information from the DNA to the protein synthesizing machinery. RNA polymerase pries apart the DNA and joins RNA nucleotides together in the 5’-->3’ direction (adding, again, to the free 3’ end). RNA polymerase is just like DNA polymerase, ...
... mRNA is the “messenger” or vehicle that carries the genetic information from the DNA to the protein synthesizing machinery. RNA polymerase pries apart the DNA and joins RNA nucleotides together in the 5’-->3’ direction (adding, again, to the free 3’ end). RNA polymerase is just like DNA polymerase, ...
... treatments and seven replicates in four periods of 28 days/each was used. The treatments were: Control - Formulated according to the nutritional requirements proposed in the strain, containing 16.92% crude protein, 0.750% digestible lysine. Treatments 1 to 5, with crude protein levels of 14% and 0.6 ...
8.4 Transcription
... translated to form a protein. – Ribosomal RNA (rRNA) forms part of ribosomes where proteins are made. – Transfer RNA (tRNA) brings amino acids from the cytoplasm to a ribosome. ...
... translated to form a protein. – Ribosomal RNA (rRNA) forms part of ribosomes where proteins are made. – Transfer RNA (tRNA) brings amino acids from the cytoplasm to a ribosome. ...
The Bcl-3 oncoprotein acts as a bridging factor between NF
... conditions. In contrast, expression of the other Bcl-3 interacting proteins led to a pronounced increase in Bcl-3-associated p50 dimers. The strongest increase in p50 DNA-binding activity was achieved by Jab1, followed by Pirin and Tip60. Thus, expression of Jab1, Tip60 or Pirin enhance association ...
... conditions. In contrast, expression of the other Bcl-3 interacting proteins led to a pronounced increase in Bcl-3-associated p50 dimers. The strongest increase in p50 DNA-binding activity was achieved by Jab1, followed by Pirin and Tip60. Thus, expression of Jab1, Tip60 or Pirin enhance association ...
transcription and rna
... Conserved sequences in prokaryotic promoters Conserved sequences: similar nucleotide sequence regions among promoters Point of reference for gene: transcription start point (nucleotide +1) Minus 10 sequence (TATAAT) T and A pairs facilitate strand separation Site of initial DNA strand separation Min ...
... Conserved sequences in prokaryotic promoters Conserved sequences: similar nucleotide sequence regions among promoters Point of reference for gene: transcription start point (nucleotide +1) Minus 10 sequence (TATAAT) T and A pairs facilitate strand separation Site of initial DNA strand separation Min ...
Histone acetylation and deacetylation
![](https://commons.wikimedia.org/wiki/Special:FilePath/Nucleosome_1KX5_colour_coded.png?width=300)
Histone acetylation and deacetylation are the processes by which the lysine residues within the N-terminal tail protruding from the histone core of the nucleosome are acetylated and deacetylated as part of gene regulation. Histone acetylation and deacetylation are essential parts of gene regulation. These reactions are typically catalysed by enzymes with ""histone acetyltransferase"" (HAT) or ""histone deacetylase"" (HDAC) activity. Acetylation is the process where an acetyl functional group is transferred from one molecule (in this case, Acetyl-Coenzyme A) to another. Deacetylation is simply the reverse reaction where an acetyl group is removed from a molecule.Acetylated histones, octameric proteins that organize chromatin into nucleosomes and ultimately higher order structures, represent a type of epigenetic marker within chromatin. Acetylation removes the positive charge on the histones, thereby decreasing the interaction of the N termini of histones with the negatively charged phosphate groups of DNA. As a consequence, the condensed chromatin is transformed into a more relaxed structure that is associated with greater levels of gene transcription. This relaxation can be reversed by HDAC activity. Relaxed, transcriptionally active DNA is referred to as euchromatin. More condensed (tightly packed) DNA is referred to as heterochromatin. Condensation can be brought about by processes including deacetylation and methylation; the action of methylation is indirect and has no effect upon charge.