Mitogen-activated protein kinases and cardiomyocyte hypertrophy
... activate tyrosine and threonine residues in the activation loop of the MAPKs. Most physiological substrates of the MAPKs possess specific binding sites for MAPKs that allow strong interactions with selectivity for MAPK subfamilies [3]. MAPKs, in turn, also possess complementary docking sites, which ...
... activate tyrosine and threonine residues in the activation loop of the MAPKs. Most physiological substrates of the MAPKs possess specific binding sites for MAPKs that allow strong interactions with selectivity for MAPK subfamilies [3]. MAPKs, in turn, also possess complementary docking sites, which ...
Long Noncoding RNAs Add Another Layer to Pre
... many of them are conserved, their expression is developmentally regulated, and they have tissue-specific expression patterns (Mercer et al., 2009). Furthermore, several long ncRNAs appear to be misregulated in diseases, such as cancer. Loci encoding long ncRNAs often overlap with or are interspersed ...
... many of them are conserved, their expression is developmentally regulated, and they have tissue-specific expression patterns (Mercer et al., 2009). Furthermore, several long ncRNAs appear to be misregulated in diseases, such as cancer. Loci encoding long ncRNAs often overlap with or are interspersed ...
Epigenetic Control of Germline Development
... in development. Examples of epigenetic phenomena include imprinting, X chromosome dosage compensation, and gene silencing. X chromosome dosage compensation mechanisms, for example, utilize chromatin modifications and noncoding RNAs to heritably inactivate one X chromosome in female placental mammals ...
... in development. Examples of epigenetic phenomena include imprinting, X chromosome dosage compensation, and gene silencing. X chromosome dosage compensation mechanisms, for example, utilize chromatin modifications and noncoding RNAs to heritably inactivate one X chromosome in female placental mammals ...
Analysis of hepatocyte nuclear factor
... revealed that they are structurally complex, consisting of multiple DNA binding sites recognized by distinct families of liverenriched transcription factors (1). The combinatorial action of these factors on multiple DNA sites is required for the activation of transcription and plays a role in mainta ...
... revealed that they are structurally complex, consisting of multiple DNA binding sites recognized by distinct families of liverenriched transcription factors (1). The combinatorial action of these factors on multiple DNA sites is required for the activation of transcription and plays a role in mainta ...
Mapping functional regions of the segment
... protein to identify the regions involved in DNA-binding and transcriptional activation. Our data suggest that only the zinc fingers are required for high affinity, specific DNA-binding. Transcriptional activation was not affected by deletion of the C-terminal tail of the protein. In contrast, deleti ...
... protein to identify the regions involved in DNA-binding and transcriptional activation. Our data suggest that only the zinc fingers are required for high affinity, specific DNA-binding. Transcriptional activation was not affected by deletion of the C-terminal tail of the protein. In contrast, deleti ...
HGD Gene Expression
... “The central dogma of molecular biology deals with the detailed residue-by-residue transfer of sequential information. It states that such information cannot be transferred from protein to either protein or nucleic acid” (Francis Crick, Nature, 1970) . Pathway of information flow from "DNA to RNA to ...
... “The central dogma of molecular biology deals with the detailed residue-by-residue transfer of sequential information. It states that such information cannot be transferred from protein to either protein or nucleic acid” (Francis Crick, Nature, 1970) . Pathway of information flow from "DNA to RNA to ...
With or Without them: Essential Roles of Cofactors in ES Cells
... Hui Ma1#, Jin Rong Ow1#, Xi Chen2* and Qiang Wu1* Department of Biochemistry, Yong Loo Lin School of Medicine, National University of Singapore, 8 Medical Drive, Singapore 117597 Department of Immunology and Infectious Diseases, Harvard School of Public Health, Boston, Massachusetts 02115, USA ...
... Hui Ma1#, Jin Rong Ow1#, Xi Chen2* and Qiang Wu1* Department of Biochemistry, Yong Loo Lin School of Medicine, National University of Singapore, 8 Medical Drive, Singapore 117597 Department of Immunology and Infectious Diseases, Harvard School of Public Health, Boston, Massachusetts 02115, USA ...
Document
... Transcription occurs in two major steps. 2. Nucleotides pair with their complementary bases on one strand of the DNA. – RNA polymerase bonds the nucleotides together. – The DNA helix winds again as the gene is transcribed. ...
... Transcription occurs in two major steps. 2. Nucleotides pair with their complementary bases on one strand of the DNA. – RNA polymerase bonds the nucleotides together. – The DNA helix winds again as the gene is transcribed. ...
viral networks
... http://www.dnatube.com/video/993/Plasmid• Only need the sequenced genome (or sequence of Cloning interest) • Scalable, its possible to screen for interactions among many proteins creating a more high-throughput screen (ex. viral genome) • Protein/polypeptides can be from various sources; eukaryotes, ...
... http://www.dnatube.com/video/993/Plasmid• Only need the sequenced genome (or sequence of Cloning interest) • Scalable, its possible to screen for interactions among many proteins creating a more high-throughput screen (ex. viral genome) • Protein/polypeptides can be from various sources; eukaryotes, ...
E. coli
... Unlike eukaryotic systems where transcription and translation occur sequentially, in E. coli, transcription and translation occur simultaneously within the cell In vitro E. coli translation systems are thus performed the same way, coupled, in the same tube under the same reaction conditions. During ...
... Unlike eukaryotic systems where transcription and translation occur sequentially, in E. coli, transcription and translation occur simultaneously within the cell In vitro E. coli translation systems are thus performed the same way, coupled, in the same tube under the same reaction conditions. During ...
Experimental Approaches to Protein–Protein Interactions
... carry out similar functions in different complexes [8]. This straightforward logic is, however, obscured by frequent examples of redundancy or non-orthologous replacement. This is not unexpected, given that the components of complexes arose as a result of evolutionary processes. There are clearly st ...
... carry out similar functions in different complexes [8]. This straightforward logic is, however, obscured by frequent examples of redundancy or non-orthologous replacement. This is not unexpected, given that the components of complexes arose as a result of evolutionary processes. There are clearly st ...
Virtual Lac Operon Activity[1].
... Why do some genes have multiple pathways controlling gene expression? Control of transcription is often a complex process. The presence of one molecule may prevent transcription while the presence of a different molecule may stimulate transcription but only if the first molecule is not present. Mult ...
... Why do some genes have multiple pathways controlling gene expression? Control of transcription is often a complex process. The presence of one molecule may prevent transcription while the presence of a different molecule may stimulate transcription but only if the first molecule is not present. Mult ...
DNA methylation
... Polycomb group PRC2 complex containing Jarid2 shown in red), at promoters. Additionally, bivalent genes have non-methylated CpG DNA regions (non-mCpG), and possess repressive H2AK119Ub1 marks (deposited by Ring1a/b ubiquitin E3 ligases within a PRC1 complex shown in dark blue), at the promoter regio ...
... Polycomb group PRC2 complex containing Jarid2 shown in red), at promoters. Additionally, bivalent genes have non-methylated CpG DNA regions (non-mCpG), and possess repressive H2AK119Ub1 marks (deposited by Ring1a/b ubiquitin E3 ligases within a PRC1 complex shown in dark blue), at the promoter regio ...
Text - Enlighten - University of Glasgow
... rise when polyamine levels rise, thus reducing ODC levels causing synthesis of new polyamines to diminish. Further control is provided by a catalytically dead paralogue of ODC, an antizyme inhibitor that binds and neutralizes antizyme (Mangold, 2006). AdoMetDC is also regulated at the transcriptiona ...
... rise when polyamine levels rise, thus reducing ODC levels causing synthesis of new polyamines to diminish. Further control is provided by a catalytically dead paralogue of ODC, an antizyme inhibitor that binds and neutralizes antizyme (Mangold, 2006). AdoMetDC is also regulated at the transcriptiona ...
MODified™ Protein Domain Binding Kit Manual
... and chromatin remodeling by interacting with specific histone PTMs. Some common chromatin remodeling protein domains include bromodomains, chromodomains and tudor domains to name a few. The MODified™ Protein Domain Binding Kit is designed to be used in combination with Active Motif’s MODified Histon ...
... and chromatin remodeling by interacting with specific histone PTMs. Some common chromatin remodeling protein domains include bromodomains, chromodomains and tudor domains to name a few. The MODified™ Protein Domain Binding Kit is designed to be used in combination with Active Motif’s MODified Histon ...
Chapter 31 - Department of Chemistry [FSU]
... • CAP structure may protect against RNase degradation • CAP structure also important in binding orientation on ribosome ...
... • CAP structure may protect against RNase degradation • CAP structure also important in binding orientation on ribosome ...
control of gene expression
... • i.e. there were 2 genes, one coding for the structure and one coding for the production of the protein (the mutation was in the one coding for the production) ...
... • i.e. there were 2 genes, one coding for the structure and one coding for the production of the protein (the mutation was in the one coding for the production) ...
Leukaemia Section t(10;11)(p12;q23) KMT2A/NEBL Atlas of Genetics and Cytogenetics in Oncology and Haematology
... A. Left: Long-distance inverse polymerase chain reaction (LDI-PCR) analysis of both derivatives using genomic DNA. Lane M, size marker; lane 1, LDI-PCR analysis of der(11) showing the wild-type (wt) band and the der(11) band (asterisk); lane 2, LDIPCR analysis of der(10) showing the wt band and the ...
... A. Left: Long-distance inverse polymerase chain reaction (LDI-PCR) analysis of both derivatives using genomic DNA. Lane M, size marker; lane 1, LDI-PCR analysis of der(11) showing the wild-type (wt) band and the der(11) band (asterisk); lane 2, LDIPCR analysis of der(10) showing the wt band and the ...
Supplementary Information (doc 4960K)
... determine and remove the exact non-specific probe(s). RNA extraction and processing for hybridization to the microarray RNA was extracted using Ambion® RiboPureTM kit (Life Technologies, Grand Island, NY) with modifications. The filter was removed from the Sterivex cartridge and placed into a 2.0 mL ...
... determine and remove the exact non-specific probe(s). RNA extraction and processing for hybridization to the microarray RNA was extracted using Ambion® RiboPureTM kit (Life Technologies, Grand Island, NY) with modifications. The filter was removed from the Sterivex cartridge and placed into a 2.0 mL ...
Structure and dynamics of the crenarchaeal nucleoid
... (atomic force microscopy) studies have shown that all three proteins form compacted protein–DNA complexes at low or intermediate stoichiometries and that Alba forms open (circular) protein–DNA complexes at high protein/DNA ratios [15,19,24]. The ability of Alba to bridge two DNA fragments, as observ ...
... (atomic force microscopy) studies have shown that all three proteins form compacted protein–DNA complexes at low or intermediate stoichiometries and that Alba forms open (circular) protein–DNA complexes at high protein/DNA ratios [15,19,24]. The ability of Alba to bridge two DNA fragments, as observ ...
Down regulation of gene-expression by N
... stably transfected with the N-myc-ER chimera (SKNAS-NmycER) was used for additional analysis of genes down regulated by N-myc. In both SHEP-21N and SKNAS-NmycER we could discriminate between genes which were regulated fast (within 1-2 days) and genes which were regulated slow (after 45 days), pointi ...
... stably transfected with the N-myc-ER chimera (SKNAS-NmycER) was used for additional analysis of genes down regulated by N-myc. In both SHEP-21N and SKNAS-NmycER we could discriminate between genes which were regulated fast (within 1-2 days) and genes which were regulated slow (after 45 days), pointi ...
Full PDF - American Journal of Physiology
... chain into a neutral residue. This modification results in a structural change of the histone tail, which in turn alters the interaction between histones and DNA together with the associations with the nucleosomes. These alterations result in a rather open chromatin (euchromatin) structure that is t ...
... chain into a neutral residue. This modification results in a structural change of the histone tail, which in turn alters the interaction between histones and DNA together with the associations with the nucleosomes. These alterations result in a rather open chromatin (euchromatin) structure that is t ...
activator - Cardinal Newman High School
... Initiation of Translation • The initiation of translation of selected mRNAs can be blocked by regulatory proteins that bind to sequences or structures of the ...
... Initiation of Translation • The initiation of translation of selected mRNAs can be blocked by regulatory proteins that bind to sequences or structures of the ...
Identification of TF Binding Sites in Promoter Databases final version
... preserved 85 amino acid residues. This conserved functional domain enables binding to a purine-rich DNA sequence with central 5‟-GGAA/T-3‟ core sequence; DNA binding inhibition and transactivation. ETS family TFs can be subdivided into 30 protein members and one of its members is the Pea3 group prot ...
... preserved 85 amino acid residues. This conserved functional domain enables binding to a purine-rich DNA sequence with central 5‟-GGAA/T-3‟ core sequence; DNA binding inhibition and transactivation. ETS family TFs can be subdivided into 30 protein members and one of its members is the Pea3 group prot ...
Histone acetylation and deacetylation
Histone acetylation and deacetylation are the processes by which the lysine residues within the N-terminal tail protruding from the histone core of the nucleosome are acetylated and deacetylated as part of gene regulation. Histone acetylation and deacetylation are essential parts of gene regulation. These reactions are typically catalysed by enzymes with ""histone acetyltransferase"" (HAT) or ""histone deacetylase"" (HDAC) activity. Acetylation is the process where an acetyl functional group is transferred from one molecule (in this case, Acetyl-Coenzyme A) to another. Deacetylation is simply the reverse reaction where an acetyl group is removed from a molecule.Acetylated histones, octameric proteins that organize chromatin into nucleosomes and ultimately higher order structures, represent a type of epigenetic marker within chromatin. Acetylation removes the positive charge on the histones, thereby decreasing the interaction of the N termini of histones with the negatively charged phosphate groups of DNA. As a consequence, the condensed chromatin is transformed into a more relaxed structure that is associated with greater levels of gene transcription. This relaxation can be reversed by HDAC activity. Relaxed, transcriptionally active DNA is referred to as euchromatin. More condensed (tightly packed) DNA is referred to as heterochromatin. Condensation can be brought about by processes including deacetylation and methylation; the action of methylation is indirect and has no effect upon charge.