Pure Culture - IRSC Biology Department
... Structural Stains: stains only one part of a cell so it can be distinguished from the rest of the cell (ex: flagellar) ...
... Structural Stains: stains only one part of a cell so it can be distinguished from the rest of the cell (ex: flagellar) ...
Gram-staining - BMC Dentists 2011
... the slide by placing it on Hot plate for 3-5 minutes. Do not allow the stain to boil and dry but it should only evaporate. By this way stain easily goes into the cell. ...
... the slide by placing it on Hot plate for 3-5 minutes. Do not allow the stain to boil and dry but it should only evaporate. By this way stain easily goes into the cell. ...
zog_data2
... In the radio it looks quite different, as can be seen in these three images, taken at different resolutions. It seems to have a jet of relativistic particles squirting out in both directions. ...
... In the radio it looks quite different, as can be seen in these three images, taken at different resolutions. It seems to have a jet of relativistic particles squirting out in both directions. ...
Lab 4
... • Learn methods of differential staining: – Use two or more stains and categorize cells into groups – Gram Staining – Separates bacteria in two different groups – Gram positive and Gram negative – Important first test for bacterial identification ...
... • Learn methods of differential staining: – Use two or more stains and categorize cells into groups – Gram Staining – Separates bacteria in two different groups – Gram positive and Gram negative – Important first test for bacterial identification ...
KUMASI™ stabilized colloidal stain for polyacrylamide gels
... Compared to conventional Coomassie stains which generate chemically hazardous waste streams, the new generation of “ecologically friendly” Coomassie stains exhibit significantly decreased sensitivity. The solution is the KUMASI stabilized colloidal stain which can be reused at least four times, redu ...
... Compared to conventional Coomassie stains which generate chemically hazardous waste streams, the new generation of “ecologically friendly” Coomassie stains exhibit significantly decreased sensitivity. The solution is the KUMASI stabilized colloidal stain which can be reused at least four times, redu ...
ZIEHL-NEELSEN for acid-fast bacteria 04 – 110802
... Read with attention the information written on the label (dangerous symbols, risks and safety phrases). Consult always the safety data sheet where the information about the risks of the preparation, precautionary measures during use, first aid and disposal are available. Do not use if primary packag ...
... Read with attention the information written on the label (dangerous symbols, risks and safety phrases). Consult always the safety data sheet where the information about the risks of the preparation, precautionary measures during use, first aid and disposal are available. Do not use if primary packag ...
Document
... • Stains are salts that consist of a positive and negative ion ─ Acidic dye: the chromophore is an anion ─ Basic dye: the chromophore is a cation Crystal violet: ...
... • Stains are salts that consist of a positive and negative ion ─ Acidic dye: the chromophore is an anion ─ Basic dye: the chromophore is a cation Crystal violet: ...
Coomassie Brilliant Blue
... The Coomassie Brilliant Blue dye which is commonly used in SDS-PAGE, was first described by the German scientist Volker Neuhoff. The dye gets its name from the African city Kumasi, formerly Coomassie, a city in central Ghana. Currently, there are two kinds of the Coomassie dyes: R-250 and G-250. Coo ...
... The Coomassie Brilliant Blue dye which is commonly used in SDS-PAGE, was first described by the German scientist Volker Neuhoff. The dye gets its name from the African city Kumasi, formerly Coomassie, a city in central Ghana. Currently, there are two kinds of the Coomassie dyes: R-250 and G-250. Coo ...
microbiology introduction
... -lactose fermentation produces acids, which lower the pH and encourages dye absorption by the colonies, which are now colored purpleblack and display "nucleated colonies“- colonies with dark centers -lactose non-fermenters may increase the pH by deamination of proteins which ensures that the dye is ...
... -lactose fermentation produces acids, which lower the pH and encourages dye absorption by the colonies, which are now colored purpleblack and display "nucleated colonies“- colonies with dark centers -lactose non-fermenters may increase the pH by deamination of proteins which ensures that the dye is ...
Indirect (negative) staining
... Indirect (negative) staining • To heighten the contrast between bacteria and the background, use is made of electron-dense "stains". • These are usually compounds of heavy metals of high atomic number, that serve to scatter the electrons from regions covered with the stain. • If bacterial particles ...
... Indirect (negative) staining • To heighten the contrast between bacteria and the background, use is made of electron-dense "stains". • These are usually compounds of heavy metals of high atomic number, that serve to scatter the electrons from regions covered with the stain. • If bacterial particles ...
S6. Using Yeast to Make Scientists-Introduction to
... Model organisms are used to study biological processes, with the hopes of extrapolating results to humans. They are less expensive to maintain, reproduce faster, and raise fewer ethical questions. https://biology.uiowa.edu/model-organisms ...
... Model organisms are used to study biological processes, with the hopes of extrapolating results to humans. They are less expensive to maintain, reproduce faster, and raise fewer ethical questions. https://biology.uiowa.edu/model-organisms ...
Topic 2: Microscopy and Staining Measurement of Microorganisms
... to high lipid content of a wall, we must use harsher treatment. Instead they are heated with basic fuchsin and phenol (Ziehl-Neelson method). They retain the red colour of the dye when rinsed in 3% solution of HCl in ethanol. Non-acid fast bacteria are decolourised and are stained blue by methylene ...
... to high lipid content of a wall, we must use harsher treatment. Instead they are heated with basic fuchsin and phenol (Ziehl-Neelson method). They retain the red colour of the dye when rinsed in 3% solution of HCl in ethanol. Non-acid fast bacteria are decolourised and are stained blue by methylene ...
in-vivo-staining - kehsscience.org
... color(s), their form (morphology) or position within a cell or tissue can be readily seen and studied. The usual purpose is to reveal cytological details that might otherwise not be apparent; however, staining can also reveal where certain chemicals or specific chemical reactions are taking place wi ...
... color(s), their form (morphology) or position within a cell or tissue can be readily seen and studied. The usual purpose is to reveal cytological details that might otherwise not be apparent; however, staining can also reveal where certain chemicals or specific chemical reactions are taking place wi ...
PowerPoint file
... Electron wavelength ~ 100,000 x smaller than visible light wavelength Specimens may be stained with heavy metal salts Two types of EMs:? ...
... Electron wavelength ~ 100,000 x smaller than visible light wavelength Specimens may be stained with heavy metal salts Two types of EMs:? ...
Bio 181: Blue/White screening (pBLU) A central problem of cloning
... Bacteria carrying empty pBLU vs bacteria carrying pBLU+PCRproduct (the desired clones): All of these cells will be able to grow on ampicillin. To identify desired clones, use Blue/White screening. ...
... Bacteria carrying empty pBLU vs bacteria carrying pBLU+PCRproduct (the desired clones): All of these cells will be able to grow on ampicillin. To identify desired clones, use Blue/White screening. ...
LAB 3 - Home - KSU Faculty Member websites
... envelope (rather than the basic gram- related properties). ...
... envelope (rather than the basic gram- related properties). ...
ZIEHL-NEELSEN for acid-fast bacteria 04 – 111802/L
... Berg JW. Chemistry of acid-fastness Proc Soc Exp Biol Med 1953; 84: 196-198 Lillie RD. Acetic methylene blue counterstain in staining tissue in acid fast bacilli. Stain Technol ...
... Berg JW. Chemistry of acid-fastness Proc Soc Exp Biol Med 1953; 84: 196-198 Lillie RD. Acetic methylene blue counterstain in staining tissue in acid fast bacilli. Stain Technol ...
BASIC TECHNIQUES Preparation of histological sections In order to
... 1. Hematoxylin and Eosin (H&E) This is the most commonly used staining technique for histological and histopathological sections. The Hematoxylin is a basic dye that stains acidic components of cells a blue color. This characteristic is known as basophilia. Hematoxylin stains the nuclei of cells, a ...
... 1. Hematoxylin and Eosin (H&E) This is the most commonly used staining technique for histological and histopathological sections. The Hematoxylin is a basic dye that stains acidic components of cells a blue color. This characteristic is known as basophilia. Hematoxylin stains the nuclei of cells, a ...
Gram Stain
... Negative staining provides a more detailed assessment of a microbe's morphology than simple staining does because the background (rather than the microbe) is stained. This prevents the staining procedure from causing any distortion to the microbe's ultrastructure. It also makes the outline of the ce ...
... Negative staining provides a more detailed assessment of a microbe's morphology than simple staining does because the background (rather than the microbe) is stained. This prevents the staining procedure from causing any distortion to the microbe's ultrastructure. It also makes the outline of the ce ...
The Microscope: Window on an Invisible Realm
... Acid Fast organisms – carbol fuchsin is retained in the lipid-rich cell wall – stain red Non-acid-fast organisms – decolorize with the acid – accept the counterstain – stain blue Special Stains - See Fig 3.21 Negative stains for Capsules Capsules are virulence factors ; help bacteria cause dis ...
... Acid Fast organisms – carbol fuchsin is retained in the lipid-rich cell wall – stain red Non-acid-fast organisms – decolorize with the acid – accept the counterstain – stain blue Special Stains - See Fig 3.21 Negative stains for Capsules Capsules are virulence factors ; help bacteria cause dis ...
Acid-Fast Stain
... Acid Fast bacteria, such as Mycobacterium tuberculosis and Mycobacterium leprae, contain long chain fatty acids (mycolic acids) in their cell walls. These cells are impervious to methylene blue and other basic dyes. The carbolfuchsin dye penetrates the cell wall and stains the bacteria. You must eit ...
... Acid Fast bacteria, such as Mycobacterium tuberculosis and Mycobacterium leprae, contain long chain fatty acids (mycolic acids) in their cell walls. These cells are impervious to methylene blue and other basic dyes. The carbolfuchsin dye penetrates the cell wall and stains the bacteria. You must eit ...
Alcian blue stain
Alcian blue or alcian blue (/ˈælʃən/) is any member of a family of polyvalent basic dyes, of which the Alcian blue 8G (also called Ingrain blue 1, and C.I. 74240, formerly called Alcian blue 8GX from the name of a batch of an ICI product) has been historically the most common and the most reliable member. It is used to stain acidic polysaccharides such as glycosaminoglycans in cartilages and other body structures, some types of mucopolysaccharides, sialylated glycocalyx of cells etc. For many of these targets it is one of the most widely used cationic dyes for both light and electron microscopy. Use of alcian blue has historically been a popular staining method in histology especially for light microscopy in paraffin embedded sections and in semithin resin sections. The tissue parts that specifically stain by this dye become blue to bluish-green after staining and are called ""Alcianophilic"" (akin to ""eosinophilic"" or ""sudanophilic""). Alcian blue staining can be combined with H&E staining, PAS staining and van Gieson staining methods. Alcian blue can be used to quantitate acidic glycans both in microspectrophotometric quantitation in solution or for staining glycoproteins in polyacrylamide gels or on western blots. Biochemists had used it to assay acid polysaccharides in urine since the 1960s for diagnosis of diseases like mucopolysaccharidosis but from 1970's, partly due to lack of availability of Alcian and partly due to length and tediousness of the procedure, alternative methods had to be developed e.g. Dimethyl methylene blue (DMB) method.Prof. J. E. Scott, the first person outside the dye Industry to crack the chemical secret of this dye comments:""Probably no other dyestuff has been applied to such wide variety of problems in biology and medicine. On the other hand, no other dyestuff had such a chequered history as AB.""In addition to its wide use as a stain Alcian blue has also been used in other diverse applications e.g. gelling agent for lubricating fluids, modifiers for electrodes, charged coating agents etc.