Download ZIEHL-NEELSEN for acid-fast bacteria 04 – 111802/L

ZIEHL-NEELSEN
for acid-fast bacteria
04 – 111802/L
IVD In-vitro diagnostic medical device
|
CND Code: W01030799
number of tests ........................... /
procedure time ............................ 45 minutes
product validity ............................ 2 years
storage temperature ..................... 15 - 25 °C
complementary equipment........... not requested
Expected aim
Product for the preparation of cyto-histological samples for optical microscopy.
Application
To show pathogenic mycobacteria (especially Koch’s bacillus) in histologic sections, sputum smears and
culture smears.
Principle
Acid resistance is a particular dyeing features of myco-bacteria. Once they have been stained with
carbol fuchsin they have the capacity to remain red even if they undergo strong decolorizations. Acid
resistance is usually ascribed to the special composition of mycobacteria’s cell walls, which are very
rich in lipides.
Method
1)
2)
3)
4)
5)
6)
7)
8)
9)
10)
Results
Koch’ s bacillus and other acid resistant elements ..............................................................................Red
Background .......................................................................................................................................... Blue
Reagents
A)
B)
C)
D)
Warning and
precaution
The product must be used exclusively by specialized technical operators.
The product is classified as hazardous.
Read with attention the information written on the label (dangerous symbols, risks and safety phrases).
Consult always the safety data sheet where the information about the risks of the preparation,
precautionary measures during use, first aid and disposal are available. Do not use if primary packaging
is damaged.
Storage
Store the preparation at room temperature. Keep the containers tightly closed.
Stability
After the first opening, the product is usable until the expiry date, if correctly stored.
Disposal
Hazardous preparation: observe all state and local environmental regulations regarding waste disposal.
Bring sections to distilled water.
Immerse sections in reagent A: leave to act 10 minutes.
Rinse in distilled water.
Immerse sections in reagent B: leave to act 30 minutes.
Wash in distilled water and dry the slide with filter paper.
Immerse sections in C: leave to act 1 minute.
Rinse 3 minutes in tap water.
Immerse sections in reagent D: leave to act 1 minute.
Wash in distilled water and blue in running tap water for 5 minutes.
Dehydrate through ascending alcohols, xylene and mount.
Periodic acid solution.............................................................................................................. 1000 ml
Carbolfuchsin solution ............................................................................................................ 1000 ml
Acid differentiation buffer ........................................................................................................ 1000 ml
Methylene blue solution .......................................................................................................... 1000 ml
Producer: Bio-Optica Milano s.p.a.
Page 1 di 2
Bio Optica Milano s.p.a.
via San Faustino 58 • I - 20134 Milano
tel. +39 02212713.1 • fax +39 022153000
www.bio-optica.it
References
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•
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Berg JW. Acid-fastness as a histochemical test J Histo-chem Cytochem 1953; 1: 436-441
Berg JW. Chemistry of acid-fastness Proc Soc Exp Biol Med 1953; 84: 196-198
Lillie RD. Acetic methylene blue counterstain in staining tissue in acid fast bacilli. Stain Technol
1944; 19-45
Date of issue: June 2013
Producer: Bio-Optica Milano s.p.a.
Page 2 di 2
Bio Optica Milano s.p.a.
via San Faustino 58 • I - 20134 Milano
tel. +39 02212713.1 • fax +39 022153000
www.bio-optica.it