A modified Coomassie Brilliant Blue staining method at nanogram
... for its ability to visualize and quantify relative changes in abundances of proteins (Patton 2000). However, the fluorescence used in this method can quench rapidly and the dyes are expensive. Additionally, special hardware and software for protein assessment are needed (Patton 2000). Silver stainin ...
... for its ability to visualize and quantify relative changes in abundances of proteins (Patton 2000). However, the fluorescence used in this method can quench rapidly and the dyes are expensive. Additionally, special hardware and software for protein assessment are needed (Patton 2000). Silver stainin ...
Preview Sample 2
... nonliquefiable solid. Artificial media are classified by their chemical composition as either defined or complex, depending on whether the exact chemical composition is known. Enriched, selective, differential, transport, assay, and enumerating media are all examples of media designed for specific p ...
... nonliquefiable solid. Artificial media are classified by their chemical composition as either defined or complex, depending on whether the exact chemical composition is known. Enriched, selective, differential, transport, assay, and enumerating media are all examples of media designed for specific p ...
(4) staining
... - In a routine histological technique, stained tissue sections are mounted in a mounting medium soluble in an organic solvent, such as xylene. - So tissue sections must be dehydrated in a serious of gradually increasing concentration of alcohol before passing to xylene. - If tissue does not cleared ...
... - In a routine histological technique, stained tissue sections are mounted in a mounting medium soluble in an organic solvent, such as xylene. - So tissue sections must be dehydrated in a serious of gradually increasing concentration of alcohol before passing to xylene. - If tissue does not cleared ...
efficiency of complex formation between aluminium and morin or
... evaluate the possibility to use the limitations to predict the binding forms of Al in different regions of the root tip by using different dyes or by simultaneous staining. Under optimized conditions of dye concentration for sensitivity and maximum fluorescence intensity, the Al concentration was va ...
... evaluate the possibility to use the limitations to predict the binding forms of Al in different regions of the root tip by using different dyes or by simultaneous staining. Under optimized conditions of dye concentration for sensitivity and maximum fluorescence intensity, the Al concentration was va ...
The Size of It All
... mesh grid where a beam of electrons pass through to an electromagnetic objective lens, which magnifies the image. The final image is seen as light and dark areas, referred to as a transmission electron micrograph. • Disadvantages: 1. requires a very thin slice of the specimen ...
... mesh grid where a beam of electrons pass through to an electromagnetic objective lens, which magnifies the image. The final image is seen as light and dark areas, referred to as a transmission electron micrograph. • Disadvantages: 1. requires a very thin slice of the specimen ...
Gram Staining - WordPress.com
... by their cell wall components. The procedure distinguishes between two groups : Gram Positive and Gram Negative by staining them red or violet. Gram Positive bacteria have a thicker layer of peptidoglycan in their cell wall, so they stain violet because the substance retains the crystal violet stain ...
... by their cell wall components. The procedure distinguishes between two groups : Gram Positive and Gram Negative by staining them red or violet. Gram Positive bacteria have a thicker layer of peptidoglycan in their cell wall, so they stain violet because the substance retains the crystal violet stain ...
MARKER GENE TECHNOLOGIES, Inc
... is colorless and nonfluorescent until hydrolyzed. This property is useful in diagnosing spontaneous hydrolysis during storage. Acetate groups, used on many fluorescent indicators, are analogous to AM ester groups and should be treated similarly. ...
... is colorless and nonfluorescent until hydrolyzed. This property is useful in diagnosing spontaneous hydrolysis during storage. Acetate groups, used on many fluorescent indicators, are analogous to AM ester groups and should be treated similarly. ...
Chapter 3
... unstained cells have little contrast with the surrounding medium. However, researchers do make discoveries about cell behavior looking at live ...
... unstained cells have little contrast with the surrounding medium. However, researchers do make discoveries about cell behavior looking at live ...
2 StainsInMicro
... particular carry negative charges and are responsible for giving the bacterial envelope an overall negative charge. Stains or dyes used to colorize cells in microbiology are usually ionic compounds that disassociate into ions when mixed with water. The ion of the compound responsible for the color i ...
... particular carry negative charges and are responsible for giving the bacterial envelope an overall negative charge. Stains or dyes used to colorize cells in microbiology are usually ionic compounds that disassociate into ions when mixed with water. The ion of the compound responsible for the color i ...
advanced non-invasive techniques in diagnosis of oral
... examination of cells obtained by their physical ...
... examination of cells obtained by their physical ...
Document
... Chromotrope Gram stain method In this method, the samples are stained in heated (50 degrees C to 55 degrees C) solutions of crystal violet and iodine used in Gram's stain, followed by a modified chromotrope solution (heated to 50 degrees C to 55 degrees C). The modified stain is composed of chromot ...
... Chromotrope Gram stain method In this method, the samples are stained in heated (50 degrees C to 55 degrees C) solutions of crystal violet and iodine used in Gram's stain, followed by a modified chromotrope solution (heated to 50 degrees C to 55 degrees C). The modified stain is composed of chromot ...
Staining Bacteria
... • Basic stains are cationic; when ionized, the chromogene exhibits a positive charge. Basic stains bind to negatively charged cell structures like nucleic acids. Methylene blue, crystal violet and carbolfuchsin are common basic stains. • Acidic stains are anionic; when ionized, the chromogen exhibi ...
... • Basic stains are cationic; when ionized, the chromogene exhibits a positive charge. Basic stains bind to negatively charged cell structures like nucleic acids. Methylene blue, crystal violet and carbolfuchsin are common basic stains. • Acidic stains are anionic; when ionized, the chromogen exhibi ...
Slide 1
... The crucial step in the staining process is the decolorizing step. The most accepted theory relies on the fact that the PPG is found in layers and the stain molecules are trapped within the many layers of the GP CW when they form the complex with the mordant Iodine ...
... The crucial step in the staining process is the decolorizing step. The most accepted theory relies on the fact that the PPG is found in layers and the stain molecules are trapped within the many layers of the GP CW when they form the complex with the mordant Iodine ...
Slide Master
... slides, with cover and inclinable to 45°. The two units may be used also like humid chambers and are furnished with a collector for discarded reagents. Each Slide Master has adjustable basis and check bead to maintain slides always in horizontal position. ...
... slides, with cover and inclinable to 45°. The two units may be used also like humid chambers and are furnished with a collector for discarded reagents. Each Slide Master has adjustable basis and check bead to maintain slides always in horizontal position. ...
Approach to blue stain fungi on ISPM 15-certified wood
... blue stain fungi species is difficult due to the time required to isolate and culture the fungi and the high-level of morphological taxonomic skills required to make the identification. Molecular-based approaches to species identification of active blue stain fungi exist (Khadempour et al. 2010; Roe ...
... blue stain fungi species is difficult due to the time required to isolate and culture the fungi and the high-level of morphological taxonomic skills required to make the identification. Molecular-based approaches to species identification of active blue stain fungi exist (Khadempour et al. 2010; Roe ...
Document
... action of an alcoholic solution. Those that resist decolorization by 95% ethanol are arbitrarily termed Gram positive and those that do not are Gram negative (the terms positive and negative have nothing to do with charges of the cell but based on differences in the cell wall structure of these two ...
... action of an alcoholic solution. Those that resist decolorization by 95% ethanol are arbitrarily termed Gram positive and those that do not are Gram negative (the terms positive and negative have nothing to do with charges of the cell but based on differences in the cell wall structure of these two ...
1. Types of Microscopy The Electromagnetic Spectrum 9/13/2016 Chapter 4A:
... Because, no matter how high the magnification or resolution, you need contrast to be able to see anything. If contrast is not sufficient in the sample or the microscopic method used, staining can provide the necessary contrast: • stains used for viewing bacteria via light microscopy are typically po ...
... Because, no matter how high the magnification or resolution, you need contrast to be able to see anything. If contrast is not sufficient in the sample or the microscopic method used, staining can provide the necessary contrast: • stains used for viewing bacteria via light microscopy are typically po ...
Light and Electron Microscopic Studies, Gene Mutation Analysis of
... glycosaminoglycans in Bowman’s histiocytes, keratocytes, between the stromal lamellae, Descemet’s membrane, and endothelium. These glycosaminoglycans stain positively with Alcian blue, Colloidal iron and periodic acid-Schiff (PAS). • Electron microscopy revealed that these deposits correspond to ele ...
... glycosaminoglycans in Bowman’s histiocytes, keratocytes, between the stromal lamellae, Descemet’s membrane, and endothelium. These glycosaminoglycans stain positively with Alcian blue, Colloidal iron and periodic acid-Schiff (PAS). • Electron microscopy revealed that these deposits correspond to ele ...
Staining of Blood parasites other than malaria parasites
... b. If microfilariae of Loa loa, follow steps iii, iv, v and vi because the sheath of Loa loa does not stain with Giemsa. For all other sheathed microfilariae, proceed only to step iv. since their sheaths stain with Giemsa.. c. Stain with a 1 in 10 dilution of Giemsa stain in pH 7.2 buffered water fo ...
... b. If microfilariae of Loa loa, follow steps iii, iv, v and vi because the sheath of Loa loa does not stain with Giemsa. For all other sheathed microfilariae, proceed only to step iv. since their sheaths stain with Giemsa.. c. Stain with a 1 in 10 dilution of Giemsa stain in pH 7.2 buffered water fo ...
Acid-fast stain
... fastness to the bacterium. Acid-fast bacteria, of which there are very few---the major genus Mycobacterium, have a high concentration of mycolic acid, a lipid, in their walls. Although difficult to stain, once the stain goes into the wall, the cell will not de-stain or decolorize easily. The ability ...
... fastness to the bacterium. Acid-fast bacteria, of which there are very few---the major genus Mycobacterium, have a high concentration of mycolic acid, a lipid, in their walls. Although difficult to stain, once the stain goes into the wall, the cell will not de-stain or decolorize easily. The ability ...
Managing tobacco blue mold in 2015: a
... tissue): azoxy products, Revus, Forum, Presidio • Actigard – a different kind of systemic fungicide ...
... tissue): azoxy products, Revus, Forum, Presidio • Actigard – a different kind of systemic fungicide ...
microscopy technique-2
... and 0.001 nm; thus, their resolving power is much greater, and they typically magnify objects 10,000× to 100,000×. EM – use beam of electron to illuminate and create magnified images of specimen ...
... and 0.001 nm; thus, their resolving power is much greater, and they typically magnify objects 10,000× to 100,000×. EM – use beam of electron to illuminate and create magnified images of specimen ...
Chapter 3 Lecture Notes
... 1. In a basic dye, the color is due to a cation (+). 2. In an acidic dye, the color is due to an anion (-). ii. Bacteria have a slight negative charge at pH 7, so basic dyes are attracted to and stain bacteria. 1. Crystal violet, methylene blue, malachite green, and safranin are all basic dyes. iii. ...
... 1. In a basic dye, the color is due to a cation (+). 2. In an acidic dye, the color is due to an anion (-). ii. Bacteria have a slight negative charge at pH 7, so basic dyes are attracted to and stain bacteria. 1. Crystal violet, methylene blue, malachite green, and safranin are all basic dyes. iii. ...
Boya
... • Methylene blue, Basic fuchsin • Provide the color contrast but impart the same color to all the organisms in a smear • Loffler's methylene blue: Sat. solution of M. blue in alcohol - 30mlKoH, 0.01% in water -100mlDissolve the dye in water, filter. For smear: stain for 3’. ...
... • Methylene blue, Basic fuchsin • Provide the color contrast but impart the same color to all the organisms in a smear • Loffler's methylene blue: Sat. solution of M. blue in alcohol - 30mlKoH, 0.01% in water -100mlDissolve the dye in water, filter. For smear: stain for 3’. ...
Alcian blue stain
Alcian blue or alcian blue (/ˈælʃən/) is any member of a family of polyvalent basic dyes, of which the Alcian blue 8G (also called Ingrain blue 1, and C.I. 74240, formerly called Alcian blue 8GX from the name of a batch of an ICI product) has been historically the most common and the most reliable member. It is used to stain acidic polysaccharides such as glycosaminoglycans in cartilages and other body structures, some types of mucopolysaccharides, sialylated glycocalyx of cells etc. For many of these targets it is one of the most widely used cationic dyes for both light and electron microscopy. Use of alcian blue has historically been a popular staining method in histology especially for light microscopy in paraffin embedded sections and in semithin resin sections. The tissue parts that specifically stain by this dye become blue to bluish-green after staining and are called ""Alcianophilic"" (akin to ""eosinophilic"" or ""sudanophilic""). Alcian blue staining can be combined with H&E staining, PAS staining and van Gieson staining methods. Alcian blue can be used to quantitate acidic glycans both in microspectrophotometric quantitation in solution or for staining glycoproteins in polyacrylamide gels or on western blots. Biochemists had used it to assay acid polysaccharides in urine since the 1960s for diagnosis of diseases like mucopolysaccharidosis but from 1970's, partly due to lack of availability of Alcian and partly due to length and tediousness of the procedure, alternative methods had to be developed e.g. Dimethyl methylene blue (DMB) method.Prof. J. E. Scott, the first person outside the dye Industry to crack the chemical secret of this dye comments:""Probably no other dyestuff has been applied to such wide variety of problems in biology and medicine. On the other hand, no other dyestuff had such a chequered history as AB.""In addition to its wide use as a stain Alcian blue has also been used in other diverse applications e.g. gelling agent for lubricating fluids, modifiers for electrodes, charged coating agents etc.