The PCR and -
... DNA ruler/ladder (1 kb) 130 µL of ready-diluted DNA ruler is provided in a 1.5 mL screw-capped tube. Before use, add 12 µL of bromophenol blue loading dye (use a microsyringe unit with a white graduated tip). Mix well by drawing the liquid up and down in the tip, then dispense 14 µL into each of 8 y ...
... DNA ruler/ladder (1 kb) 130 µL of ready-diluted DNA ruler is provided in a 1.5 mL screw-capped tube. Before use, add 12 µL of bromophenol blue loading dye (use a microsyringe unit with a white graduated tip). Mix well by drawing the liquid up and down in the tip, then dispense 14 µL into each of 8 y ...
An improved procedure for silver staining of protein bands on
... reported. The entire process, from gel to record, can be accomplished within three hours. Comparing with the classical Coomassie brilliant blue dying of proteins, the proposed silver staining procedure is faster, clearer, more sensitive and easily performed. It allows detecting protein bands in poly ...
... reported. The entire process, from gel to record, can be accomplished within three hours. Comparing with the classical Coomassie brilliant blue dying of proteins, the proposed silver staining procedure is faster, clearer, more sensitive and easily performed. It allows detecting protein bands in poly ...
77730 Gram Staining Kit - Sigma
... The cell walls for Gram-positive microorganisms have a higher peptidoglycan and lower lipid content than gramnegative bacteria. Bacteria cell walls are stained by the crystal violet. Iodine is subsequently added as a mordant to form the crystal violet-iodine complex so that the dye cannot be removed ...
... The cell walls for Gram-positive microorganisms have a higher peptidoglycan and lower lipid content than gramnegative bacteria. Bacteria cell walls are stained by the crystal violet. Iodine is subsequently added as a mordant to form the crystal violet-iodine complex so that the dye cannot be removed ...
STAINING
... affinity for the primary stain and resistance to decolorization by an acid alcohol solution. A variety of acid-fast staining procedures are employed, two of which are the ZiehlNeelsen (ZN) method and the Kinyoun (K) method. These differ primarily in that the ZN method uses heat as part of the staini ...
... affinity for the primary stain and resistance to decolorization by an acid alcohol solution. A variety of acid-fast staining procedures are employed, two of which are the ZiehlNeelsen (ZN) method and the Kinyoun (K) method. These differ primarily in that the ZN method uses heat as part of the staini ...
Jensara Study Guide
... 8. In week 1 you viewed Bacillus subtilis, Staphylococcus epidermidis, Nostoc, Saccharomyces cerevisiae, Spirillum volutans,, Amoeba proteus, Euglena and Paramecium. Know the description of these organisms (domain, shape, movement, special structures, etc.) Be able to recognize these organisms under ...
... 8. In week 1 you viewed Bacillus subtilis, Staphylococcus epidermidis, Nostoc, Saccharomyces cerevisiae, Spirillum volutans,, Amoeba proteus, Euglena and Paramecium. Know the description of these organisms (domain, shape, movement, special structures, etc.) Be able to recognize these organisms under ...
Identification of Unknown Bacteria Microbiology Laboratory Pre
... b. a type of enzyme that opens molecules c. the protein portion at the end of fimbriae that help bacteria cause infection d. a way to determine if a cell is in a hyper- or hypotonic environment e. a key for opening two chotomii 3. With which microscope objective magnification are you allowed to use ...
... b. a type of enzyme that opens molecules c. the protein portion at the end of fimbriae that help bacteria cause infection d. a way to determine if a cell is in a hyper- or hypotonic environment e. a key for opening two chotomii 3. With which microscope objective magnification are you allowed to use ...
Paraffin Histology
... Most methods contain instructions ‘dehydrate, clear and mount’. This involves steps 13 and 15 – 21 of the H&E staining method. Slides can be left in the final toluene before mounting without affecting the staining. Clearing refers to the action of these solutions to optically clear the sections maki ...
... Most methods contain instructions ‘dehydrate, clear and mount’. This involves steps 13 and 15 – 21 of the H&E staining method. Slides can be left in the final toluene before mounting without affecting the staining. Clearing refers to the action of these solutions to optically clear the sections maki ...
NUCLEAR AND CYTOPLASMIC STAINING
... nuclear envelope The double membrane that separates the nucleoplasm (see nucleus) of a cell from the cytoplasm. The membranes consist of lipid bilayers that are separated by a perinuclear space (or compart ...
... nuclear envelope The double membrane that separates the nucleoplasm (see nucleus) of a cell from the cytoplasm. The membranes consist of lipid bilayers that are separated by a perinuclear space (or compart ...
Amoeba Control Slides – Technical Memo
... guarantees reactivity of these control slides for one year from the date of receipt. Revalidate after one year to verify continued reactivity. Store at 15-30°C in a light deprived and humidity controlled environment. These positive control slides were produced from human surgical or autopsy tissues ...
... guarantees reactivity of these control slides for one year from the date of receipt. Revalidate after one year to verify continued reactivity. Store at 15-30°C in a light deprived and humidity controlled environment. These positive control slides were produced from human surgical or autopsy tissues ...
endospore stain
... Endospore production is a very important characteristic of some bacteria, allowing them to resist adverse environmental conditions such as desiccation, chemical exposure, extreme heat, etc. They were identified in the 1800s (John Tyndall developed a process for destroying them with intermittent heat ...
... Endospore production is a very important characteristic of some bacteria, allowing them to resist adverse environmental conditions such as desiccation, chemical exposure, extreme heat, etc. They were identified in the 1800s (John Tyndall developed a process for destroying them with intermittent heat ...
Acidic (Eosinophilic) and Basic Dyes
... structures ex. DNA, ribosomes, RNA – Euchromatin is DNA in USE. It is spread out, diffuse, and less stained. – Heterochromatin is condensed DNA, and stains dark blue. Lab #1 Slide #13 ...
... structures ex. DNA, ribosomes, RNA – Euchromatin is DNA in USE. It is spread out, diffuse, and less stained. – Heterochromatin is condensed DNA, and stains dark blue. Lab #1 Slide #13 ...
Gram cell staining
... Gram cell staining The most used stain in microbiology is the Gram stain developed in 1884 by Danish bacteriologist - Professor Hans Christian Joachim Gram. The Gram cell staining is one of physiological-biochemical method which can be useful in bacteria pathogen detection. Used Gram stain method, a ...
... Gram cell staining The most used stain in microbiology is the Gram stain developed in 1884 by Danish bacteriologist - Professor Hans Christian Joachim Gram. The Gram cell staining is one of physiological-biochemical method which can be useful in bacteria pathogen detection. Used Gram stain method, a ...
KROMATOGRAFI LAPIS TIPIS
... Add the vanillin to your container followed by ethanol to give a clear solu7on. Carefully add the sulfuric acid. The final product is a clear colourless solu7on. However, aWer some dipping of plates i ...
... Add the vanillin to your container followed by ethanol to give a clear solu7on. Carefully add the sulfuric acid. The final product is a clear colourless solu7on. However, aWer some dipping of plates i ...
Histopathology in Masson Trichrome stained muscle
... the muscle (e.g. from tendon to tendon) in dyW/dyW mice, it is sufficient to take pictures at one location within the muscle i.e. the middle. For consistency it is necessary to keep the location constant in all muscles and to mention which part of the muscle is analyzed. Some researchers perform his ...
... the muscle (e.g. from tendon to tendon) in dyW/dyW mice, it is sufficient to take pictures at one location within the muscle i.e. the middle. For consistency it is necessary to keep the location constant in all muscles and to mention which part of the muscle is analyzed. Some researchers perform his ...
Mycobacterium tuberculosis
... Cover the smear with acid-fast staining solution (Cabolfuchsin s in 5% Phenol ) Heat the slide gently for 10-15 min. (Keep the smear covered with staining solution while heating the slide and do not allow it to dry. Once you see that the staining solution that covers the smear is about to dry, add ...
... Cover the smear with acid-fast staining solution (Cabolfuchsin s in 5% Phenol ) Heat the slide gently for 10-15 min. (Keep the smear covered with staining solution while heating the slide and do not allow it to dry. Once you see that the staining solution that covers the smear is about to dry, add ...
The differential staining of Schizomycetes in tissue sections and in
... in which the rest of the dye is dissolved. The nucleus and fundamental tissue is stained only a light yellow (from the iodine) while the Schizomycetes, if any are present in the preparation, are an intense blue (often almost black). The intensity of the staining has not been equaled by any of the cu ...
... in which the rest of the dye is dissolved. The nucleus and fundamental tissue is stained only a light yellow (from the iodine) while the Schizomycetes, if any are present in the preparation, are an intense blue (often almost black). The intensity of the staining has not been equaled by any of the cu ...
Laboratory Exercise # 6: Gram Stain Purpose: The student will
... morphology (shape) and arrangement of bacteria. In order to classify bacteria into different groups a differential staining procedure must be done. A differential stain involves the use of two or more stains. Depending on the components of the bacterial cell wall or outer layers, the bacteria will e ...
... morphology (shape) and arrangement of bacteria. In order to classify bacteria into different groups a differential staining procedure must be done. A differential stain involves the use of two or more stains. Depending on the components of the bacterial cell wall or outer layers, the bacteria will e ...
Exercises 7/8/9/10/17 Endospore Stain: Exercise 7
... Acid fast microbes are pathogenic for humans, therefore observing them is critical. The diagnostic value of acid-fast stain is that pathogens retain color even in presence of acid. For example tuberculosis would retain the red dye carbolfuchsin even after it was washed with a powerful solvent made o ...
... Acid fast microbes are pathogenic for humans, therefore observing them is critical. The diagnostic value of acid-fast stain is that pathogens retain color even in presence of acid. For example tuberculosis would retain the red dye carbolfuchsin even after it was washed with a powerful solvent made o ...
Gram Stain
... • Developed by Hans Christian Gram in 1884 when he was studying bacteria from different respiratory diseases. ...
... • Developed by Hans Christian Gram in 1884 when he was studying bacteria from different respiratory diseases. ...
lab2-细菌的芽孢染色
... 二 实验原理 A few genera of bacteria, such as Bacillus and Clostridium have the ability to produce resistant survival forms termed endospores. Unlike the reproductive spores of fungi and plants, these endospores are resistant to heat, drying, radiation, and various chemical disinfectants. ...
... 二 实验原理 A few genera of bacteria, such as Bacillus and Clostridium have the ability to produce resistant survival forms termed endospores. Unlike the reproductive spores of fungi and plants, these endospores are resistant to heat, drying, radiation, and various chemical disinfectants. ...
2 common staining technique
... Staining is technique used in microscopy to enhance contrast in the microscopic image. Stains and dyes are frequently used in biological tissues for viewing, often with the aid of different microscopes. Stains may be used to define and examine bulk tissues (highlighting, for example, muscle fibers o ...
... Staining is technique used in microscopy to enhance contrast in the microscopic image. Stains and dyes are frequently used in biological tissues for viewing, often with the aid of different microscopes. Stains may be used to define and examine bulk tissues (highlighting, for example, muscle fibers o ...
Metode Mikrobiologis - Selamat Datang di Komunitas e
... Multiple staining reactions are employed Differentiate types of cells or cell’s structures base on their staining reactions, hence given different color The specimen must be “fixed” by heating or chemical ...
... Multiple staining reactions are employed Differentiate types of cells or cell’s structures base on their staining reactions, hence given different color The specimen must be “fixed” by heating or chemical ...
Vascular tissue microscopy - teacher notes
... Why are stains useful in microscopy? Why is Toluidine blue useful for this protocol? What did you find was the best way to modify the staining protocol to produce the most informative staining? What adjustments did you make to the microscope to optimise the image? What challenges were there in takin ...
... Why are stains useful in microscopy? Why is Toluidine blue useful for this protocol? What did you find was the best way to modify the staining protocol to produce the most informative staining? What adjustments did you make to the microscope to optimise the image? What challenges were there in takin ...
Lab # 3 Gram and Acid Fast stain
... crystal violet/Iodine from the gram – cells. The decolorizer, ethyl alcohol, is applied for 20 seconds. The gram + cells continue to appear purple while the others have become colorless. ...
... crystal violet/Iodine from the gram – cells. The decolorizer, ethyl alcohol, is applied for 20 seconds. The gram + cells continue to appear purple while the others have become colorless. ...
Alcian blue stain
Alcian blue or alcian blue (/ˈælʃən/) is any member of a family of polyvalent basic dyes, of which the Alcian blue 8G (also called Ingrain blue 1, and C.I. 74240, formerly called Alcian blue 8GX from the name of a batch of an ICI product) has been historically the most common and the most reliable member. It is used to stain acidic polysaccharides such as glycosaminoglycans in cartilages and other body structures, some types of mucopolysaccharides, sialylated glycocalyx of cells etc. For many of these targets it is one of the most widely used cationic dyes for both light and electron microscopy. Use of alcian blue has historically been a popular staining method in histology especially for light microscopy in paraffin embedded sections and in semithin resin sections. The tissue parts that specifically stain by this dye become blue to bluish-green after staining and are called ""Alcianophilic"" (akin to ""eosinophilic"" or ""sudanophilic""). Alcian blue staining can be combined with H&E staining, PAS staining and van Gieson staining methods. Alcian blue can be used to quantitate acidic glycans both in microspectrophotometric quantitation in solution or for staining glycoproteins in polyacrylamide gels or on western blots. Biochemists had used it to assay acid polysaccharides in urine since the 1960s for diagnosis of diseases like mucopolysaccharidosis but from 1970's, partly due to lack of availability of Alcian and partly due to length and tediousness of the procedure, alternative methods had to be developed e.g. Dimethyl methylene blue (DMB) method.Prof. J. E. Scott, the first person outside the dye Industry to crack the chemical secret of this dye comments:""Probably no other dyestuff has been applied to such wide variety of problems in biology and medicine. On the other hand, no other dyestuff had such a chequered history as AB.""In addition to its wide use as a stain Alcian blue has also been used in other diverse applications e.g. gelling agent for lubricating fluids, modifiers for electrodes, charged coating agents etc.