Methods of Microbiology
... • Works best in neutral or alkaline pH • Cell wall has slight negative charge at pH ...
... • Works best in neutral or alkaline pH • Cell wall has slight negative charge at pH ...
FORGED by God`s fingers in His furnace, Fate, My destiny drew near
... FORGED by God’s fingers in His furnace, Fate, My destiny drew near the glowing shore Where California hides her golden ore, Her rubies and her beryls ; gross and great, Her varied fruits and flowers alike create Glories most unimaginable, more Than Heaven’s own meadows match ; yet this is sore, A st ...
... FORGED by God’s fingers in His furnace, Fate, My destiny drew near the glowing shore Where California hides her golden ore, Her rubies and her beryls ; gross and great, Her varied fruits and flowers alike create Glories most unimaginable, more Than Heaven’s own meadows match ; yet this is sore, A st ...
Simple staining
... color is called the chromophore and the other part that helps in the coloring properties is called the auxochrome. The chromophore of a stain maybe charged –either positive charge or negative charge. A stain with a positively charged chromophore is called a cationic dye and one that has a negatively ...
... color is called the chromophore and the other part that helps in the coloring properties is called the auxochrome. The chromophore of a stain maybe charged –either positive charge or negative charge. A stain with a positively charged chromophore is called a cationic dye and one that has a negatively ...
Observation of bacteria using staining procedures Simple staining Gram staining
... – A drop of water is placed in the centre of a slide – One loopfuls of organisms is transferred to the centre of slide – Spread the organisms over the slide – The smear is allowed to dry – Slide is passed through flame several times to heat-kill and fix organisms • A bacterial stain is stained with ...
... – A drop of water is placed in the centre of a slide – One loopfuls of organisms is transferred to the centre of slide – Spread the organisms over the slide – The smear is allowed to dry – Slide is passed through flame several times to heat-kill and fix organisms • A bacterial stain is stained with ...
Chapter 3 Observing Microorganisms Through a Microscope
... • Gram positive cells have thick cell walls. They hold on to the primary stain. • Gram negative cells have thin cell wall. • One or two layers of peptidoglycan. They also have an outer membrane – lipids. • Alcohol causes damage to the lipids. Primary stain leaks out. ...
... • Gram positive cells have thick cell walls. They hold on to the primary stain. • Gram negative cells have thin cell wall. • One or two layers of peptidoglycan. They also have an outer membrane – lipids. • Alcohol causes damage to the lipids. Primary stain leaks out. ...
VIEW PDF - Retina Today
... a suitable secondary dye such as BBG could be used. However, a simpler option is to use a dual dye solution that contains trypan blue and BBG in a single injection to simultaneously stain the ERM and ILM. Surgeons may need to restain the ILM with the same dye following ERM removal. BBG is a good opt ...
... a suitable secondary dye such as BBG could be used. However, a simpler option is to use a dual dye solution that contains trypan blue and BBG in a single injection to simultaneously stain the ERM and ILM. Surgeons may need to restain the ILM with the same dye following ERM removal. BBG is a good opt ...
Chapter 4: Microscopy, Staining, and Classification
... -dyes used for staining are either acidic (negatively charged) or basic (positively charged) -staining occurs due to the bonding of the dyes to various parts of the cell -some stains do not create chemical bonds, but accumulate in certain parts of the cell. Simple Stains- ...
... -dyes used for staining are either acidic (negatively charged) or basic (positively charged) -staining occurs due to the bonding of the dyes to various parts of the cell -some stains do not create chemical bonds, but accumulate in certain parts of the cell. Simple Stains- ...
Cold Acid Fast Stain Technique
... 4. Remove the blotting paper with forceps. Gently rinse with water and drain. 5. Decolorize with acid-alcohol (not the Gram Stain decolorizer) using 1 “healthy” squirt, followed by a gentle water rinse. 6. Counterstain with methylene blue for 1 minute. 7. Rinse, blot dry with bibulous paper and obse ...
... 4. Remove the blotting paper with forceps. Gently rinse with water and drain. 5. Decolorize with acid-alcohol (not the Gram Stain decolorizer) using 1 “healthy” squirt, followed by a gentle water rinse. 6. Counterstain with methylene blue for 1 minute. 7. Rinse, blot dry with bibulous paper and obse ...
Acid
... Metachromatic staining principals • Stain aplied on fixed cell or tissue is of different color as is the result color • Ernst Babes inclusions – metachromatic granuls – volutine granuls – phosphate molecules - Corynebacterium diphteriae • Volutin – nucleoprotein complex – in cytoplasm of some bact ...
... Metachromatic staining principals • Stain aplied on fixed cell or tissue is of different color as is the result color • Ernst Babes inclusions – metachromatic granuls – volutine granuls – phosphate molecules - Corynebacterium diphteriae • Volutin – nucleoprotein complex – in cytoplasm of some bact ...
Disappearing Blue!
... MethyleneBlue Plus™ back to its blue form. When the dissolved oxygen has been consumed, the FlashBlue™ or Methylene Blue Plus™ is slowly reduced back to its colorless form by the remaining glucose, and the cycle can be repeated many times by further shaking. A white background helps to make the colo ...
... MethyleneBlue Plus™ back to its blue form. When the dissolved oxygen has been consumed, the FlashBlue™ or Methylene Blue Plus™ is slowly reduced back to its colorless form by the remaining glucose, and the cycle can be repeated many times by further shaking. A white background helps to make the colo ...
File
... Toluidine blue, methylene blue, hematoxylin, alcian blue Nucleic acids, some cytoplasmic components (rRNA and rER), glycosaminoglycans and acidic glycoproteins ...
... Toluidine blue, methylene blue, hematoxylin, alcian blue Nucleic acids, some cytoplasmic components (rRNA and rER), glycosaminoglycans and acidic glycoproteins ...
Alcian blue stain
Alcian blue or alcian blue (/ˈælʃən/) is any member of a family of polyvalent basic dyes, of which the Alcian blue 8G (also called Ingrain blue 1, and C.I. 74240, formerly called Alcian blue 8GX from the name of a batch of an ICI product) has been historically the most common and the most reliable member. It is used to stain acidic polysaccharides such as glycosaminoglycans in cartilages and other body structures, some types of mucopolysaccharides, sialylated glycocalyx of cells etc. For many of these targets it is one of the most widely used cationic dyes for both light and electron microscopy. Use of alcian blue has historically been a popular staining method in histology especially for light microscopy in paraffin embedded sections and in semithin resin sections. The tissue parts that specifically stain by this dye become blue to bluish-green after staining and are called ""Alcianophilic"" (akin to ""eosinophilic"" or ""sudanophilic""). Alcian blue staining can be combined with H&E staining, PAS staining and van Gieson staining methods. Alcian blue can be used to quantitate acidic glycans both in microspectrophotometric quantitation in solution or for staining glycoproteins in polyacrylamide gels or on western blots. Biochemists had used it to assay acid polysaccharides in urine since the 1960s for diagnosis of diseases like mucopolysaccharidosis but from 1970's, partly due to lack of availability of Alcian and partly due to length and tediousness of the procedure, alternative methods had to be developed e.g. Dimethyl methylene blue (DMB) method.Prof. J. E. Scott, the first person outside the dye Industry to crack the chemical secret of this dye comments:""Probably no other dyestuff has been applied to such wide variety of problems in biology and medicine. On the other hand, no other dyestuff had such a chequered history as AB.""In addition to its wide use as a stain Alcian blue has also been used in other diverse applications e.g. gelling agent for lubricating fluids, modifiers for electrodes, charged coating agents etc.