Production and Purification of Recombinant Fluorescent Protein
... unprotonated form that absorbs at approximately 475 nanometers. Regardless of the excitation wavelength, however, fluorescence emission has a maximum peak wavelength at 507 nanometers, although the peak is broad and not well defined. Denaturation of GFP requires treatment with 6 M guanidine hydrochl ...
... unprotonated form that absorbs at approximately 475 nanometers. Regardless of the excitation wavelength, however, fluorescence emission has a maximum peak wavelength at 507 nanometers, although the peak is broad and not well defined. Denaturation of GFP requires treatment with 6 M guanidine hydrochl ...
Intrinsic Disorder in Cell-signaling and Cancer
... reflects this connection. Over-expression or constitutive activation of some oncogenes may contribute to the loss of cell-cycle control observed in many tumors.16 A large number of protooncogenes (i.e. c-jun, c-fos, c-myc ) code for transcription factors required for cell-cycle progression and cell ...
... reflects this connection. Over-expression or constitutive activation of some oncogenes may contribute to the loss of cell-cycle control observed in many tumors.16 A large number of protooncogenes (i.e. c-jun, c-fos, c-myc ) code for transcription factors required for cell-cycle progression and cell ...
INPS: predicting the impact of non-synonymous variations on protein
... residue substitution, (ii) predict whether a residue substitution promotes a DDG increase or decrease (two class predictors) and (iii) predict whether a mutation is stabilizing, destabilizing or not affecting the protein stability (three class predictors). Noticeably, it is also very difficult to fi ...
... residue substitution, (ii) predict whether a residue substitution promotes a DDG increase or decrease (two class predictors) and (iii) predict whether a mutation is stabilizing, destabilizing or not affecting the protein stability (three class predictors). Noticeably, it is also very difficult to fi ...
Basic Peptide Bonds
... based on the type of structures commonly found in the given life form. This includes even the most basic organisms such as bacteria or fungi, though the peptides and polypeptides in these organisms do tend to react very differently than those in plants or animals. ...
... based on the type of structures commonly found in the given life form. This includes even the most basic organisms such as bacteria or fungi, though the peptides and polypeptides in these organisms do tend to react very differently than those in plants or animals. ...
Gene Section NOL3 (nucleolar protein 3 (apoptosis repressor with CARD domain))
... frames distal to the splice acceptor. Because of this frame shift, the C-terminus of the two encoded proteins differ as do their stop codons, each of which is in exon 4. One transcript is translated into ARC (Apoptosis Repressor with CARD (Caspase Recruitment Domain)) (Koseki et al., 1998). MYP is a ...
... frames distal to the splice acceptor. Because of this frame shift, the C-terminus of the two encoded proteins differ as do their stop codons, each of which is in exon 4. One transcript is translated into ARC (Apoptosis Repressor with CARD (Caspase Recruitment Domain)) (Koseki et al., 1998). MYP is a ...
Amino Acids, Peptides and Proteins
... For a dodecapeptide (20 aa) it requires 40 chemical steps (not counting special treatment for some side groups). If the yield is 90% per step the overall yield is only (0.940) 1.5% If the yield is 99% per step the overall yield is only (0.9940) 67% If the yield is 99.9% per step the overall yield is ...
... For a dodecapeptide (20 aa) it requires 40 chemical steps (not counting special treatment for some side groups). If the yield is 90% per step the overall yield is only (0.940) 1.5% If the yield is 99% per step the overall yield is only (0.9940) 67% If the yield is 99.9% per step the overall yield is ...
The Maize Abscisic Acid-Responsive Protein Rabl7 1s Located in
... retained in the wild-type peptide column but to a much lesser extent in the mutant peptide column. Together, these results indicated a specific interaction of the wild-type but not the mutant peptide to Rab17. ...
... retained in the wild-type peptide column but to a much lesser extent in the mutant peptide column. Together, these results indicated a specific interaction of the wild-type but not the mutant peptide to Rab17. ...
... The enthalpy, ∆Ho, is obtained from the slope of the denaturation curve, at TM. Circle the correct answer above. iv) (6 pts) Complete one of the following choices. Use the back of the preceding page if you need more space. Choice A: Based on the changes in enthalpy (∆Ho), what can you say about the ...
Replacing Traditional SDS-PAGE and Bradford Techniques
... This second level of data analysis, the so-called result-flagging software feature, provides a fast overview of the samples that were analyzed. Figure 6 shows an example of result flagging. The orange label reflects a sample containing a 37 kDa and a 34 kDa protein, and the yellow label reflects sa ...
... This second level of data analysis, the so-called result-flagging software feature, provides a fast overview of the samples that were analyzed. Figure 6 shows an example of result flagging. The orange label reflects a sample containing a 37 kDa and a 34 kDa protein, and the yellow label reflects sa ...
The Generic Nature of Protein Folding and Misfolding
... folding process (Matouschek et al., 1989; Fersht, 1999, 2000; Vendruscolo et al., 2001). The results of a wide range of studies suggest that the fundamental mechanism of folding can be described as “nucleation-condensation,” in which a folding nucleus of a small number of residues forms, about which ...
... folding process (Matouschek et al., 1989; Fersht, 1999, 2000; Vendruscolo et al., 2001). The results of a wide range of studies suggest that the fundamental mechanism of folding can be described as “nucleation-condensation,” in which a folding nucleus of a small number of residues forms, about which ...
Human Signaling Protein 14-3-3 Interacts With
... enzyme–digested PCR products into the yeast expression vectors pGBT9 and pGAD424 (Clontech Laboratories Inc, Palo Alto, CA). This resulted in the in-frame fusion of each cytoplasmic domain to the 38 end of either the GAL4 (1-147) DNA-binding domain (pGBT9) or the GAL4 (768-881) activation domain. Si ...
... enzyme–digested PCR products into the yeast expression vectors pGBT9 and pGAD424 (Clontech Laboratories Inc, Palo Alto, CA). This resulted in the in-frame fusion of each cytoplasmic domain to the 38 end of either the GAL4 (1-147) DNA-binding domain (pGBT9) or the GAL4 (768-881) activation domain. Si ...
In Depth Analysis of Protein Amino Acid Sequence and PTMs with
... • Peptides from the set of confident proteins are “modified” in-silico by trying all possible modifications in UNIMOD. • Speed up by de novo tags PeaksPTM: Mass spectrometry-based identification of peptides with unspecified modifications. Journal of Proteome Research 10.7 (2011) : 2930-2936 ...
... • Peptides from the set of confident proteins are “modified” in-silico by trying all possible modifications in UNIMOD. • Speed up by de novo tags PeaksPTM: Mass spectrometry-based identification of peptides with unspecified modifications. Journal of Proteome Research 10.7 (2011) : 2930-2936 ...
The Maize Abscisic Acid-Responsive Protein Rabl7
... retained in the wild-type peptide column but to a much lesser extent in the mutant peptide column. Together, these results indicated a specific interaction of the wild-type but not the mutant peptide to Rab17. ...
... retained in the wild-type peptide column but to a much lesser extent in the mutant peptide column. Together, these results indicated a specific interaction of the wild-type but not the mutant peptide to Rab17. ...
Application 1
... 3. Anti-proteinase 3: Autoantibody against the antigen proteinase-3, which is directly implicated in pathogenesis of the disease and is used to detect antigen bound to the array surface. 4. GaH-IgG-SWNT: The goat-anti-human antibody against the antiproteinase 3 antibody that is used for detection of ...
... 3. Anti-proteinase 3: Autoantibody against the antigen proteinase-3, which is directly implicated in pathogenesis of the disease and is used to detect antigen bound to the array surface. 4. GaH-IgG-SWNT: The goat-anti-human antibody against the antiproteinase 3 antibody that is used for detection of ...
Exploration of the Dynamic Properties of Protein Complexes
... search for structures that correlate with an activity state, and estimate the abundance of conformations in the living cell. The generator is based on a Markov Chain Monte Carlo simulation that uses the interaction dataset as input and is constrained by the physical resolution of the assay. We appli ...
... search for structures that correlate with an activity state, and estimate the abundance of conformations in the living cell. The generator is based on a Markov Chain Monte Carlo simulation that uses the interaction dataset as input and is constrained by the physical resolution of the assay. We appli ...
Clustering of Proteins
... currently used in practice employ evolutionary relationships between sequences to predict functional properties. Clustering of proteins is one such method for determining evolutionary relationships between proteins and thereby inferring functional properties. To perform a clustering on the proteins, ...
... currently used in practice employ evolutionary relationships between sequences to predict functional properties. Clustering of proteins is one such method for determining evolutionary relationships between proteins and thereby inferring functional properties. To perform a clustering on the proteins, ...
“Characterization of Proteins Interacting with Cystinosin” – Lay
... with cystinosin. This allowed us to identify proteins potentially important for cystinosin function, that were not necessarily expected from what was not known in the field of cystinosis. Indeed, we identified galectin-3, a protein known to be able to interact with sugar appended to certain proteins ...
... with cystinosin. This allowed us to identify proteins potentially important for cystinosin function, that were not necessarily expected from what was not known in the field of cystinosis. Indeed, we identified galectin-3, a protein known to be able to interact with sugar appended to certain proteins ...
Protein domain
A protein domain is a conserved part of a given protein sequence and (tertiary) structure that can evolve, function, and exist independently of the rest of the protein chain. Each domain forms a compact three-dimensional structure and often can be independently stable and folded. Many proteins consist of several structural domains. One domain may appear in a variety of different proteins. Molecular evolution uses domains as building blocks and these may be recombined in different arrangements to create proteins with different functions. Domains vary in length from between about 25 amino acids up to 500 amino acids in length. The shortest domains such as zinc fingers are stabilized by metal ions or disulfide bridges. Domains often form functional units, such as the calcium-binding EF hand domain of calmodulin. Because they are independently stable, domains can be ""swapped"" by genetic engineering between one protein and another to make chimeric proteins.