Seasonal regulation of a 24-kDa protein from red
... the present study, a calcium chloride extraction method was used to identify cell-wall-associated xylem proteins that accumulated during periods of cold acclimation. A 24-kDa protein represented the predominant protein in both total protein and CaCl2 extracts during cold acclimation of field-grown p ...
... the present study, a calcium chloride extraction method was used to identify cell-wall-associated xylem proteins that accumulated during periods of cold acclimation. A 24-kDa protein represented the predominant protein in both total protein and CaCl2 extracts during cold acclimation of field-grown p ...
QPrEST™—isotope-labeled multipeptide standards for
... Mass spectrometry (MS) enables absolute quantification of endogenous proteins by the use of isotopelabeled standards as internal references. QPrEST standards, currently available for >13,000 human proteins, represent a novel class of recombinantly produced heavy isotope–labeled standards that are ad ...
... Mass spectrometry (MS) enables absolute quantification of endogenous proteins by the use of isotopelabeled standards as internal references. QPrEST standards, currently available for >13,000 human proteins, represent a novel class of recombinantly produced heavy isotope–labeled standards that are ad ...
With-the-great-explosion-of-use-of
... structural diversity of proteins that begins with different amino acid sequences (primary structure) of polypeptide chains that fold into final conformation of secondary and tertiary structures. It was proposed that the information for folding to the native conformation is present in the amino acid ...
... structural diversity of proteins that begins with different amino acid sequences (primary structure) of polypeptide chains that fold into final conformation of secondary and tertiary structures. It was proposed that the information for folding to the native conformation is present in the amino acid ...
Hydrophobic Interaction Chromatography (HIC
... *Hydrophobic literally means “water fearing.” It refers to substances that do not mix well with water (i.e., Oil is hydrophobic). Note how it stays together in small beads and does not mix with water. Purpose: To separate hydrophobic proteins based on their tendency to stick to specially treated bea ...
... *Hydrophobic literally means “water fearing.” It refers to substances that do not mix well with water (i.e., Oil is hydrophobic). Note how it stays together in small beads and does not mix with water. Purpose: To separate hydrophobic proteins based on their tendency to stick to specially treated bea ...
10858_2016_75_MOESM1_ESM
... H-15N pairs. These are sequential 1HNi-1HNi+1 distance (2.8 Å), 1HNi-1H Ni+2 distance (4.2 Å), intra residue 1H Ni-2Hαi ...
... H-15N pairs. These are sequential 1HNi-1HNi+1 distance (2.8 Å), 1HNi-1H Ni+2 distance (4.2 Å), intra residue 1H Ni-2Hαi ...
Nonconvex Optimization Is Combinatorial Optimization
... folding is the process by which a protein, consisting of a long chain of amino acids, assumes its functional shape. The computational problem is to predict this final conformation from the known sequence of amino acids. This requires minimizing an energy function consisting mainly of a sum of pairwi ...
... folding is the process by which a protein, consisting of a long chain of amino acids, assumes its functional shape. The computational problem is to predict this final conformation from the known sequence of amino acids. This requires minimizing an energy function consisting mainly of a sum of pairwi ...
Severa1 Proteins lmported into Chloroplasts Form
... An abundant chloroplast stromal protein has been implicated in the assembly of the photosynthetic enzyme ribulose-1,.Ç-bisphosphate carboxylase/oxygenase (Rubisco) (Barraclough and Ellis, 1980; Roy et al., 1982; Cannon, Wang, and Roy, 1986; Gatenby et al., 1988), and has been referred to as either t ...
... An abundant chloroplast stromal protein has been implicated in the assembly of the photosynthetic enzyme ribulose-1,.Ç-bisphosphate carboxylase/oxygenase (Rubisco) (Barraclough and Ellis, 1980; Roy et al., 1982; Cannon, Wang, and Roy, 1986; Gatenby et al., 1988), and has been referred to as either t ...
appendix 1
... Oats contained in these products must have been specifically produced, prepared or processed to reduce their gluten content and this must not exceed 20mg / kg (20 ppm) These foods will be subject to future review ...
... Oats contained in these products must have been specifically produced, prepared or processed to reduce their gluten content and this must not exceed 20mg / kg (20 ppm) These foods will be subject to future review ...
8. ARTÍCULOS
... GTPases, and thus to regulate important cellular processes such as nucleocytoplasmic transport and mitotic spindle formation [4,5]. For this reason, it is thought that RLDs may act as guanine nucleotide exchange factors (GEFs) for small GTPases [6]. The HERC family can in turn be divided into two su ...
... GTPases, and thus to regulate important cellular processes such as nucleocytoplasmic transport and mitotic spindle formation [4,5]. For this reason, it is thought that RLDs may act as guanine nucleotide exchange factors (GEFs) for small GTPases [6]. The HERC family can in turn be divided into two su ...
Alfy, a novel FYVE-domain-containing protein associated with
... FYVE-domain-containing proteins with unknown functions, several have structures resembling endosomal FYVE proteins (Stenmark et al., 2002) and, in order to identify a possible FYVE domain protein with a non-endosomal function, we therefore sought a protein with an unknown function whose structure wa ...
... FYVE-domain-containing proteins with unknown functions, several have structures resembling endosomal FYVE proteins (Stenmark et al., 2002) and, in order to identify a possible FYVE domain protein with a non-endosomal function, we therefore sought a protein with an unknown function whose structure wa ...
Operon Models
... 5. Using a permanent pen, draw the shape of the active form of the repressor protein onto the lower portion of the noodle/operon, in the operator region. Make the shape simple since you will need to carve it out using a serrated knife. Also, carve a matching shape into the regulatory repressor prote ...
... 5. Using a permanent pen, draw the shape of the active form of the repressor protein onto the lower portion of the noodle/operon, in the operator region. Make the shape simple since you will need to carve it out using a serrated knife. Also, carve a matching shape into the regulatory repressor prote ...
Computational design of a pH-sensitive IgG binding
... efforts to engineer pH-dependent protein switches have either used structure-guided insertion of ionizable groups (8) or systematic histidine scanning mutagenesis (9, 10). These approaches often result in lower affinity interactions or low sensitivity to physiologically relevant pH changes. Computat ...
... efforts to engineer pH-dependent protein switches have either used structure-guided insertion of ionizable groups (8) or systematic histidine scanning mutagenesis (9, 10). These approaches often result in lower affinity interactions or low sensitivity to physiologically relevant pH changes. Computat ...
Selective Binding of the Scavenger Receptor C
... these key residues are present.2 Thus, SRCL differs from DCSIGN, the macrophage mannose receptor, serum mannosebinding protein, and other C-type lectins known to have roles in pathogen recognition, all of which fall into the second subgroup. Semiquantitative binding studies using cells expressing SR ...
... these key residues are present.2 Thus, SRCL differs from DCSIGN, the macrophage mannose receptor, serum mannosebinding protein, and other C-type lectins known to have roles in pathogen recognition, all of which fall into the second subgroup. Semiquantitative binding studies using cells expressing SR ...
Trichohyalin, an Intermediate Filament
... For these studies, the Mr 190,000 antiserum was purified by protein ASepharose CL-4B chromatography (17), and the IgG fraction was rechromatographed on an affinity column of the sheep follicle Mr 190,000 protein covalently linked to Sepharose 4B (Pharmacia). The purified antibody was dialyzed overni ...
... For these studies, the Mr 190,000 antiserum was purified by protein ASepharose CL-4B chromatography (17), and the IgG fraction was rechromatographed on an affinity column of the sheep follicle Mr 190,000 protein covalently linked to Sepharose 4B (Pharmacia). The purified antibody was dialyzed overni ...
Site-specific functionalization of proteins and their applications to
... around cells they perform a magnificently diverse set of functions. Besides providing structure and stability, proteins are involved in cell signaling, catalyzing reactions, storage and transport, and are therefore extensively studied. Over the years, tools have become available for researchers to r ...
... around cells they perform a magnificently diverse set of functions. Besides providing structure and stability, proteins are involved in cell signaling, catalyzing reactions, storage and transport, and are therefore extensively studied. Over the years, tools have become available for researchers to r ...
18_2014_1558_MOESM8_ESM
... represents protein relative abundance. Thresholds for decreased and increased abundance were set at <0.83 and >1.2, respectively. The P-value was calculated using Stouffer’s z-test, which enables P-values from several protein ratios belonging to the same analysis group to be combined, generating a s ...
... represents protein relative abundance. Thresholds for decreased and increased abundance were set at <0.83 and >1.2, respectively. The P-value was calculated using Stouffer’s z-test, which enables P-values from several protein ratios belonging to the same analysis group to be combined, generating a s ...
Review on Dengue viral Replication, assembly and entry into the
... serotypes 1–4). It is also a bifunctional enzyme with a methyltransferase domain (MTase; residues 1–296) at its N-terminal end and a RNA-dependent RNA polymerase (RdRp; residues 320–900) at its C-terminal end. The structure of the MTase domain was previously solved for DENV [50] and recently solved ...
... serotypes 1–4). It is also a bifunctional enzyme with a methyltransferase domain (MTase; residues 1–296) at its N-terminal end and a RNA-dependent RNA polymerase (RdRp; residues 320–900) at its C-terminal end. The structure of the MTase domain was previously solved for DENV [50] and recently solved ...
Protein domain
A protein domain is a conserved part of a given protein sequence and (tertiary) structure that can evolve, function, and exist independently of the rest of the protein chain. Each domain forms a compact three-dimensional structure and often can be independently stable and folded. Many proteins consist of several structural domains. One domain may appear in a variety of different proteins. Molecular evolution uses domains as building blocks and these may be recombined in different arrangements to create proteins with different functions. Domains vary in length from between about 25 amino acids up to 500 amino acids in length. The shortest domains such as zinc fingers are stabilized by metal ions or disulfide bridges. Domains often form functional units, such as the calcium-binding EF hand domain of calmodulin. Because they are independently stable, domains can be ""swapped"" by genetic engineering between one protein and another to make chimeric proteins.