Matrix assisted laser desorption/ionization
... (turnip sawfly) was monitored using MALDI-MSI. MS images of longitudinal cryo-sections of these larvae revealed the rapid sequestration and concentration of sinalbin in the haemolymph, rather than gut, as a strategy to detoxify ingested leaf ...
... (turnip sawfly) was monitored using MALDI-MSI. MS images of longitudinal cryo-sections of these larvae revealed the rapid sequestration and concentration of sinalbin in the haemolymph, rather than gut, as a strategy to detoxify ingested leaf ...
Toward the development of a Three-Dimensional Mid–Field Microscope
... slightly improves the spatial resolution of conventional microscopy3 (a factor of 2 ). Near field microscopy4 improves the resolution much further and allows an optical resolution of ~50 nm in the plane and ~5 nm along the optical axis (perpendicular to the sample surface). This is achieved by a se ...
... slightly improves the spatial resolution of conventional microscopy3 (a factor of 2 ). Near field microscopy4 improves the resolution much further and allows an optical resolution of ~50 nm in the plane and ~5 nm along the optical axis (perpendicular to the sample surface). This is achieved by a se ...
MeriameBerboucha
... Herriott cell used to produce optical time-delay so that pulses reach each other at the same time. Figure 5: Schematic of IGNIS, the OPCPA laser system that shall be used for the research and development phase before the construction of LCLS-II. Figure 1: Top left and right: laser enclosure for IGNI ...
... Herriott cell used to produce optical time-delay so that pulses reach each other at the same time. Figure 5: Schematic of IGNIS, the OPCPA laser system that shall be used for the research and development phase before the construction of LCLS-II. Figure 1: Top left and right: laser enclosure for IGNI ...
Part #1: Using Microscopes
... Microscopes 1. Carefully remove the dissecting microscope (two eyepieces) from under your lab table. Always keep the cover on the microscope when it is not in use. 2. What is the magnification of the lowest power?_________ Highest power?________ 3. How does the working distance affect the size of o ...
... Microscopes 1. Carefully remove the dissecting microscope (two eyepieces) from under your lab table. Always keep the cover on the microscope when it is not in use. 2. What is the magnification of the lowest power?_________ Highest power?________ 3. How does the working distance affect the size of o ...
Instrumental Analysis as Applied to Architectural Materials
... The light may be either reflected by the sample or transmitted through the sample. The image generated is magnified through lenses. Optical microscopy is used to analyze coatings, stones, and mortar and to identify constituents such as minerals (crystals). Microscopy is typically qualitative but may ...
... The light may be either reflected by the sample or transmitted through the sample. The image generated is magnified through lenses. Optical microscopy is used to analyze coatings, stones, and mortar and to identify constituents such as minerals (crystals). Microscopy is typically qualitative but may ...
Slide 1
... • Resist is partially reflective, so some light reaches resist bottom and is reflected. • Constructive and destructive interference between incident and reflected light results in a periodic intensity distribution across the resist thickness. • With change in exposure (light intensity) comes change ...
... • Resist is partially reflective, so some light reaches resist bottom and is reflected. • Constructive and destructive interference between incident and reflected light results in a periodic intensity distribution across the resist thickness. • With change in exposure (light intensity) comes change ...
Synchronized ti scattering microscopy
... Raman scattering (CARS) [1] or stimulated Raman scattering (SRS) [2], allows label-free imaging of biological samples with endogenous image contrast based on vibrational spectroscopy. Imaging with high spatial resolution and speed up to video-rate were demonstrated previously [3]. Recently, SRS has ...
... Raman scattering (CARS) [1] or stimulated Raman scattering (SRS) [2], allows label-free imaging of biological samples with endogenous image contrast based on vibrational spectroscopy. Imaging with high spatial resolution and speed up to video-rate were demonstrated previously [3]. Recently, SRS has ...
Evanescent-field optical microscopy: effects of polarization, tip
... dielectric probe tip and detecting the fraction that is converted to a homogeneous wave into the probe. The tip is scanned over the sample surface either in constant-height mode, resulting in an image presenting the optical signal variation, or in constant-optical-flux mode ("optical tunneling"), re ...
... dielectric probe tip and detecting the fraction that is converted to a homogeneous wave into the probe. The tip is scanned over the sample surface either in constant-height mode, resulting in an image presenting the optical signal variation, or in constant-optical-flux mode ("optical tunneling"), re ...
Fiber Optic
... Their application is in long distance, high data rate requirements. Lasers are common in single mode optical fiber applications and their light power covers a very narrow spectrum, usually less than 3 nm. This results in a low chromatic dispersion value and hence high fiber bandwidth. Their life spa ...
... Their application is in long distance, high data rate requirements. Lasers are common in single mode optical fiber applications and their light power covers a very narrow spectrum, usually less than 3 nm. This results in a low chromatic dispersion value and hence high fiber bandwidth. Their life spa ...
STATE UNIVERSITY OF NEW YORK COLLEGE OF TECHNOLOGY CANTON, NEW YORK
... This course explores the production and nature of light including: the laws of reflection and refraction, theory of image formation, principles of wave optics (including interference, diffraction and polarization), fundamentals of fiber optic theory, principles of lasers and laser safety, and the ba ...
... This course explores the production and nature of light including: the laws of reflection and refraction, theory of image formation, principles of wave optics (including interference, diffraction and polarization), fundamentals of fiber optic theory, principles of lasers and laser safety, and the ba ...
LABORATORY TECHNIQUES
... that it is as close to the laser beam axis as possible. This will reduce aberrations, provide optimum light economy, and ease alignment of the pinhole. 2. Use the z-axis adjustment micrometer to move the MO as far away as possible from the x and y micrometer spindles. 3. Carefully remove the PM from ...
... that it is as close to the laser beam axis as possible. This will reduce aberrations, provide optimum light economy, and ease alignment of the pinhole. 2. Use the z-axis adjustment micrometer to move the MO as far away as possible from the x and y micrometer spindles. 3. Carefully remove the PM from ...
Introduction to Optical Engineering and Design ENSC 376
... optics, and less on the physics behind the behaviour. It starts with a basic explanation of the concepts of light, as electromagnetic radiation. Then it looks how light is generated, at both the atomic and black body level. Next optical interaction with materials is discussed beginning with reflecti ...
... optics, and less on the physics behind the behaviour. It starts with a basic explanation of the concepts of light, as electromagnetic radiation. Then it looks how light is generated, at both the atomic and black body level. Next optical interaction with materials is discussed beginning with reflecti ...
Fast STED microscopy
... filtered with single-mode polarization-maintaining fibers (PM460HP, Thorlabs; PMC-6304.6-NA011-3-APC-200-P with a cutoff at 570 nm, Schäfter + Kirchhoff, Germany). The two STED beams passed through two polymer masks imprinting 0 to 2π helical phase-ramps (VPP-A1, RPC Photonics, USA) in order to obta ...
... filtered with single-mode polarization-maintaining fibers (PM460HP, Thorlabs; PMC-6304.6-NA011-3-APC-200-P with a cutoff at 570 nm, Schäfter + Kirchhoff, Germany). The two STED beams passed through two polymer masks imprinting 0 to 2π helical phase-ramps (VPP-A1, RPC Photonics, USA) in order to obta ...
Fast STED microscopy
... filtered with single-mode polarization-maintaining fibers (PM460HP, Thorlabs; PMC-6304.6-NA011-3-APC-200-P with a cutoff at 570 nm, Schäfter + Kirchhoff, Germany). The two STED beams passed through two polymer masks imprinting 0 to 2π helical phase-ramps (VPP-A1, RPC Photonics, USA) in order to obta ...
... filtered with single-mode polarization-maintaining fibers (PM460HP, Thorlabs; PMC-6304.6-NA011-3-APC-200-P with a cutoff at 570 nm, Schäfter + Kirchhoff, Germany). The two STED beams passed through two polymer masks imprinting 0 to 2π helical phase-ramps (VPP-A1, RPC Photonics, USA) in order to obta ...
Superior Pointing Stability Simplifies MPE Imaging
... in the case of the Coherent Chameleon series. For instance, the long wavelength is particularly useful for optimum excitation of red fluorophores such as mcherry. And more recently, the integration of smoothly and widely variable group velocity dispersion (GVD) compensation of up to 47,000 fs2 – ena ...
... in the case of the Coherent Chameleon series. For instance, the long wavelength is particularly useful for optimum excitation of red fluorophores such as mcherry. And more recently, the integration of smoothly and widely variable group velocity dispersion (GVD) compensation of up to 47,000 fs2 – ena ...
Transmission Electron Microscopy -TEM
... liquid nitrogen or liquid helium temperatures. This allows imaging specimens prepared in vitreous ice, the preferred preparation technique for imaging individual molecules or macromolecular assemblies. • In analytical TEMs the elemental composition of the specimen can be determined by analysing its ...
... liquid nitrogen or liquid helium temperatures. This allows imaging specimens prepared in vitreous ice, the preferred preparation technique for imaging individual molecules or macromolecular assemblies. • In analytical TEMs the elemental composition of the specimen can be determined by analysing its ...
Phase contrast microscopy (PCM) represents a major breakthrough
... image, i.e. DC component, before the light is detected. For coherent illumination, this high-pass operation can be easily accomplished by placing an obstruction on-axis at the Fourier plane of the objective (see Fig. 7). Note that, in the absence of the specimen, the incident plane wave is focused o ...
... image, i.e. DC component, before the light is detected. For coherent illumination, this high-pass operation can be easily accomplished by placing an obstruction on-axis at the Fourier plane of the objective (see Fig. 7). Note that, in the absence of the specimen, the incident plane wave is focused o ...
Parallelized STED fluorescence nanoscopy
... Since the beginning of the 20th century, far-field optical microscopy has been essentially limited to >λ/2 > 200 nm in resolution, with λ denoting the wavelength of light. While electron and scanning probe microscopy have provided much finer details since then, they lack the possibility of non-invas ...
... Since the beginning of the 20th century, far-field optical microscopy has been essentially limited to >λ/2 > 200 nm in resolution, with λ denoting the wavelength of light. While electron and scanning probe microscopy have provided much finer details since then, they lack the possibility of non-invas ...
How I discovered phase contrast
... passing the strips, in the second case by the unequal light paths, i.e. by the unequal phases. I therefore distinguish the two by calling the first kind an amplitude grating, the second a phase grating. Or in the general case of an irregular structure, an « amplitude object », resp. a « phase object ...
... passing the strips, in the second case by the unequal light paths, i.e. by the unequal phases. I therefore distinguish the two by calling the first kind an amplitude grating, the second a phase grating. Or in the general case of an irregular structure, an « amplitude object », resp. a « phase object ...
ME 557 Howmwork #1
... 3. Fiber coupling spheres. Tiny glass balls are often used as lenses to couple light into and out of optical fibers. The fiber end is located at a distance f from the sphere. For a sphere of radius a=1 mm and refractive index n =1.8, determine f such that a ray parallel to the optical axis at a dist ...
... 3. Fiber coupling spheres. Tiny glass balls are often used as lenses to couple light into and out of optical fibers. The fiber end is located at a distance f from the sphere. For a sphere of radius a=1 mm and refractive index n =1.8, determine f such that a ray parallel to the optical axis at a dist ...
Analysis of plant tissue images obtained by confocal tandem
... of the cells. In Fig. 5 the exact cell area distribution is presented. It is seen that the distribution obtained by the reference method is moved to the left. However, there are no significant differences in the range around the peaks. The biggest differences appear on the tails of the distributions ...
... of the cells. In Fig. 5 the exact cell area distribution is presented. It is seen that the distribution obtained by the reference method is moved to the left. However, there are no significant differences in the range around the peaks. The biggest differences appear on the tails of the distributions ...
Supplementary Figure 1 - Word file (395 KB )
... procedures. GFP-Yap1 plasmids were transformed into an isogenic yap1::KanMX strain derived from the S. cerevisiae YPH499 parental strain. ...
... procedures. GFP-Yap1 plasmids were transformed into an isogenic yap1::KanMX strain derived from the S. cerevisiae YPH499 parental strain. ...
Microscope Objective
... photography using white light, most apochromats suffer some curvature of field but this is corrected for in plan apochromats. This large amount of color correction makes plan apochromats ideal for fluorescent imaging. This design also supports large numerical apertures (NA). The NA is the square roo ...
... photography using white light, most apochromats suffer some curvature of field but this is corrected for in plan apochromats. This large amount of color correction makes plan apochromats ideal for fluorescent imaging. This design also supports large numerical apertures (NA). The NA is the square roo ...
Experimental demonstration of a light-ray-direction
... is, imaging in which depth appears inverted). This has been realized experimentally [17,18]. Different cases of two confocal lenslet arrays with the same focal length but aligned such that corresponding lenslets in the two arrays do not share the same optical axis, or in which the alignment varies a ...
... is, imaging in which depth appears inverted). This has been realized experimentally [17,18]. Different cases of two confocal lenslet arrays with the same focal length but aligned such that corresponding lenslets in the two arrays do not share the same optical axis, or in which the alignment varies a ...
Confocal microscopy
Confocal microscopy is an optical imaging technique for increasing optical resolution and contrast of a micrograph by means of adding a spatial pinhole placed at the confocal plane of the lens to eliminate out-of-focus light. It enables the reconstruction of three-dimensional structures from the obtained images. This technique has gained popularity in the scientific and industrial communities and typical applications are in life sciences, semiconductor inspection and materials science.