Lect03_Bi177_MicroscopeOptics
... Collapse of Newton's corpuscular theory and the rise of the wave theory • By the 1800’s the wave theory was required to explain such phenomenon as diffraction, interference and refraction. • Airy disk is an intensity distribution of a diffraction limited spot helpful for defining resolution. ...
... Collapse of Newton's corpuscular theory and the rise of the wave theory • By the 1800’s the wave theory was required to explain such phenomenon as diffraction, interference and refraction. • Airy disk is an intensity distribution of a diffraction limited spot helpful for defining resolution. ...
Abstract
... manipulate a nucleus by (VUV) laser light for the first time. Apart from exciting fundamental research questions (including the search for temporal variations of fundamental constants), this well-shielded narrow-linewidth transition opens up the possibility to realize a compact solid-state optical t ...
... manipulate a nucleus by (VUV) laser light for the first time. Apart from exciting fundamental research questions (including the search for temporal variations of fundamental constants), this well-shielded narrow-linewidth transition opens up the possibility to realize a compact solid-state optical t ...
Integration of a semiconductor laser source on silicon
... plane so that it can be measured. Up to date, the focus of research has been on designs having a vertical current injection (VCI) scheme, meaning that the contacts are situated above and below the gain material. The novelty of our laser design concept is to instead use a lateral current injection (L ...
... plane so that it can be measured. Up to date, the focus of research has been on designs having a vertical current injection (VCI) scheme, meaning that the contacts are situated above and below the gain material. The novelty of our laser design concept is to instead use a lateral current injection (L ...
Institute of Optical Science and Technology (IOSAT) The Chinese
... trapping is the measurement of forces (on the order of pico-Newton) between biological objects such as DNA, RNA, protein, cell, and tissues. For such applications, optical forces have to be measured and calibrated. To date the most popular method for the measurement of the optical transverse gradien ...
... trapping is the measurement of forces (on the order of pico-Newton) between biological objects such as DNA, RNA, protein, cell, and tissues. For such applications, optical forces have to be measured and calibrated. To date the most popular method for the measurement of the optical transverse gradien ...
Nanometer optical coherence tomography using
... [7] Jasny et al.: A single-shot spectrograph for the soft X-ray region, Review of Scientific Instruments, 1994 [8] Fuchs et al., Sensitivity calibration of an imaging extreme ultraviolet spectrometer-detector system for determining the efficiency of broadband extreme ultraviolet sources, Rev. Sci. I ...
... [7] Jasny et al.: A single-shot spectrograph for the soft X-ray region, Review of Scientific Instruments, 1994 [8] Fuchs et al., Sensitivity calibration of an imaging extreme ultraviolet spectrometer-detector system for determining the efficiency of broadband extreme ultraviolet sources, Rev. Sci. I ...
1 I. The Unobservable-Observable Distinction (UOD) A. UOD`s
... a manner roughly analogous to that by which we now see by means of photons.” D. Van Fraassen on the UOD: 1. In his own words: “The human organism is, from the point of view of physics, a certain kind of measuring apparatus. As such it has certain limitations—which will be described in detail in the ...
... a manner roughly analogous to that by which we now see by means of photons.” D. Van Fraassen on the UOD: 1. In his own words: “The human organism is, from the point of view of physics, a certain kind of measuring apparatus. As such it has certain limitations—which will be described in detail in the ...
The Fabry-Perot Cavity
... Derivation of the Laser Threshold Condition In the steady-state, the light (plane wave assumption) should remain unchanged after one round trip (2L). In other words, the gain = loss at threshold. An energy pumped in above threshold is converted into photons. R1 and R2 are the power reflectivities o ...
... Derivation of the Laser Threshold Condition In the steady-state, the light (plane wave assumption) should remain unchanged after one round trip (2L). In other words, the gain = loss at threshold. An energy pumped in above threshold is converted into photons. R1 and R2 are the power reflectivities o ...
... optical CT scanning for gel dosimetry Optical CT scanning is a convenient, bench-top method of imaging 3D dose distributions in gel dosimeters such as BANG®. These scanners offer medical physicists a powerful tool for treatment plan validation in IMRT and conformal RTP. However, one of the limitatio ...
Overview - FOSSweb
... Students live in a world where creating infinitesimal objects and investigating small worlds is an integral part of developing technologies. Contemporary microscopes have developed to the point that they can be used to help manipulate objects as small as individual atoms. Microscopic electronic comp ...
... Students live in a world where creating infinitesimal objects and investigating small worlds is an integral part of developing technologies. Contemporary microscopes have developed to the point that they can be used to help manipulate objects as small as individual atoms. Microscopic electronic comp ...
Theoretical modeling and evaluation of the axial resolution of the
... Confocal imaging systems are attractive because of their enhanced three-dimensional imaging capability.1 The optical system images light scattered from the regions in the neighborhood of the focal plane and this is focused onto a pinhole. The confocal pinhole selectively allows light from a specific ...
... Confocal imaging systems are attractive because of their enhanced three-dimensional imaging capability.1 The optical system images light scattered from the regions in the neighborhood of the focal plane and this is focused onto a pinhole. The confocal pinhole selectively allows light from a specific ...
The Electron Microscope as an Illustration of the Wave Nature of the
... has a wavelength of 10-12 metres, while the light wave has a value of 5 x 10-7 metres, at first sight it would seem that the resolving power of the electron microscope should be nearly a million times greater. However, this advantage is not as great as this, because electron lenses are not as free o ...
... has a wavelength of 10-12 metres, while the light wave has a value of 5 x 10-7 metres, at first sight it would seem that the resolving power of the electron microscope should be nearly a million times greater. However, this advantage is not as great as this, because electron lenses are not as free o ...
Electron Microscopy and Autoradiography
... SEM images are usually easy to interpret because they present a 3D view that appears to be illuminated from above, in the same way that large objects are seen with highlights and shadows caused by light from above. Autoradiography Autoradiography is a method for localizing newly synthesized macromol ...
... SEM images are usually easy to interpret because they present a 3D view that appears to be illuminated from above, in the same way that large objects are seen with highlights and shadows caused by light from above. Autoradiography Autoradiography is a method for localizing newly synthesized macromol ...
Optical trapping and surgery of living yeast cells using a single laser
... ABSTRACT: Near-infrared (NIR) lasers have recently found numerous applications in biomedical research due to their non-destructive or localized effects on cells and tissues. Using a continuous-wave (CW) NIR laser for optical trapping has been demonstrated as a contact-free and non-invasive tool for ...
... ABSTRACT: Near-infrared (NIR) lasers have recently found numerous applications in biomedical research due to their non-destructive or localized effects on cells and tissues. Using a continuous-wave (CW) NIR laser for optical trapping has been demonstrated as a contact-free and non-invasive tool for ...
as a PDF - Department of Engineering Science
... magnification different from Eq. (1) cannot be a perfect imaging system. Such is the case in microscopy, for instance, where systems are designed to grossly magnify the object under investigation and consequently there is a breakdown of perfect three-dimensional imaging. For cases where the object i ...
... magnification different from Eq. (1) cannot be a perfect imaging system. Such is the case in microscopy, for instance, where systems are designed to grossly magnify the object under investigation and consequently there is a breakdown of perfect three-dimensional imaging. For cases where the object i ...
Supplementary Methods, Figure, Table and Movie Legends
... Farn2Palm was achieved using 5 % 405 nm laser power, 4 µs/pixel dwell time, and a 1 frame activation pulse. Images were captured every 5 s for 75 frames. At least three animals were imaged for each probe, and care was taken to minimize pixel saturation in the raw data. The level of photo-bleaching w ...
... Farn2Palm was achieved using 5 % 405 nm laser power, 4 µs/pixel dwell time, and a 1 frame activation pulse. Images were captured every 5 s for 75 frames. At least three animals were imaged for each probe, and care was taken to minimize pixel saturation in the raw data. The level of photo-bleaching w ...
here - TCD Maths home - Trinity College Dublin
... by Köhler and Rohr, allowed for an increase in resolving power of about a factor of two, but required more expensive quartz optical components. At this point it was believed that obtaining an image with sub-micrometer information was simply impossible due to this wavelength constraint. In 1891 it wa ...
... by Köhler and Rohr, allowed for an increase in resolving power of about a factor of two, but required more expensive quartz optical components. At this point it was believed that obtaining an image with sub-micrometer information was simply impossible due to this wavelength constraint. In 1891 it wa ...
“Pixel” team
... We are now willing to install a new instrument combining improved versatility, multiple beam alignment capability and stability. This instrument is based on two objectives instead of three in the previous versions. The change in design from three to two objectives necessitates the simultaneous coupl ...
... We are now willing to install a new instrument combining improved versatility, multiple beam alignment capability and stability. This instrument is based on two objectives instead of three in the previous versions. The change in design from three to two objectives necessitates the simultaneous coupl ...
LAB 1 - SIMPLE DIFFRACTION, FOURIER OPTICS AND ACOUSTO
... focus, any spatial variations in the beam (dashed lines in figure) will not pass through the pinhole. (Note that when the objective is highly defocused, the wave fronts entering the pinhole are closer to planar than to spherical. We will use this in several sections.) The filtered light leaving the ...
... focus, any spatial variations in the beam (dashed lines in figure) will not pass through the pinhole. (Note that when the objective is highly defocused, the wave fronts entering the pinhole are closer to planar than to spherical. We will use this in several sections.) The filtered light leaving the ...
Ray Box Lab - Iona Physics
... To locate the focus and measure the focal length several optical components. ...
... To locate the focus and measure the focal length several optical components. ...
Adaptive Optics and the cone mosaic
... collimated and then reflected off of a beam-splitting mirror. As in confocal SLO, light must pass through both a horizontal and a vertical scanning mirror before and after the eye is scanned to align the moving beam for eventual retinal faster images of the retina. Additionally, the light is reflecte ...
... collimated and then reflected off of a beam-splitting mirror. As in confocal SLO, light must pass through both a horizontal and a vertical scanning mirror before and after the eye is scanned to align the moving beam for eventual retinal faster images of the retina. Additionally, the light is reflecte ...
The Care and Feeding of the Microscope Lab 5
... Sizes are shown on a metric scale; names in red are organisms studied in microbiology. Chlamydia and Rickettsia are groups of bacteria that are much smaller in size than other bacteria. The range of effective use for various instruments is also depicted. E ve l yn I. Mi l i a n - In s tru c to r ...
... Sizes are shown on a metric scale; names in red are organisms studied in microbiology. Chlamydia and Rickettsia are groups of bacteria that are much smaller in size than other bacteria. The range of effective use for various instruments is also depicted. E ve l yn I. Mi l i a n - In s tru c to r ...
Document
... If field diameter at 40X is measured to be 5.8 mm ,then the diameter at 400X must be 0.58mm 5.8 x 40X = ? x 400X HPD = 5.8 x 40X 400X ...
... If field diameter at 40X is measured to be 5.8 mm ,then the diameter at 400X must be 0.58mm 5.8 x 40X = ? x 400X HPD = 5.8 x 40X 400X ...
Orbital Dynamics of the Laser Interferometer Space Antenna
... any higher order diffractions and insure a clean Gaussian beam. The light is then reflected off a mirror (6) and sent through a λ/2 wave plate (7) and onto the SLM (8). The λ/2 wave plate has two purposes. The first purpose is to allow the user to control the orientation of the plane polarized light ...
... any higher order diffractions and insure a clean Gaussian beam. The light is then reflected off a mirror (6) and sent through a λ/2 wave plate (7) and onto the SLM (8). The λ/2 wave plate has two purposes. The first purpose is to allow the user to control the orientation of the plane polarized light ...
DG Papazoglou et al.
... are lead to a better understanding of the physical process. Our results clearly support a photochemical mechanism for low repetition rate laser sources! D.G. Papazoglou et al., Opt. Lett. 31, 1441 (2006) , D. G. Papazoglou et al., Opt. Lett., 32, 2055 (2007), D.G. Papazoglou et al., (invited) to app ...
... are lead to a better understanding of the physical process. Our results clearly support a photochemical mechanism for low repetition rate laser sources! D.G. Papazoglou et al., Opt. Lett. 31, 1441 (2006) , D. G. Papazoglou et al., Opt. Lett., 32, 2055 (2007), D.G. Papazoglou et al., (invited) to app ...
Chapter 3: Concepts and Tools for Studying Microorganisms
... • Mycobacteria can be stained with carbol-fuchsin in the acid-fast technique Light Microscopy Has Other Optical Configurations • Phase-contrast microscopy a special condenser and objective lenses to allow observers to view living, unstained organisms • Dark-field microscopy shows the specimen agai ...
... • Mycobacteria can be stained with carbol-fuchsin in the acid-fast technique Light Microscopy Has Other Optical Configurations • Phase-contrast microscopy a special condenser and objective lenses to allow observers to view living, unstained organisms • Dark-field microscopy shows the specimen agai ...
Confocal microscopy
Confocal microscopy is an optical imaging technique for increasing optical resolution and contrast of a micrograph by means of adding a spatial pinhole placed at the confocal plane of the lens to eliminate out-of-focus light. It enables the reconstruction of three-dimensional structures from the obtained images. This technique has gained popularity in the scientific and industrial communities and typical applications are in life sciences, semiconductor inspection and materials science.