super-resolved fluorescence microscopy
... methods. 2.1 Far-field. Resolution improvements have been made with confocal (Cremer and Cremer, 1978; Sheppard and Wilson, 1981; Brakenhoff et al., 1985)) and multiphoton microscopy (Zipfel et al., 2003; Hell and Stelzer, 2002). Both these methods allow for effective background suppression and have ...
... methods. 2.1 Far-field. Resolution improvements have been made with confocal (Cremer and Cremer, 1978; Sheppard and Wilson, 1981; Brakenhoff et al., 1985)) and multiphoton microscopy (Zipfel et al., 2003; Hell and Stelzer, 2002). Both these methods allow for effective background suppression and have ...
S U P E R -R E S O LV... Scientific Background on the Nobel Prize in Chemistry 2014
... methods. 2.1 Far-field. Resolution improvements have been made with confocal (Cremer and Cremer, 1978; Sheppard and Wilson, 1981; Brakenhoff et al., 1985)) and multiphoton microscopy (Zipfel et al., 2003; Hell and Stelzer, 2002). Both these methods allow for effective background suppression and have ...
... methods. 2.1 Far-field. Resolution improvements have been made with confocal (Cremer and Cremer, 1978; Sheppard and Wilson, 1981; Brakenhoff et al., 1985)) and multiphoton microscopy (Zipfel et al., 2003; Hell and Stelzer, 2002). Both these methods allow for effective background suppression and have ...
Microscopes - Photonics Research Group
... eyepiece, albeit with residual error of the secondary spectrum and appreciable curvature of field. Early high-resolution compound microscopes were equipped with an (oilimmersible) Abbe condenser whose iris diaphragm, placed at the front focal plane of the condenser, could be off-centered to achieve ...
... eyepiece, albeit with residual error of the secondary spectrum and appreciable curvature of field. Early high-resolution compound microscopes were equipped with an (oilimmersible) Abbe condenser whose iris diaphragm, placed at the front focal plane of the condenser, could be off-centered to achieve ...
PowerPoint file
... • Bacteria: 1 - 5 µm (some much longer than wide) • Rickettsia: 0.4 µm = _________ nm • Chlamydia and Mycoplasma: 0.25 µm • Viruses: 20 – 250 nm ...
... • Bacteria: 1 - 5 µm (some much longer than wide) • Rickettsia: 0.4 µm = _________ nm • Chlamydia and Mycoplasma: 0.25 µm • Viruses: 20 – 250 nm ...
Resolution scaling in STED microscopy
... dark and bright states, to achieve such resolutions have since been demonstrated [8-13]. At the same time, STED has been applied to a multitude of imaging tasks [14-17] mainly in biology and more recently, STED microscopes have become commercially available. The resolution achieved by such a far-fie ...
... dark and bright states, to achieve such resolutions have since been demonstrated [8-13]. At the same time, STED has been applied to a multitude of imaging tasks [14-17] mainly in biology and more recently, STED microscopes have become commercially available. The resolution achieved by such a far-fie ...
AOTF-based system for image cytometry
... interferometry (FTI), pushbroom imagers (2-dimensional imaging spectrometers)7,8, and whiskbroom (raster-scanning) multispectral devices – usually part of confocal instruments9. Although these technologies have been successfully integrated with commercially available instruments (Nuance from CRI, PA ...
... interferometry (FTI), pushbroom imagers (2-dimensional imaging spectrometers)7,8, and whiskbroom (raster-scanning) multispectral devices – usually part of confocal instruments9. Although these technologies have been successfully integrated with commercially available instruments (Nuance from CRI, PA ...
Fast Optical Communication Components
... The Numerical Aperture of the fiber is the sine of the maximum angle of an incident beam that can be guided in the core ...
... The Numerical Aperture of the fiber is the sine of the maximum angle of an incident beam that can be guided in the core ...
Abstract
... Measurements of the small wave tilt using the optical vortex interferometer with the Wollaston prism Agnieszka Popiołek-Masajada, Piotr Kurzynowski, Władysław A. Woźniak, Monika Borwińska, Institute of Physics, Wrocław University of Technology, Wybrzeże Wyspiańskiego 27, 50-370 Wrocław, Poland ...
... Measurements of the small wave tilt using the optical vortex interferometer with the Wollaston prism Agnieszka Popiołek-Masajada, Piotr Kurzynowski, Władysław A. Woźniak, Monika Borwińska, Institute of Physics, Wrocław University of Technology, Wybrzeże Wyspiańskiego 27, 50-370 Wrocław, Poland ...
Assembly, Alignment, and Maintenance of an Automated Laser Cutter
... How do you align an invisible laser beam optical system? Given the high powers involved, careful alignment this invisible laser was required, in part to ensure that the beam is safely contained while traversing a sequence of three mirrors, some of which are mounted to movable armatures controlled by ...
... How do you align an invisible laser beam optical system? Given the high powers involved, careful alignment this invisible laser was required, in part to ensure that the beam is safely contained while traversing a sequence of three mirrors, some of which are mounted to movable armatures controlled by ...
Answer Key Lab Microscopes and Cells
... 3. “Depth of field” refers to the thickness of the plane of focus. With a large depth of field, all of the threads can be in focused at the same time. With a narrower depth of field, only one thread or a part of one thread can be focused at a time. In order to view the other threads, you must focus ...
... 3. “Depth of field” refers to the thickness of the plane of focus. With a large depth of field, all of the threads can be in focused at the same time. With a narrower depth of field, only one thread or a part of one thread can be focused at a time. In order to view the other threads, you must focus ...
All Solid State Laser Source for Tunable Blue and Ultraviolet Radiation.
... beam inside the crystal ~waist w o 525 mm, confocal parameter b54 mm! and a second Gaussian beam with a focus between the two plane mirrors. Its cross section is slightly elliptical ~sagittal plane: w o 5198 mm, confocal parameter b5251 mm; tangential plane: w o 5154 mm, confocal parameter b5154 mm! ...
... beam inside the crystal ~waist w o 525 mm, confocal parameter b54 mm! and a second Gaussian beam with a focus between the two plane mirrors. Its cross section is slightly elliptical ~sagittal plane: w o 5198 mm, confocal parameter b5251 mm; tangential plane: w o 5154 mm, confocal parameter b5154 mm! ...
Dual-color total internal reflection fluorescence cross
... In this work, we present for the first time a dual-color excitation scheme for TIR single-molecule detection, which enables us to perform FCCS at glass-sample interfaces with high sensitivity. The improved performance of TIR-FCCS was verified on a synthetic binding assay, including dual-labeled doub ...
... In this work, we present for the first time a dual-color excitation scheme for TIR single-molecule detection, which enables us to perform FCCS at glass-sample interfaces with high sensitivity. The improved performance of TIR-FCCS was verified on a synthetic binding assay, including dual-labeled doub ...
Fast Structural and Compositional Analysis of Aged Li
... below 5 μm. SEM imaging was done at an acceleration voltage of 15 kV with the 4 quadrant angular selective ...
... below 5 μm. SEM imaging was done at an acceleration voltage of 15 kV with the 4 quadrant angular selective ...
Some Issues from Advanced Lithography General
... extremely good lens from. Let's look at the illumination source issue first: A powerful light source we need because we cannot afford to wait forever before an exposure is finished. The maximum exposure time should be below a second or so, and you simply need intense light for that. Monochromatic li ...
... extremely good lens from. Let's look at the illumination source issue first: A powerful light source we need because we cannot afford to wait forever before an exposure is finished. The maximum exposure time should be below a second or so, and you simply need intense light for that. Monochromatic li ...
Planet Earth and Its Environment A 5000-million year
... compound microscope in 1590. It was made of two convex lenses placed at either end of a wooden tube. This could magnify objects 3 to 9 times! ...
... compound microscope in 1590. It was made of two convex lenses placed at either end of a wooden tube. This could magnify objects 3 to 9 times! ...
Light Field Microscopy - Stanford Computer Graphics Laboratory
... microscopes suffer from several limitations. First, diffraction limits their spatial resolution, especially at high magnifications. This limit can be ameliorated by enlarging the acceptance angle of the objective lens (called the numerical aperture), but we reach a practical limit at about 70 degree ...
... microscopes suffer from several limitations. First, diffraction limits their spatial resolution, especially at high magnifications. This limit can be ameliorated by enlarging the acceptance angle of the objective lens (called the numerical aperture), but we reach a practical limit at about 70 degree ...
biology 163 laboratory use of the compound light microscope
... fully illuminated, circular field of view. (TIP: If you are having difficulty with this step, your eyes may be too close to the ocular lenses!) 7. Adjust the focus to accommodate for differences between your two eyes. • Close your left eye. Looking with your RIGHT EYE through the RIGHT OCULAR, focus ...
... fully illuminated, circular field of view. (TIP: If you are having difficulty with this step, your eyes may be too close to the ocular lenses!) 7. Adjust the focus to accommodate for differences between your two eyes. • Close your left eye. Looking with your RIGHT EYE through the RIGHT OCULAR, focus ...
II. Optical properties of glass
... Physical specifications of colour are associated with objects, materials, light sources, etc., based on their physical properties such as: light absorption, reflection, or emission spectra. Colour is the perception, based on the varying sensitivity of different types of cone and rods cells in the re ...
... Physical specifications of colour are associated with objects, materials, light sources, etc., based on their physical properties such as: light absorption, reflection, or emission spectra. Colour is the perception, based on the varying sensitivity of different types of cone and rods cells in the re ...
Optical Scientist - Corning
... Design, execute and support ongoing projects in the general area of light management, waveguides and devices. Specific projects include characterization and optimization of light scattering from thin films, measurement of glass optical properties, fiber optics and optical communications. Areas of sp ...
... Design, execute and support ongoing projects in the general area of light management, waveguides and devices. Specific projects include characterization and optimization of light scattering from thin films, measurement of glass optical properties, fiber optics and optical communications. Areas of sp ...
852_1.pdf
... band. High water content of the gel samples allows use of a Ho:YAG laser at wavelength ^k-2.1 (im (water absorption coefficient a = 5.9 mm"^?]). Photoexcitation relaxation results in a localized temperature increase and the resulting thermoelastic expansion creates an inhomogeneous deformation that ...
... band. High water content of the gel samples allows use of a Ho:YAG laser at wavelength ^k-2.1 (im (water absorption coefficient a = 5.9 mm"^?]). Photoexcitation relaxation results in a localized temperature increase and the resulting thermoelastic expansion creates an inhomogeneous deformation that ...
Scanning transmission soft x-ray microscopy at beamline X
... preservation in biological specimens8. This system has also been used for demonstrations of tomographic imaging, including a reconstruction of a frozen hydrated fibroblast at approximately 100x100x250 nm resolution9. Recent studies with the cryoSTXM by T. Beetz of Stony Brook have centered on a more ...
... preservation in biological specimens8. This system has also been used for demonstrations of tomographic imaging, including a reconstruction of a frozen hydrated fibroblast at approximately 100x100x250 nm resolution9. Recent studies with the cryoSTXM by T. Beetz of Stony Brook have centered on a more ...
General Introduction of Optical
... Differences between biomedical and materials microscopy involves how the microscope projects light onto the sample. Classical biological microscope: thin specimen; light is transmitted through the sample, focused with the objective and then passed into the eyepieces of the microscope. Diascopic ...
... Differences between biomedical and materials microscopy involves how the microscope projects light onto the sample. Classical biological microscope: thin specimen; light is transmitted through the sample, focused with the objective and then passed into the eyepieces of the microscope. Diascopic ...
Confocal microscopy
Confocal microscopy is an optical imaging technique for increasing optical resolution and contrast of a micrograph by means of adding a spatial pinhole placed at the confocal plane of the lens to eliminate out-of-focus light. It enables the reconstruction of three-dimensional structures from the obtained images. This technique has gained popularity in the scientific and industrial communities and typical applications are in life sciences, semiconductor inspection and materials science.