Lucifer Yellow Uptake in Cells and Protoplasts of Daucas carota
... which allows for optical sectioning of a specimen and electronic adjustment of its contrast (Robert-Nicoud, Arndt-Jovin, Schormann, and Jovin, 1988). The application of LSM in plants has hitherto been restricted to an analysis of the arrangement of cytoskeletal structures and the endoplasmic reticul ...
... which allows for optical sectioning of a specimen and electronic adjustment of its contrast (Robert-Nicoud, Arndt-Jovin, Schormann, and Jovin, 1988). The application of LSM in plants has hitherto been restricted to an analysis of the arrangement of cytoskeletal structures and the endoplasmic reticul ...
A guide to super-resolution fluorescence microscopy
... Point spread function and the significance of convolution The process of fluorescence imaging with a well-designed microscope is somewhat similar to painting the perfect object structure with a fuzzy brush. The shape (or rather the intensity distribution) of this brush is called the point spread fun ...
... Point spread function and the significance of convolution The process of fluorescence imaging with a well-designed microscope is somewhat similar to painting the perfect object structure with a fuzzy brush. The shape (or rather the intensity distribution) of this brush is called the point spread fun ...
Chapter 4: Microscopy, Staining, and Classification
... • Aperture of lens: Ability to gather light ...
... • Aperture of lens: Ability to gather light ...
Opportunities to Explore Plant Membrane
... 10 to 200 nm in diameter. Even with perfect optics, light can only be focused to a fuzzy spot, called a point spread function (PSF). If two fluorophores are in close proximity, their PSFs in the image plane may overlap to such an extent that it is impossible to distinguish the two spots. Diffraction ...
... 10 to 200 nm in diameter. Even with perfect optics, light can only be focused to a fuzzy spot, called a point spread function (PSF). If two fluorophores are in close proximity, their PSFs in the image plane may overlap to such an extent that it is impossible to distinguish the two spots. Diffraction ...
High resolution spectral self-interference fluorescence microscopy
... The advantage of light microscopy over electron and scanned probe microscopy is the ability to image the interior of biological specimens. Light would be an ideal carrier of microscopic information if it weren’t for the resolution limit. But because of diffraction, standard light microscopes are not ...
... The advantage of light microscopy over electron and scanned probe microscopy is the ability to image the interior of biological specimens. Light would be an ideal carrier of microscopic information if it weren’t for the resolution limit. But because of diffraction, standard light microscopes are not ...
Speed of Light Measurement Utilizing Octagonal
... lens ÒaÓ was the most important factor in the entire experiment. If the lens was placed after the rotating mirror no shifts will ever be seen. This is because the shifts are so small that they are well within the paraxial approximation. The lens therefore sends all of the ÒparallelÓ rays to the sam ...
... lens ÒaÓ was the most important factor in the entire experiment. If the lens was placed after the rotating mirror no shifts will ever be seen. This is because the shifts are so small that they are well within the paraxial approximation. The lens therefore sends all of the ÒparallelÓ rays to the sam ...
(Digital Micro-Mirror Device) Based Multi-Object
... The design in Figure 2 will be further evaluated, assembled and integrated together with an illumination module consisting of a halogen lamp, a mixing rod, the DMD and a projection lens. To collect as much light as needed, fast lenses are preferred, and these tend to be spacious. Measurement time, g ...
... The design in Figure 2 will be further evaluated, assembled and integrated together with an illumination module consisting of a halogen lamp, a mixing rod, the DMD and a projection lens. To collect as much light as needed, fast lenses are preferred, and these tend to be spacious. Measurement time, g ...
CMU3 - Fast and Simple High-Resolution Optical Spectrum Analyzer
... The measurement of the spectrum of optical sources is very important in science and technology. So, for instance in optical communication and transmission systems. Due to the nonlinear characteristics of optical components in such a system it is of prime importance to know the optical transmission s ...
... The measurement of the spectrum of optical sources is very important in science and technology. So, for instance in optical communication and transmission systems. Due to the nonlinear characteristics of optical components in such a system it is of prime importance to know the optical transmission s ...
Microbiology Lab 1 Examination of Bacteria
... field. Background is dark and the object is bright. An annular stop ring permits light coming from the outside of the beam. When light from the stop is deflected and deviated by the object can it be seen. Advantageous for viewing thin bacteria (Ie. Treponema pallidum) Disadvantage: internal structur ...
... field. Background is dark and the object is bright. An annular stop ring permits light coming from the outside of the beam. When light from the stop is deflected and deviated by the object can it be seen. Advantageous for viewing thin bacteria (Ie. Treponema pallidum) Disadvantage: internal structur ...
Chapter 2 System Evaluation
... Data The NBS-1952 Resolution Test Chart is described in the : NBS circular 533, 1953 in the section titled Method of Determining the Resolution Power of Photographic Lenses. The design features of this target reduce edge effects, minimize spurious resolution and permit single pass scanning. Notes Th ...
... Data The NBS-1952 Resolution Test Chart is described in the : NBS circular 533, 1953 in the section titled Method of Determining the Resolution Power of Photographic Lenses. The design features of this target reduce edge effects, minimize spurious resolution and permit single pass scanning. Notes Th ...
Download PDF
... The inset of Fig. 1 describes the four polarization combinations. The four complex elements of the Jones matrix are obtained by inverting the 4 ⫻ 4 matrix 共C兲 in Eq. (5). The constants C1 and C2 are retrieved by performing the measurement with no sample, i.e., with J as the identity 2 ⫻ 2 matrix. Th ...
... The inset of Fig. 1 describes the four polarization combinations. The four complex elements of the Jones matrix are obtained by inverting the 4 ⫻ 4 matrix 共C兲 in Eq. (5). The constants C1 and C2 are retrieved by performing the measurement with no sample, i.e., with J as the identity 2 ⫻ 2 matrix. Th ...
無投影片標題
... The pupil function is a powerful way to understand image formation. A phase retrieved pupil function can be used to calculate PSFs that contain key features observed in the measured PSF’s that are not represented in simulated PSFs. ...
... The pupil function is a powerful way to understand image formation. A phase retrieved pupil function can be used to calculate PSFs that contain key features observed in the measured PSF’s that are not represented in simulated PSFs. ...
Analysis of Materials Physical Properties The nano-scale
... In-situ observation of electrochemical process is available. Keysight pioneered the best commercial ECSTM/ECAFM designed with ease of use and highest resolution for in situ imaging of electrochemical process. The latest addition of SECM*2 combined with AFM can sense redox reactions at the electrode ...
... In-situ observation of electrochemical process is available. Keysight pioneered the best commercial ECSTM/ECAFM designed with ease of use and highest resolution for in situ imaging of electrochemical process. The latest addition of SECM*2 combined with AFM can sense redox reactions at the electrode ...
P - University of South Florida
... is located is called the scanning plane and its normal direction is defined as the scanning direction in this paper. In most of the 3D microscopy systems including the OCT and the WSDIH, the 3D volume is reconstructed as a set of scanning planes with the scanning direction along the optical axis of ...
... is located is called the scanning plane and its normal direction is defined as the scanning direction in this paper. In most of the 3D microscopy systems including the OCT and the WSDIH, the 3D volume is reconstructed as a set of scanning planes with the scanning direction along the optical axis of ...
File - ce
... Semiconductor lasers are actually solid-state lasers, too, but because semiconductor lasers have a different mode of laser operation, they have a different name. ...
... Semiconductor lasers are actually solid-state lasers, too, but because semiconductor lasers have a different mode of laser operation, they have a different name. ...
BPM Blatt 7
... Answer the following questions with the help of the review: Question a) Why does an optical trap require an objective with a high numerical aperture? Answer a) When a dielectric sphere is placed in a light gradient, the sum of all rays passing through it generates an imbalance in force, tending to p ...
... Answer the following questions with the help of the review: Question a) Why does an optical trap require an objective with a high numerical aperture? Answer a) When a dielectric sphere is placed in a light gradient, the sum of all rays passing through it generates an imbalance in force, tending to p ...
LM Ch 8: Bright Field
... from one to another, even to the oil objectives. The idea of parfocality of objectives was invented by Abbe. One precaution: avoid getting oil on your dry objectives. This will make an image through them very hazy and irregular. Remember to adjust the field iris and the aperture iris when changing f ...
... from one to another, even to the oil objectives. The idea of parfocality of objectives was invented by Abbe. One precaution: avoid getting oil on your dry objectives. This will make an image through them very hazy and irregular. Remember to adjust the field iris and the aperture iris when changing f ...
Miniaturized modules for light sheet microscopy with low chromatic
... concave-convex lens – of 25 mm focal length (Edmund Optics, #68-160, N-BK7/N-SF5) a numerical aperture AN = 0.08 is attained when using the relation AN = sinα ࣈ tanα = A/2f’ for small angles. The resulting beam waist d = λ/AN = 5.9 μm is defined by the thinnest line described by the distance between ...
... concave-convex lens – of 25 mm focal length (Edmund Optics, #68-160, N-BK7/N-SF5) a numerical aperture AN = 0.08 is attained when using the relation AN = sinα ࣈ tanα = A/2f’ for small angles. The resulting beam waist d = λ/AN = 5.9 μm is defined by the thinnest line described by the distance between ...
"Contrast Enhancement in Light Microscopy". In: Current Protocols in
... oldest instruments of scientific discovery, continue to be key tools in both biomedical research and routine diagnosis. This remains true despite the development of a wide range of new imaging technologies, many with far greater resolution—ranging from electron microscopes to the multitude of scanni ...
... oldest instruments of scientific discovery, continue to be key tools in both biomedical research and routine diagnosis. This remains true despite the development of a wide range of new imaging technologies, many with far greater resolution—ranging from electron microscopes to the multitude of scanni ...
Methods_Mol._Biol._591_185-199
... Mowiol, TDE does not harden. Samples need to be sealed with nail polish. The properties of some fluorophores are changed in TDE: Especially the absorption and emission spectra, but also the quantum efficiency and bleaching properties may be altered when the samples are mounted in TDE. ...
... Mowiol, TDE does not harden. Samples need to be sealed with nail polish. The properties of some fluorophores are changed in TDE: Especially the absorption and emission spectra, but also the quantum efficiency and bleaching properties may be altered when the samples are mounted in TDE. ...
Light Microscopy
... some feeling for depth of field numbers, it can be mentioned that for N.A.obj = 1.3 (with matching N.A.cond) a specimen thickness of only about 0.3 μm can be sharply imaged. For numerical apertures of 0.6 and 0.3, the corresponding numbers will be about 1.5 and 7 μm respectively. Also in these cases ...
... some feeling for depth of field numbers, it can be mentioned that for N.A.obj = 1.3 (with matching N.A.cond) a specimen thickness of only about 0.3 μm can be sharply imaged. For numerical apertures of 0.6 and 0.3, the corresponding numbers will be about 1.5 and 7 μm respectively. Also in these cases ...
optical cavity
... The geometry (resonator type) must be chosen so that the beam remains stable (that the size of the beam does not continually grow with multiple reflections, as shown below. ...
... The geometry (resonator type) must be chosen so that the beam remains stable (that the size of the beam does not continually grow with multiple reflections, as shown below. ...
Slide 1
... Abstract: Acousto-optic (AO) imaging is a new dual-wave modality that combines ultrasound with diffuse light to achieve deep-tissue imaging of optical properties with the spatial resolution of ultrasound. In this technique, the sample is simultaneously insonified by an ultrasound beam and illuminate ...
... Abstract: Acousto-optic (AO) imaging is a new dual-wave modality that combines ultrasound with diffuse light to achieve deep-tissue imaging of optical properties with the spatial resolution of ultrasound. In this technique, the sample is simultaneously insonified by an ultrasound beam and illuminate ...
Many other important inventions involve the use of
... light and a system of lenses to magnify images of small samples. Optical microscopes are the oldest and simplest of the microscopes. Digital microscopes are now available which use a CCD camera to examine a sample, and the image is shown directly on a computer screen without the need for optics such ...
... light and a system of lenses to magnify images of small samples. Optical microscopes are the oldest and simplest of the microscopes. Digital microscopes are now available which use a CCD camera to examine a sample, and the image is shown directly on a computer screen without the need for optics such ...
Confocal microscopy
Confocal microscopy is an optical imaging technique for increasing optical resolution and contrast of a micrograph by means of adding a spatial pinhole placed at the confocal plane of the lens to eliminate out-of-focus light. It enables the reconstruction of three-dimensional structures from the obtained images. This technique has gained popularity in the scientific and industrial communities and typical applications are in life sciences, semiconductor inspection and materials science.