Microscope Basics
... What can you do with a microscope ? One could write a book answering this question but briefly – a microscope can be used for numerous things: hobbyists have an interest in microscopic worlds from learning and having fun to using a microscope for the study of stamps, coins, gems, insects, etc ch ...
... What can you do with a microscope ? One could write a book answering this question but briefly – a microscope can be used for numerous things: hobbyists have an interest in microscopic worlds from learning and having fun to using a microscope for the study of stamps, coins, gems, insects, etc ch ...
Broadband Polarizationmaintaining Fiber Optics
... lens. This lens was specially designed to minimize the chromatic focal shift for the wavelength range 400 – 660 nm as well as for spherical aberration, so that a highly efficient fiber-coupling can be achieved over the total wavelength range. The resulting combined laser light is then coupled into, ...
... lens. This lens was specially designed to minimize the chromatic focal shift for the wavelength range 400 – 660 nm as well as for spherical aberration, so that a highly efficient fiber-coupling can be achieved over the total wavelength range. The resulting combined laser light is then coupled into, ...
Lithography - 123seminarsonly.com
... The first pattern transferred to a wafer usually includes a set of alignment marks, which are high precision features that are used as the reference when positioning subsequent patterns, to the first pattern Often alignment marks are included in other patterns, as the original alignment marks may be ...
... The first pattern transferred to a wafer usually includes a set of alignment marks, which are high precision features that are used as the reference when positioning subsequent patterns, to the first pattern Often alignment marks are included in other patterns, as the original alignment marks may be ...
Studying the temperature-dependent events of live cells under
... The major difference between the temperature control system reported here and other commercially available ones is that the formal system does not rely on a bright field optical microscope to examine the cell shape. Instead, the cell shape is outlined by the red fluorescence of the DsRed1-N1 express ...
... The major difference between the temperature control system reported here and other commercially available ones is that the formal system does not rely on a bright field optical microscope to examine the cell shape. Instead, the cell shape is outlined by the red fluorescence of the DsRed1-N1 express ...
Daily Quizzes for Laser Technology
... 2. Planetariums use_________ to display designs on a screen that are synchronized with music. ...
... 2. Planetariums use_________ to display designs on a screen that are synchronized with music. ...
Physical Optics - Haverford College
... so it reforms into an image of the diffraction grating. We will see shortly how this simple experiment can be used to understand how imaging works from a wave perspective. First, let’s recall how periodic waves are represented. Most of us are familiar with repetitions in time, such as periodic oscil ...
... so it reforms into an image of the diffraction grating. We will see shortly how this simple experiment can be used to understand how imaging works from a wave perspective. First, let’s recall how periodic waves are represented. Most of us are familiar with repetitions in time, such as periodic oscil ...
Two-photon ablation with 1278nm laser radiation
... The interaction of light with biological matter such as tissue, cells and intracellular bodies like organelles and chromosomes is highly wavelength dependent. It is also often necessary to use complex optics to deliver the light and when, as is becoming more and more common, short pulses are used, t ...
... The interaction of light with biological matter such as tissue, cells and intracellular bodies like organelles and chromosomes is highly wavelength dependent. It is also often necessary to use complex optics to deliver the light and when, as is becoming more and more common, short pulses are used, t ...
Chapter 1 Histology and its methods of study
... allows the direct observation of the behavior of living cells under the phase contrast microscope. Many experiments that cannot be performed in the living animal can be accomplished in vitro. The cells and tissues are grown in complex solutions of known composition (salts, amino acids, vitamins) to ...
... allows the direct observation of the behavior of living cells under the phase contrast microscope. Many experiments that cannot be performed in the living animal can be accomplished in vitro. The cells and tissues are grown in complex solutions of known composition (salts, amino acids, vitamins) to ...
Quantitative evaluation of light microscopes based on image
... filters are used, each with a bandwidth of 10 nm. The center wavelengths of these filters are 400 nm, 500 nm, 600 nm, and 700 nm (Andover Corporation, Salem, NH, USA). 3. Procedures In this section the variety of test procedures that use digital image processing techniques will be described and the ...
... filters are used, each with a bandwidth of 10 nm. The center wavelengths of these filters are 400 nm, 500 nm, 600 nm, and 700 nm (Andover Corporation, Salem, NH, USA). 3. Procedures In this section the variety of test procedures that use digital image processing techniques will be described and the ...
Sample preparation for Transmission electron microscopy (TEM
... conventional optical microscopy. Because the wavelength of electrons is much smaller than that of light, the optimal resolution attainable for TEM images is many orders of magnitude better than that from a light microscope. ...
... conventional optical microscopy. Because the wavelength of electrons is much smaller than that of light, the optimal resolution attainable for TEM images is many orders of magnitude better than that from a light microscope. ...
Three Lasers Converging at a Focal Point : A Demonstration
... The point at which parallel light rays meet is called the focal point. The distance from the lens to the point where the light rays meet is called the focal length. What You Need For the class: 3 laser pointers 1 acrylic block 1 velum screen or a wall 1 large positive lens mister For e ...
... The point at which parallel light rays meet is called the focal point. The distance from the lens to the point where the light rays meet is called the focal length. What You Need For the class: 3 laser pointers 1 acrylic block 1 velum screen or a wall 1 large positive lens mister For e ...
Imaging of Intrinsic Signals in the Retina
... Research Site: University of California, Berkeley Research Advisor: Austin Roorda Research Supervisor: Kate Grieve Home Institution: DePaul University Adaptive optics (AO) is used to correct ocular aberrations of the human eye and so improves lateral and axial resolution and brightness of images of ...
... Research Site: University of California, Berkeley Research Advisor: Austin Roorda Research Supervisor: Kate Grieve Home Institution: DePaul University Adaptive optics (AO) is used to correct ocular aberrations of the human eye and so improves lateral and axial resolution and brightness of images of ...
Imaging properties of a metamaterial superlens
... Metamaterials have opened an exciting gateway to create unprecedented physical properties and functionality unattainable from naturally existing materials. The ‘‘atoms’’ and ‘‘molecules’’ in metamaterials can be tailored in shape and size; the lattice constant and interatomic interaction can be arti ...
... Metamaterials have opened an exciting gateway to create unprecedented physical properties and functionality unattainable from naturally existing materials. The ‘‘atoms’’ and ‘‘molecules’’ in metamaterials can be tailored in shape and size; the lattice constant and interatomic interaction can be arti ...
The Very Basics of Geometric Optics 5.1.2 Basic Geometric Optics
... The NA for a single lens is roughly the quotient of (possibly aperture defined) diameter / focal length; i.e. a crude measure of the size of the lens; see the picture below. Of course, lenses with small NA will not suffer much from spherical aberration but will also not transmit much light and thus ...
... The NA for a single lens is roughly the quotient of (possibly aperture defined) diameter / focal length; i.e. a crude measure of the size of the lens; see the picture below. Of course, lenses with small NA will not suffer much from spherical aberration but will also not transmit much light and thus ...
apo-a1 fluorescenct localization
... concentrations (.1uM for time course studies and .125uM for others) and stored in the freezer until needed. We obtained cells by simply swabbing cheek epithelial cells after cleansing and then layering them onto a cover slip. The cells were then covered with in media at 37C. Our experiments were co ...
... concentrations (.1uM for time course studies and .125uM for others) and stored in the freezer until needed. We obtained cells by simply swabbing cheek epithelial cells after cleansing and then layering them onto a cover slip. The cells were then covered with in media at 37C. Our experiments were co ...
Document
... Diffraction is one particular type of wave interference, caused by the partial obstruction or lateral restriction of a wave; another example… ...
... Diffraction is one particular type of wave interference, caused by the partial obstruction or lateral restriction of a wave; another example… ...
The Fresnel Biprism
... points showing the imaginary sources could be seen on the screen. The distance between the imaginary sources was found to be a = 0.06 ± 0.02cm. There is no real way to compare this answer to a reference source as each optical system tends to be different in one way or another however this number was ...
... points showing the imaginary sources could be seen on the screen. The distance between the imaginary sources was found to be a = 0.06 ± 0.02cm. There is no real way to compare this answer to a reference source as each optical system tends to be different in one way or another however this number was ...
LM 3188 - LASER DUST MONITOR
... PRINCIPLE OF OPERATION: The measurment system is based on the single pass method. The laser beam crosses the measuring section once only and the receiver unit measures and evaluates the laser beam's weakening caused by the dust content. The optical value is converted to a mass value with the help of ...
... PRINCIPLE OF OPERATION: The measurment system is based on the single pass method. The laser beam crosses the measuring section once only and the receiver unit measures and evaluates the laser beam's weakening caused by the dust content. The optical value is converted to a mass value with the help of ...
Total Internal Reflection Microscopy
... the current TIRM flowcell. A syringe is used to inject salt solution containing latex particles (typically 3 to 30 mm in diameter) between two microscope slides optically coupled to a dove-tail prism. The two slides are held about 1 mm apart using an o-ring as a spacer. After 10-20 minutes, the part ...
... the current TIRM flowcell. A syringe is used to inject salt solution containing latex particles (typically 3 to 30 mm in diameter) between two microscope slides optically coupled to a dove-tail prism. The two slides are held about 1 mm apart using an o-ring as a spacer. After 10-20 minutes, the part ...
Microscope Project for Undergraduate Laboratories Abstract
... inch to one inch range were used for fine focus motion. The translation stages can be placed under the objective or the sample holder on the rail. Modern light microscopes use CCD or CMOS cameras to capture images (Fig. 1). We chose inexpensive but small and powerful CMOS cameras to capture images w ...
... inch to one inch range were used for fine focus motion. The translation stages can be placed under the objective or the sample holder on the rail. Modern light microscopes use CCD or CMOS cameras to capture images (Fig. 1). We chose inexpensive but small and powerful CMOS cameras to capture images w ...
A tutorial for designing fundamental imaging systems
... We should decide magnification of designed system according to the resolution of the image. We should decide it at first. Magnification for fundamental optical system is shown in Figure.3.1. There are 2 lenses and object with height of ho. Object is placed at front focal plane of an objective lens, ...
... We should decide magnification of designed system according to the resolution of the image. We should decide it at first. Magnification for fundamental optical system is shown in Figure.3.1. There are 2 lenses and object with height of ho. Object is placed at front focal plane of an objective lens, ...
PDF
... the computer. This modulated light beam is imaged at the back aperture of the microscope objective acting as a Fourier transform lens. Each pixel on the SLM can spatially modulate the local phase of the incident beam in the range of 0 to 2 at the operating wavelength of 1064nm with an effective ref ...
... the computer. This modulated light beam is imaged at the back aperture of the microscope objective acting as a Fourier transform lens. Each pixel on the SLM can spatially modulate the local phase of the incident beam in the range of 0 to 2 at the operating wavelength of 1064nm with an effective ref ...
X-ray crystallography electron microscopy and electron diffraction
... main sources of structural information in biological systems. Both methods can reach atomic resolution. X-ray crystallography is the method of choice if 3D-crystals of at least 20x20x20μm3 (containing > 108 ordered identical molecules) can be made. For single particles or larger systems that are ...
... main sources of structural information in biological systems. Both methods can reach atomic resolution. X-ray crystallography is the method of choice if 3D-crystals of at least 20x20x20μm3 (containing > 108 ordered identical molecules) can be made. For single particles or larger systems that are ...
Lab Module 4 - philipdarrenjones.com
... Obtain a slide labeled: Bacteria, Yeast, and Blood. Perform the following steps to learn and practice proper focusing technique: 1. If your microscope has a diaphragm, close the diaphragm all the way down. 2. Set the scanning lens (the smallest stage objective) in place. Note the large distance betw ...
... Obtain a slide labeled: Bacteria, Yeast, and Blood. Perform the following steps to learn and practice proper focusing technique: 1. If your microscope has a diaphragm, close the diaphragm all the way down. 2. Set the scanning lens (the smallest stage objective) in place. Note the large distance betw ...
3D AND MULTISPECTRAL IMAGING FOR SUBCUTANEOUS
... Multispectral imaging is commonly used to obtain reflectance measurements of an object in several spectral bands. As a result, each pixel of the image is expected to have specific intensity values over the light spectrum, corresponding to the so called spectral signature. In our experiment, the skin ...
... Multispectral imaging is commonly used to obtain reflectance measurements of an object in several spectral bands. As a result, each pixel of the image is expected to have specific intensity values over the light spectrum, corresponding to the so called spectral signature. In our experiment, the skin ...
Confocal microscopy
Confocal microscopy is an optical imaging technique for increasing optical resolution and contrast of a micrograph by means of adding a spatial pinhole placed at the confocal plane of the lens to eliminate out-of-focus light. It enables the reconstruction of three-dimensional structures from the obtained images. This technique has gained popularity in the scientific and industrial communities and typical applications are in life sciences, semiconductor inspection and materials science.