The Microscope: Window on an Invisible Realm
... o Fluorescence – the ability to absorb short wavelengths of light (UV)and give off that energy as the longer wavelength (visible) of light o Fluorochromes – fluorescent dyes – stain specimens o Fluorescent-antibody technique – antibodies can be tagged with fluorescent dye o specific antibodies can b ...
... o Fluorescence – the ability to absorb short wavelengths of light (UV)and give off that energy as the longer wavelength (visible) of light o Fluorochromes – fluorescent dyes – stain specimens o Fluorescent-antibody technique – antibodies can be tagged with fluorescent dye o specific antibodies can b ...
Optical Imaging Tools for Early Detection
... What is the “Price” for a Fully Functional Optical Design? This is the size of lens needed for achieving the FOV of a fly and also the resolution of ...
... What is the “Price” for a Fully Functional Optical Design? This is the size of lens needed for achieving the FOV of a fly and also the resolution of ...
View/Open
... to key improvements, it is now possible to obtain speeds of image acquisition of 120 images/s or even higher, and to have multispectral imaging due to minimization of spectral emission overlap. Microscope systems incorporating these modifications include commercial light scanning confocals, spinnin ...
... to key improvements, it is now possible to obtain speeds of image acquisition of 120 images/s or even higher, and to have multispectral imaging due to minimization of spectral emission overlap. Microscope systems incorporating these modifications include commercial light scanning confocals, spinnin ...
Wide-field extended-resolution fluorescence
... and provides lateral resolution approaching 100 nm. A schematic of the SW-SPRF microscope is shown in Fig. 1. In this configuration, counter-propagating SPR waves are launched through a high NA objective lens, which interfere and excite fluorophores close to the metal interface. Emission light is co ...
... and provides lateral resolution approaching 100 nm. A schematic of the SW-SPRF microscope is shown in Fig. 1. In this configuration, counter-propagating SPR waves are launched through a high NA objective lens, which interfere and excite fluorophores close to the metal interface. Emission light is co ...
Midterm Exam
... c. Put a focusing lens immediately after the output coupler of the resonator as shown below. Design the lens to minimize the focused spot size: i. What is the minimum spot produced by this configuration? ii. Where is the minimum spot size located? ...
... c. Put a focusing lens immediately after the output coupler of the resonator as shown below. Design the lens to minimize the focused spot size: i. What is the minimum spot produced by this configuration? ii. Where is the minimum spot size located? ...
Lecture 1 TEM
... It is an imaging mode of the transmission electron microscope (TEM) that allows for direct imaging of the atomic structure of the sample. HRTEM can provide structural information at better than 0.2 nm spatial resolution. As a result it is suitable for the study on atomic scale of the materials like ...
... It is an imaging mode of the transmission electron microscope (TEM) that allows for direct imaging of the atomic structure of the sample. HRTEM can provide structural information at better than 0.2 nm spatial resolution. As a result it is suitable for the study on atomic scale of the materials like ...
Single-pixel infrared and visible microscope
... rate and N is the number of a pixels in the image. For our particular DMD, the pattern display rate of 22 kHz results in a frame rate of 10 Hz at 32 × 32 and 0.6 Hz at 128 × 128. We expand upon this “standard” operational mode to provide a user-friendly “adaptive” mode that provides the benefits of ...
... rate and N is the number of a pixels in the image. For our particular DMD, the pattern display rate of 22 kHz results in a frame rate of 10 Hz at 32 × 32 and 0.6 Hz at 128 × 128. We expand upon this “standard” operational mode to provide a user-friendly “adaptive” mode that provides the benefits of ...
full press release.
... Dr Kurt Anderson heads up the Light Microscopy Science Technology Platform at the Francis Crick Institute. They specialise in high resolution, high throughput, fluorescence microscopy, three dimensional (3D) digital imaging, time-lapse and low-light-level imaging. The facility allows access to state ...
... Dr Kurt Anderson heads up the Light Microscopy Science Technology Platform at the Francis Crick Institute. They specialise in high resolution, high throughput, fluorescence microscopy, three dimensional (3D) digital imaging, time-lapse and low-light-level imaging. The facility allows access to state ...
Wet electron microscopy with quantum dots Winston Timp1, Nicki
... clearly that it is easily possible to image the same sample in both electron and light microscopy. It is important to note, however, that EM was destructive to the sample at certain intensities. When imaging at high magnification, the rapid scanning of the beam over the same area seems to damage the ...
... clearly that it is easily possible to image the same sample in both electron and light microscopy. It is important to note, however, that EM was destructive to the sample at certain intensities. When imaging at high magnification, the rapid scanning of the beam over the same area seems to damage the ...
Fiber Optic Light Sources - Electrical and Computer
... threshold will result in spontaneous emission only At currents below threshold LDs operate as ELEDs LDs need more current to operate and more current means more complex drive circuitry with higher heat dissipation Laser diodes are much more temperature sensitive than LEDs ...
... threshold will result in spontaneous emission only At currents below threshold LDs operate as ELEDs LDs need more current to operate and more current means more complex drive circuitry with higher heat dissipation Laser diodes are much more temperature sensitive than LEDs ...
Introduction to Optics Frank L. Pedrotti Leno M. Pedrotti Leno S
... England and Associated Companies throughout the world Visit us on the World Wide Web at: www.pearsoned.co.uk © Pearson Education Limited 2014 All rights reserved. No part of this publication may be reproduced, stored in a retrieval system, or transmitted in any form or by any means, electronic, mech ...
... England and Associated Companies throughout the world Visit us on the World Wide Web at: www.pearsoned.co.uk © Pearson Education Limited 2014 All rights reserved. No part of this publication may be reproduced, stored in a retrieval system, or transmitted in any form or by any means, electronic, mech ...
Module Descriptor - What is FlexiLearn?
... This module covers the fundamentals of optic al design and image formation. The course will provide graduate students using optical systems with an in-depth knowledge of optical design. In particular, the course focuses on: Basic Concepts of Geometrical Optics Basic concepts of image formation ...
... This module covers the fundamentals of optic al design and image formation. The course will provide graduate students using optical systems with an in-depth knowledge of optical design. In particular, the course focuses on: Basic Concepts of Geometrical Optics Basic concepts of image formation ...
Nanofabrication with Holographic Optical Tweezers
... them from filling. Fortunately, a convenient solution presents itself. The object lens, L2, of the telescope forms a conjugate (OP∗ ) to OP in which each trapping beam comes to a separate focus. Their separation in OP∗ , moreover, is magnified by the ratio of L2’s focal length to the objective’s. B ...
... them from filling. Fortunately, a convenient solution presents itself. The object lens, L2, of the telescope forms a conjugate (OP∗ ) to OP in which each trapping beam comes to a separate focus. Their separation in OP∗ , moreover, is magnified by the ratio of L2’s focal length to the objective’s. B ...
Section 1 Supplement
... The progress of rays will be used to determine image formation for lenses, mirrors and other optical elements. An object is anything that is being viewed, e.g., when one looks at a tree through a lens, with a mirror or any other optical device the tree is referred to an optical object. Object Distan ...
... The progress of rays will be used to determine image formation for lenses, mirrors and other optical elements. An object is anything that is being viewed, e.g., when one looks at a tree through a lens, with a mirror or any other optical device the tree is referred to an optical object. Object Distan ...
Abstract : Fiber interfaces between single atoms and single photons Sébastien Garcia,
... miniaturization, stability et flexibility provided by optical fibers as light wave-guides, we present two experiments where optical fibers are used as interfaces for single atoms trapping and single photons collection into their guided modes. The first experiment combines a singlemode fiber with an ...
... miniaturization, stability et flexibility provided by optical fibers as light wave-guides, we present two experiments where optical fibers are used as interfaces for single atoms trapping and single photons collection into their guided modes. The first experiment combines a singlemode fiber with an ...
Surface Plasmons
... Single molecule detection on cells by NSOM. This figure shows a 40 nm optical resolution near-field ‘zoom-in’ on the indicated area (3.2 mm2) in the bright-field image of a fibroblast expressing LFA-1GFP. GFP excitation is accomplished using 488 nm light (Ar-Kr laser line) linearly polarized along 9 ...
... Single molecule detection on cells by NSOM. This figure shows a 40 nm optical resolution near-field ‘zoom-in’ on the indicated area (3.2 mm2) in the bright-field image of a fibroblast expressing LFA-1GFP. GFP excitation is accomplished using 488 nm light (Ar-Kr laser line) linearly polarized along 9 ...
Material and design choices for a small movement +
... the precision optical platform needs to be able to be moved and then moved back to the same location within the range of motion throughout the life time of the system. Orientation Independence; many times it is unknown what orientation relative to the gravity field will be the final orientation of t ...
... the precision optical platform needs to be able to be moved and then moved back to the same location within the range of motion throughout the life time of the system. Orientation Independence; many times it is unknown what orientation relative to the gravity field will be the final orientation of t ...
Second Harmonic Imaging of Plant Polysaccharides
... starch grains in living algal cells by SHG, using a pulsed neodymium laser in a non-scanning microscope with a CCD camera. Using wide-field imaging in this way should give good lateral resolution, since the wavelength of the emitted light will determine the resolution, but it is not depth-selective ...
... starch grains in living algal cells by SHG, using a pulsed neodymium laser in a non-scanning microscope with a CCD camera. Using wide-field imaging in this way should give good lateral resolution, since the wavelength of the emitted light will determine the resolution, but it is not depth-selective ...
Phison Le - American Academy of Optometry
... the epithelium and will stain with NaFL/Rose Bengal. Optical coherence tomography and confocal microscopy have been used to perform non-invasive analysis of these lesions; with high sensitivity and specificity. The OCT is able to locate the layer within which the lesion resides Confocal microscopy i ...
... the epithelium and will stain with NaFL/Rose Bengal. Optical coherence tomography and confocal microscopy have been used to perform non-invasive analysis of these lesions; with high sensitivity and specificity. The OCT is able to locate the layer within which the lesion resides Confocal microscopy i ...
Focusing of light through a stratified medium: a practical
... For a NA=1.4 oil immersion objective, the lateral size difference is 15% when the specimen is placed just below the cover glass. One could argue that when using the same NA=1.4 objective to image a specimen at a depth of 15 µm, the error on the lateral resolution is only 4% (Fig. 4). However the los ...
... For a NA=1.4 oil immersion objective, the lateral size difference is 15% when the specimen is placed just below the cover glass. One could argue that when using the same NA=1.4 objective to image a specimen at a depth of 15 µm, the error on the lateral resolution is only 4% (Fig. 4). However the los ...
Diffraction and Interference
... • If the size of object is the same as the wavelength of light, the image will be blurred by diffraction. Smaller objects won’t be seen at all • No optical microscope can be built big enough or designed well enough to overcome this diffraction limit. • Electron microscopes used to “see” very small t ...
... • If the size of object is the same as the wavelength of light, the image will be blurred by diffraction. Smaller objects won’t be seen at all • No optical microscope can be built big enough or designed well enough to overcome this diffraction limit. • Electron microscopes used to “see” very small t ...
Get PDF - OSA Publishing
... of fluorophores that are in the on state, such that only a sparse subset is imaged at any one time; hence images from individual fluorophores can be discriminated spatially on wide-field detectors. SMLM achieves superresolution via the precise localization of individual molecules from their emission ...
... of fluorophores that are in the on state, such that only a sparse subset is imaged at any one time; hence images from individual fluorophores can be discriminated spatially on wide-field detectors. SMLM achieves superresolution via the precise localization of individual molecules from their emission ...
Partially coherent image formation with x
... Unlike conventional refractive lenses, the focusing properties of zone plates are based on diffraction. This implies that the focal length of a zone plate lens strongly depends on the wavelength and that multiple diffraction orders exist. In most cases only the first diffraction order is used. The d ...
... Unlike conventional refractive lenses, the focusing properties of zone plates are based on diffraction. This implies that the focal length of a zone plate lens strongly depends on the wavelength and that multiple diffraction orders exist. In most cases only the first diffraction order is used. The d ...
Tutor 4
... In the last issue, we described how defocus affects the formation of an image. This information, combined with the basic imaging theory introduced in the first two parts of this series, has given us a fairly clear picture of how lithographic imaging systems work. We are now in a position to make a l ...
... In the last issue, we described how defocus affects the formation of an image. This information, combined with the basic imaging theory introduced in the first two parts of this series, has given us a fairly clear picture of how lithographic imaging systems work. We are now in a position to make a l ...
Confocal microscopy
Confocal microscopy is an optical imaging technique for increasing optical resolution and contrast of a micrograph by means of adding a spatial pinhole placed at the confocal plane of the lens to eliminate out-of-focus light. It enables the reconstruction of three-dimensional structures from the obtained images. This technique has gained popularity in the scientific and industrial communities and typical applications are in life sciences, semiconductor inspection and materials science.