Identification of a Cis-Acting Element of ART1, a
... able to bind ART1 protein (Fig. 2B). These results indicate that the region between 2358 and 2319 in the STAR1 promoter contains cis-acting element of ART1. This is further confirmed by competition and super shift experiments (Fig. 2C, lanes 5–8). The candidate region was further narrowed by gelshif ...
... able to bind ART1 protein (Fig. 2B). These results indicate that the region between 2358 and 2319 in the STAR1 promoter contains cis-acting element of ART1. This is further confirmed by competition and super shift experiments (Fig. 2C, lanes 5–8). The candidate region was further narrowed by gelshif ...
Highly Conserved Region 141–168 of the NS1 Protein Is a New
... Dengue fever/dengue hemorrhagic fever (DHF) is the most important human arthropod-borne viral disease, and the incidence of severe and life-threatening forms of this disease, namely DHF or dengue shock syndrome, is increasing (1,2). Dengue virus (DENV) has four serotypes (DENV 1–4), all of which are ...
... Dengue fever/dengue hemorrhagic fever (DHF) is the most important human arthropod-borne viral disease, and the incidence of severe and life-threatening forms of this disease, namely DHF or dengue shock syndrome, is increasing (1,2). Dengue virus (DENV) has four serotypes (DENV 1–4), all of which are ...
1. Sucrose is a disaccharide. The diagram shows the structure of a
... Some people who have a deficiency of galactokinase suffer from cataracts. These cataracts result from an increase in the amount of water in the lens of the eye. In the absence of galactokinase, galactose is converted to galacticol. Galacticol is a soluble substance whose molecules are too large to p ...
... Some people who have a deficiency of galactokinase suffer from cataracts. These cataracts result from an increase in the amount of water in the lens of the eye. In the absence of galactokinase, galactose is converted to galacticol. Galacticol is a soluble substance whose molecules are too large to p ...
Formation of Monoterpenes in Antirrhinum majus
... to, but much more divergent from, the sequence of GGPPS (only 24 to 29% identity) (Burke et al., 1999). The two highly conserved aspartate-rich motifs observed in other prenyltransferases and determined to be involved in prenyl substrate binding (Marrero et al., 1992; Song and Poulter, 1994; Tarshis ...
... to, but much more divergent from, the sequence of GGPPS (only 24 to 29% identity) (Burke et al., 1999). The two highly conserved aspartate-rich motifs observed in other prenyltransferases and determined to be involved in prenyl substrate binding (Marrero et al., 1992; Song and Poulter, 1994; Tarshis ...
Department of Chemistry University of Manitoba Undergraduate
... CHEM 2220 (L) Introductory Organic Chemistry II: Reactivity and Synthesis .................................................... 6 CHEM 2280 (L) Physical Chemistry: Microscopic Descriptions of Matter .......................................................... 7 CHEM 2290 (L) Chemical Energetics and Dyn ...
... CHEM 2220 (L) Introductory Organic Chemistry II: Reactivity and Synthesis .................................................... 6 CHEM 2280 (L) Physical Chemistry: Microscopic Descriptions of Matter .......................................................... 7 CHEM 2290 (L) Chemical Energetics and Dyn ...
Computational Studies of Multi-Active Site Enzymes
... binding of its cognate and non-cognate Thr- and Ser-AMP substrates respectively, and (ii) mechanism of hydrolytic pre-transfer editing. In contrast to bound Thr-AMP, bound SerAMP is less constrained; i.e., greater positional variability, and as a result more waters are able to permeate the active si ...
... binding of its cognate and non-cognate Thr- and Ser-AMP substrates respectively, and (ii) mechanism of hydrolytic pre-transfer editing. In contrast to bound Thr-AMP, bound SerAMP is less constrained; i.e., greater positional variability, and as a result more waters are able to permeate the active si ...
PDF File
... hymena ribozyme numbering; Figure 1).[11] Previous functional data have shown that the replacement of the 2’-OH group at position A261 with other groups is deleterious.[12–14] Consistent with its importance in catalysis, the 2’-OH group of A261 lies near N3 of guanosine in the structural model deriv ...
... hymena ribozyme numbering; Figure 1).[11] Previous functional data have shown that the replacement of the 2’-OH group at position A261 with other groups is deleterious.[12–14] Consistent with its importance in catalysis, the 2’-OH group of A261 lies near N3 of guanosine in the structural model deriv ...
Genomics Insights esTs from seeds to Assist the selective Breeding
... fatty acid biosynthesis are expressed at a medium to high degree in the developing seed.18 This property is particularly interesting for the identification of cDNAs involved in fatty acid biosynthesis through EST profiling.19 In addition, EST sequencing, associated with real-time PCR and even pyros ...
... fatty acid biosynthesis are expressed at a medium to high degree in the developing seed.18 This property is particularly interesting for the identification of cDNAs involved in fatty acid biosynthesis through EST profiling.19 In addition, EST sequencing, associated with real-time PCR and even pyros ...
Early events in protein folding
... variants of the λ-repressor fragment, λ6-85, fold on a timescale close to the protein folding speed limit44,53,56. By tuning the protein stability through mutations, solvent or temperature, the kinetics of a downhill folding reaction can be switched from exponential to non-exponential and vice versa ...
... variants of the λ-repressor fragment, λ6-85, fold on a timescale close to the protein folding speed limit44,53,56. By tuning the protein stability through mutations, solvent or temperature, the kinetics of a downhill folding reaction can be switched from exponential to non-exponential and vice versa ...
subunits of succinyl CoA ligase of tomato
... protein of 337 amino acids and SlSCoAL b a protein of 417 amino acids. At the protein level, SlSCoAL a1 and SlSCoAL a2 exhibit 90% identity with the majority of differences being located in the first 43 amino acids, although the SlSCoAL a2 also contains additional residues at positions 27 and 41 of th ...
... protein of 337 amino acids and SlSCoAL b a protein of 417 amino acids. At the protein level, SlSCoAL a1 and SlSCoAL a2 exhibit 90% identity with the majority of differences being located in the first 43 amino acids, although the SlSCoAL a2 also contains additional residues at positions 27 and 41 of th ...
EMBL-EBI Powerpoint Presentation
... BLOSUM (BLOCKS amino-acid substitution) • Based on protein domain alignments from the ...
... BLOSUM (BLOCKS amino-acid substitution) • Based on protein domain alignments from the ...
The Emerging Role of Branched-Chain Amino Acids in Insulin
... improvement in metabolism, in spite of activated mTORC1 signaling. Deletion of mitochondrial branched-chain aminotransferase (BCATm) increased energy expenditure and improved insulin sensitivity [53]. BCATm catalyzes the transfer of an α-amino group from a BCAA to α-ketoglutarate, forming glutamate ...
... improvement in metabolism, in spite of activated mTORC1 signaling. Deletion of mitochondrial branched-chain aminotransferase (BCATm) increased energy expenditure and improved insulin sensitivity [53]. BCATm catalyzes the transfer of an α-amino group from a BCAA to α-ketoglutarate, forming glutamate ...
Contributions of direct incorporation from diet and microbial amino
... biomolecules used to biosynthesize tissues, but has been used sparingly to investigate routing in controlled experiments in comparison with bulk tissue analysis. The d13C values of individual amino acids in a single protein can vary by >20&, but similarity in the overall pattern of amino acid d13C v ...
... biomolecules used to biosynthesize tissues, but has been used sparingly to investigate routing in controlled experiments in comparison with bulk tissue analysis. The d13C values of individual amino acids in a single protein can vary by >20&, but similarity in the overall pattern of amino acid d13C v ...
Functional genomics analysis of the effects of co- decarboxylase/ornithine decarboxylase
... cytostasis, a strategy employed for treatment of West-African trypanosomiasis. In the malaria parasite, Plasmodium falciparum, polyamine biosynthesis is regulated by the uniquely bifunctional protein, Sadenosylmethionine decarboxylase/ornithine decarboxylase (PfAdoMetDC/ODC). The unique nature of th ...
... cytostasis, a strategy employed for treatment of West-African trypanosomiasis. In the malaria parasite, Plasmodium falciparum, polyamine biosynthesis is regulated by the uniquely bifunctional protein, Sadenosylmethionine decarboxylase/ornithine decarboxylase (PfAdoMetDC/ODC). The unique nature of th ...
PROTEIN PHOSPHORYLATION AND CELLULAR REGULATION, I by
... In Fall 1953, Ed Fischer joined the Department of Biochemistry at the University of Washington , where I was already a faculty member. As a graduate student in Geneva, Ed had worked on potato phosphorylase, and we thus shared a common interest in this particular enzyme. We discussed some of the puzz ...
... In Fall 1953, Ed Fischer joined the Department of Biochemistry at the University of Washington , where I was already a faculty member. As a graduate student in Geneva, Ed had worked on potato phosphorylase, and we thus shared a common interest in this particular enzyme. We discussed some of the puzz ...
Virology Journal
... enrichment in log-phase bacterial broth cultures. These three bacteriophages were classified initially within the family Siphoviridae due to their long, non-contractile tails, but their phylogenetic affiliation could not be assessed in the absence of phage genome sequence analysis [8-10]. To date no ...
... enrichment in log-phase bacterial broth cultures. These three bacteriophages were classified initially within the family Siphoviridae due to their long, non-contractile tails, but their phylogenetic affiliation could not be assessed in the absence of phage genome sequence analysis [8-10]. To date no ...
Comparitive Genomics- Final Results
... 2. Very different profile from the compared Vibrios. 3. Vibrio navarrensis is not similar to the non-human pathogenic Vibrios. 4. Blood and environmental strains of V. navarrensis are very similar. • Differences: LPS synthesis, Type-I secretion system, Permeases. 5. We still believe that these will ...
... 2. Very different profile from the compared Vibrios. 3. Vibrio navarrensis is not similar to the non-human pathogenic Vibrios. 4. Blood and environmental strains of V. navarrensis are very similar. • Differences: LPS synthesis, Type-I secretion system, Permeases. 5. We still believe that these will ...
artículo - Anales de la Real Academia Nacional de Farmacia
... 15 days with this β1-‐selective blocker simply added to drinking water (2). In the present study we test the long-‐term effects of this drug given to mice in their drinking water throughout ...
... 15 days with this β1-‐selective blocker simply added to drinking water (2). In the present study we test the long-‐term effects of this drug given to mice in their drinking water throughout ...
Sculpting the b-peptide foldamer H12 helix via a designed side
... (foldamers) are highly intriguing compounds at the interface of covalent and supramolecular (noncovalent) chemistry.1 Among foldamers based on remote intrastrand interactions, peptides containing b-amino acid residues are probably the most thoroughly studied systems.2 Their versatile secondary struc ...
... (foldamers) are highly intriguing compounds at the interface of covalent and supramolecular (noncovalent) chemistry.1 Among foldamers based on remote intrastrand interactions, peptides containing b-amino acid residues are probably the most thoroughly studied systems.2 Their versatile secondary struc ...
Amino Acids - Rose
... 1. Amino acids can be metabolized to produce energy. This is especially important during fasting, when the breakdown of muscle protein is a major source of energy and biosynthetic precursors. 2. Some amino acids act as neurotransmitters, and some act as starting materials for the biosynthesis of neu ...
... 1. Amino acids can be metabolized to produce energy. This is especially important during fasting, when the breakdown of muscle protein is a major source of energy and biosynthetic precursors. 2. Some amino acids act as neurotransmitters, and some act as starting materials for the biosynthesis of neu ...
General principles of cellular organization in the genome
... investigations, to which I contributed most substantially. In order to study basic principles of bacterial proteome organization, we used tandem affinity purification followed by mass spectrometric analysis to investigate protein complexes in a proteome-wide screen. The analysis revealed 62 homomult ...
... investigations, to which I contributed most substantially. In order to study basic principles of bacterial proteome organization, we used tandem affinity purification followed by mass spectrometric analysis to investigate protein complexes in a proteome-wide screen. The analysis revealed 62 homomult ...
Protein Profile Characterization of Donkey Milk
... considered as a potential therapeutic agent against various tumour cells 32. Furthermore it was shown that -lactalbumin possesses anti-inflammatory activity exerted by the inhibition of cyclooxygenase-2 (COX-2) and phospholipase A2 33. One of the main allergens in children is -lactoglobulin th ...
... considered as a potential therapeutic agent against various tumour cells 32. Furthermore it was shown that -lactalbumin possesses anti-inflammatory activity exerted by the inhibition of cyclooxygenase-2 (COX-2) and phospholipase A2 33. One of the main allergens in children is -lactoglobulin th ...
Investigation of Factors Affecting Opalescence and Phase
... several terminal diseases including cancer and auto-immune disorders.1 Second generation of antibody-based therapeutics such as ADCs, bispecific antibodies, engineered antibodies and antibody fragments or domains have generated quite an interest over last few years. These newer molecules have furthe ...
... several terminal diseases including cancer and auto-immune disorders.1 Second generation of antibody-based therapeutics such as ADCs, bispecific antibodies, engineered antibodies and antibody fragments or domains have generated quite an interest over last few years. These newer molecules have furthe ...
Protein–protein interaction
Protein–protein interactions (PPIs) refer to physical contacts established between two or more proteins as a result of biochemical events and/or electrostatic forces.In fact, proteins are vital macromolecules, at both cellular and systemic levels, but they rarely act alone. Diverse essential molecular processes within a cell are carried out by molecular machines that are built from a large number of protein components organized by their PPIs. Indeed, these interactions are at the core of the entire interactomics system of any living cell and so, unsurprisingly, aberrant PPIs are on the basis of multiple diseases, such as Creutzfeld-Jacob, Alzheimer's disease, and cancer.PPIs have been studied from different perspectives: biochemistry, quantum chemistry, molecular dynamics, signal transduction, among others. All this information enables the creation of large protein interaction networks – similar to metabolic or genetic/epigenetic networks – that empower the current knowledge on biochemical cascades and disease pathogenesis, as well as provide putative new therapeutic targets.