Unit 14 – Biomolecules
... carbon atoms and the functional group present in them. Monosaccharides containing an aldehyde group are known as aldoses and those containing a keto group are known as ketoses. Monosaccharides are further classified as trioses, tetroses, pentoses, hexoses, and heptoses ...
... carbon atoms and the functional group present in them. Monosaccharides containing an aldehyde group are known as aldoses and those containing a keto group are known as ketoses. Monosaccharides are further classified as trioses, tetroses, pentoses, hexoses, and heptoses ...
Intestinal peptidases form functional complexes with the neutral
... Protein complexes containing APN and other brush-border peptide hydrolases have been isolated from intestinal brush-border membranes using Blue native electrophoresis [19], but these did not seem to contain membrane transporters. There is also evidence of a role for intestinal microvillar micro-doma ...
... Protein complexes containing APN and other brush-border peptide hydrolases have been isolated from intestinal brush-border membranes using Blue native electrophoresis [19], but these did not seem to contain membrane transporters. There is also evidence of a role for intestinal microvillar micro-doma ...
Get PDF - Wiley Online Library
... N-terminally longer by 158 amino acids relative to the methionine which aligns with the first methionine of the published eIF4GI sequence (Gradi et al., 1998). We suspected that the original cDNA clone of eIF4GI (Yan et al., 1992) contained an intron, because the similarity of this clone to that of ...
... N-terminally longer by 158 amino acids relative to the methionine which aligns with the first methionine of the published eIF4GI sequence (Gradi et al., 1998). We suspected that the original cDNA clone of eIF4GI (Yan et al., 1992) contained an intron, because the similarity of this clone to that of ...
role of the proteolytic enzymes in the living organisms - IJIIT
... inactive precursors. Synthesized as trypsinogen, it requires proteolytic processing to be activated and once they activated trypsin acts specifically only on peptide bonds whose carboxyl functions are contributed by lysine or arginine residues. Proteases are responsible for the post-translational mo ...
... inactive precursors. Synthesized as trypsinogen, it requires proteolytic processing to be activated and once they activated trypsin acts specifically only on peptide bonds whose carboxyl functions are contributed by lysine or arginine residues. Proteases are responsible for the post-translational mo ...
Bacillus cereus
... B. megaterium has often been used in the laboratory, and is used as an industrial organism that is able to produce a variety of proteins and sources of bioremediation. Bacillus megaterium is a good source of industrial proteins because it is both a desirable cloning host and produces a large variat ...
... B. megaterium has often been used in the laboratory, and is used as an industrial organism that is able to produce a variety of proteins and sources of bioremediation. Bacillus megaterium is a good source of industrial proteins because it is both a desirable cloning host and produces a large variat ...
document/47414 - UvA-DARE
... characterized by normal 3-methylglutaconyl-CoA hydratase activity necessitated the development of a specific assay to measure this enzyme activity. The results are described in chapter 4. In our quest to resolve the molecular basis of 3-methylglutaconic aciduria type I another gene was encountered w ...
... characterized by normal 3-methylglutaconyl-CoA hydratase activity necessitated the development of a specific assay to measure this enzyme activity. The results are described in chapter 4. In our quest to resolve the molecular basis of 3-methylglutaconic aciduria type I another gene was encountered w ...
Channel-mediated lactic acid transport: a novel function for
... or other substrates in the presence of a second carbon source, since they cannot utilize glycerol alone [9]. However, the large number of MIPs in lactic acid bacteria suggests functions other than water and glycerol transport. Interestingly, a few MIPs have been shown to transport lactic acid in hum ...
... or other substrates in the presence of a second carbon source, since they cannot utilize glycerol alone [9]. However, the large number of MIPs in lactic acid bacteria suggests functions other than water and glycerol transport. Interestingly, a few MIPs have been shown to transport lactic acid in hum ...
Pepsinogen and Pepsin - The Journal of General Physiology
... T h e forces tending to hold the folds of proteins together in some specific fashion include hydrogen and hydrophobic bonds, electrostatic and disulfide bonds (33). The last are the strongest, but there are only three per molecule of pepsinogen, whereas the hydrogen and hydrophobic bonds are more nu ...
... T h e forces tending to hold the folds of proteins together in some specific fashion include hydrogen and hydrophobic bonds, electrostatic and disulfide bonds (33). The last are the strongest, but there are only three per molecule of pepsinogen, whereas the hydrogen and hydrophobic bonds are more nu ...
Turnover-based in vitro selection and evolution of biocatalysts from
... deactivated (step 3), and scFv antibodies (0.2 µM) were injected for 2 h (step 4). Antibody fragments TT1 and BB3 were bound at intensities of 177 RU and 123 RU, respectively. Noncovalently attached protein was washed off with buffer containing 6 M guanidinium chloride (step 5). The remaining signal ...
... deactivated (step 3), and scFv antibodies (0.2 µM) were injected for 2 h (step 4). Antibody fragments TT1 and BB3 were bound at intensities of 177 RU and 123 RU, respectively. Noncovalently attached protein was washed off with buffer containing 6 M guanidinium chloride (step 5). The remaining signal ...
Structure of the ordered hydration of amino acids
... In this paper, we studied the hydration of proteins in crystal structures at the detailed level of individual amino-acid residues. A similar approach has previously been applied in several studies in the 1980s and 1990s (Goodfellow et al., 1993; Roe & Teeter, 1993; Flanagan et al., 1995). Although t ...
... In this paper, we studied the hydration of proteins in crystal structures at the detailed level of individual amino-acid residues. A similar approach has previously been applied in several studies in the 1980s and 1990s (Goodfellow et al., 1993; Roe & Teeter, 1993; Flanagan et al., 1995). Although t ...
Computational protein design enables a novel one
... for both the benzoin and formose reaction obtained from linear fits to substrate vs. velocity profiles, as described in Materials and Methods. All fits had at least four independently measured rates with an R2 > 0.9 and are shown in SI Appendix, Fig. S1. n.d.a., no detectable activity. ...
... for both the benzoin and formose reaction obtained from linear fits to substrate vs. velocity profiles, as described in Materials and Methods. All fits had at least four independently measured rates with an R2 > 0.9 and are shown in SI Appendix, Fig. S1. n.d.a., no detectable activity. ...
MCAD - MSOE Center for BioMolecular Modeling
... Intermolecular Interactions Modeled in MCAD, FAD and ETF MCAD Active Site: Glu376, Glu199, Leu103, Ser142, Met249, Asp253, Arg388 Folding of MCAD: 2 Dimers Combine: Arg28:A and Glu86:D Tetramer: Lys304, Glu300, Gln342, Asp346, Arg383 ...
... Intermolecular Interactions Modeled in MCAD, FAD and ETF MCAD Active Site: Glu376, Glu199, Leu103, Ser142, Met249, Asp253, Arg388 Folding of MCAD: 2 Dimers Combine: Arg28:A and Glu86:D Tetramer: Lys304, Glu300, Gln342, Asp346, Arg383 ...
2nd_lecture
... Sequence Determination Frederick Sanger was the first - in 1953, he sequenced the two chains of insulin. • Sanger's results established that all of the molecules of a given protein have the same sequence • Proteins can be sequenced in two ways: - real amino acid sequencing - sequencing the correspon ...
... Sequence Determination Frederick Sanger was the first - in 1953, he sequenced the two chains of insulin. • Sanger's results established that all of the molecules of a given protein have the same sequence • Proteins can be sequenced in two ways: - real amino acid sequencing - sequencing the correspon ...
A SOLUBLE RIBONUCLEIC ACID INTERMEDIATE IN PROTEIN
... irreversibly into cY-peptide linkage in protein has been used in our laboratories for a number of years as a measure of protein synthesis. The essential components of this system are the microsomal ribonucleoprotein particles, certain enzymes derived from the soluble protein fraction, adenosine trip ...
... irreversibly into cY-peptide linkage in protein has been used in our laboratories for a number of years as a measure of protein synthesis. The essential components of this system are the microsomal ribonucleoprotein particles, certain enzymes derived from the soluble protein fraction, adenosine trip ...
Docking of B-cell epitope antigen to specific hepatitis B antibody
... The interaction of B-cell epitope peptide amino acid sequence with antibody at interatomic distance less than 5 Å showed that the interactions between the antigen and antibody had occurred only in the active site pockets of hepatitis B antibody. The docking study reveals that van der Waals forces pl ...
... The interaction of B-cell epitope peptide amino acid sequence with antibody at interatomic distance less than 5 Å showed that the interactions between the antigen and antibody had occurred only in the active site pockets of hepatitis B antibody. The docking study reveals that van der Waals forces pl ...
PDF file
... phosphatase and at least 10-fold lower than that of the dual specificity protein phosphatase Cdc14 (20). In contrast, PIR1 dephosphorylates the 59-end of RNA at rates comparable to CEL-1, which acts as a mRNA capping enzyme in vivo (2, 3). From these in vitro measurements, we conclude that PIR1 is a ...
... phosphatase and at least 10-fold lower than that of the dual specificity protein phosphatase Cdc14 (20). In contrast, PIR1 dephosphorylates the 59-end of RNA at rates comparable to CEL-1, which acts as a mRNA capping enzyme in vivo (2, 3). From these in vitro measurements, we conclude that PIR1 is a ...
BIOGRAPHICAL SKETCH Joanne I. Yeh joanneyeh Associate
... essential monotopic membrane enzyme involved in respiration and metabolism. PNAS USA 9 (online Feb. 22,2008). C. Research Support NIH R01 GM/AI66466: PI J.I. Yeh 07/01/02-06/01/08 Structural Studies on Metabolic Membrane Proteins Major focus of this study is on the crystal structure determination of ...
... essential monotopic membrane enzyme involved in respiration and metabolism. PNAS USA 9 (online Feb. 22,2008). C. Research Support NIH R01 GM/AI66466: PI J.I. Yeh 07/01/02-06/01/08 Structural Studies on Metabolic Membrane Proteins Major focus of this study is on the crystal structure determination of ...
Cuvier meets Watson and Crick: the utility of molecules as classical
... The examples of tyrosine and lysine demonstrate what may be a common problem in biology-otherwise indistinguishable structures formed by different developmental pathways. What do we call homologous in this situation? While a molecule of tyrosine in a bacterium may not be homologous to a molecule of ...
... The examples of tyrosine and lysine demonstrate what may be a common problem in biology-otherwise indistinguishable structures formed by different developmental pathways. What do we call homologous in this situation? While a molecule of tyrosine in a bacterium may not be homologous to a molecule of ...
Two conformations of a crystalline human tRNA synthetasetRNA
... adding excess tryptophan and ATP, gave fully aminoacylated tRNATrp (Figure 1). When we added excess tryptophan and AMPPNP to the equimolar TrpRS and tRNATrp mixture, approximately 50% of tRNATrp was aminoacylated (Figure 1). (AMPPNP is an analog of ATP that has a phosphoramidate linkage between the ...
... adding excess tryptophan and ATP, gave fully aminoacylated tRNATrp (Figure 1). When we added excess tryptophan and AMPPNP to the equimolar TrpRS and tRNATrp mixture, approximately 50% of tRNATrp was aminoacylated (Figure 1). (AMPPNP is an analog of ATP that has a phosphoramidate linkage between the ...
Rice 6 Pages
... NS5B map to surface residues distant from the enzyme active sites, suggesting that these changes are likely to affect interactions between NS proteins and/or cellular factors. A striking feature of highly adaptive mutations is their tendency to decrease NS5A hyperphosphorylation, and many adaptive c ...
... NS5B map to surface residues distant from the enzyme active sites, suggesting that these changes are likely to affect interactions between NS proteins and/or cellular factors. A striking feature of highly adaptive mutations is their tendency to decrease NS5A hyperphosphorylation, and many adaptive c ...
m5zn_a9c640ccbe96115
... d) Quaternary 89) Mb is ………………….. structure of protein . a) Primary . b) Secondary . c) Tertiary . d) Quaternary . 90) All of the following is correct for Mb except . a) Have not allosteric effectors . b) Carry one molecules of oxygen . c) High affinity for oxygen . d) Quaternary . 91) Release of o ...
... d) Quaternary 89) Mb is ………………….. structure of protein . a) Primary . b) Secondary . c) Tertiary . d) Quaternary . 90) All of the following is correct for Mb except . a) Have not allosteric effectors . b) Carry one molecules of oxygen . c) High affinity for oxygen . d) Quaternary . 91) Release of o ...
A proteomic study of African elephant milk: Inter
... β-casein is a major component of casein proteins and it is the most hydrophobic casein, furthermore, it does not contain a cysteine and the sequence consists of 209 amino acid residues (Greenberg et al., 1984; Martin et al., 2003). In solution, β-casein forms detergent like micelle aggregates and th ...
... β-casein is a major component of casein proteins and it is the most hydrophobic casein, furthermore, it does not contain a cysteine and the sequence consists of 209 amino acid residues (Greenberg et al., 1984; Martin et al., 2003). In solution, β-casein forms detergent like micelle aggregates and th ...
Coordination Chemistry of cis,trans-1,3,5
... Cu(NO3)2, CuBr2, CuCl2, CuF2, CuSO4} leads to discrete diligand complexes. Protonation of the copper(II) complexes predominantly forms monoligand species, which can aggregate into higher nuclearity clusters. Protonated Cu(NO3)2 and CuBr2 complexes (HNO3 and HBr, respectively) remain mononuclear. CuC ...
... Cu(NO3)2, CuBr2, CuCl2, CuF2, CuSO4} leads to discrete diligand complexes. Protonation of the copper(II) complexes predominantly forms monoligand species, which can aggregate into higher nuclearity clusters. Protonated Cu(NO3)2 and CuBr2 complexes (HNO3 and HBr, respectively) remain mononuclear. CuC ...
Protein–protein interaction
Protein–protein interactions (PPIs) refer to physical contacts established between two or more proteins as a result of biochemical events and/or electrostatic forces.In fact, proteins are vital macromolecules, at both cellular and systemic levels, but they rarely act alone. Diverse essential molecular processes within a cell are carried out by molecular machines that are built from a large number of protein components organized by their PPIs. Indeed, these interactions are at the core of the entire interactomics system of any living cell and so, unsurprisingly, aberrant PPIs are on the basis of multiple diseases, such as Creutzfeld-Jacob, Alzheimer's disease, and cancer.PPIs have been studied from different perspectives: biochemistry, quantum chemistry, molecular dynamics, signal transduction, among others. All this information enables the creation of large protein interaction networks – similar to metabolic or genetic/epigenetic networks – that empower the current knowledge on biochemical cascades and disease pathogenesis, as well as provide putative new therapeutic targets.