Young Innovators 2009
... Purpose: The highly polymorphic organic transport protein, OCT1; SLC22A1 is involved in the translocation of the hydrophilic anti-diabetic drug metformin from the blood into hepatocytes. Since many drugs are inhibitors of the OCT1 [1], this may lead to drug-drug interactions at the OCT1. The risk of ...
... Purpose: The highly polymorphic organic transport protein, OCT1; SLC22A1 is involved in the translocation of the hydrophilic anti-diabetic drug metformin from the blood into hepatocytes. Since many drugs are inhibitors of the OCT1 [1], this may lead to drug-drug interactions at the OCT1. The risk of ...
enriched protein products of marine origin like the new components
... after 2–3 minutes). Boiled shrimp are taken out and immediately cooled in the refrigerator at 0°C for 10–15 min. Next, boiled shrimp are cut, rinsed, and homogenized for 3 minutes. The enzyme-modified shrimp biomass is produced by the hydrolysis of the frozen northern shrimp (Pandalus borealis) usin ...
... after 2–3 minutes). Boiled shrimp are taken out and immediately cooled in the refrigerator at 0°C for 10–15 min. Next, boiled shrimp are cut, rinsed, and homogenized for 3 minutes. The enzyme-modified shrimp biomass is produced by the hydrolysis of the frozen northern shrimp (Pandalus borealis) usin ...
Methods S1.
... the construction of P750-clpP1P2DAS was transformation of the attB L5 site integrating plasmid, pEXPR(kan)::RT38-p750-P1P2DAS into H37Rv/pNIT(zeo)::RecET-SacB to create a clpP1P2 merodiploid. The pEXPR(kan) plasmid was generated using multi-site gateway to insert the tetracycline-off repressor (RT38 ...
... the construction of P750-clpP1P2DAS was transformation of the attB L5 site integrating plasmid, pEXPR(kan)::RT38-p750-P1P2DAS into H37Rv/pNIT(zeo)::RecET-SacB to create a clpP1P2 merodiploid. The pEXPR(kan) plasmid was generated using multi-site gateway to insert the tetracycline-off repressor (RT38 ...
X-linked myopathies
... • Difficulty standing up • Muscle weakness starting from the lower limbs • Raised creatine kinase levels • Scoliosis • Dilated cardiomyopathy in adolescence ...
... • Difficulty standing up • Muscle weakness starting from the lower limbs • Raised creatine kinase levels • Scoliosis • Dilated cardiomyopathy in adolescence ...
Significance of Intestinal Digestion of Dietary Protein
... compared these values with true intestinal digestibility of proteins according to tabular values for 29 feeds. Tabular values were found to be generally higher than those estimated using the MBT (89.8 vs 84.4%). With both evaluation methods, feeds of plant origin seemed more digestible than those of ...
... compared these values with true intestinal digestibility of proteins according to tabular values for 29 feeds. Tabular values were found to be generally higher than those estimated using the MBT (89.8 vs 84.4%). With both evaluation methods, feeds of plant origin seemed more digestible than those of ...
chemical modification of carboxylic groups
... SDS. It seemed thus very likely that the numerous negative charges of the protein hindered an optimal fixation of SDS and that Actinomadura R39 /3-lactamase bound much less SDS per gram than do most polypeptides. On that basis, the remarkably low electrophoretic mobility of the Actinomnadura R39 /3- ...
... SDS. It seemed thus very likely that the numerous negative charges of the protein hindered an optimal fixation of SDS and that Actinomadura R39 /3-lactamase bound much less SDS per gram than do most polypeptides. On that basis, the remarkably low electrophoretic mobility of the Actinomnadura R39 /3- ...
Lab #8 Prelab: Protein, Triglycerides, and Esters Lab
... "Chemically, proteins are high polymers. They are polyamides, and the monomers from which they are derived are the α-amino carboxylic acids. A single protein molecule contains hundreds or even thousands of amino acids units; these units can be of twenty-odd different kinds. The number of different p ...
... "Chemically, proteins are high polymers. They are polyamides, and the monomers from which they are derived are the α-amino carboxylic acids. A single protein molecule contains hundreds or even thousands of amino acids units; these units can be of twenty-odd different kinds. The number of different p ...
Fruitful Collaboration of Physician/Physiologist and Engineer in the
... therapy to the ESRD patient. Working on these problems started with a traditional supplier-user relationship but evolved into a close collaboration to answer fundamental questions of physiology which has resulted in not just a rewarding professional career but a long personal friendship. I believe t ...
... therapy to the ESRD patient. Working on these problems started with a traditional supplier-user relationship but evolved into a close collaboration to answer fundamental questions of physiology which has resulted in not just a rewarding professional career but a long personal friendship. I believe t ...
OVERALL MECHANISMS OF QUINOLONE RESISTANCE
... strains possess urovirulence factors such as Pap, Hly, Cnf o Sat with a lower frequency • A decrease in type 1 fimbriae expression is also observed in some quinolone-resistant uropathogenic E.coli ...
... strains possess urovirulence factors such as Pap, Hly, Cnf o Sat with a lower frequency • A decrease in type 1 fimbriae expression is also observed in some quinolone-resistant uropathogenic E.coli ...
Water-Mediated Dimerization of Ubiquitin Ions Captured by
... means to directly capture the evaporative dynamics of biological molecules as they transition from solution to the gas phase. For example, cryo-IM-MS studies of protonated water clusters revealed distinct changes in the size and shape of ions as the H-bond network of water molecules develops from sm ...
... means to directly capture the evaporative dynamics of biological molecules as they transition from solution to the gas phase. For example, cryo-IM-MS studies of protonated water clusters revealed distinct changes in the size and shape of ions as the H-bond network of water molecules develops from sm ...
Document
... found in granules of mast cells, has a structure similar to that of heparan sulfates, but is more highly sulfated. When released into the blood, it inhibits clot (coagulation) formation by interacting with the protein antithrombin. Heparin has an extended helical conformation. ...
... found in granules of mast cells, has a structure similar to that of heparan sulfates, but is more highly sulfated. When released into the blood, it inhibits clot (coagulation) formation by interacting with the protein antithrombin. Heparin has an extended helical conformation. ...
Protein Metabolism
... The role of urea cycle “arg” has been discussed. A high protein diet increases the rate of synthesis of arginase in liver. Therefore, up-regulates the urea cycle. Notes: About 80% of nitrogen in human is excreted as urea, small amounts of ammonia, A.As., urate, creatinine and other nitrogenous ...
... The role of urea cycle “arg” has been discussed. A high protein diet increases the rate of synthesis of arginase in liver. Therefore, up-regulates the urea cycle. Notes: About 80% of nitrogen in human is excreted as urea, small amounts of ammonia, A.As., urate, creatinine and other nitrogenous ...
Changes in Typical Organelles in Developing Cotyledons of Soybean
... By 20 OAF plastids increased in number, especially in the outer portions of the seeds (Fig. 3B). The starch grains in plastids continued to increase in size and in number up to about 40 OAF (Fig. 3E) and then disappeared rapidly as seeds reached maturity (Fig. 3F). The formation of vacuoles smaller ...
... By 20 OAF plastids increased in number, especially in the outer portions of the seeds (Fig. 3B). The starch grains in plastids continued to increase in size and in number up to about 40 OAF (Fig. 3E) and then disappeared rapidly as seeds reached maturity (Fig. 3F). The formation of vacuoles smaller ...
Tutorial for PyMOL
... In this section you will get familiar with PyMOL. PyMOL is one of a few open source visualization tools available for use in structural biology. You can download it to your home computer ...
... In this section you will get familiar with PyMOL. PyMOL is one of a few open source visualization tools available for use in structural biology. You can download it to your home computer ...
The Frog Cell Cycle
... Funding for this workshop was provided by the program “Computational Modeling and Analysis of Complex Systems,” an NSF Expedition in Computing (Award Number 0926200). ...
... Funding for this workshop was provided by the program “Computational Modeling and Analysis of Complex Systems,” an NSF Expedition in Computing (Award Number 0926200). ...
Covalent interactions are not required to permit or stabilize the non
... Hepatitis C virus (HCV) encodes two glycoproteins, E1 and E2, which are thought to locate to the envelope of virus particles. These proteins form two complexes in tissue culture systems, a high molecular mass aggregate that contains intermolecular covalent bonds and a native complex in which E1 and ...
... Hepatitis C virus (HCV) encodes two glycoproteins, E1 and E2, which are thought to locate to the envelope of virus particles. These proteins form two complexes in tissue culture systems, a high molecular mass aggregate that contains intermolecular covalent bonds and a native complex in which E1 and ...
Biochemical and Molecular Genetics of Human Disease
... – “ apt to occur in several brothers and sisters whose parents do not exhibit the anomaly and direct transmission from parent to child is very rare” – In 8 of 17 families, parents were first cousins ...
... – “ apt to occur in several brothers and sisters whose parents do not exhibit the anomaly and direct transmission from parent to child is very rare” – In 8 of 17 families, parents were first cousins ...
Covalent interactions are not required to permit or stabilize the non
... Hepatitis C virus (HCV) encodes two glycoproteins, E1 and E2, which are thought to locate to the envelope of virus particles. These proteins form two complexes in tissue culture systems, a high molecular mass aggregate that contains intermolecular covalent bonds and a native complex in which E1 and ...
... Hepatitis C virus (HCV) encodes two glycoproteins, E1 and E2, which are thought to locate to the envelope of virus particles. These proteins form two complexes in tissue culture systems, a high molecular mass aggregate that contains intermolecular covalent bonds and a native complex in which E1 and ...
Narcissus pseudonarcissus L. `Dutch Master`
... tepals of D1 and D4 flowers confirmed that many of the sequences (or closely related homologs) were more highly expressed in D4 flowers (data not shown). These sequences are indicated in Table 1 by an asterisk. Conventional RNA gel blot analysis was used to examine the time course of upregulation of ...
... tepals of D1 and D4 flowers confirmed that many of the sequences (or closely related homologs) were more highly expressed in D4 flowers (data not shown). These sequences are indicated in Table 1 by an asterisk. Conventional RNA gel blot analysis was used to examine the time course of upregulation of ...
Dynamic properties of a reconstituted myelin sheath
... often polyanionic in nature [2]. Due to its high charge and low overall hydrophobicity, there is significant intramolecular electrostatic repulsion in MBP; this results in MBP being an extended, intrinsically unstructured protein in solution [12,21]. P2 is a small folded protein with a high homology ...
... often polyanionic in nature [2]. Due to its high charge and low overall hydrophobicity, there is significant intramolecular electrostatic repulsion in MBP; this results in MBP being an extended, intrinsically unstructured protein in solution [12,21]. P2 is a small folded protein with a high homology ...
Amino Acids
... 4- NH2 group or nitrogen act as a base (basic amino acids ): as lysine, arginine and histidine 5- COOH group ( acidic amino acids): as aspartic and glutamic . B- Non polar amino acids: R is alkyl hydrophobic group which can’t enter in hydrogen bond formation. 9 amino acids are non polar ( glycine, a ...
... 4- NH2 group or nitrogen act as a base (basic amino acids ): as lysine, arginine and histidine 5- COOH group ( acidic amino acids): as aspartic and glutamic . B- Non polar amino acids: R is alkyl hydrophobic group which can’t enter in hydrogen bond formation. 9 amino acids are non polar ( glycine, a ...
Amino acids 1
... hydrophobic surfaces of these side chains. Water is very happy in bulk water because there it has on average 3.6 H-bonds and about six degrees of freedom. So, whenever we discuss protein structure, folding, and stability, it is all the entropy of water, and that is called the hydrophobic effect. ...
... hydrophobic surfaces of these side chains. Water is very happy in bulk water because there it has on average 3.6 H-bonds and about six degrees of freedom. So, whenever we discuss protein structure, folding, and stability, it is all the entropy of water, and that is called the hydrophobic effect. ...
Overcoming stalled translation in human mitochondria
... is essential. What happens when a translating ribosome stalls on a messenger RNA? Many highly intricate processes have been documented in the cytosol of numerous species, but how does organellar protein synthesis resolve this stalling issue? Mammalian mitochondria synthesize just thirteen highly hyd ...
... is essential. What happens when a translating ribosome stalls on a messenger RNA? Many highly intricate processes have been documented in the cytosol of numerous species, but how does organellar protein synthesis resolve this stalling issue? Mammalian mitochondria synthesize just thirteen highly hyd ...
Yeast Display Evolution of a Kinetically Efficient 13-Amino
... difficult to recapitulate in a peptide. Second, the function may require contributions from multiple, noncontiguous regions of a protein. Third, structural information is not available for many proteins, and in some cases, even the regions that contribute to a protein’s relevant activity are not kno ...
... difficult to recapitulate in a peptide. Second, the function may require contributions from multiple, noncontiguous regions of a protein. Third, structural information is not available for many proteins, and in some cases, even the regions that contribute to a protein’s relevant activity are not kno ...
Protein–protein interaction
Protein–protein interactions (PPIs) refer to physical contacts established between two or more proteins as a result of biochemical events and/or electrostatic forces.In fact, proteins are vital macromolecules, at both cellular and systemic levels, but they rarely act alone. Diverse essential molecular processes within a cell are carried out by molecular machines that are built from a large number of protein components organized by their PPIs. Indeed, these interactions are at the core of the entire interactomics system of any living cell and so, unsurprisingly, aberrant PPIs are on the basis of multiple diseases, such as Creutzfeld-Jacob, Alzheimer's disease, and cancer.PPIs have been studied from different perspectives: biochemistry, quantum chemistry, molecular dynamics, signal transduction, among others. All this information enables the creation of large protein interaction networks – similar to metabolic or genetic/epigenetic networks – that empower the current knowledge on biochemical cascades and disease pathogenesis, as well as provide putative new therapeutic targets.